Image-guided minimally invasive treatments play an increasingly important role in clinical works.With the progressing of science and technology,image-guided robot technology are mature gradually.It can accomplish parts of clinical minimally invasive treatments,and make up for the deficiencies in the process of surgery.Present situation and application in minimally invasive treatments of image-guided robot system were reviewed in this article.

Objective:This work aims to explore the long-term efficacy and complications of late-course accelerated hyperfrac-tionation (LCAHF) for treating nasopharyngeal carcinoma. Methods:A total of 58 patients who consulted from December 2005 to May 2008 and histologically proven nasopharyngeal carcinoma at initial diagnosis were randomized into an LCAHF group (experimental group) and a conventional fractionation (CF) group (control group). The treatment dose for both groups was 2 Gy per fraction once dai-ly, 5 days a week. After the 40 Gy to 50 Gy dose, the dosage in the LCAHF group was increased to two daily doses at 1.5 Gy per frac-tion 6 h apart, 5 days a week. The total dose in this group was 73 Gy to 76 Gy, the total dose in the CF group was 70 Gy to 76 Gy, with the total course of the treatment shortened by 0.5 weeks to 1.5 weeks in the former group. Results:The 5-year control rates of the naso-pharyngeal cancers was 86% in the LCAHF group and 59% in the CF group (P=0.021), with statistically significant differences be-tween the two groups. The late complications slightly increased in the LCAHF group than in the CF group, but the differences were not statistically significant. Conclusion:LCAHF treatment improves the local control of nasopharyngeal carcinoma without increasing the incidence of long-term complications.

Objective:To investigate the protective effects of Panax notoginseng on myocardial ischemia injury in dogs and study the spectrum-activity relationship of Panax notoginseng. Methods:Firstly, the HPLC fingerprint analytical method for Panax notogin-seng was established, and then the dog model of acute myocardial ischemia was established by left anterior descending coronary liga-tion. Bivariate correlation analysis and multivariate regression analysis were used to correlate the spectrum-activity relationship between the fingerprints and the anti-myocardial ischaemia activity, and the spectrum-activity relationship and efficacy material foundation of Panax notoginseng were determined. Results:The main effective components were Ginseng saponin Rg1 and Rb1 and notoginseng sapo-nins R1 etc. Notoginseng saponins R1 could significantly inhibit the increase of serum lactate, and ginseng saponin Rg1 could inhibit the increase of FFA in serum, which was the main component in Panax notoginseng for the treatment of myocardial ischemia. Conclusion:The effective substances in Panax notoginseng are obtained by investigating the relationship between the spectrum and efficiency, and a new method for the evaluation of spectrum-activity relationship for Panax notoginseng is established. It can objectively reflect the inher-ent quality of the drug and provide a new strategy for the further research of traditional Chinese medicines.

Objective To investigate the metabolism change of intestinal flora due to chronic hypoxia in infants. Methods Ten infants with tetralogy of fallot were considered as the chronic hypoxia group,10 healthy infants were regarded as the control group. The urine concen-tration of hippurate in the morning with fasting was detected by 1 H nuclear magnetic resonance. Results The concentration of hippurate was decreased in hypoxia group compared with the control group,(47. 15 ± 32. 88) mg/L vs (346. 698 ± 13. 555) mg/L,with significant differ-ence,P=0. 002. Conclusion Chronic hypoxia alters metabolism of intestinal flora in infants.

OBJECTIVE To study the analytic method for assaying of glutaral concentration by enzyme-labeled device at one time.METHODS Controlled solution of glutaral and sample solution were taken out and kept until the room temperature.Optical density value was detected at 450 nm.Content of sample was calculated by comparison with the known concentration of glutaral.RESULTS In the range of 0.75-2.50%,concentration of glutaral had linear relationship with the optical density value.Reproductive test:relative average error was within 2%,RSD≤2.51%;interference test:oxidation-resistant-sodium nitrite could make the result negative error.0.1% Sodium nitrite could make the test result decrease by 1.5% relatively.CONCLUSIONS The method is simple,fast and convenient,with accuracy which has met the requirement of hospital infection monitoring and acceptable interfering range made by additives,which is highly practical.

Nanoknife (irreversible electroporation) has demonstrated to be a safe and effective approach to tumor ablation,and plays a prominent role in application of treatment of pancreatic carcinoma,specifically locally advanced pancreatic carcinoma (LAPC).The complicated parameters of Nanoknife comparatively is still difficult.The advantage,optimal selection,adequate parameters regarding Nanoknife were reviewed in this article.

Objective To investigate the safety and efficacy of treatment with CT-guided percutaneous irreversible electroporation (IRE) of locally advanced pancreatic cancer(LAPC). Methods Patients with unresectable radiographic stage ⅢLAPC were prospectively collected. Comprehensive blood and imaging (CT,MRI,PET-CT) baseline examinations were completed and analyzed preoperatively. Operations were performed under general anesthesia and the needles were inserted under the guidance of CT. Ablative parameters were altered depending on the tumor size, anatomical location, and the number of electrodes. Needle withdrawal combined segmental ablation was chosen and the active electrode exposure length was 1 cm. All the imaging and serological follow-up examinations were performed within 1 to 3 days after procedures, at day 7 postoperatively, 1 and 3 months postoperatively to evaluate the safety and efficacy of IRE(according to modified response evaluation criteria in solid tumours of WHO). Results All the procedures were completed successfully. Immediately postoperative CT and/or MRI images showed all the ablation areas were well-defined and presented low-density necrosis without enhancement. The CT scans at day 7 postoperatively showed all the 14 lesions were drastically necrotized and the surrounding tissues were unharmed. CT and/or MRI scans at 1 month postoperatively revealed hypodense or low signal ablation areas without enhancement and the peritumoral and involved vessels and pancreatic duct were undamaged. All the patients had a CA199 transient rising, but it decreased progressively within 3 days and then markedly decreased at 1 weak and 1 month postoperatively. The laboratory carcino-embryonic antigen was almost within normal lists. Peripancreatic exudation was noted in 3 cases immediately after the procedures. One patient developed retroperitoneal infection. Mesenteric(2 of 4)and portal(2 of 4)venous thrombosis were found on MRI images in 4 patients. One patient had a duodenum stenosis and 1 died from serious gastrointestinal bleeding 2.5 months after IRE(unclear etiopathogenisis). The postoperative 3 months dynamic imaging showed that 4 of 13 reached complete response (CR) ,7 of 13 reached partial response (PR) and 2 of 13 had stable disease (SD). Conclusion CT-guided percutaneous IRE is safe and effective in patients with locally advanced pancreatic cancer.

PurposePifrrmann scoring based on T2WI can only evaluate intervertebral disc qualitatively and is easily affected by the surrounding environment and imaging parameters. The present study attempted to make a quantificational study on lumbar intervertebral disc degeneration by using MR T2 mapping sequence so as to improve its diagnosis.Materials and Methods Sagittal T1FSE, sagittal and axial T2FSE, sagittal T2 mapping sequence were performed on 265 lumbar intervertebral discs from 53 subjects. The MR imaging was analyzed and all the discs were classified according to Pifrrmann scoring. T2 relaxation time of the discs was calculated and statistical analysis was conducted on its correlation.Results Among the 265 discs, 5 (1.9%) were scored as Pfirrmann I, 153 (57.7%) were scored as Pfirrmann II, 86 (32.3%) were scored as Pfirrmann III, 20 (7.5%) were scored as Pfirrmann IV, 1 (0.38%) were scored as Pifrrmann V. The average anterior annulus T2 values showed no difference between every Pfirrmann grade (P>0.05), while the difference of average posterior annulus T2 values was significant between groups (P<0.05). The relaxation time of anterior annulus was signiifcantly shorter than that of posterior annulus in the discs of Pifrrmann II-IV, whilst the reverse occurred in the discs of Pifrrmann I (P<0.05). The differences of the relaxation time of nucleus pulposus T2 values were signiifcant between groups of Pifrrmann grade I to IV (P<0.05). The T2 relaxation time of each partition of nucleus pulposus (ROI2/ROI3, ROI3/ROI4, ROI2/ROI4) was signiifcantly correlated (r=0.86, 0.76 and 0.63,P<0.05), the T2 relaxation time of anterior annulus and posterior annulus had no correlation (r=0.09, P<0.05), and the nucleus pulposus T2 values and Pfirrmann grade were negatively correlated (r=-0.78,P<0.05).Conclusion MR T2 mapping can quantitatively evaluate the T2 relaxation time of lumbar intervertebral discs, and is signiifcantly correlated with Pifrrmann grades.

Objective To study the changes of posterior corneal elevation with one-year follow-up by using Allegro Oculyzer anterior segment diagnostic system in patients who had undergone laser in situ keratomileusis with femtosecond laser (FS-LASIK) for myopia. Methods Ninety eyes of forty-five patients who had undergone FS-LASIK for myopia were included in our study . The spherical equivalent of ametropia was-10.63D to -1.63D. The preoperative and postoperative corneal tomography (including 1, 3, 6 and 12 month after operation ) were collected by Allegro Oculyzer in the posterior corneal elevation and the thickness at central corneal thinnest point of each eye were measured , and the differences of the posterior corneal elevation were calculated. The data were analyzed with ANOVA and Pearson correlation analysis. Results There were no statistically significant differences in the changes of posterior corneal elevation from the preoperative time to the any postoperative follow-up time (F = 1.50, P > 0.05). There were no statistically significant changes of the posterior corneal elevation from the 1st month to 12th month postoperatively (F = 1.47, P > 0.05). There were statistically significant differences in the changes of thickness at central corneal thinnest point from the preoperative to the 12th month postoperative (F = 369.10, P <0.01). At postoperative time, the posterior corneal elevation was not correlated with several factors including spherical equivalent , ablation depth , residual bed thickness, ablation percentage per total corneal thickness and (corneal flap thickness + ablation depth) /thinnest point preoperative corneal thickness (P > 0.05). Conclusion After strictly followed surgical indications in FS-LASIK, the posterior corneal elevation can keep good stability and has no significant change. Thickness of central cornea thinnest point increases gradually and tends to stablity at the 3th months after surgery.

Objective To determine the complete nucleotide sequence of a genotype D hepatitis B virus (HBV) strain from a Uighur patient with chronic hepatitis B in Xinjiang.Methods The complete nucleotide sequence of a HBV strain obtained from a Uighur patient with chronic hepatitis B was amplified by polymerase chain reaction (PCR) and cloned for sequencing.The bioinformatics analysis was done using BioEdit and National Center for Biotechnology Information Basic Local Alignment Search Tool (NCBI-BLAST).Results The complete nucleotide sequence of Xinjiang HBV strain was 3174 bp.The nucleotide homology was 92%-98% between the Xinjiang strain and the published genotype D HBV strains and 91% compared to the published genotype D/C combination HBV strains from China (AY862865).There was a 9 bp deletion from nucleotide 1760 to nucleotide 1768 (ATTAAAGGT).This Xinjiang HBV strain belonged to ayw2 subtype and D genotype without any recombination with other genotypes.In the term of evolutionary relation,the complete nucleotide sequence of this Xinjiang HBV strain was closest to those of two Turkey HBV strains (AY796032 and AY721605) among 34 genotype D strains published in GenBank.Conclusions The complete nucleotide sequence of a genotype D HBV strain from a Uighur chronic hepatitis B patient in Xinjiang is determined.The sequence shows some unique characteristics.