Through intensifying the clinical pathological discussions and clinical pathological dissections,the clinical thinking,iatri- cal responsibility and analytical ability of the medical students have been improved markedly and the abridge function of pathology between basic medicine and clinical medicine has also been reinforced.

[Objective] To observe the clinical efficacy of ZICAOYOUSHA in treating diabetic foot ulcers.[Method] A multi-center ,randomized,double-blind,placebo-control ed study was conducted. A total of 232 patients with diabetic foot ulcers were randomly assigned the treatment group and control group,in foundation treatment at the same time,the therapy group which was treated by External Application ZICAOYOUSHA had 174 patients,the contrast group which was treated by External Application Gentamicin Emery cloth had 58 patients. Observe the aspect improvement situation in two groups separately in accordance with Wagner grading,carry out statistics processing.[Results] Two groups of curative effect indices had significant differ-ence. [Conclusion] ZICAOYOUSHA is an effective drug for external use in treating diabetic foot ulcers.

Objective To compare the analgesia and side-effects of different concentrations of sufentanil combined with 0.125% ropivacaine for postoperative epidural analgesia after general thoracic surgery.Methods Thirty-six ASA Ⅰ-Ⅲ patients (25 males, 11 females) ages 21-64 yrs weighing 42-79 kg undergoing elective general thoracic surgery under general anesthesia were randomly assigned to receive patient-controlled epidural analgesia (PCEA) with 0.125% ropivacaine combined with sufentanil 0.4 (group A, n = 12) , 0.5 (group B, n = 12) or 0.6 ?g?ml-1 (group C, n = 12) . Epidural catheter was placed at T7,8 or T8,9 interspace. The PCEA pump was set up with back ground infusion of 2 ml?h-1 , a3ml bolus dose and a 30-min lock-out period. VAS scores was used to assess analgesia at rest and during movement. The total bolus doses, PCEA button pressing times (effective/actual), vital signs including MAP, HR, respiratory rate, SpO2 and side effects (nausea, vomiting, pruritus and dyspnea) were recorded. Results During the 48 hours after operation the VAS scores in group C were significantly lower than those in group A and B ( P

Objective To investigate the diagnostic value of MRI in patients with ischiofemoral impingement syndrome (IFIS). Methods MRI data of 70 patients with IFIS (IFIS group) and 40 normal volunteers (control group) were analyzed retrospectively. The width of ischial femoral space (IFS) and quadratus femoris space (QFS) were measured on axial fat suppression T2WI, while the angle of sciatic bone was measured on axial T1WI, and the femoral neck shaft angle was measured on coronal T2WI, and then were compared between the two groups. The correlation between the width of IFS and the other three parameters was analyzed, and ROC curve was drawn to evaluate the diagnostic efficacy for IFIS. The degree of edema and fat infiltration of the quadratus femoris in IFIS group were evaluated, and the differences of IFS width among different grades were compared. Results In IFIS group, the IFS width, QFS width, ischium angle and femoral neck shaft angle was (11.76±2.22)mm, (8.33±2.20)mm, (132.59±1.39)° and 132.70(131.18,134.13)°, respectively, and the differences between the two groups were statistically significant (all P<0.001). The area under ROC curve in diagnosis of IFIS with IFS width, QFS width, ischium angle and femoral neck shaft angle was 1.000, 0.999, 0.996 and 0.975, respectively (all P<0.001). There was positive correlation between IFS width and QFS (r=0.743, P<0.001), negative correlation between IFS width and ischium angle and femoral neck shaft angle (r=-0.273, P=0.022; r=-0.332, P=0.005). The overall differences in IFS width among different grades of femoral quadratus edema and fat infiltration in IFIS patients were statistically significant (both P<0.05). Conclusion IFS and QFS of IFIS patients are obviously narrow. Edema and fat infiltration of quadratus femoris are common MRI findings in IFIS patients.

Aim To study the anti-inflammatory activity of the Channa argus bile.Methods The bile was isolated and purified by extraction and silica gel column chromatography.Then the compounds were identified by hydrogen and carbon spectra.The spleen lymphocytes proliferation assay and Lipopolysaccharide(LPS) induced mouse macrophage RAW264.7 releasing Nitrogen Monoxide(NO) experiment were used to evaluate the anti-inflammatory activity.Results Compound(C1) of sodium taurocholate and compound(C2) of sodium taurochenodeoxycholate were isolated by activity tracing.The cell relative viabilities of the two compounds on Concanavalin A(Con A) induced spleen lymphocytes proliferation assay were 65.9%±11.7% and 60.5%±9.4%, which were significantly different from the result of model group (P<0.01), respectively.The NO production of LPS-induced RAW264.7 release of NO was (16.4±1.9) μmol·L-1 and (15.5±1.7) μmol·L-1, which were significantly different from the result of model group(P<0.01).Conclusion Sodium taurocholate and sodium taurochenodeoxycholate from Channa argus perform the anti-inflammatory activities but have no cytotoxic effect on spleen lymphocytes and macrophage.

Objective:To investigate the antitumor effect of Actinidia Chinensis Planch polysaccharide(ACPS)in B16-bearing C57BL/6 mice and approach its mechanism.Methods:The C57BL/6 mice model was established by B16 subcutaneous inoculation,giving the polysaccharides from vena caudalis injection,and flow cytometer(FCM)was used to detect the distribution of tumor-cell cycle,and electron microscope was used to survey morphologic transformation about apoptosis.Results:ACPS can inhibit the growth of B16,the high,middle dose groups obviously restrained the tumor with the rate of 48.67%,40.90%and the control group 24.13%,and classical apoptosis corpuscles had been found through electron microscope in ACPS groups.Compared with control group,the ACPS promoted the spleen-index of B16-bearing mice and cut down the proliferation index,and the G1/S phase was at growth-inhibitory concentrations judged by the distributing analysis on cell-cycle.Conclusion:ACPS had obvious effect of restraining B16 and promoting the spleen index of tumor-bearing mice,which maybe due to its function of regulating immunization and the distributing of cell-cycle.

Objective To employ 2 approaches to construct and express reconstructed LL-37 (rLL-37) in procaryotic system, and to explore a better preparation method. Methods The first method: the rLL-37 was inserted into vector pET-28a (+), then was induced to express in E.coli. BL21 (DE3) and purified by chromatography; the second method: the rare codons in the rLL-37 gene sequence were substituted by the preferred codons of procaryotic cell, and a fragment of carrier protein molecule (CPM) was added to the N termination of the objective sequence to construct expression plasmid pET-30a(+)-CPM-rLL-37, then the rLL-37 was expressed in E.coli. BL21 Star(DE3) and purified by chromatography. The productive rates of the 2 methods were compared and the antimicrobial effects of obtained rLL-37 was studied. Results The first method: the DNA sequence of rLL-37 was obtained successively by Touch-Down PCR. The expression plasmid pET-30a(+)-CPM-rLL-37 was expressed with fusion protein in E.coli BL21 (DE3). The expression rate accounted for 20% of total bacterio-protein, then the expressed product was purified by using high positive ion exchange column Macro-Prep High S; The second method: a fragment of carrier protein molecule was designed that contained 28 amino-acid residue and its pHi was 2.7, net charge was-6.0 at pH 7.4. After the expression plasmid pET-30a(+)-CPM-rLL-37 was constructed successively, it was expressed in E.coli BL21 Star (DE3). The expressed fusion protein accounted for 35% of total bacterio-protein, then the expressed product was purified by using affinity binding chromatography with TALON resins successfully. The obtained 2 kinds of rLL-37 were able to kill both Gram-negative and-positive bacteria by the means of inhibitory zone. Conclusion It’s feasible to prepare efficiently rLL-37 in procaryotic system, which founds the basis for the further research on bactericidal activity of rLL-37.

Objective To introduce the mutated gene coding cysteine into the gene of dsFv VL of human antibody to N terminal fragment of lipopolysaccharide binding protein(LBP)and to express,purify the mutated dsFv VL in bacterium.Methods We reconstructed and sequenced the mutated gene of VL of human mAb Fab to LBP by Mega-primer PCR based on point mutagenesis method.Some codes of FWR1 of VL had been replaced by TGT in order to code cysteine.The DNA sequence of reconstructed VL was inserted into vector pET-28a(+),then VL was expressed by E.coli.BL21 star(DE3)and was purified by chromatography.Finally the activity of VL to bind NH-LBP was determined by ELISA.Results The results showed that the cysteine was introduced into the position 21 amino acid of VL to replace the threonine.The gene of VL was about 650 bp and relative molecular weight of VL was 28?103.VL could bind NH-LBP directly.Conclusion These have laid a foundation for producing the dsFv against NH-LBP.

Objective To construct the recombinant adenovirus vector containing human survivin, and transfect it into dendritic cells. Methods Full length survivin cDNA was obtained from recombinant plasmid pCITE-survivin by PCR. The PCR product was double-digested with restriction endonucleases KpnⅠ and XholⅠ, and inserted orientationally into pAdTrack-CMV. The plasmid of pAdTrack-survivin was lined with PmeⅠ, and the fragment containing survivin was reclaimed and transfected into E. coli. BJ5183. After having been screened, the extracted plasmid of positive bacteria was transfected into HEK293 cells with liposome and was identified by the green fluorescence protein (GFP) expression and by PCR method. The virus was transfected into dentritic cells, and the expression of survivin was proved by the GFP expression and Western blot analysis. Results The recombined adenovirus-survivin was constructed successfully and the titer was about 1.65?10 8 pfu/ml. A band was observed by Western boltting and its relative molecular mass was about 16.5?10 3. Conclusion A recombinant adenovirus vector containing human survivin was constructed successfully, and the survivin protein was expressed by dendritic cells.