Pyroptosis is a new way of programmed cell death,a number of researches have found that it is associated with a variety of diseases.Inflammasome and caspase protein family play key roles in regulating pyroptosis.Intracellular pattern recognition receptor oligomers under external stimulus,then assemble with apop-totic speck-like protein containing a caspase recruitment domain and caspase precursor into inflammatory com-plex body,thus activation of caspase can induce pyroptosis.While as the upstream regulation mechanism of pyroptosis,inflammasome might be double edged swordfor tumor growth.On the one hand,inflammasome can inhibit the proliferation of tumor cells by inducing pyroptosis;on the other hand,the cumulative effect of the inflammsome can also form a suitable microenvironment for tumor cells and promote tumor growth.

OBJECTIVE: To observe the influence of Xiaotan Sanjie Recipe on growth of the transplanted tumor and expressions of proliferating cell nuclear antigen (PCNA) and epidermal growth factor receptor (EGFR) in tissue of the gastric carcinoma of nude mice, and to explore the mechanism of Xiaotan Sanjie Recipe in restraining the multiplication of the stomach cancer. METHODS: MKN-45 gastric carcinoma model in nude mice was established. Forty-five nude mice were randomly divided into control group, Xiaotan Sanjie group and 5-fluorouracil (5-FU) group. Drugs were given on the next day of inoculation. Mice in the control group were given normal saline, and mice in the Xiaotan Sanjie group were given Xiaotan Sanjie Recipe. Intraperitoneal injection of 5-FU was administered in the 5-FU group. The expressions of PCNA and EGFR were examined by using streptavidin peroxidase (SP) conjugate technique, and the tumor weight and pathological characteristics were also observed. RESULTS: Compared with the control group, Xiaotan Sanjie Recipe significantly restrained the growth of the transplanted tumor, and reduced the expressions of PCNA and EGFR in tissue of the gastric carcinoma of nude mice (P<0.05). CONCLUSION: Xiaotan Sanjie Recipe can disturb the synthesis of DNA and reduce the proliferous activity of cancer cells by decreasing the expressions of PCNA and EGFR.

Objective To observe the effects of health promotion on tumor recurrence,quality of life and self feeling evaluation of patients with superficial bladder carcinoma.Methods 104 patients with superficial bladder carcinoma treated in our hospital from March 2002 to March 2005 were divided to two groups randomly.The patients in one group were treated by bladder irrigation with Bacille Calmette Guerin (BCG)simply,and those in the other group were treated by bladder irrigation and health promotion.Tumor recurrence,quality of life and self feeling evaluation between the two groups were compared.Results All patients were foUowed up for 7.3-36.0 months(mean 28.5 months).17 patients(15/51)in the simple BCG irrigation group had tumor recurrence in 0-12 months(2 patients),13-24 months(6)and 25- 36months(9)respectively.The recurrence rate in the first year was 3.9%.in the second year 11.8% and in the third year 17.6%.Eleven(11/53)patients had in the BCG irrigation and health promotion group.in 0-12 months(2),13-24 months(7),and 25-36 months(2)respectively.The recurrence rate in the first year was 3.8%.in the second year 13.2%.and in the third year 3.8%.There were no significant differences in the recurrence rates of the first and second year between the two groups(P>0.05).but there was significant difieFence in the recurrence rate of the third year between the two group(Y2=5.29,P< 0.05).There were no significant difierences in self feeling SCL-90 evaluation and quality evaluation of life after the first discharging between the two groups(P>0.05).After 3-year follow up,the life quality of the patients in the BCG irrigation and health promotion group was improved obviously compared with the simple BCG group(X2=6.47,P<0.05),and the bad negative emotion was diminished obviously compared with the simple BCG group(P<0.05).Conclusion Health promotion after operation on the patients with superficial bladder carcinoma can decrease the long-term tumor recurrence rate obviously,and improve the quality of life and self feeling significantly.

[Objective]To investigate the capability of synthesis BMP-2-derived peptide on osteogenic induction in bone marrow stem cells(BMSEs),and to evaluate the osteoinductivity of BMP-2-derived peptide in vitro.[Method]After segregating and cultivating four weeks Wistar rats marrow stromal cells in induction group were induced by osteogenasis medium containing 200 gg/ml BMP-2-derived peptide,and in non-inductional group BMSCs were still culture with DMEM medium.Following continue culture for 2~4 weeks,cells film preparation,alkaline phosphatase activity and calcium deposition were measured.Type I collagen(CoM)and osteopontin(OPN)mRNA expression were measured using real-time fluorescent quantitative polymerase chain reaction(FQ-PCR)technique.The capability of synthesis BMP-2-derived peptide on osteogenic induction of BMSCs was investigated.[Result]After inductived with BMP-2-derived peptide.BMSCs cultured survived well greatly changed in cell morphology,and showed a biological and morphologie characteristics similar to those of osteoblasts.The lever of alkaline phosphatase activity and calcium deposition increased.Col-Ⅰand OPN mRNA were expressed at higher level.In non-inductional group no conspicuous osteogenic induction was shown.[Conclusion]BMP-2-derived peptide can induce BMSCs to differentiate into osteoblasts.It has the similar capacity of osteogenic induction as nature BMP-2,so BMP-2-derived peptide is an ideal cell agent for bone tissue engineering,and can be available widely.

[Objective]To evaluate the diagnostic strategy for bone metastasis from unknown primary. [Method]One hundred and twenty five patients with unknown primary bone metastasis were treated from June 2003 and December 2006.The distribution of bone metastasis while initial diagnosis mainly focused on spine,sacrum,pelvis,femur and humerus.The diagnostic strategy contained a series of physical examination,laboratory test,imaging study and pathological examination.[Result]The primary tumor was found in 70.4% cases,while 40% was in the lung,8% in kidney,5.6% in prostate,4.8% in liver,4% in breast,both 2.4% in thyroid and gastric,and 3.2% in other visceras.The physical examination revealed the occult primary site of the malignant tumor in only 9.6% cases.Although,43.2% patients had higher level of tumor antigen,but most of them were non-specific.Sixty percent of primary tumor was found by various imaging studies including plain radiograph,computed tomography and ultrasound.The pathological result after biopsy or operation identified or suggested the primary tumor in 66.4% cases.The one year survival rates after diagnosis were 58.57% and 22.93% in patients of bone metastasis with known and unknown origin,respectively.[Conclusion]The primary tumor can be diagnosed in most bone metastasis patients with no cancer history after careful examination,and patients will get benefit on survival time for targeted treatment.

Objective To explore the related factors for improving the effect of the staged bilateral multi-targets pallidotomy and thalamotomy on Parkinson's disease.Methods 387 patients were divided into different groups according to various factors,including the age of patient,operation method,interval of radiofrequency and the diameter of electrode. The relationship between the above mentioned factors and the effect or complication was analyzed.Results All the symptoms of PD were significantly improved after second operation in the near future. Staged bilateral pallidotomy was the most popular operation way in clinical practicing. The incidence of complication of staged bilateral thalamotomy was high (100%). The rate of complication in the patients over 55 years old (29.70%) was higher than that in the patients not more than 55 years old (12.66%)( P

Bluetooth is a new widely accepted standard of short distance communication in personal area networks (PANs). By analyzing the communication process based on Bluetooth protocol and requirements of physiological electrical signal transmission, such operations are performed as the design of Bluetooth model for measurement of heart sound, the development of Bluetooth network communication library and the implementation of the model by programming.

The general theories of gray correction methods for the medical ultrasonic image are introduced in this paper, including gray level correction, gray level transformation and histogram correction. The dominant histogram equalization method is expatiated and modified against its disadvantages including inapparent enhancement of partial detail and non-interactivity. Series connections of several gray correction methods are used to treat with the ultrasonic image, and thus its utility is improved through the selective enhancement of the contrast of certain gray scope.

Objective: To observate the clinical effect of Saffron Multi-glycoside Tablets for cardiac angina belonging to heart blood stasis type. Methods: Saffron Multi-glycoside Tablets were administered orally three times daily, four tablets each time. Results: Not only the degree of heart impediment and chest pain but also attack times and persistent pain changed for the better obviously. The effective rate is 96.67% and the effective rate of ECG is 93.33%, the amelioration rate of Chinese medical symptom is 96.67%. The control group is 72.22%, 66.67% and 69.44%, respectively. There is significant difference between the two groups. Conclusion: There is notable effect to take Saffron Multi-glycoside Tablets for cardiac angina belonging to heart blood stasis type.

Objective To investigate the value of the combined detection of serum human epididymal secretory protein 4(HE4) and carbohydrate antigen 125(CA125) in diagnosing ovarian and uterine tumor .Methods Serum levels of HE4 and CA125 were detected in 112 cases of ovarian cancer ,92 cases of uterine cancer ,145 cases of ovarian benign diseases ,138 cases of uterine adeno-myoma and 56 cases of healthy controls by ELISA and electrochemiluminescence(ECL) immunoassay respectively .Results Serum levels of HE4 and CA125 were (246 .80 ± 52 .81)pmol/L and (439 .20 ± 305 .80)U/L in the ovarian malignant tumor group , (196 .50 ± 38 .15)pmol/L and (463 .80 ± 127 .60) U/mL in the uterine malignant tumor group and (19 .76 ± 16 .45)pmol/L and (15 .67 ± 11 .19)U/mL in the control group ,the differences had statistical significance (P<0 .05) .Serum levels of HE4 and CA125 before operation and in postoperative 2 weeks in the ovarian cancer group and the uterine cancer group had statistical difference (P<0 .05) .Conclusion The combination detection of serum HE4 and CA125 can improve the accuracy and sensitivity in the diag-nosis of pelvic tumors .