1.APPLICATION OF RPLC TO THE SEPARATION AND DETERMINATION OF VITAMIN B_6-PYRIDOXAMINE, PYRIDOXAL, PYRIDOXINE
Yiping REN ; Baifen HUANG ; Xiaocan WU ; Yun ZHOU
Acta Nutrimenta Sinica 1956;0(03):-
A method for the determination of B6-pyridoxamine (PAM), pyridoxal (PAL) and pyridoxine (POL) by RPLC was proposed. The procedure included the addition of 0.1M H2SO4 to the sample, hydrolysis for 30 min at 120℃, centrifuging, filtration and direct analysis by ODS Cl8 column. PAM, PAL and POL were completely separated, when the flow rate of the mobile phase (pH2.0-2.1) was 1.0-1.5ml/min. Quantitation by a fluorescent detector was performed with Exc: 293 nm and Em: 395 nm. The recovery ranged from 99 to 103% with CV 5.0-5.3%. The method was simple, rapid, sensitive and reproducible.
2.Analysis on the risk factors affecting the early postoperative outcome in patients of infants and young children with tetralogy of Fallot
Xiaocan WEI ; Chun WU ; Zhengxia PAN ; Yonggang LI ; Yong AN ; Hongbo LI ; Jiangtao DAI ; Gang WANG
Chongqing Medicine 2013;(36):4400-4401,4404
Objective Analysis the influence risk factors of infants and young children tetralogy of Fallot for radical treatment , and explore the perioperative treatment methods .Methods 195 cases(include death group and survival group)of hospitalized data of TOF resection in this hospital were collected in January 2003 to November 2012 ,then statistical analysis was done .Results Uni-variate analysis of variance showed ,age ,weight ,McGoon ratio ,cardiopulmonary bypass time and aortic clamping time were statisti-cally significant in two groups ,Multivariate Logistic regression analysis showed McGoon ratio <1 .0 ,cardiopulmonary bypass time>90 min ,aortic clamping time>70 min ,age<3 months were related to the postoperative death of TOF radical operation .Conclu-sion It is safe and reliable of radical surgery in infants and young children ,McGoon ratio ,cardiopulmonary bypass time and aortic clamping time ,age are the risk factors of the postoperative death of TOF radical operation .