Objective To observe the expression of intercellular adhesion molecule 1(ICAM-1)and vascular cellular adhesion molecuh 1(VCAM-1)in placenta in patients with hypertensive disorders in pregnancy,investigate their roles in patients with hypertensive disorders in pregnancy and correlation.Methods Fifty-two patients with hypertensive disorders in pregnancy(disease group)were collected,including 11 csses with gestational hypertension(GH),18 cases with mild pre-eelampsia(MP)and 23 cases with gevere pre-eclampsia(SP),26 cases of normal pregnant women as control group.Immunohistochemistry and image analytical technique were employed to show the expression of ICAM-1 and VCAM-1.Results The quantities of ICAM-1 and VCAM-1 expressed in the placental vascular endothelial cell were 78.85%(41/52)and 75.00%(39/52)in disease group,26.92%(7/26)and 23.08%(6/26)in control group,there was a significant difference between the two groups(P<0.01).and those in SP patients[100.00%(23/23)and 100.00%(23/23)]were higher than those in MP patients[77.78%(14/18)and 72.22%(13/18)]and GH patients[36.36%(4/11)and 27.27%(3/11)](P<0.05 or<0.01).The quantities of ICAM-1 and VCAM-1 expressed in the placental villous syncytlotrophoblast were cell 38.46%(20/52)and 44.23% (23/52)in disease group,100.00%(26/26)and 96.15%(25/26)in control group,there was a significant difference between the two groups(P<0.01),and those in SP patients[8.70%(2/23)and 13.04%(3/23)]were lower than those in MP patients[44.44%(8/18)and 50.00%(9/18)]and GH patients[90.91%(10/11)and 100.00%(11/11)](P<0.05 or<0.01).Conclusion Abnormal expression of ICAM-1 and VCAM-1 in the placental vascular endothelial and villous syncytiotrophoblast cell in patients with hypertensive disorders in pregnancy may participate in pathogenic process of vascular endothelial damage of hypertensive disorders in pregnancy.

Object To develop a method for the determination of flavonoids in Hypericum perforatum L processed by different drying methods to provide a basis for the processing of the natural herbs Methods The chromatographic conditions were Discovery C 18 column (5 ?m, 4.6 mm ? 25 cm). detection wavelength 365 nm; mobile phrase : water : acetonitrile : phosphoric acid (825∶175∶1); flow rate 1.0 mL/min Results The contents of rutin and hyperin were at their maximum when dried at 60 ℃ for 4 h Rutin had a good linearity in the range of 0.107～2 675 ?g, average recovery rate 99 32%, RSD 1 007%, and hyperin in the range of 0 107～2 675 ?g, average recovery rate 99 54%, RSD 3 591% Conclusion Temperature is the main factor influencing the content of flavonoids The HPLC determination was shown to be rapid, reliable and simple, and may be used for the quality control of H perforatum

AIM To investigate the effects of levo phenoprolamine hydrochloride [ levo 1 (2,6 dimethylphenoxy) 2 (3,4 dimethoxyphenyl ethylamino) propane hydrochloride] [ l DDPH([?]D 25 1 08)]on experimental arrhythmias. on experimental arrhythmias. METHODS Intravenous administration of ouabain, aconitine or CaCl 2 induced arrhythmias in rats or guinea pigs; Microelectrode recording was used to record action potential; Whole cell patch clamp technique was used to record L type calcium current ( I Ca,L ). RESULTS ① l DDPH 50 mg?kg -1 inhibited the ventricular arrhythmias induced by intravenous injection of ouabain in guinea pigs or aconitine and CaCl 2 in rats. ② l DDPH 30 ?mol?L -1 shortened 50% action potential duration (APD 50 ) and prolonged effective refractory period (ERP) ( n =6, P

Objective To study the ultrasound-guided treatment by real-time biplane imaging in animals and patients. Methods In 6 dogs gallbladders were substituted as hepatic cysts. The shapes of gallbladders and spatial positions of needle tips in gallbladders were studied by real-time biplane imaging before and during treatments. During treatments of 3 renal cysts the changes of cysts and the spatial relations between needle tips and renal cysts were observed to confirm that the needle tips were always in the cysts. In 2 cases of hepatocellular carcinoma boiloff of the tissue around the antenna was observed by real-time biplane imaging during microwave coagulation. Results It was rapid and convenient to acquire the shape information of objects and locate the position of puncture by real-time biplane imaging. And it was more convenient to observe the spatial relation between needle tips and objects of treatment than by conventional single-plane imaging. Conclusions The method of real-time biplane imaging has great advantages in ultrasound-guided treatment.

Objective To analyze the diagnostic process of a rare case of acute myeloid leukemia with minimal differentiation undergoing a lineage switch to mixed phenotype acute leukemia, NOS-rare types,and to investigate its difference from other acute myeloid leukemia and mixed phenotype acute leukemia. Methods Following tests were performed on the patient with switched mixed phenotype acute leukemia and three control leukemia patients ( including two acute myeloid leukemia with minimal differentiation and one mixed phenotype acute leukemia ). Cell morphology was analyzed by bone marrow smear and related cell chemical staining. Immunophenotyping of bone marrow was performed by flow cytometry ( FCM ). G-banding technique was used for karyotype analysis and RT-PCR was used for fusion gene detection. All the laboratory data of the switched patient were compared to that of three control patients in order to reveal the characteristics of such a rare phenotype switch in acute leukemia. Results Before switching, the morphology of acute myeloid leukemia with minimal differentiation demonstrated 0.82 blasts occurring in bone marrow, distinct nucleoli and absence of Auer rods. Blast cells expressed hematopoieticassociated antigens ( CD38, HLA-DR ), myeloid antigens ( CD13, CD56, CD11b ) and CD7. And these blasts were negative for MPO, CD33, CD15, CD79, CD19, CD22, cytoplasmic CD3, CD4 and CD8. After switching, 0. 42 blasts were found in bone marrow, showed eosinophilia and presence of basophile. Blast cells expressed hematopoietic-associated antigens ( CD38, HLA-DR ), myeloid antigens ( MPO, CD13 ),lymphoid antigens ( CD19, CD79a ,cytoplasmic CD3, and CD7 ). The control group showed typical morphology and immunophenotyping. No abnormal karyotype and fusion gene were detected. Conclusions It is a rare and complicated case that acute myeloid leukemia with minimal differentiation switched to mixed phenotype acute leukemia, NOS-rare types. The laboratory features, especially the change of immunophenotyping play an important role in the diagnosis.

The distribution of years, active regions, high production authors and core journals in papers on saffron crocus was analyzed using HistCite.The main methods in research of saffron crocus were described according to its chronological chart generated by the Pajek-generated matrix with its development history revealed.

Objective To explore the short- term efficacy of argon- helium knife cryotherapy followed by chemotherapy in treating advanced non- small cell lung cancer (NSCLC) and to investigate its effect on the long- term survival. Methods During the period from March 2005 to March 2008, a total of 61 patients withⅢb or Ⅳ stage NSCLC received argon- helium knife cryotherapy followed by chemotherapy (study group), and other 52 patients with Ⅲb or Ⅳ stage NSCLC were treated with chemotherapy only (control group). The clinical data were retrospectively analyzed. The pain was evaluated by numeric rating scale (NRS) and the quality of life (QOL) was assessed by functional assessment of cancer therapy- general (FACT- G) scale. The clinical effect was evaluated according to RECIST criteria for solid tumor, and the patient’s survival time was recorded. Results (1) Twenty- six patients had local pain before the treatment, and the pain was relieved in different degrees after cryotherapy. The QOL, including all respects of FACT - G, was significantly improved after cryotherapy in all 61 patients. (2) The remission rate of the study group and the control group was 34.4% and 15.4% respectively, the difference between the two groups was statistically significant (P <0.05). (3) The median survival time of the study group and the control group was 12.9 months and 9.5 months respectively, and the one- year survival rate of the study group and the control group was 53.6% and 35.4% respectively. The differences between the two groups were statistically significant (P < 0.01). Conclusion Argon- helium knife cryotherapy is a safe and effective local treatment for advanced NSCLC, which can quickly reduce the tumor load, relieve the pain and improve patient’s quality of life. Cryotherapy with subsequent chemotherapy is superior to simple chemotherapy in improving the patient’s survival rate.

One hundred patients were diagnosed with coronary heart disease through dual-source CT coronary angiography (DSCTA) in our hospital,including 53 cases aged ≥60 y (elderly group) and 47 cases ＜60 y (middle-aged group).The curved-planar reconstruction (CPR),maximal intensity projection (MIP) and other post-processing reconstruction techniques were used to evaluate the indicators and differences of coronary stenosis ≥ 50％ ; the X-ray coronary angiography (CAG) findings were regarded as the gold standard.The evaluable rates of coronary arteries through DSCTA were 97.0％ and 98.6％ for two groups (P ＞0.05).The positive rate of coronary artery stenosis ≥ 50％ in elderly group was higher than that of middle-age group ( 12.6％ vs 9.0％ P ＜0.05 ).The rates of multi-vessel disease in elderly group (28.3％ ) was also significantly higher than that of middle-age group (6.4％,P ＜0.05 ).The sensitivity,specificity,positive predictive value and negative predictive value in evaluation of coronary artery stenosis ≥ 50％ for elderly patients were 92.3％,98.0％,88.0％ and 98.9％ respectively; those for middle-aged patients were 96.0％,99.0％,90.6％ and 99.6％ respectively ( P ＞0.05 ).DSCTA imaging provides an effective screening and evaluation method for coronary heart disease in elderly and middle-aged patients.

Objective To investigate the effect of di-( 2-ethylhexyl ) phthalate (DEHP) on pregnant rat and the protection of zinc.Methods Fifty rats were randomized equally into 5 groups consisting of blank (given 1 ml 0.9％ sodium chloride),corn oil (given 1 ml corn oil),zinc (given 1 ml zinc gluconate including 1.2 mg zinc),DEHP (given 50 mg · kg-1 · d-1 DEHP)and DEHP + zinc (given 50 mg· kg-1 · d-1 DEHP + zinc gluconate ).At the beginning of the experiment (about 7 d before pregnancy),the female rats were administered with corresponding drugs everyday.The pregnant rats were killed and the fetal rats were removed on 19th day.The following results in each group were recorded:the body weight and the organic weight of the female rats,the number and the weight of fetus rats and the placental weight.Results The weight and the coefficient of female rats' kidney/body,spleen/body,brain/ body,and heart/body in DEHP group compared with other groups were not statistical significance (all P ＞0.05).The coefficient of female rats' liver/body,uterus/body,and ovary/body of blank group were (4.4 ± 0.7 ) ％,( 1.26 ± 0.09 ) ％,( 0.083 ± 0.009 ) ％ respectively,corn oil group were (4.5 ± 0.6 ) ％,( 1.29 ±0.10)％,(0.084 ±0.008)％,zinc group were (4.4 ±0.4)％,( 1.26 ±0.08)％,(0.084 ± 0.009 ) ％,DEHP group were ( 5.4 ± 1.0 ) ％,( 1.11 ± 0.08 ) ％,( 0.074 ± 0.012 ) ％,and DEHP + zinc group were (4.4 ± 1.0)％,( 1.28 ± 0.10)％,(0.082 ± 0.007)％ ; in DEHP group the coefficient of female rats' liver/body,uterus/body,and ovary/body compared with other groups was statistical significance ( P ＜ 0.05 ).The fetal quantity,fetal weight and placental weight of female rats of blank group were 12.8 ± 2.7,(6.03 ±0.16) g,( 1.00 ±0.03) g respectively,corn oil group were 13.6 ±3.1,(6.07 ±0.20) g,(1.00±0.04) g,zinc group were 13.3 ±3.1,(6.16 ±0.18) g,( 1.00 ±0.05) g,DEHP group were 9.2±4.1,(4.03 ±0.09) g,(0.95 ±0.03) g,and zinc +DEHP group were 12.1 ±2.9,(6.09 ± 0.17 ) g,(0.99 ±0.03 ) g.In DEHP group the fetal quantity,fetal weight and placental weight of female rats compared with other groups were statistical significance ( P ＜ 0.05 ).Conclusion DEHP can damage female rats and fetal rats in gestation period.Zinc supplied before pregnancy can relieve the influence by DEHP.

Objective To investigate the impact of enteral nutrition (EN) through jejunal feeding tube on the nutritional status and immunologic function of patients during chemotherapy after total gastrectomy.Methods Totally 86 patients with gastric cancer who had undergone total gastrectomy with radical resection and jejunostomy received adjuvant venous chemotherapy in our department from January 2009 to June 2010.They were divided into EN group ( n =43 ) and control group ( n =43 ) according to the hospitalization number.The EN group maintained the jejunostomy tube until the end of 6 courses of chemotherapy,and was given EN support for 7 days through jejunal tube during each course of chemotherapy; the control group removed the tube before chemotherapy without further dietary restriction or nutrition support.The nutritional and immunologic indicators on the 1 st day before chemotherapy and the 1st day after 6 chemotherapy courses were measured.ResultsThree patients in the EN group withdrew from the study because of catheter blockage or catheter loss and 2 patients in the control group failed to complete the trial because of chemotherapy side effects or economic reasons; 81 patients entered the final analysis.After 6 courses of chemotherapy,both groups experienced body weight loss,but the percentage is significantly lower in EN group than in control group (6.9％ ± 0.95％ vs.11.2％ ± 1.32％,P =0.0000).Compared with the nutritional status before the first chemotherapy,the prognosis nutritional index (PNI) in both groups declined after the 6th chemotherapy.Such decline was not statistically significant in EN group ( P =0.1534) but was significant in control group (P =0.0000).The PNI in EN group after chemotherapy was significantly higher than that in control group (P =0.0040).The levels of IgG,NK,CD4 +,and CD4 +/CD8 + were significantly higher in EN group than in control group ( P =0.0083,0.0143,0.0000,and 0.0000,respectively) after chemotherapy.ConclusionEN during postoperative chemotherapy may improve the nutritional status and immunologic function in gastric cancer patients after total gastrectomy.