Objective To study the changes of serum IFN -gamma,TNF alpha and IL -10 levels in ulcera-tive colitis and its relevance to helicobacter pylori infection.Methods 80 UC patients were selected as the research object,and 50 cases of 80 patients were in the activity stage of UC,30 patients were in remission.In the 50 patients with active UC:14 cases were mild,26 cases were moderate,and 10 cases were severe.30 healthy subjects were set as healthy controls.Detection of serum IFN -gamma,TNF alpha,IL -10 levels and Hp infection were made.Results The serum TNF alpha and IL -10 levels of the activity group were higher than those of the remission group and the control group,the difference was statistically significant[TNF alpha :(54.75 ±5.92)ng/L vs.(23.69 ±4.15)ng/L vs.(20.45 ±4.01)ng/L,F =36.79,P =0.000;IL -10 :(35.16 ±6.72)ng/L vs.(25.12 ±5.37)ng/L vs.(23.96 ± 5.21)ng/L,F =35.68,P =0.000],the serum IFN -gamma level of the activity group was lower than those of the remission group and the control group,the difference were statistically significant[(14.98 ±3.15)ng/L vs.(26.06 ± 4.31)ng/L vs.(27.16 ±4.39)ng/L,F =35.68,P =0.000].As the aggravating of patients with UC in activity stage,TNF alpha and IL -10 levels showed a trend of increase(F =32.81,24.95,all P <0.05),while IFN -gamma showed a trend of reduce(F =22.29,P <0.05).In 50 cases of active UC patients,18 patients with Hp positive, 32 cases were Hp negative.In active UC group Hp positive patients'serum TNF alpha and IL -10 levels higher than the Hp negative patients,the difference was statistically significant(t =4.62,4.35,all P <0.01).Serum levels of IFN-gamma of active UC patients with Hp positive group was lower than the Hp negative patients,the difference was sta-tistically significant(t =5.91,P <0.01).Conclusion In active UC patients expression of TNF alpha and IL -10 are high,and IFN -gamma expression is low.The expression of IFN -gamma,TNF alpha and IL -10 are closely related to Hp infection.

Objective: To study the effect of zinc deficiency(ZD)on the gene expression of vitamin D receptor(VDR)and CaBP in duodenal mucosa of rats. Methods: Thirty weaning male rats were randomly divided into three groups:ZD,paired-fed(PF),zinc adaquate(ZA). After 15 d feeding, took the duodenal mucosa to extract RNA,and measured the levels of VDR mRNA and CaBP mRNA in duodenal mucosa by real time fluorescent quantity PCR. Results: The VDR gene expression of ZD group was downregulated 88.89% as that of PF group,and 50.00% as that of ZA group. CaBP gene expression of ZD group was downregulated 80.16% as that of the PF group,and 88.50% as that of ZA group. Conclusion: Zinc deficiency, by changing the activity of VDR and the mRNA expression of VDR,affects the transcription of the target gene CaBP,and then the absorption of calcium that causes allo-osteogenesis.

Objective To investigate the feasibility of implementing the 24 hours treatment and management model of children with cerebral palsy in Shanghai,in order to provide a set of effective and saving manpower,material and financial rehabilitation and management model for children with cerebral palsy.Methods Firstly,the 10 exports engaged in cerebral palsy rehabilitation were selected as the in depth interviewees by the sampling method of grounded theory.Secondly,applying in depth interviews,the 10 exports were interviewed by designing interview outline and subjects.Last,the interview data were collected and analyzed.Results By analyzing the interview data in three-stage coding mode,three factors affecting the feasibility of 24 hours treatment and management model of children with cerebral palsy in Shanghai were obtained,including the favorable factors,obstacles and necessary factors.Conclusions The 24-hour treatment and management model of children with cerebral palsy in Shanghai is feasible,but it will encounter some obstacles in the implementation process.Through the policy support for health care and education sectors,the feasibility of the pattern will be greatly enhanced.

Objective To evaluate the effectiveness of scanning with labeled autologous leukocytes for the detection of abdominal inflammation in equivocal patients. Methods From July 1998 to April 2000 sixteen patients with equivocal abdominal inflammation were recruited into this study. ResultsThere were 10 cases with positive scanning. The diagnosis of intraabdominal infection was consequently confirned by laparotomy and or good response to antibiotic therapy.The 6 cases with negative result were eventualy proven to have no intraabdominal infection. The average radioactivity of imaging and paired non imaging district was determined ( P

In this paper, 6 kinds of SCI-indexed international rehabilitation journals and all the articles published in 3 kinds of China Rehabilitation Source Journals in recent 1 year were counted. This article compared the difference of research methods and topics of the direction between our country and foreign countries were compared, analyzed the basic principles of rehabilitation medicine and influencing factors about the topics, and the future trends in rehabilitation research.

Objective To evaluate the efficacy and safety of infliximab and traditional disease modifying antirheumatic drugs (DMARDs) in the treatment of ankylosing spondylitis (AS). Methods Sixty patients with definite AS were treated with infliximab 5 mg/kg infusion at 0, 2, 6, 12 weeks and were followed up for 12 weeks. The primary endpoint was proportion of ASAS 20 responders at week 12. The secondary endpoints were the proportion of ASAS 50, the change from baseline in Bath AS functional index (BASFI).The improvement of signs and symptoms of AS and physical function were evaluated. The statistical treatments were used t-test andA2 test. Results The proportion of ASAS 20 responders at 2, 6, 12 week was 70%, 83% and 93% respectively. The proportion of ASAS50 responders at patients at 2, 6, 12 week was 13%, 37% and 57% respectively. Results for other secondary efficacy endpoints showed that infliximab could provide substantial benefits to patients with AS by reducing clinical signs and symptoms and improving range of motion, physical function and quality of life. Ten percent of the subjects reported treatment- related adverse events. The most frequently occurred were upper respiratory tract infection, followed by gastrointestinal adverse events and infusion reaction. Most treatment-related adverse events were mild to moderate in severity and disappeared after drug withdrawal. Conclusion Infliximab has been demonstrated to be effective and is well tolerated in the treatment of AS.

Objective To evaluate the cytotoxicity of trichostatin A (TSA),a histone deacetylase inhibitor,plus paclitaxel to H1299 strain lung cancer cells. Methods H1299 cells were exposed to TSA and/or paclitaxel in different concentrations in different ways. The proliferation rates were determined by MTT assay,the 50% inhibition concentration (IC50) of paclitaxel was calculated and the growth curve was plotted. The H1299 cells were then divided into 4 groups:control group (cells were normally cultivated for 36h),TAX group (cells were treated with 10nmol/L of paclitaxel for 24h),TSA group (cells were treated with 300nmol/L of TSA for 24h) and TF group (cells were exposed to TAX for 24h after being treated with TSA). Cell cycle and apoptosis were determined by flow cytometry. The morphological changes in nuclei as stained with Hoechst 33342 were observed by fluorescence microscopy. The protein expression levels of p21 and cleaved poly-ADP ribose polymerase (PARP) were determined by Western blotting. Results TSA significantly enhanced the inhibition of paclitaxel in lung cancer cell lines H1299. When combined with TSA,the IC50 of paclitaxel decreased significantly from 110.6?38.7nmol/L to 63.7?11.8nmol/L in H1299 cells (P0.05). Conclusion The HDAC inhibitor TSA,combined with TAX,may enhance the cytotoxicity of paclitaxel to,and promote the death of,lung cancer cell line H1299,which may not be related to apoptosis.

Background Studies showed that 577 nm subliminal micropulse photocoagulation for retinal diseases is more effective and much safer than 577 nm laser,but its mechanism and sensitive target cells are still unelucidated.Objective This study was to compare the histomorphology changes of rabbit retinas following 577 nm subliminal micropulse excitation and 577 nm laser for retinal photocoagulation and offer a conference for the clinical application of 577 nm subliminal micropulse excitation.Methods Twenty-six Chinese pigment rabbits were randomly divided into normal control group (2 rabbits),577 nm laser photocoagulation group (6 rabbits) and subliminal micropulse photocoagulation group (18 rabbits),in which the rabbits from subliminal micropulse photocoagulation group were subgrouped into 9％,12％ and 15％ subthreshold photocoagulation groups according to the different workload rates,and retinal photocoagulation was performed based on grouping.No treatment was carried out in the normal control group.Fundus camera and OCT were obtained after photocoagulation,and the rabbits were sacrificed to prepare the specimen of eyeball wall.Hematoxylin and eosin staining was employed to examine the histopathological change of choroid and retina under the light microscope.Results The retinal morphology was normal on the fundus color photography and OCT in the normal control group.Obscure of retinal structure and slight edema of retinal neuroepithelial layer with obscure of retinal pigment epithelium (RPE) was found respectively in the 9％ and 12％ subthreshold photocoagulation group,and serious edema of retinal neuroepithelial layer with local RPE elevation was visible in the 15％ subthreshold photocoagulation group.The laser spot was invisible in various subliminal micro pulse groups.However,retinal photocoagulation spots,multifocal elevation of retinal layer and serious detachment of retinal neuroepithelium were found in the 577 nm laser group.Retinal histopathological examination revealed that the photoreceptors,bipolar cells and retinal ganglion cell (RGC) layers were normal,while choroidal vessel deformation and bleeding were visible in the 9％ and 12％ subthreshold photocoagulation groups,and only photoreceptor membrane swelling was seen in the 15％ subthreshold photocoagulation group.However,retinal structure abnormality was displayed in 577 nm laser group,including the structural disorders of inner and outer nuclear layers and RGC layer as well as thinning of RPE layer.Conclusions 577 nm nicropulse laser under threshold is sensitive for RPE and choroid rather than retina neurepithelium,which can play a treating role and also does not damage retina seriously.However,577 nm laser photocoagulation results in retinal structure damage.

Objective To investigate the efficacy of acupuncture combined with doctor-nurse cooperation mode on stroke-associated pneumonia in patients with acute stroke. Methods 67 patients of acute stroke within 1 week in our hospital from March, 2013 to January, 2015 were randomly divided into control group (n=35) and treatment group (n=32). Both groups received neurological routine treatment and systematic rehabilitation training by professional physiotherapists under general nursing mode. The treatment group received acupuncture treatment under doctor-nurse cooperation mode additionally. Incidence of stroke-associated pneumonia, swallow function and aspiration rate were recorded. Results Compared with the control group, the incidence of stroke-associated pneumonia significantly decreased in the treat-ment group after treatment (χ2=4.615, P=0.032). The retention volume of epiglottic vallecula and pyriform sinus (tdilute barium=21.01, tthick barium=19.85), the duration of pharynx period (tdilute barium=17.03, tthick barium=13.68), and the start time of pharynx period (tdilute barium=19.63, tthick barium=17.86) were less in the treatment group than in the control group (P<0.05). In the test of dilute barium, the aspiration rate was lower in the treatment group than in the control group (χ2=4.03, P=0.044), however, there was no significant difference between 2 groups in the test of thick bari-um and solid food (P>0.05). Conclusion Acupuncture combined doctor-nurse cooperation mode may decrease the rates of aspiration and stroke-associated pneumonia in acute stroke patients through improving their swallow function.

Objective To observe the influence of acupuncture treatment on parturition process.Methods 545 pregnant women delivered in our hospital were selected,excluding those with cesarean section indication.On voluntary principle and randomized principle,primipara aged from 22 to 30 who agreed with natural labor were divided into two groups:observation group and control group.Pregnant women in observation group were received acupuncture treatment during latent phase.The following indexes of the two groups were recorded and compared:labor time of the first stage and the second stage,the case for inertia of uterus,cesarean sections,postpartum hemorrhage amount and Apgar score of new-born.Results The labor time of the first stage and the second stage of the control group were (178.55 ± 56.87) min and (130.70 ± 77.22) min,and which of the observation group were (53.87 ±22.33) min and (33.25 ± 15.55) min,there was significant differenec between the two groups(t =3.97,4.11,all P ＜0.05).The differences in the pilot failed to cesarean section rate(14.2％ and 13.6％),postpartum hemorrhage and the perinatal outcome were not significant between the two groups.Conclusion Acupuncture and moxibustion can accelerate labor.