Objective:To investigate the feasibility and efficacy of focused ultrasound surgical clamp(FUSC)for partial hepatectomy,and find out the enhancement effect of SonoVue to FUSC.Methods:Twelve healthy goats were randomly divided into a FUSC group(n=6)in which FUSC was only used for the liver ablation,and a SonoVue + FUSC group(n=6)in which FUSC was performed immediately after iv injection of ultrasound contrast agent SonoVue.A FUSC was comprised of two ultrasound transducers fixed on a forceps-like handle.The directions of focused ultrasound beams produced by both transducers were opposite,with the same central axes.Ultrasound energy was continuously delivered by two transducers to ablate liver tissue located between both transducers.The frequency was 1.6 MHz,and the acoustic power was 150 W.All goats in both groups underwent laparotomy,and then the liver was totally exposed for an ablative procedure.Blood loss,FUSC exposure time,operation time required for the hepatectomy and the cross-section area of the resected liver were recorded.Histological changes were observed in the removed liver,and 10ml blood samples were collected in each goat to measure blood cells and hepatic functions after surgical procedure.All goats were followed up for complications and survival.Results:After surgical procedure,all animals survived well,and no complications were observed during follow-up period.Mean blood loss and mean exposure time were(16.9?4.2)ml and(174?9)s in the FUSC group,and(16.3?3.5)ml and(122?9)s in the SonoVue+FUSC group respectively.A statistical difference of average FUSC exposure time per unit area(P0.05)in both groups.Pathological examination showed coagulation necrosis of the targeted liver tissue,and small blood vessels were severely destroyed,with the occlusion of the ablated vessels.There was a transient increase of serum ALT,AST,TB and DB levels postoperatively in all goats,and they reduced to a normal level 7 days after surgical procedure.Conclusion:FUSC is feasible and effective for partial hepatectomy,which has advantage of convenience,less time and blood loss.FUSC combined with microbubble contrast agen(tSonoVue)can enhance the biological effects of FUSC and improve the efficiency of FUSC.

AIM To investigate the effects of levo phenoprolamine hydrochloride [ levo 1 (2,6 dimethylphenoxy) 2 (3,4 dimethoxyphenyl ethylamino) propane hydrochloride] [ l DDPH([?]D 25 1 08)]on experimental arrhythmias. on experimental arrhythmias. METHODS Intravenous administration of ouabain, aconitine or CaCl 2 induced arrhythmias in rats or guinea pigs; Microelectrode recording was used to record action potential; Whole cell patch clamp technique was used to record L type calcium current ( I Ca,L ). RESULTS ① l DDPH 50 mg?kg -1 inhibited the ventricular arrhythmias induced by intravenous injection of ouabain in guinea pigs or aconitine and CaCl 2 in rats. ② l DDPH 30 ?mol?L -1 shortened 50% action potential duration (APD 50 ) and prolonged effective refractory period (ERP) ( n =6, P

To discuss the availability of evaluation on the dissolution studies of the multicomponents in traditional Chinese medicine, the in vitro dissolution of total composition of the tablet of rhizomes of Ligusticum chuanxiong components and its correlation with the in vivo were studied by the method of area under the absorbance-wavelength curve (AUAWC). Taken the tablet of rhizomes of Ligusticum chuanxiong components which is composed of sodium ferulate and ligustrazine hydrochloride as subject model, the dissolution tests were carried out with basket method. The plasma concentrations of tablets in different rats were determined by AUAWC at different interval times. The in vivo absorption percentage was calculated by Wagner-Nelson equation to evaluate the in vitro and in vivo correlation. According to the results, the cumulative dissolution in vitro of total composition of tablets of rhizomes of Ligusticum chuanxiong components at 60 min was 90.65% in water by AUAWC. The in vivo pharmacokinetics is fitted with an one-compartment model. The linear equation based on the cumulative dissolution rate (fr) and absorption percentage (fa) at 5, 10, 20, 30 and 60 min was fa = 0.819 7 fr+0.183 and the correlation coefficient was 0.959 5, which showed a good correlation between the in vitro dissolution and the in vivo absorption percentage. The method of AUAWC can be used accurately, feasibly and conveniently to evaluate the in vitro and in vivo correlation of total composition of tablets of rhizomes of Ligusticum chuanxiong components, which will provide better guidance to study the in vitro and in vivo correlation of sustained release preparation etc under complex system of traditional Chinese medicine in the future.

Based on LPL model and clinical data collected, the correlation of ?/? factor with tissue volume irradiated has been derived. The data calculated by the new formula are coincidental with the results of clinical and animal experiments.

Objective: To compare the quality of life before and after artificial mechanical heart valve replacement. Methods: From February to June 1998, 115 patients (56 males, 59 females) underwent mechanical valve replacement. The investigation table was filled in one week before the operation and three months postoperatively. Results: There was a notable improvement in patient's physical strength, activity ability, emotion aspect and society home life after operation (P=0.000). In patients with valve-related complications, 64% complained inconvenience in purchasing anticoagulant medicines in the locality, while in patients without valve-related complications, only 39% have such experience. There was significant difference between two groups (P=0.034). 86% of patients with complications worry about the possibility bleeding, while in those with no complications only 55% (P=0.006). Conclusion: Mechanical prosthetic valve replacement significantly improves patient's physical strength, activity ability, emotion and society-home life.

Objective To experimentally verify the putative function of gene tls from Pseudomonas aeruginosa phage PaP3. Methods The gene tls was amplified from the genome of phage PaP3 by PCR and subcloned into pMD18-T vector. Then the gene tls cut down from the vector was inserted into the plasmid pQE31, which could give the 6-His tag at the N' side of the expressed protein. The recombinant vector pQE-tls was transformed to E. coli JM109. After induction with IPTG, the expressed bacteria was resuspended and sonicated, then the inclusion body was obtained after centrifugalization. The inclusion body was then dissolved with lysis buffer. The inclusion body solution, which has the target protein, was then purified by affinity chromatography and renatured by dialysis. Finally the nuclease activity of the fusion protein H6-TLS was tested on the plasmid pMD-cos which contains the cutting site of terminase large subunit. Results The expression plasmid pQE31-tls was successfully constructed, and the target protein yield was up to 30% of the total bacterial proteins. Meanwhile the substrate vector pMD-cos was also successfully constructed. After purification and renaturation, the fusion protein H6-TLS could partially cut the substrate vector pMD-cos. Conclusion The fusion protein H6-TLS was successfully expressed, purified and renatured, and it was shown to possess nuclease function. The experiment lays the foundation for further research of the gene tls.

Objective To develop LabVIEW-based digital PCG analyzer. Methods A kind of Digital PCG analyzer based on LabVIEW was designed. It included a heart sound sensor, an analog filtering circuit and amplifying circuit, an AD converter, a digital data transferring model and a software for PCG analyzing. Meanwhile, a kind of 3-lead ECG sampling circuit and a display model in LabVIEW were designed as a reference signal for PCG. distinction. Results This digital PCG analyzer had the functions of heart sound sampling from body surface, PCG real-time displaying on the software, spectrum analyzing for the PCG data and PCG database managing. Conclusion This kind of digital PCG analyzer based on LabVIEW can be used for assistant diagnosis of cardiac Arrhythmia and cardiac abnormal noise, and as a teaching tool for clinical auscultation.

Objective To discuss the feasibility of stereotactic radiotherapy plan designing based on MRI. Methods Based on the study of the serious geometric distortion of MRI while MRI was used in the condition of position by the experiment, how to deal with geometric distortion of MRI for patient positioning by the means of software was studied deeply. The basic thought of correction arithmetic for patient positioning images, of which the corrected data being used to correct patient images was created by primitive sparse corrected data calculated from design data and sampling data was brought forward. Supported by arithmetic research, the MRIC software system was designed and implemented. At last, the experiment model MRI was dealt with the MRIC system. Results The correction arithmetic was proved valid by the result of the experiment model MRI can be corrected back to normal state with MRIC system, which implemented the correction arithmetic. Conclusion It's possible that MRI would be used for positioning and plan designing in SRT.

Objective To analyze the clinical characteristics of primary retroperitoneal tumors(PRPT) and to improve the diagnosis and treatment of these tumors.Methods The clinical characteristics, surgical treatment and prognosis in 352 cases of primary retroperitoneal tumors were retrospectively studied, and the factors affecting postoperative recurrence were also investigated. Results The most common clinical manifestations in 352 cases of PRPT included abdominal mass,abdominal pain,abdominal distention, and lumbar pain. The size of the majority of PRPTs was large, and pathological patterns of them were varied. Urinary system, digestive system and large vessels were often involved. The resection rate was 87.99%. Removal of the involving organs or vessels were often necessary to achieve a complete resection of the tumor. The nature of the tumor and completeness of tumor were the major risk factors for postoperative recurrence. Conclusion The clinical manifestations, pathological types, involved organs and surgical treatment are complicated in PRPTs. Complete resection is the only effective method for the treatment of PRPTs, and it is one of the major factors in reducing recurrence.

Objective To evaluate the value of the use of fibrin glue in radical mastectorny for breast cancer. Method 52 patients with breast cancer were divided randomly into two groups. In one group (27 patients) fibrin glue was used during radical mastectomy, while in the other group (25 patients), fibrin glue was not used. The volume of drainage on the first postoperative day and total volume of drainage after the operation were collected and measured. The fluid accumulation underneath the flap was also observed. Result In patients with the use of fibrin glue, the amount of drainage was much less compared with those without the use of fibrin glue. Having marked diversity ( P