1.Expression of macrophage inlfammatory protein 1αin mice with viral myocarditis
Journal of Clinical Pediatrics 2013;(10):964-967
Objective To detect the expression of macrophage inlfammatory protein 1α(MIP-1α) in the myocardium of viral myocarditis (VMC) mice at different phases. Methods A total of 120 4-week-old male BALB/c mice were randomly divided into 2 groups, 80 in the VMC group and 40 in the control group. Mice in VMC group were inoculated intraperitoneally with coxsackievirus B3 to build VMC models, while mice in control group were treated with DMEM cultivate liquid. Ten mice of each group were sacriifced on days 3, 7, 15 and 30 after treatment and their heart tissues were collected for analysis. The level of MIP-1αin the myocardium was determined by immunohistochemistry. Myocardial histopathology was examined with hematoxylin and eosin stain. In addition, the relationship between the level of MIP-1αand the degree of myocardial lesion was investigated. Results The expression of myocardial MIP-1αprotein in VMC group was up-regulated in myocardium on day 3 after inoculation of virus, and slowly decreased after the peak on day 7, but still sustained a high level on day 30. Compared with the control group, the levels of MIP-1αin VMC group were increased signiifcantly at every phase (P<0.05). Furthermore, positive correlation was found between MIP-1αprotein expression levels and myocardial histopathologic scores in VMC group (r=0.94, P<0.01). Conclusion The up-regulated expression of MIP-1αmay play a critical role in the pathogenic mechanisms of viral myocarditis.
2.The present situation and future of transplantation therapy for follicular lymphoma in the time of rituximab
Journal of Leukemia & Lymphoma 2013;22(6):335-338
Follicular lymphoma (FL) is a subtype of B cell lymphoma,which derived from follicular germinal center.As the most common type of indolent lymphoma,the overall prognosis of FL is good.However,the prognosis of FL is heterogeneous.With the application of rituximab,the therapeutic effect and the prognosis of the disease is further improved.For the relapsed / refractory patients,especially who have received first-line chemotherapy with rituximab in the early,transplantation is an important choice.Compared with the previous conventional myeloablative conditioning regimen,reduced-intensity conditioning (RIC) effectively reduced the non-relapse mortality (NRM),extended the allogeneic transplantation recipients,but the high rate of relapse after transplantation is the main defect.The application of donor lymphocyte infusion after transplantation therapy not only can reduce the recurrence rate but also treat the disease relapse.In the future,the improvement of transplantation strategy and the application of new drugs may bring new ideas and opportunities for FL therapy.
3.Curriculum Construction of Occupational Therapy Technique in Higher Vocational Education
Xuezhen MA ; Xiaochen WANG ; Yi CHEN
Chinese Journal of Rehabilitation Theory and Practice 2012;18(8):789-790
Occupational therapy technique is one of the core curriculums of rehabilitation treatment profession. Curriculum construction of occupational therapy technique will have a direct impact on students' quality. This article introduced the reform in the process of curriculum construction from aspects including the curriculum standards, textbooks, teaching methods and course evaluation.
4.Reform of Teaching for Higher Vocational Rehabilitation Therapy
Yi CHEN ; Xuezhen MA ; Xiaochen WANG
Chinese Journal of Rehabilitation Theory and Practice 2013;19(3):296-297
This article introduced the reform of the teaching mode for Rehabilitation Therapy in higher vocational education, including the course of theory and practice, system of personnel training and assessment.
5.Task-driven Teaching Mode for Physical Therapy in Higher Vocational Education
Xiaochen WANG ; Yi CHEN ; Xuezhen MA
Chinese Journal of Rehabilitation Theory and Practice 2010;16(5):497-498
ObjectiveTo investigate the effects of task-driven teaching mode on physical therapy teaching in higher vocational education. Methods40 students in 2006 rehabilitation class were taught with routine mode as the control group, while 35 students in 2007 rehabilitation class taught with task-driven mode and routine mode as experimental group. ResultsAt the end of the course, the results of examination in theory and practice were better in the experimental group than control group (P<0.01). 85.71%~94.28% students in the experimental group said that the task-driven teaching mode could improve their comprehensive quality especially the skill of practice, as well as the interest and ability of learning. ConclusionTask-driven teaching mode can improve the teaching effect on physical therapy, and received by most of the students.
6.Pyrolytic depolymerization mechanism of a lignin model compound with α-O-4 linkage.
Xiaoyan JIANG ; Qiang LU ; Xiaochen DONG ; Chen CHEN ; Changqing DONG
Chinese Journal of Biotechnology 2015;31(10):1512-1519
To understand the pyrolysis mechanism of lignin with α-O-4 linkage, 4-(3-hydroxy-1-phenoxypropyl)-phenol was selected as an α-O-4 type lignin dimer model compound, and its pyrolysis process was studied by density functional theory with M06-2X method at 6-31+G (d,p) level. Equilibrium geometries of the reactant, intermediates, transition states and products were fully optimized. The activation energies in each pyrolysis pathway were calculated. The dimer decomposed mainly through the homolytic cleavage and concerted decomposition of the C(α)-O linkage. Pyrolytic products mainly included various phenolic compounds such as phenol, 4-methylphenol, 4-vinylphenol and p-coumaryl alcohol, as well as light compounds such as ethanol, methanol and formaldehyde. Pyrolytic depolymerization process has its potential in biomass-based fuels.
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chemistry
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Ethanol
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chemistry
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Formaldehyde
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chemistry
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Hot Temperature
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Lignin
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chemistry
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Methanol
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Phenols
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Propionates
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chemistry
7.The inoculum effect of fosfomycin in tests with Enterococcus
Xiaochen WANG ; Xin ZHANG ; Tianshi CHEN ; Baiyi CHEN
Chinese Journal of Infectious Diseases 2015;33(2):87-90
Objective To investigate the inoculum effect of fosfomycin on enterococci,in comparison with penicillin,rifampin,vancomycin and teicoplanin.Methods A total of 91 strains of enterococci were obtained from the patients hospitalized in The First Affiliated Hospital of China Medical University from January 2010 to January 2012.There was no repeated strain.The 91 strains consisted of 44 Enterococcus faecalis and 47 Enterococcus faecium strains.Agar dilution method was used to measure the minimum inhibitory concentrations (MIC) of the five antibiotics against the 91 enterococci strains under standard low inoculation concentration (1 × 105 colony-forming units [cfu]/mL) and high inoculation concentration (1 × 107 cfu/mL).Results For all tested strains,MIC of fosfomycin fluctuated between 16-64 tμg/mL in high and low inoculated concentrations.Among the tested strains,both Enterococcus faecalis and Enterococcus faecium had the highest drug-sensitive rate (100.0%) and the lowest drugresistance rate (0) to fosfomycin.With the increase of the inoculation concentration,MIC of the five antibiotics increased less than 8 times,which indicated that there was no inoculation effect.Conclusion Fosfomycin shows high drug-sensitivity against enterococci without inoculum effect in vitro,and it is recommended for the treatment of enterococci-infected patients.
8.Prometastatic overexpression of microtubule destabilizing protein Stathmin is regulated by DNA methylation in human pancreatic cancer
Chen LIU ; Xiaochen CAI ; Jiang LONG ; Xianjun YU ; Quanxing NI
Chinese Journal of Hepatobiliary Surgery 2012;18(6):442-446
Objective To investigate the role of Stathmin in pancreatic cancer invasion and metastasis and its relationship with DNA methylation. Methods Immunohistochemical detection of MBDI and Stathmin protein expression in 40 cases of pancreatic cancer and 15 cases ot normal pancreatic tissue were performed,followed by analysis of their clinical and pathological relationship with pancreatic cancer; Human pancreatic cancer cell line BxPC-3 was treated with 5-Aza-2-dC (AZA).Both qRT-PCR and Western blot analysis of Stathmin expression were used before and after AZA treatment; Stathmin-siRNA transfected BxPC-3 cells were divided into the Stathmi-siRNA group and the empty vector control group.Transwell chamber invasion assay and animal experiment were performed to measure the changes in cell invasion and metastatic capability. Results lmmunohistochemistry showed positive MBDI and Stathmin expressions in 28 (70%) and 24 (60%) out of 40 cases of pancreatic cancer,respectively,which were significantly higher than that in the normal pancreatic tissue (P< 0.05); MBDI and Stathmin protein expressions were positively correlated (r =0.356,P =0.037),so were MBDI expression and lymph node metastasis (P=0.023).Stathmin expression was significantly correlated with clinical staging and lymph node metastasis (P =0.002,and P =0.001,respectively).After AZA treatment,both Stathmin mRNA and protein expression in BxPC-3 were significantly decreased.Transwell chamber invasion assay showed that compared with the control group,the cell invasion capability of the Stathmin-siRNA group was significantly decreased (P<0.05).Animal experiment showed that the incidence of liver metastasis was significantly lower in the Stathmin-siRNA transfected group than the empty vector control group (P<0.05).Conclusion Demethylation may contribute to the reduction of Stathmin expression in pancreatic cancer and further improve the prognosis of pancreatic cancer patients.
9.Effects of Zhiyu Granules on sciatic nerve function of diabetic peripheral neuritis
Xiaochen ZHANG ; Yi YUAN ; Yihe CHEN ; Wenjuan WANG
Chinese Traditional Patent Medicine 1992;0(08):-
AIM: To discuss the mechanism of action of Zhiyu Granules on treating diabetic peripheral neuritis. METHODS: The model of diabetic peripheral neuritis in mice was induced by streptozotocin.The effect of Zhiyu Granules on the contents of sciatic nerve inositol in model mice through ELSD(evaporative light scattering detector) was examined,and the activity of Na~+-K~+ pump in sciatic nerve was measured,and the activity of SOD and the contents of MDA in erythrocytes were detected,and the ultrastructures of sciatic nerve,pancreas and kidney in model mice were observed by electric microscope. RESULTS: Zhiyu Granules could increase the contents of sciatic nerve inositol in model rats,and enhance the activity of Na~+-K~+ pump,and improve the ultrastructures of sciatic nerve and pancreas cell.Zhiyu Granules couldn't improve the ultrastructure of renal corpuscles,or affect the activity of SOD and the contents of MDA in erythrocytes of model mice yet. CONCLUSION: Zhiyu Granules is effective in clinical treatment of diabetic peripheral neuritis.
10.Gelatin sponge particlesversus chitosan/alpha, beta-glycerophosphate gel microspheres in hemoptysis of pulmonary tuberculosis
Ping JI ; Zhe CHEN ; Xiaochen SUN ; Long YUAN
Chinese Journal of Tissue Engineering Research 2017;21(18):2876-2880
BACKGROUND:It is reported that gelatin sponge particles and chitosan/α, β-glycerophosphate gel microspheres have high application prospects in the treatment of hemoptysis of pulmonary tuberculosis.METHODS: Forty SPF Kunming mice were selected to make animal models of pulmonary tuberculosis hemoptysis using aerosolized inhalation device and randomly divided into two groups undergoing embolization for hemostasis using gelatin sponge particles and chitosan/α, β-glycerophosphate gel microspheres, respectively. Body mass changes, hemostasis time, effective rate of hemostasis, related indexes of myocardium and pathological changes of pulmonary tuberculosis were observed at 15 and 30 days after treatment.RESULTS AND CONCLUSION:(1) Mice in the chitosan/α, β-glycerophosphate gel microspheres group had higher body mass than those in the gelatin sponge group at 15 days after treatment (P < 0.05). (2) Compared with the gelatin sponge group, the hemostatic efficiency was higher and the time of hemostasis was shorter in the chitosan/α, β-glycerophosphate gel microspheres group (P< 0.05). (3) There were no significant differences in the levels of BNP precursor, creatine kinase isoenzyme and creatine kinase between the two groups (P > 0.05). (4) The lymphocyte infiltration and necrotic lesions were found around the microspheres, necrotic lesions were found, and there were a lot of active cells on the microspheres. There were a few lymphocytes in the gelatin sponge group. These results show that that the chitosan/α, β-glycerophosphate gel microspheres have better hemostatic effects in comparison with gelatin sponge particles in a mouse model of pulmonary tuberculosis.