OBJECTIVE:To investigate the compatible stability of cefepime dihydrochloride mixed with total parenteral nutrient(TPN). METHODS:Cefepime dihydrochloride mixed with TPN at room temperature for 24 h. The appearances of the mixture were observed at 0,2,4,6,8,10,24 h and the change of pH value was evaluated. The contents of cefepime dihydrochloride were determined by HPLC. RESULTS:No change was observed in the contents of cefepime dihydrochloride in total parenteral nutrient within 24 h. There was no significant change in the appearance,color and pH value of nutrient. CONCLUSION:At room temperature cefepime dihydrochloride in TPN is stable within 24 h.

Objective To investigate the correlation of serum adiponectin (APN),C-reactive protein (CRP) levels and carotid atherosclerosis plaque in patients with acute cerebral infarction (ACI).Methods Sixty patients with ACI (ACI group) were diagnosed by head CT and MRI examination.And sixty cases of healthy check-up were as control group.The serum APN and CRP were examined by double antibody sandwich enzyme linked immunosorbent assay method,and carotid atherosclerosis was examined by ultrasound.Results The serum APN and CRP levels in ACI group were (4.36 ± 2.54),(8.93 ± 2.89)mg/L,and in control group were (8.43 ± 3.51),(1.12 ± 0.52) mg/L.The serum APN level in ACI group was significantly lower than that in control group,and the serum CRP level in ACI group was significantly higher than that in control group (P ＜ 0.05).In ACI group,the serum APN level after treatment was significantly increased compared with that before treatment,the serum CRP level after treatment was significantly decreased compared with that before treatment.The differences were (6.44 ± 2.31) and (4.10 ± 0.72) mg/L respectively.The rate of carotid atherosclerosis in ACI group was 78.33％ (47/60),and the rate of carotid atherosclerosis plaque was 71.67％(43/60).The stable plaque was in 13 cases and instability plaque was in 30 cases.The rate of carotid atherosclerosis in control group was 26.67％(16/60),and the rate of carotid atherosclerosis plaque was 13.33％(8/60).The stable plaque was in 6 cases and instability plaque was in 2 cases.The differences between the two groups had statistical significance (P ＜ 0.05).In ACI group,the serum APN and CRP levels in ACI without carotid atherosclerosis plaque were (6.89 ± 2.37) and (5.41 ± 1.38)mg/L,in ACI with carotid atherosclerosis plaquewere (3.28 ± 1.99) and (9.42 ±3.70) mg/L.Carotid atherosclerosis plaque and serum APN level showed negative correlation (r =-0.76,P ＜ 0.05),and serum CRP level was positively correlated (r =0.81,P ＜ 0.05).The serum APN and CRP levels in ACI with stable plaque were (4.32 ± 2.14) and (6.65 ± 2.69) mg/L,in ACI with instability plaque were (2.11 ± 1.35) and (10.11 ± 3.82) mg/L.Carotid artery plaque stability and serum APN level showed negative correlation (r =-0.69,P ＜ 0.05),and serum CRP level was positively correlated (r =0.77,P＜ 0.05).Conclusions The occurrence of ACI is closely related to carotid atherosclerotic plaque.The serum APN level in ACI patients is significantly lower than that in normal crowd,but serum CRP level in ACI patients is significantly higher than that in normal crowd.The rate of carotid atherosclerosis is significantly increased,most of which is instability plaque.Carotid atherosclerosis plaque and serum APN level has negative correlation,and serum CRP level is positively correlated.Carotid artery plaque stability and serum APN level has negative correlation,and serum CRP level is positively correlated.

At present, coronary stent has been extensively used in clinic. It can significantly improve acute obstruction complication and vascular restenosis following percutaneous transluminal coronary angioplasty. However, restenosis also takes place after stent implantation, in particular for complex pathological changes. It has been demonstrated that thrombus, inflammation, and smooth muscle hyperplasia are three important stages for in-stent restenosis, and vascular intima hyperplasia is the major mechanism. After retrieving related articles, this paper discussed biocompatibility and restenosis of metal stent and drug-eluting stent, analyzed influence of stent implantation on collateral circulation function and inflammatory reactions, and explored the application of biodegradable stent.

Objective To characterize the clinical features of patients with high level of carbohydrate antigen (CA)724 in order to facilitate the recognition of this clinical manifestations.Methods Eight-two cases of hospitalized patients diagnosed with gout were selected and divided into 2 groups according to the level of CA724,and the clinical features and laboratory indexes were compared between the 2 groups.T test was used in the numerical variable data between groups,x2 test was used in the categorical variable data.Correlation analysis was tested using Spearman analysis.Results The incidence of gout stones in the group with high level of CA724 was 61％,which was significantly higher than that in the group of normal CA724 of 28％.There were statistically significant differences between the two groups (x2=8.894,P＜0.05).The incidence of kidney stones in the group with high level of CA724 was 65％,which was significantly higher than that in the group of normal CA724 of 22％.There were statistically significant differences between the two groups (x2=15.013,P＜0.05).But there were no statistically significant differences in age of onset,blood uric acid,erythrocyte sedimentation rate,and serum creatinine between the two groups (P＞0.05).Conclusion The significantly elevated level of CA724 canappeare in gout patients,But it isnotcorrelated with tumor,which should be paid attention by clinician.It helps to avoid unnecessary checks and reduce the psychological and financial burden of patients.

Since human imprinted genes in 11P15.5 was found,it had been proved that various human physiological abnormality associated with it.As one of the first identified imprinted non-coding RNA genes,more attention is paid to lncRNA H19 by many scholars not only because it's linked with Beckwith-Wiedemann syndrome (BWS) and Russell syndrome (SRS),but also H19 gene is the only imprinted gene belonging to long non-coding RNA related with a variety of tumors which can also produce microRNA675 and lncRNA 91H coming from H19 gene antisense-coding.This paper will review possible mechanisms of H19 gene in tumorigenesis process and its research status in Cancer therapy from four aspects:imprinting feature,single nucleotide polymorphisms,lncRNA character and its possible action signal axis in cancer.

OBJECTIVE: To evaluate the efficacy and safety of recombinant human Ad-p53 injection (rAd-p53) combined with cisplatin in the treatment of malignant pleural effusions of non-small-cell lung cancer (NSCLC). METHODS: 46 patients with malignant pleural effusions of NSCLC were randomly divided into groupⅠ, Ⅱand Ⅲ. The groups were given cisplatin, rAd-p53 and combination medication, respectively once a week. The efficacy and quality of life were evaluated after 4 weeks. RESULTS: There were significant difference in among three groups in effective rate and improvement rate of life quality (P0.05). CONCLUSION: rAd-p53 combined with cisplatin has sound effect on malignant pleural effusions of NSCLC without aggravating effect on the toxicity of chemotherapy, which is worth of spreading in the clinic.

OBJECTIVE: To investigate the development of clinical pharmacy according to the publication number of literatures in pharmacy periodicals in China. METHODS: General keywords, such as rational administration, pharmaceutical care, training for clinical pharmacist, and special keywords, such as drug utilization, administration analysis, therapeutic drug monitoring(TDM), adverse drug reaction monitoring, medication history, pharmacoeconomic analysis, medicine consultation, were selected for retrieving related literatures from CBM database from 1994 to 2008. Literatures retrieved were analyzed statistically. RESULTS: Clinical pharmacy developed rapidly relatively in recent years. The number of literatures which were retrieved using general keywords was 12 and 15 for using special keywords in 1994. The number of literatures was increases year by year and up to 1 117 and 329 in 2008. However, development orientation of literatures was unbalanced. Literature about rational administration, drug utilization and administration analysis constituted a high proportion while development of clinical pharmacy should be enhanced in respect of clinical pharmacist training, medication history writing, medicine consultation and adverse drug reaction monitoring. CONCLUSION: Disadvantage in disequilibrium of development should be changed as long as clinical pharmacy develops rapidly in order to promote its overall development.

Objective:To investigate the influence of gradually induced occlusion disorder on temporomandibular joint in young rabbits. Methods: The upper first premolar on one side and the lower first premolar on the other side of 18 New Zealand white rabbits at the age of 7 months, were pulled forward with the orthodontic string, making them inclined medially to the anterior intrinsic space. The TMJs were removed 1,2 and 3 months respectively after set up and observed histologically. Results: In the 1 month group,TMJs exhibited regressive remodeling on the lateral side. In the 2 month group,the joints simultaneously showed progressive remodeling and degenerative changes.The TMJ condyle cartilage were thinner than the controls.Conclusion: Gradually induced occlusion disorders can lead TMJ degenerative changes in young rabbit.

The diagnosis and treatment of age-related macular degeneration (AMD) is an international hotspot of eye research.Successful clinical applications of anti-VEGF drugs promoted both basic research and clinical practice of AMD.A number of countries and professional societies have established clinical guidelines for AMD management,including the epidemiology,risk factors,diagnosis,classification,and treatment process.These AMD guidelines are mostly based on recently published results of clinical trials,provided good model of evidence based medicine.It is urgent and necessary to have our own guideline which is suitable for Chinese patients.Reviewing and learning existed guidelines will help us to improve the clinical practice of AMD in China.

Objective To investigate the expression of heme oxygenase-1 (HO-1) in type 2 diabetes mellitus (T2DM) as well as hidden diabetes mellitus (HDM) and explore its clinical significance.Methods A total of 384 suspected patients with T2DM having underwent oral glucose tolerance test were enrolled in this study:216 cases of patients were T2DM (T2DM group),and 168 cases of patients were HDM (HDM group).T2DM patients were divided into single-vessel lesion group(122 cases),double-vessel lesion group(54 cases),and more-vessel lesion group(40 cases).Another 60 healthy person were as control group.The intima-media thickness (IMT) of carotid was measured by ultrasonography.The level of serum HO-1 was detected with enzyme-linked immunosorbent assay.Results The serum level of HO-1 in control group,HDM group and T2DM group was (1.24 ± 0.53),(2.12 ± 0.84),(3.46 ± 1.23) μ g/L.The serum level of HO-1 in T2DM group was significantly higher than that in control group and HDM group (P ＜ 0.05),and in HDM group it was significantly higher than that in control group(P ＜ 0.05).There were significant differences in the serum level of HO-1 and IMT of carotid among single-vessel lesion group,double-vessel lesion group,and more-vessel lesion group [(2.94 ± 1.14),(3.72 ± 1.36),(4.64 ± 1.58) μg/L and (1.21 ±0.16),(1.44 ± 0.20),(1.62 ± 0.27) mm] (P ＜ 0.05).The serum level of HO-1 and IMT of carotid in double-vessel lesion group was higher than that in single-vessel lesion group,and in more-vessel lesion group it was higher than that in double-vessel lesion group and single-vessel lesion group (P＜ 0.05 or ＜ 0.01).The serum level of HO-1 was positively related with IMT of carotid (r =0.512,P ＜0.01).Conclusion The high-level expression of HO-1 is found in HDM and T2DM patients,which maybe play a key role in the early diagnosis of T2DM.