Objective To explore the effect of electroacupuncture combined with personalized pleasure factor on the rehabilitation of patients with poststroke depression.Methods 120 patients with poststroke depression were selected and randomly divided into two groups.60 patients in the observation group were given electroacupuncture plus individualized pleasure factor intervention,while 60 patients in the control group were given electroacupuncture only.The duration of treatment in both two groups was 3 months.The self-rating depression scale(SDS) of the two groups was compared before and after treatment.The quality of life before and after the intervention was assessed by the SF-36 health questionnaire.The limb function evaluation criteria were used to evaluate the linb motor function.The ability for living was assessed by activity of daily living scale(ADL).The cognitive function of the patients before and after the treatment was evaluated by the MMSE (Simple Mental State Examination).Results After treatment,the SDS score of the observation group was significantly lower than that of the control group (t =3.043,P =0.034).After 6 months of treatment,the overall health of the observation group was significantly better than that of the control group (t =3.236,P =0.032),the cognitive function of the observation group was significantly better than that of the control group(t =3.133,P =0.036).In the observation group,the fully independent ratio of ADL of the observation group was significantly higher than that of the control group,the difference was statistically significant (t =14.252,P =0.000).Conclusion It has great clinical significance to improve the daily living ability,limb function and cognitive function of patients with poststroke depression by electroacupuncture combined with individualized pleasure factor input,and improve the patients'depression and quality of life.

Objective To explore types and risk factors of recurrent ischemic stroke in ischemic stroke patients using antiplatelet drugs. Methods By retrospective study, 130 recurrent ischemic stroke patients using antiplatelet drugs (antiplatelet therapy group) and 101 recurrent ischemic stroke patients not taking antiplatelet drugs (non antiplatelet therapy group) were collected. Two groups of patients were divided according to the ischemic subtypes, and risk factors between different subtypes were analyzed. Results (1) Single factor analysis showed history of coronary heart disease and smoking are more frequent in antiplatelet therapy group with recurrent lacunar infarction (LI). History of coronary heart disease, diabetes, and high level of low density lipoprotein (LDL) are more frequent in antiplatelet therapy group suffer from recurrent atherosclerotic thrombosis (AT). (2)Logistic regression analysis showed the history of coronary heart disease , smoking are independent risk factors for recurrent LI in antiplatelet therapy group (P = 0.018, P = 0.027); history of coronary heart disease, diabetes, and high level of LDL are independent risk factors for recurrent AT in antiplatelet therapy group (P =0.003, P = 0.010, P = 0.002). Conclusions The history of coronary heart disease and smoking are independent risk factors for the recurrence of LI in patients with antiplatelet therapy. History of coronary heart disease , diabetes , and high level of LDL are independent risk factors for the recurrence of AT in patients with antiplatelet therapy. Combined coronary heart disease would increase both LI and AT recurrence.

Objective: To establish a method for the quality standard of Biyankang granule. Methods: Scutellaria baicalensis Georgi and Puerariae Iobatae radix were identified by TLC. The content of baicalin was determined by HPLC with a Dubhe C18 column (250 mm × 4. 6 mm, 5 mm). The mobile phase was methanol and 0. 4% phosphoric acid with gradient elution. The flow rate was 1. 0 ml· min-1 and the detection wavelength was 280 nm. The column temperature was 30℃ and the injection volume was 10 ml. Re-sults:The spots in TLC were clear without any interference. A good linear relationship was shown within the range of 15. 02-300. 43 m g·ml-1 for baicalin (r=1.000 0). The average recovery of baicalin was 102.62% (RSD =0.72%, n =6). Conclusion: The method is specific with high accuracy and good reproducibility, which can be used for the quality control of Biyankang granule.

AIM:To study the protectective effect of bacalin on myocardial ischemia in isolated rat heart and its mechanism.METHODS:Thirty six Sprague-Dawley rats were randomly divided into 3 groups.The heart of rats in control group(n=12)were perfused for 1 h and 50 min incessantly.The heart of rats in ischemia group(n=12)received 25 min of perfusion first and then subjected to 25 min of global ischemia followed by 1 h reperfusion.The heart of rats in baicalin group(n=12)received 5 min perfusion of 5 mg/L)baicalin before ischemia while other procession was the same as ischemia group.The hemodynamic measurement and the coronary flow were recorded.The levels of CK and LDH in the coronary effluent were detected.The expression of p38mapk,NF-?B and the levels of TNF-? and IL-6 in myocardium were respectively detected by immumohistochemical technique and ELISA.RESULT:The function of hearts treated with baicalin was improved significantly,and the level of LDH and CK of coronary effluent were decreased significantly compared with ischemia group.The level of TNF-? and IL-6 and the expression of p38mapk and NF-?B in myocardium also decreased observably compared with ischemia group.CONCLUSION:Baicalin has a protective effect on myocardial ischemia of isolated rat heart and the effect may be due to the decrease of TNF-? and IL-6 induced by the reduction of the expression of p38mapk and NF-?B.

Objective To analyse the X-ray characteristics of the intraductal papilloma and to improve the diagnostic accuracy.Methods The mammographic data of 80 cases of intraductal papilloma of breast by pathology were analysed.Results 24 cases showed circular filling defect, 12 cases showed stringy filling defect,ductal blocked in 30 cases, ductal dilation in 48 cases. Conclusion Galactography is a effective method for diagnosing intraductal papilloma of the breast.

Objective To observe the curative effects and explore the homeostasis mechanism of Fuxueningheji in treating metrorrhagia with blood stasis.Methods 60 patients were divided randomly into test group(30 cases,treated with Fuxueningheji)and control group(30 cases,treated with Gongxuening pulse),one course was 1 month.Results The total effective rate of treatment group was 93.33%,and markedly effective rate was 66.67%,while the control group was 66.67% and 40% respectively.There was no significant difference in total effective rate,but was significant difference in markedly effective rate.Fuxueningheji could improve clinical symptoms of patients obviously and reduced the bleeding and coagulation time,improve the hemorheology,decrease PGI2,increase TXB2.The effects was superior to that of the control group(P

Objective To evaluate the clinical manifestations and responses to treatment of adult onset Still's disease (AOSD).Methods One hundred and four AOSD patients were retrospectively analyzed with clinical features and treatment responses.Results The major clinical features were:spiking fever (100%),evanescent maculopapular rash (95%),polyarthralgia (90%),sore throat (78%),lymphadenectasis (66%),hepatosplenomegaly (57%),hydmhymenitis (30%),neutrephilia (98%),liver dysfunction (62%),increased erythrocyte sedimentation rate (96%) and hyperferritinaemia (99%).Reactive lymphnodes hyperplasia was shown in all patients underwent lymphnode biopsy.94% and 66% patients were treated with glucocorticoid and immune suppressants.Those treated with ≥40 mg/d prednisone or its equivalent dosage achieved quicker remission and less relapses.Conclusion Patients who present with spiking fever,polyarthralgia,maculopapular rash,sore throat and predominant neutrophilia should be considered the possibility of AOSD after tumor or infection are excluded.Glucoeorticoid and immune suppressive drugs are effective medications for AOSD.

OBJECTIVE:To establish a method for the simultaneous determination of ethambutol(EMB)and isoniazid(INH) in children’s plasma. METHODS:After precipitated with acetonitrile,plasma samples were determined by UPLC-MS/MS method. Using lamivudine as internal standard,Waters ACQUITY? UPLC HSS T3 column was adopted with mobile phase consisted of ace-tonitrile-water(containing 0.05% formic acid and 15 mmol/L ammonium formate)in gradient mode at the flow rate of 0.2 ml/min. By ESI,positive ion detection was conducted in MRM mode. The monitoring transition ion-pair was m/z 205.2→116.0 for EMB, m/z 138.1→121.1 for INH and m/z 230.2→111.9 for internal standard. RESULTS:The linear range of EMB and INH was 10-5 000 ng/ml and 50-5 000 ng/ml,respectively;the limits of quantitation were 10 and 50 ng/ml;RSDs of inter-day and intra-day were all lower than 15% and relative recoveries were 98.7%-105.5%. Plasma concentrations of EMB and INH in 4 children with tuberculo-sis were 94.5-99.7 and 55.1-80.9 ng/ml. CONCLUSIONS:The method is rapid and sensitive,which suitable for children’s plasma concentration monitoring of EMB and INH and pharmacokinetic study.

In recent years,there is an increasing focus on three Dimensional (3D) Technology in medical field.The article summarizes the progress of 3D printing technology such as medical models,implantable prosthetics,biological engineering,est.,analysis application and effects of 3D technology abiding by the characteristics of clinical medical education.Considering the situation of domestic medical education,therefore,it points out that it is of broad application prospects and great practical significance to develop 3D medical education due to establishing a harmonious teaching environment,providing simulated clinical practice opportunities,activating the brain proportionately and stimulating students learning enthusiasm.

Objective To discuss the value of choledochoscopy via subcutaneous jejunal blind loop in the diagnosis and treatment of severe complications after pancreatoduodenectomy. Methods A fiber choledochoscope (FCC) was introduced through a subcutaneous jejunal blind loop. Under direct vision, the pancreatojejunal or choledochojejunal anastomotic leakages were coated with biological glue, or the anastomotic bleedings were stopped with hemostatic drugs. Results Five cases of intestinal leakage, 3 cases of biliary leakage and 2 cases of anastomotic bleeding were all cured. Follow-up for 1～3 years in the 10 cases found no recurrence of tumors. Choledochojejunal anastomotic stricture occurred in 1 case 1 year after the surgery and was cured by dilatation under fiber choledochoscope. In the remaining 9 cases, the pancreatic juice and bile fluid were excreted normally. Conclusions Application of FCC through the subcutaneous jejunal blind loop can be employed in the diagnosis and treatment of complications after pancreatoduodenectomy and in the long-term follow-up of pancreatojejunal or choledochojejunal anastomotic stoma under direct vision.