1.Depression and pedicled omental packing for the treatment of adult polycystic kidney
Chaozhao LIANG ; Kexiao WANG ; Xiaochun KONG
Chinese Journal of Urology 2001;0(10):-
Objective To evaluate depression and pedicl ed omental packing for the treatment of adult polycystic kidney. Met hods Depression and pedicled omental packing was carried out for 16 patients with polycystic kidney. Results Success has bee n achieved in all and 14 patients have been followed up for 1 month to 6 years. Conclusions Depression and pedicled omental packing for the treatment of adult polycystic kidney is feasible and yields good result.
2.Expressions of Th1-Th2-Th17 cytokines in mice chronic viral myocarditis
Xiaochun ZENG ; Qing KONG ; Weifeng WU
Chinese Journal of Immunology 2015;31(12):1601-1604
Objective:To investigate the expressions of Th 1-Th2-Th17 cytokines in the coxsackievirus B 3-induced mice chronic viral myocarditis(VMC).Methods:BALB/c mice were intraperitoneally(i.p) infected with increased CVB3 for establishing chronic VMC models.Control mice were treated with phosphate-buffered saline(PBS)i.p.Cardiac tissues were obtained 8 weeks after CVB3 in-jection,myocardial histopathologic changes were observed by HE and Masson staining .Th1-Th2-Th17 cytokines in plasma were detected by protein array technology , and their cardiac mRNA expressions were measured by RT-PCR.Results: Compared with the control group,levels of IL-2,IL-5,IL-10,IL-13,IL-17,IL-6,IL-22,IL-21 and TGF-βobviously increased in chronic VMC group (P all<0.05). Conclusion:The imbalance of Th1-Th2-Th17 cytokines may play an important roles in the pathogenesis of chronic VMC .
3.Effect of serum carrying Fortune's drynaria rhizome chromocor on proliferation, differentiation, and mineralization of osteoblasts of new-born SD rats
Xiaochun SHU ; Junjing LIU ; Mei KONG ; Ming QIAN ; Lijuan ZHANG ; Ying WANG
Chinese Journal of Endocrinology and Metabolism 2010;26(7):591-594
Objective To study the effect and mechanism of serum carrying Fortune's drynaria rhizome chomocor on proliferation, differentiation, and mineralization of osteoblast of new-born SD rats. Methods (1) Preparation of serum carrying Fortune's drynaria rhizome chromocor: Fortune's drynaria rhizome chromocor was administered once everyday for continuous 12 days to 12 months old female SD rats. Blood was obtained from aorta. (2) Creation of the experiment model; Isolated the osteoblast from the neonatal SD rats' calvaria by using enzyme digestion twice, subcultured. The third passage of cultured osteoblast was chosen as the experiment model. (3) Index; ①The proliferation capacity of osteoblast: detected by method of MTT. ②The differentiation activity of osteoblast: detected by the alkaline phosphatase assay (PNPP) and the amount of ostecalcin (radioimmunoassay). ③The mineralization activity of osteoblast: detected by counting mineral deposites under 40× microscope after chinalizarin staining. Results Serum carrying Fortune's drynaria rhizome chromocor stimulated proliferation of osteoblast of new-bom SD rats in vitro, promoted the excretion of alkaline phosphatase and osteocalsin, and increased the formation of the mineral deposites. Conclusions Fortune's drynaria rhizome chromocor can stimulate proliferation, differentiation, and mineralization of osteoblasts and promote the bone formation.
4.Research on therapeutic effect and hemorrheology change of berberine in new diagnosed patients with type 2 diabetes combining nonalcoholic fatty liver disease.
Xiaoming XIE ; Xiaojun MENG ; Xiaojun ZHOU ; Xiaochun SHU ; Hongjuan KONG
China Journal of Chinese Materia Medica 2011;36(21):3032-3035
OBJECTIVETo explore the therapeutic effect and the hemorrheology change of berberine in new diagnosed patients with type 2 diabetes combining nonalcoholic fatty liver disease.
METHODSixty patients, in our department from March 2009 to March 2010, with type 2 diabetes and nonalcoholic fatty liver disease were randomly divided into two groups. One group was given berberine, another group was given Xuezhikang, both for 12 weeks. The indicators, include B-ultrasound of liver, triglycerides (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), alanine aminotransferase (ALT), aspertate aminotransferase (AST), hemorrheology, were detected before and after treatment.
RESULTAfter treatment by berberine, B-ultrasound of liver were better than before, the effective rate was 70%, vs 73.3% after treatment by Xuezhikang. ALT, AST, TC, TG, LDL-L, hemorrheology (including the whole blood viscosity, whole blood viscosity, high cutting reduction of whole blood viscosity, plasma cutting reductive low viscosity, blood sedimentation, RBC deposited, fibrinogen) were significantly lower than before, however, HDL-L significantly increased (P<0.05). The therapeutic effect of xuezhikang was the same as berberine. The distance between the indicators of the two groups was no different.
CONCLUSIONBerberine can obviously improve the conditions of new diagnostic T2DM patients with nonalcoholic liver lesions, effectively reduce hemorrheology indicators, and has good application prospect.
Adult ; Alanine Transaminase ; blood ; Berberine ; therapeutic use ; Diabetes Mellitus, Type 2 ; blood ; diagnosis ; drug therapy ; Drugs, Chinese Herbal ; therapeutic use ; Fatty Liver ; blood ; diagnosis ; drug therapy ; Female ; Humans ; Lipoproteins, HDL ; blood ; Male ; Middle Aged ; Non-alcoholic Fatty Liver Disease ; Treatment Outcome ; Triglycerides ; blood
5.Three-dimensional transesophageal echocardiography evaluation of changes in parameters of mitral value pre- and post-left atrial appendage closure
Yashu XIE ; Dehong KONG ; Yingying JIANG ; Haiyan CHEN ; Xiaochun ZHANG ; Daxin ZHOU ; Xianhong SHU ; Cuizhen PAN
Chinese Journal of Ultrasonography 2021;30(9):758-763
Objective:To investigate the relationship between Watchman occluder and structure of mitral valve apparatus in patients with nonvalvular atrial fibrillation after left atrial appendage closure(LAAC) by transesophageal echocardiography.Methods:From January 2018 to December 2020, 29 patients [20 male, 9 female, (69.55±10.06)years old] with nonvalvular atrial fibrillation underwent LAAC in Zhongshan Hospital (implanted Watchman occluder), and all patients underwent pre-operative and follow-up two and three dimensional transesophageal echocardiography(2DTEE, 3DTEE) at 60 days after the operation. The quantitative parameters of mitral valve apparatus were obtained by offline analysis using the MVA module in QLab 13.0 (Philips Healthcare, Andover, MA), the differences between pre-operation and post-operation were compared, and the relationship between occluder compression ratio and mitral valve parameters with significant changes after operation was analyzed.Results:①The values of AL-PM diameter(AL-PM), 3D annulus circumference(3DAC), 2D annulus area(2DAA), 3D annulus area(3DAA), tenting volume(TnV), tenting area(TnA) and commissural diameter(CD) decreased significantly compared with pre-operative values(all P<0.05), while the annulus sphericity index(SPI) increased significantly ( P<0.05). ②In the quantitative mitral value parameters with significant pre- and post-operation changes, TnV was correlated with the occluder compression ratio ( r=0.403, P=0.030), but AL-PM, SPI, 3DAC, 2DAA, 3DAA, TnA, CD were not correlated with it(all P>0.05). Conclusions:3DTEE can accurately evaluate the effect of Watchman occluder on the morphology of mitral valve device. Implanting Watchman occluder in left atrial appendage can make three-dimensional mitral valve apparatus flat and decrease annulus left-right diameter and annulus area; the pre-operative TnV, the more susceptible to the occluder implantation, and TnV is correlated with the compression ratio.
6.Laboratory test results of confirmed COVID-19 cases in Tianjin and related influencing factors
Xiaoyan LI ; Xin GAO ; Liru GUO ; Likun LYU ; Yue LEI ; Xiaochun DONG ; Mei KONG ; Ming ZOU ; Wen LI ; Xu SU
Chinese Journal of Microbiology and Immunology 2020;40(5):330-335
Objective:To study the characteristics and influence factors of laboratory test results of confirmed COVID-19 cases in Tianjin.Methods:Sample collection was conducted based on the standard operating procedure. Tianlong automatic magnetic bead nucleic acid extraction reagent was used for RNA extraction. Real-time RT-PCR was performed using four approved COVID-19 nucleic acid detection kits. Related epidemiological data of the cases were collected. One-way analysis of variance and non-parametric test for inter-group differences analysis were conducted using SPSS25.0 software.Results:A total of 162 PCR tests were completed for novel coronavirus nucleic acid detection in 123 confirmed COVID-19 cases. Eleven PCR results were positive for a single target gene and 10 of which were positive for nucleocapsid protein (N) gene. Nineteen cases were tested with two kinds of nucleic acid detection kits and the results of different detection kits were different. Different types of samples were collected form 13 cases for nucleic acid detection and the results showed that the Ct value of sputum sample was lower than that of throat swab sample. No significant difference in Ct values of throat swab samples was observed among patients with different clinical symptoms ( PCt-N=0.797, PCt-ORF1a/b=0.551). The 123 cases were divided into different groups according to the time interval between the onset date and the date of the first positive detection of viral nucleic acid. No significant difference in Ct values of throat swab samples was observed among different time interval groups ( PCt-N=0.373, PCt-ORF1a/b=0.058). Conclusions:Sputum samples were better than upper respiratory tract samples for viral nucleic acid detection. The sensitivity of N gene detection was higher, but re-sampling was needed when the result was positive for the single target N gene. Appropriate detection kits should be selected according to the actual needs, and samples should be collected at multiple time points, in multiple types and form multiple sites for detection.
7.One case report of repeated chest tightness and palpitation cured after strengthening calcium supplementation
Xiaochun CHENG ; Liyuan MU ; Lingquan KONG ; Fan LI ; Hongyuan LI ; Guosheng REN ; Kainan WU
Chinese Journal of Endocrine Surgery 2024;18(1):147-148
The incidence of parathyroid hyperfunction is high and its clinical manifestations are diverse. Some patients develop chest tightness and palpitations as the main discomfort, which may be caused by the hypocalcemia and hypercalcemia related to negative calcium balance and parathyroid hyperfunction. We report a case of 53 years old male with parathyroid hyperfunction who was diagnosed with osteoporosis before and received conventional regular supplementation of vitamin D and calcium supplements. However, his condition worsened and he developed chest tightness and palpitation. After 1 month of sufficient supplementation of calcium, the symptoms of chest tightness and palpitation disappeared completely. Then we continued to provide the patients enough vitamin D and calcium supplementation actively. After 1 year of follow-up, the patient's condition was stable. His discomfort of chest tightness and palpitation never recurred, and all the bone metabolism indicators returned to normal.
8.Impact of the LAmbre device on left atrial appendage adjacent structures and left atrium
Zhengdan GE ; Dehong KONG ; Zhenyi GE ; Chunqiang HU ; Xiaochun ZHANG ; Haiyan CHEN ; Daxin ZHOU ; Xianhong SHU ; Cuizhen PAN
Chinese Journal of Ultrasonography 2024;33(2):142-150
Objective:To explore the effects of the LAmbre device and mitral annulus(MA), as well as left atrium(LA) in patients with non-valvular atrial fibrillation(NVAF) after left atrial appendage closure (LAAC) using real-time-three-dimensional transesophageal echocardiography (3D TEE).Methods:Fity-six consecutive patients who underwent LAAC with the LAmbre device in Zhongshan Hospital of Fudan University from June 2019 to March 2023 were retrospectively enrolled, with no or less than moderate mitral regurgitation (MR). All patients underwent pre-operative and follow-up two-and three-dimensional transesophageal echocardiography (2D TEE, 3D TEE) at 60 days after the operation. The quantitative parameters of MA and LA were obtained by offline analysis using QLab 13.0 (Philips Healthcare, Andover, MA). Importantly, mitral annular measurements were made at seven time points throughout the cardiac cycle: early diastole, mid-diastole, late diastole, mitral valve closure, early systole, mid-systole, and late systole, which facilitates constructing the dynamic model of MA to assess the annular morphology and dynamics.Results:The values of AP diameter (APD), AL-PM diameter (ALPMD), 3D annulus circumference (3DAC), 3D annulus area (3DAA) decreased significantly compared with pre-operative values at all time points of the cardiac cycle (all P<0.05), while non-planar angle (NPA) and AH/CD were not apparently changed (all P>0.05 ). Throughout the cardiac cycle, MA showed regular changes, gradually increased in systole with the saddle shape deepened, and gradually decreased in diastole with the saddle shape shallowed.During systole, there was an increase in the rate of change of AP in MA [pre-operative (3.01±2.64)%, post-operative (3.81±3.51)%, P=0.037] after LAAC, with no significant difference in the rate of change of ALPM, 3DAC, and 3DAA.Meanwhile, we observed an evident reduction in LA minimal volume (LAVmin) [pre-operative (78.36±25.16)ml, post-operative (70.73±22.78)ml, P=0.004] and an obvious increase in LA ejection function [pre-operative (22.88±10.09)%, post-operative (31.41±12.28)%, P<0.05] during follow-up. Conclusions:3D TEE can accurately assess the impact of LAAC on the MA and LA. The LAmbre device can affect the morphology of MA, as well as the structure and function of LA, while the change of the dynamics of MA is not so prominent.
9.Real-time monitoring and step-by-step guidance for transcatheter tricuspid annuloplasty using transesophageal echocardiography
Cuizhen PAN ; Daxin ZHOU ; Xiaochun ZHANG ; Wei LI ; Shasha CHEN ; Yuan ZHANG ; Jing SHI ; Haiyan CHEN ; Dehong KONG ; Yu LIU ; Zhenyi GE ; Kefang GUO ; Xianhong SHU ; Junbo GE
Chinese Journal of Ultrasonography 2022;31(7):626-630
Tricuspid regurgitation (TR) interventions are under rapid development. The K-Clip? system is the first domestic transcatheter tricuspid annuloplasty system with unique clamping procedure to achieve annular reduction.Intraoperative echocardiographic monitoring procedures for transcatheter tricuspid annuloplasty have not been reported yet in China. Thus, this review aimed to propose the standard two-dimensional and three-dimensional transesophageal echocardiographic workplanes and procedures to guide and monitor the implantation of K-Clip system based on our experience in Zhongshan Hospital, Fudan University to provide a reference point for the intraoperative echocardiographic monitoring of future transcatheter tricuspid annuloplasty devices in China.