Objective:To observe the effect of electroacupuncture(EA)combined with routine language and motor training on limb and language function,depression,and serum brain injury markers in patients with ischemic stroke. Methods:A total of 86 patients with ischemic stroke were selected and divided into an observation group and a control group using the random drawing method,with 43 cases in each group.Both groups received routine stroke treatments.The control group was additionally treated with routine language and motor training,and the observation group was additionally treated with EA treatment on the basis of the intervention of the control group.Changes in the limb function score,language function score,depression score,and brain injury marker protein were compared between the two groups,and the treatment safety of the two groups was evaluated. Results:The Fugl-Meyer assessment scale scores of both groups were higher than those before treatment(P<0.05),and the Fugl-Meyer assessment scale score of limb function of the observation group was higher than that of the control group(P<0.05).The language function score of the observation group was higher than that of the control group after treatment(P<0.05).The Montgomery-Asberg depression rating scale(MADRS)scores of the observation group were lower than those of the control group after treatment(P<0.05).The levels of brain injury markers heart-type fatty acid-binding protein(H-FABP),glial fibrillary acidic protein(GFAP),and annexin a7(ANXA7)of the observation group were lower than those of the control group(P<0.05).The incidence rate of adverse reactions in the two groups was 14.0%and 9.3%,respectively,with no statistically significant difference between the groups(P>0.05). Conclusion:EA combined with language and motor training is effective in the treatment of ischemic stroke.It can promote the recovery of limb motor function and language function,inhibit the occurrence of depression,and reduce the levels of brain injury markers,with good safety.

Objective The aim of this study was to observe the outcome and evaluate the clinical efficacy of pulsed electromagnetic fields (PEMF) on pain management in patients with osteoporosis.MethodsA total of 58 patients with osteoporosis were treated with PEMF stimulation.Pains as main indicators of their symptom were evaluated before and after treatments.The clinical outcomes were assessed by observing the visual analogue scale (VAS).Statistical analysis was carried out to support the data.Results After PEMF treatment,the patients exhibited significant pain relief from mean VAS of 6.97±1.83 to 3.36±1.69(t-test,P＜0.05 ).Of the 58 patients,28 obtained significant symptom alleviation and 18 obtained moderate symptom alleviation,while 8 obtained slight alleviation,indicating the total clinical efficacy of 93%(54/58).Conclusion The current study provides the clinical outcomes indicating that PEMF may help to release the pain from primary osteoporosis and it might be an applicable supplementary treatment method for the osteoporosis related pain.

Objective Dipole source analysis was employed to investigate the transient changes in brain mechanisms at earlier latencies.Methods Fourteen healthy volunteers were recruited in this research and evoked event-related potentials (ERPs) of unimodal and bimodal visual auditory stimuli were recorded by 64-electrodes electroencephalograph (EEG) recording system.All these earlier phases of the stimuli were divided into several subphases by specific time window for source analysis.Results The results showed that ERPs sources were mainly generated from visual and audio cortex,and there were changes in the location and strength of the dipole sources in each sub-phase.Conclusions The result of this study implies a serial processing of sensory information in human cortices in early phase of visual and auditory stimuli.

ObjectiveTo design multi-adaptive filter based on radial basis function (MAF-RBF) for efficiently extracting somatosensory evoked potential (SEP) in real-time SEP monitoring.MethodsWith the optimization of important parameters that influence the performance of radial basis function neural network,the performance of extracting SEP was compared to that of a multi-adaptive filter (MAF),which developed from the combination of well-developed adaptive noise canceller and adaptive signal enhancer.ResultsIn this simulation study,the outputs of MAF-RBF showed a similar waveform with SEP template signals,and a smoother waveform than the.output of MAF.ConclusionWith appropriate parameter values,MAF-RBFNN is able to extract the latency and amplitude of SEP from the extremely noisy background rapidly and reliably without averaging.

Objective To study the association of the serum alanine aminotransferase (ALT) level with cardiovascular risks in patients with type 2 diabetes mellitus (T2DM).Methods A total of 676 patients with T2DM who excluded liver disease due to chronic viral hepatitis,autoimmune,drugs,alcohol,and other hereditary were selected.According to the ALT level,676 patients were assigned into elevated ALT group (ALT ＞40 U/L,126 cases) and normal ALT group (ALT 5-40 U/L,550 cases).And normal ALT group were divided into 4 groups on the interquartile distance:group 1 (ALT 5-11 U/L,108 cases),group 2 (ALT 12-15 U/L,114 cases),group 3 (ALT 16-21 U/L,127 cases),group 4 (ALT 22-40 U/L,201cases).The age,duration of diabetes,body mass index (BMI),waist-to-hip ratio (WHR),blood pressure,triglyceride (TG),total cholesterol (TC),high density lipoprotein cholesferol (HDL-C),low density lipoprotein cholesferol (LDL-C),fasting plasma glucose (FPG),2 h postprandial blood glucose (2 hPG),glycosylated hemoglobin (HbA1c),uric acid (UA),fasting insulin and homeostasis model assessment insulin resistance index (HOMA-IR) was compared among these groups.Results The levels of BMI,WHR,diastolic blood pressure,FPG,2 hPG,HbA1c,fasting insulin,TG,HOMA-IR and UA in elevated ALT group were higher than those in normal ALT group [(26.80 ± 3.60) kg/m2 vs.(23.40 ± 3.50) kg/m2,0.95 ±0.05 vs.0.91 ± 0.05,(75.3 ± 9.3) mm Hg (1 mm Hg =0.133 kPa) vs.(69.2 ± 9.5)mm Hg,(9.24 ± 2.56)mmol/L vs.(8.53 ± 2.78) mmol/L,(15.25 ± 4.62) mmol/L vs.(14.37 ± 5.06) mmoUL,(8.31 ± 1.76)％ vs.(7.78 ± 1.94)％,(10.76 ± 6.78) mU/L vs.(7.85 ± 7.04) mU/L,(2.73 ± 2.40) mmol/L vs.(2.20 ± 2.78)mmol/L,4.13 ± 2.76 vs.3.06 ± 2.63,(332.42 ± 95.12) μ mol/L vs.(285.14 ± 79.53) μ mol/L] (P ＜ 0.05),and HDL-C was lower than that in normal ALT group [(1.06 ± 0.27) mmol/L vs.(1.15 ± 0.57) mmol/L] (P ＜0.05).Compared with that in normal ALT group,the age was younger,and the duration of diabetes was shorter in elevated ALT group [(49.6 ± 10.5) years vs.(57.5 ± 11.3) years,(37.9 ±42.0) months vs.(54.5 ± 57.8) months] (P ＜ 0.05).The levels of FPG,2 hPG,HbA1c and UA in group 4 were higher than those in group 1,2,3 [(9.18 ± 3.01) mmol/L vs.(8.33 ± 2.57),(8.38 ± 2.52),(8.45 ± 2.73) mmol/L;(14.56 ± 4.80) mmol/L vs.(13.15 ± 5.72),(13.42 ± 4.56),(13.50 ± 5.17) mmol/L; (7.81 ± 1.98)％ vs.(6.76 ± 1.84)％,(6.79 ± 2.10)％,(6.88 ± 2.43)％; (288.24 ± 78.26) μ mol/L vs.(271.15 ± 75.43),(273.27 ± 71.25),(275.56 ± 69.57) μ mol/L] (P ＜ 0.05) ; HOMA-IR was lower than that in group 1,2,3(2.67 ± 2.65 vs.2.84 ± 2.53,2.98 ± 3.10,3.12 ± 2.57,P ＜ 0.05).Conclusion TheincreaseofALTlevel is associated with the clustered cardiovascular risk factors and insulin resistance in patients with T2DM.

Objective To explore the correlation between HIF-1α expression and RANKL/OPG pathway activation of fibroblast-like synoviocytes(FLSs)induced by Ti particles in aseptic loosening interfacial membrane. Method FLSs were extracted from the synovial tissue collected in surgeries and then co-cultured with Ti particles. QRT-PCR and Western blotting were conducted to measure the mRNA and protein expression of RANKL ,OPG and HIF-1αin FLSs at different concentration and time. Results Genes and protein expression levels of RANKL/OPG and HIF-1α were up-regulated with the increase of concentration of Ti particles. Expression of HIF-1α gene and protein increased time-dependently;the mRNA and protein expression of RANKL/OPG increased firstly and then declined alongside the increase of HIF-1αexpression. Conclusions Ti particles induce the up-regulation of HIF-1α expression and activate RANKL/OPG pathway. Up-regulation of HIF-1α may suppress the activation of RANKL/OPG pathway in FLSs of the interfacial membrane induced by Ti particles.

BACKGROUND: Frustration tolerance, affected by many factors, is a kind of ability that an individual can endure and overcome the adversity. This research would adopt group therapy, especially the experienced therapy. On the basis of helping clients rebuilt cognition, this research would make clients experience frustration by themselves, increase frustration experience and enhance the threshold value of frustration.OBJECTIVE: To observe the enhancing effect of group therapy on frustration tolerance of postgraduates. DESIGN : Self-control observation. SETTING: Department of Preventive Medicine, Medical College of Jiangsu University. PARTICIPANTS; This experiment was conducted in Jiangsu University. A total of 1200 fresh postgraduates enrolled in 2005 from business administration, computer science and communication engineering, mechanical engineering, medical technology, automobile and traffic and chemistry and chemical engineering colleges were screened with "frustration tolerant questionnaires" from "perfect personality testing handbook". There were 19 questions in this questionnaire. All of these questions were negative, for example, you did not want to compete with others when you were behind others. There were A, B, C, D, E five scales for every question. The different answers will have different scores. A represented extremely coincidence, B represented slight coincidence, C represented unknown, D represented discrepancy, and E represented very discrepancy. Different answers corresponded to different scores. The total scores were given at last. The higher the scores were, the more severe the anxiety scale was. There were 70 postgraduates whose scores of frustration tolerant were below 50, the scores of the other 1 130 postgraduates were equal to or more than 50. Twenty-three students were sampled randomly, whose scores of frustration tolerance were below 50 as an experimental group. At the same time, 44 ones with scores of frustration tolerance over or equal to 50 were sampled as a control group. There were 67 postgraduates in the two groups.METHODS: Sixty-seven postgraduates were divided into 7 groups with 10 in each group according to gender, scores of frustration tolerant and different colleges. We adopted group therapy. Firstly, we chose language induction based on background music for hypnotherapy, and placed the clients at ease and into therapeutic state. After hypnosis, we developed a game "breaking ice" which would help clients understand each other and make a comfortable atmosphere. Next, we carried on the cognitive rebuilt to change the bad cognition of the students, and help them foster the right thought of frustration. Besides, we had an activity "blind looking for road" and a psychodrama and "beastly boss". During the treat ment of "blind looking for road", the students' eyes were blindfolded, and they walked through the campus to experience the hard life in blind. During the play of "beastly boss", one of them acted a boss, and other one acted an employee, then by turns. The story was that a boss scolded an employee in a rough manner, an employee explained, pled, but the boss went on inveighing. In order to avoid being fired, the employee had to chokes down. In the act, the students would taste the frustrated feeling when rebuked innocently, and thus the students would get experience of frustration and enhance their patience. After every activity, we divided them into small and big groups, then let them talk and share, to give utual study and promotion. In the process, we integrated all the approaches and organized them naturally to heighten the effect of the treatment.MAIN OUTCOME MEASURES: ① Comparison of mean scores of frustration tolerance before and after treatment. ② Comparison of mean scores of frustration tolerance before and after treatment among clients with different frustration toler ances.RESULTS: Sixty-seven questionnaires were handed out, and 57 effective questionnaires were collected in total by the end of treatment, and the other 10 questionnaires were invalid due to incomplete filling. Nineteen of effective questionnaires were from experimental group and 38 from control group. The total retrieved rate of questionnaires was 87.7％. The mean scores of frustration tolerance of involved 57 postgraduates before treatment were significantly higher than those after treatment [(54.4±8.90) points vs. (58.70±10.94) points, t =0.38, P ＜ 0.05). The mean scores of frustration tolerance of postgraduates with mean scores of frustration tolerance ＜ 50 points were significantly higher after treatment than before treatment (t =3.49, P ＜ 0.05).CONCLUSION: Group therapy, especially psychodrama and other frustration experience therapies can effectively enhance the frustration tolerance of clients.

ObjectiveTo efficiently detect somsatosensory evoked potential (SEP) using field programmable gate array (FPGA) real-time system, fixed-point algorithm based adaptive noise canceller (ANC) was designed to improve signal to noise ratio (SNR). MethodsWith the optimization of important parameters that influence the performance of fixed-point algorithm ANC, the performance was compared to that of floating-point algorithm ANC which was isolated from the effect of quantization error. Results In the simulation study, the outputs of fixed-point-based ANC showed a little higher distortion from real SEP signals than that of floating-point algorithm ANC. In the optimal selection of μ value, fixed-point algorithm ANC could get as good results as floating-point algorithm. Conclusion With appropriate parameter values, fixed-point algorithm ANC is able to improve SNR of SEP as well as that of fixed-point algorithm ANC.

Objective: To establish the determination of adenosine in Ganping Capsules. Methods: HPLC method was developed for the determination of adenosine in Ganping Capsules on Kromasil C 18 (150mm? 4.6mm i.d.) in 0.05mol/L KH 2PO 4 methanol (88∶12, V/V,pH 4.7) as a mobile phase (1.0mL/min). The UV detection wavelength was set at 260nm. Adenosine was extracted with 10% methanol water. Results: The recoveries was 98%～102% and the linear equation was Y=2537.4X+100.81. The correlation coefficient was r =0.9999(n=5). Conclusion: The method is simple, rapid and accurate.

Objective Establish an experimental model for primary subchondral bone osteoblasts culture of neonatal SD rat's condylar process. Methods Under the condition of sterile,24-hour SD rat was executed and its condylar process was isolated. Removing cartilage layer, the subchondral bone was exposed obviously, then it was cultured with modified repeating enzymatic digestion-adherent explants method. The cellular morphology was identified with invert microscope and immunohistochemistry staining, the osteoblasts were identified by alkaline phosphorase (ALP) staining and calcified nodules staining, and the proliferation of the acquired cells was examined by methyl thiazolyl tetrazolium (MTT) assay. Results A variety of cell morphologies were observed, such as spindle-shaped, triangular and irregular-shape, and their cell processes were significant. The alkaline phosphatase staining and calcified nodules staining of cultured osteoblasts with mineralized nodules were positive. Cells grew slowly in 1-3 days, and the cells growth reached the highest level at the 8th day. The cells growth trend has gradually slowed down after 8 days. Conclusion The method is an efficient way to culture and obtain purified neonatal SD rat's subchondral bone osteoblasts with typical characteristics.