Objective Our aim was to observe the effects of prenatal nicotine exposure on development of cerebral cortex and hippocampus in the offsprings of pregnant mice and expressions of NCAM proteins. Methods We established prenatally nicotine exposed(PNE) animal models.We investigated the offspring's structural changes of cerebral cortex and hippocampus with HE staining.Using immunohistochemistry and Western blotting we observed the changes of expressions of NCAM proteins in the neonatal mice. Results Cerebral cortex and hippocampus as well as hippocampal cell layers of the PNE offsprings were thinner than that of the control group.The expressions of PSA-NCAM in cerebral cortex and hippocampus of the PNE offsprings were higher than the control group while the expressions of NCAM-180,140 and 120 weaker.Conclusion Prenatally nicotine exposure can prevent normal morphogenesis of cerebral cortex and hippocampus of mice and can change the expressions of NCAM proteins in cerebral cortex and hippocampus of neonatal mice.

Objective: The aim of this study was to analyze the health-related quality of life (HRQoL) and their influencing factors in patients with asymptomatic unruptured intracranial aneurysms (UIAs) after endovascular treatment. Methods: We retrospectively reviewed 44 patients with asymptomatic UIAs (56aneurysms) received endovascular treatment in Xuanwu Hospital of Capital Medical University from January 2015 to May 2017. The average follow-up time was (28 ±9) month. The clinical data of patients were collected, and the HRQoL was evaluated by the SF-36 questionnaire. The SF-36 results and influencing factors were analyzed. Results: Forty-four patients with UIAs in this study showed significantly lower body pain,physical function,and mental health in the three dimensions of the SF-36 scale than the normal population in China (75 ± 15,82 ± 15 and 71 ± 14 vs. 83 ±20,88 ± 17 and 79 ± 15;t =2.89,2. 14 and 3. 34 Respectively; all P < 0. 05). The results of univariate analysis showed (hat the Mental Component Summary score (MCS) of patients with sleep duration < 6 h and ≥6h were respective 63 ± 18 and 79 ± 14points (t = -2.41);the MCS score of patients with disease duration ≤3 months and > 3 months were respective 76 ±15 and 89 ± 7 points (t = -2. 11); the differences were significant (both P < 0. 05). The physical component summary score (PCS) of sleep duration < 6 h and ≥ 6 h were respective 63 ± 22 and 77 ± 17 (t = - 2. 11); the PCS of ≤3 months and > 3 months were respective 73 ± 17 and 90 ± 5 (l = -2.74);the differences were significant (both P <0.05). Multivariate analysis showed that the course of disease ≤ 3 months was the independent risk factor of PCS (95% CI 2. 23 -27. 42, P = 0. 02), and sleep time<6h was the independent risk factor of MCS (95% CI 1. 44 -28. 92, P = 0. 03). 12.5% (4/32) of these patients failed to recover to normal work before treatment even after long-term recovery (28 ± 9 months). Conclusions: HRQoL results in patients with asymptomatic UIAs after endovascular treatment were lower than those in the general population in multiple dimensions of SF-36. The course of disease ≤3 months and daily sleep time < 6h are independent risk factors for PCS and MCS,respectively.

Objectives: To investigate the morphological characteristics of pericallosal artery aneurysms (PAAs) and to analyze the morphological influence factor of PAA rupture. Methods: From January 2013 to May 2017A,40 consecutive patients with PAA admitted to the Department of Neurosurgery,Xuanwu Hospital, Capital Medical University were enrolled retrospectively. They were diagnosed by whole brain vascular DSA before treatment. Forty patients were divided into ruptured group (n = 22 with 22 aneurysms) and the unruptured group (n = 18 with 23 aneurysms). The morphological parameters of the number of aneurysms in two-dimensional or three-dimensional rotational angiography included diameter, height, width, aneurysm neck width,diameter of parent arteries, and inflow angle of aneurysms, and the aspect ratio, size ratio and length-width ratio (aneurysm diameter/aneurysm width) of each aneurysm were further calculated. The aspect ratio was calculated using aneurysm height/aneurysm neck width and aneurysm diameter/aneurysm neck width, respectively, and the size ratio was calculated using aneurysm height/parent artery diameter and aneurysm diameter/parent artery diameter, respectively. The differences of morphological parameters of PAAs were compared and multivariate logistic regression analysis was performed to preliminarily investigate the morphology of influencing factors of PAA rupture. Results: (1) There were no significant differences in age, male, multiple aneurysms, and risk factors for cardiocerebrovascular diseases between the two groups (all P>0.05). (2) The proportion of irregular aneurysms and the length-width ratio of aneurysms in the rupture group were higher than those in the unruptured group. The difference between the groups was statistically significant (90.9% [20/22] ts. 39. 1% [9/23],χ2 =8. 01); 1. 35 ±0. 36 vs. 1. 01 ±0. 22,t= -3. 85; all P <0. 01). There were no significant differences in aneurysm diameter,height,width,diameter of parent artery, aspect ratio, inflow angle, aneurysm neck, and size ratio between the two groups (all P > 0. 05). (3) Amongthe 45 aneurysms,the proportion of A3 anterior bifurcation aneurysms was 53.3% (24 aneurysms), and the proportion of the ruptured group and unruptured group was 68. 2% (15/22) and 39. 1% (9/23) respectively. There were no significant differences between the groups (χ2 =0. 381,P = 0. 051). (4) The mean length-width ratio in 45 PAAs was 1. 18. using this as the defined value, the length-width ratio was converted into a two-category variable,and the PAA rupture was used as the dependent variable. The univariate analysis of the morphological parameters in the length-width ratio (> 1. 18) and the irregular aneurysm were included in the multivariate logistic regression analysis. The results showed that the length-width ratio was >1.18 (OR,1.84,95% CI 1.42-28. 11,P =0. 016),and irregular aneurysms (OR,2. 06,95% CI 1. 66-37. 11, P = 0. 009) were the independent risk factor for PAA rupture. Conclusions: The ratio of height-length-width of PAAs and the irregular morphology of aneurysms suggest that PAAs are prone to rupture. The evaluation of PAA morphological parameters should be emphasized in clinical practice.

Objective:To investigate the status of work performance among rural doctors in Shanxi province and the factors related to their performance.Methods:From July to September 2019, 528 rural doctors in Shanxi province were selected by multi-stage stratified cluster sampling for the survey. Their job competency, job performance, psychological capital and burnout were evaluated. The structural equation model was used to construct the job performance analysis based on post competency.Results:The total score of post competency of rural doctors in Shanxi province was (188.6±20.3), and the total score of job performance was (71.3±11.5), both of which were in the middle level. The structural equation model was well fitted [goodness-of-fit index (GFI)=0.958, normal fix index (NFI)=0.920, adjusted goodness-of-fit index (AGFI)=0.910, root mean square error of approximation (RMSEA)=0.076]. Post competency, psychological capital and job burnout had direct effects on job performance ( P<0.05), with influence coefficients of 0.64, 0.35 and -0.25, respectively. Job competency indirectly affected job performance through psychological capital and job burnout ( P<0.05). Conclusion:The study suggest that higher post competency would facilitate to improve the work performance of rural doctors.

One hundred patients were diagnosed with coronary heart disease through dual-source CT coronary angiography (DSCTA) in our hospital,including 53 cases aged ≥60 y (elderly group) and 47 cases ＜60 y (middle-aged group).The curved-planar reconstruction (CPR),maximal intensity projection (MIP) and other post-processing reconstruction techniques were used to evaluate the indicators and differences of coronary stenosis ≥ 50％ ; the X-ray coronary angiography (CAG) findings were regarded as the gold standard.The evaluable rates of coronary arteries through DSCTA were 97.0％ and 98.6％ for two groups (P ＞0.05).The positive rate of coronary artery stenosis ≥ 50％ in elderly group was higher than that of middle-age group ( 12.6％ vs 9.0％ P ＜0.05 ).The rates of multi-vessel disease in elderly group (28.3％ ) was also significantly higher than that of middle-age group (6.4％,P ＜0.05 ).The sensitivity,specificity,positive predictive value and negative predictive value in evaluation of coronary artery stenosis ≥ 50％ for elderly patients were 92.3％,98.0％,88.0％ and 98.9％ respectively; those for middle-aged patients were 96.0％,99.0％,90.6％ and 99.6％ respectively ( P ＞0.05 ).DSCTA imaging provides an effective screening and evaluation method for coronary heart disease in elderly and middle-aged patients.

Objective To evaluate the role of different kinds of body positon in the stomach CT scanning.Methods It included 30 normal adults who underwent CT scanning in four differnent kinds of body positions.The relationship between the body position and the image appearence in the each part of stomach has been analysed.Results The results showed that the adjusting of body position was useul in revealing gastric wall and perigastric organs.The best imaging result of fundus ventriculi part of stomach can be obtained with the head down and the feet high position while patients lie on his back.The best body position to reveal corpus ventriculi part is in prone position and the best body position to reveal pars pylorica part is in right lateral position.There is a very significant difference between supine position and the best revealing position of each part of stomach prespectively(?

OBJECTIVE To investigate the etiology of acute respiratory tract infection(ARI)in Hefei area and risk factors that may influence the distribution of common pathogens.METHODS Direct immunofluorescence assay was applied to detect the respiratory syncytial virus(RSV),adenovirus(ADV),influenza virus A(FluA),influenza virus B(FluB),parainfluenza virus PIV(1,2,3)and real time fluorescent quantitation polymerase chain reaction (FQ-PCR)was applied to measuring Mycoplasma pneumoniae(MP)and Chlamydia pneumoniae(CP)in nasopharyngeal secretions and sputum specimens.RESULTS Among 530 cases included in this study,421 cases (79.43%)showed positive viral etiology.ADV accounted for 73(13.77%),FluA-68(12.83%),FluB-56 (10.56%),RSV-48(9.05%),PIVl-47(8.86%),PIV3-42(7.92%),PIV2-33(6.22%),MP-32(6.03%)and CP-22(4.15%).The detected positive rate of pathogens isolated in winter was the highest(85.07%).CONCLUSIONS Acute upper respiratory tract infection(AURTI)is common and more than 75%pathogens in Hefei area are virus in which the most commonly virus is ADV.Meanwhile atypical pathogens of infection should not be ignored.

Objective To establish a rapid assay for the determination of methotrexate (MTX) in serum.Method The assay was based on the ultraviolet absorbance of methotrexate at 306 nm. The separation of the drug was done by high performance capillary electrophoresis (HPCE). The bare fused-silica capillary was 60 cm in total length, 50.5 cm in efficient length and 75?m in diameter. The voltage of 25 kV was applied. The running buffer was 75 mmol/L phosphate, pH7.4. The performance of methodology was evaluated.Result The complete separation of MTX was achieved within 10 min. The linearity of the assay was from 1.1 ?mol/L to 1100.0 ?mol/L. The minimal detection limit was 0.55 ?mol/L.The recovery of MTX was from 88.2% to 98.2%. Within-run precision was 4.2% and between-run precision was ~5.4%. Conclusion The result indicated that the method was an effective method for clinical and scientific research with advantages of rapidity, simplicity and accuracy.

Objective:To evaluate the role of using the double body position CT(DBP-CT)in the staging of advanced gastric cancer.Methods:We performed CT examination in 85 patients with advanced gastric cancer which were divided into the double postion group(55 cases)and the single position group(30 cases),after oral intake water agent.The preoperative CT findings of each group was compared with surgical findings respectively and the accuracy in the CT staging of advanced gastric cancer of each group was estimated.Results:The accuracy in the preoperative CT staging of double position group was 89.09%,and in single postition group was 60%.There was a significant difference(P＜0.05).Conclusion:Our findings show that the DBP-CT is surperior to the single position group in finding lesion,confineing the range of the tumour,and determing the degree of adjacent invasion.It can improve the diagnostic accuracy in the preoperative staging of advanced gastric cancers significantly.

Objective To compare the dosimetry between three -dimensional conformal radiotherapy (3DCRT)and intensity -modulated radiotherapy(IMRT)in the treatment of upper thoracic esophageal carcino-ma,and to provide references to choose radiotherapy program for clinical physician .Methods twenty-five cases with upper esophageal carcinoma (clinical stageⅠ~Ⅲstage)were treated by 3DCRT and IMRT at the concentra three-dimensional radiation treatment planning system .The different exposure doses between target area and effected organs were compared by dose volume histogram ( DVH) with the planed target volume ( PTV) ,which must reach 95% of the prescriptive doses.Results Two different radiotherapy plans of IMRT and 3DCRT:V95, (99.91 ±0.14)%,(95.73 ±4.14)% respectively,P<0.05;targeting maximum doses(Dmax)were(6658.26 ±215.29)cGy,(6 664.20 ±465.16)cGy,P>0.05;targeting minimum dose(Dmin)were(5 458.88 ±184.06) cGy,(4541.60 ±599.0)cGy,P<0.05;targeting average doses(Dmean)were(6 232.80 ±53.00)cGy and (6 105.78 ±163.34)cGy,P<0.05.CI values were (0.76 ±0.04) and(0.57 ±0.05),P<0.05;HI values were(1.07 ±0.02) and(1.12 ±0.06),P <0.05;Spinal cord maximum dose (3 889.68 ±712.69) cGy, (4 337.48 ±178.49)cGy,P<0.05;Lung V20(20.94 ±5.32)%,(21.90 ±6.94)%,P>0.05;Lung V10 (35.39 ±11.41)%,(29.0 ±8.80)%,P<0.05,Lung V5(44.95 ±15.55)%,(37.27 ±11.93)%,P<0.05. Conclusion Intensity-modulated radiotherapy is better than 3DCRT technology in showing PTV volume ,target conformal degrees and the mean index ,spinal cord protection ,However ,The risk of lung injury could be increased with the enlarged area of low -dose irradiation in lung .