Traditional Chinese medicine (TCM) with the characteristics of holistic view and treatment based on syndrome differentiation, has rich clinical experience thousands of years and demonstrates promising effects to cure complex disease. However, due to the features of multi-component, multi-target and synergistic effect existed in TCM, the effective substances and mechanisms of action are not clear, the qualities of TCM are out of control, and scientific and correct assess system is waiting to be established. The network pharmacology is a novel subject based on the construction of multi-layer networks of disease-phenotype-gene-drug to predict the drug targets in a holistic view, and promote efficiency of drug discovery. Methodologically, network pharmacology integrated the notions of comprehensive research and systematic assessment which agree with the characteristics of holistic view and treatment based on syndrome differentiation in Chinese medicine. Our paper reviewed the challenge and chance within the modernization of TCM, the concept and technology of network pharmacology, and its preliminary application in investigation of TCM. The theoretical system of network pharmacology is emphasized, and the potential prospect of its application in modernization in TCM is focused.

Aim To develop a sensitive, rapid and ac-curate LC-MSn method for determination of midazolam/1′-hydroxymidazolam and their pharmacokinetic char-acteristics in rat brain. Methods SD rats received in-travenous injection of midazolam ( 5 mg · kg-1 ) via femoral vein, a probe drug of cytochrome P450 3A. The microdialysis ( MD ) samples in situ brain were collected every 8 mins at 2. 0μl·min-1 in 2. 4 hours. Analytes in brain dialysate were quantified by the pro-posed LC-MSn method. Gradient elution was performed on an Agilent Eclipse Plus-C18 column ( 2 . 1 × 50 mm, 3. 5 μm). The mobile phase consisted of 2 mmol ·L-1 ammonium acetate and acetonitrile. The analyte was detected using electrospray ionization ( ESI ) in multiple reaction monitoring ( MRM) modes. The reac-tion selected ions were 326 . 1/291 . 1 m/z for midazo-lam, 342. 1/324. 1 m/z for 1′-hydroxymidazolam and 285. 1/154. 0 m/z for diazepam as internal standard. Result The linear ranges of midazolam and 1′-hydroxymidazolam were 0 . 78~100 and 0 . 195 ~12 . 5μg·L-1 respectively. The lower limit of quantification was 0 . 2 μg · L-1 . The RSD of intra- and inter-batch precisions was less than 7 %. The RSD of accuracy was from -1 . 34 to -8 %. Conclusion This sensi-tive and rapid LC-MSn method is suitable for determi-nation of midazolam/1′-hydroxymidazolam in rat brain dialysate. MD combined with LC-MSn method may give assistance to deep and further studies of drug metabo-lism and CYP3A enzyme in brain.

BACKGROUND: The sucrose is an ideal material for auxiliary support and mold fil ing that is suitable for application in the medical field with three-dimensional (3D) printing. In China, there are many studies about the 3D printing with sucrose, but the accuracy and porosity of the stent are stil unclear. OBJECTIVE: To explore the technological parameters of the sucrose scaffold by 3D printing technology. METHODS: From the perspective of physical and chemical properties, the sucrose viscosity and thermal decomposition with the change of temperature were analyzed. Based on the pneumatic controlled FDM technology, the mature sucrose scaffold was obtained by researching the match of temperature and pressure, layer setting, as wel as the match of speed and pressure. Then the scaffold was demarcated with microscope, and the porosity was measured by immersion in absolute ethyl alcohol. RESULTS AND CONCLUSION: The sucrose was completely melted at 180 oC, with the biggest liquidity. When the temperature was over 195 oC, the caramel reaction occurred. As the temperature increased, the sucrose viscosity decreased. The optimal molding parameters of the sucrose scaffold with pneumatic control-based 3D printing were 170 oC-0.2 MPa-12 mm/s (temperature-pressure-printing speed). The line width and mean porosity of the wel -shaped sucrose scaffold were 700 μm and 81.893%, respectively.

Objectives:To explore the effects of love resources and the lovers'resources equivalence on their romantic relationship quality.Methods:A sample of 203 pairs of lovers from 5 provinces and municipalities were selected(aged from 15 to 29).They were assessed with the Evaluation Form of Love Resources for Individual(EFL-RI)and the Scale of Relationship Perception Index for Unmarried People(RPUS)for the evaluation of the relation-ship between the love resource equivalence and the romantic relationship quality.The EFLRI was used to quantify the 12 individual love resources (the appearance of individual,stature,sexual health,health,personality,mental health,sense of responsibility,capacity,professional/occupation,education background,earning and family back-ground).The results included self-evaluation and peer-evaluation.Results:Self-assessment of responsibility between lovers and D-value of mental health were negatively correlated with the reported RPUS scores of both sides (r =-0.20 --0.33,Ps <0.05).The D-values of earning and family background were positively correlated with the re-ported RPUS scores of both sides (r =0.14 -0.25,Ps <0.05).Lovers'peer-evaluation of sexual health scores and the D-values of responsibility were negatively correlated with the reported RPUS scores of both sides (r =-0.25 --0.38,Ps <0.01.The D-values of professional/occupation and family background were positively correlated with the reported RPUS scores of males (r =0.20,0.18,Ps <0.05).Love resources(responsibility,sexual health,educa-tion background of males;appearance,personality,capacity of females )had positive predictability to males'repor-ted RPUS scores (β=0.14 -0.34),love resources(responsibility,appearance,personality,capacity,health of males and health,education background of females )had positive predictability to females'reported RPUS scores (β=0.14 -0.27).Conclusion:It suggests that the equivalence of responsibility resource has a positive influence for the quality of love relationship.The equivalence of family background resource has a negative effect for the quality of love relationship.

Objective To screening up-regulated protein kinases and their inhibitors in order to provide new targets for molecular thera-py of nasopharyngeal cancer.Methods GEO database and SAM software were employed to screen the up-regulated protein kinase gene in nasopharyngeal cancer.Based on DAVID database,the regulating functions of kinases were identified.The inhibitors of up-regulated kinase genes were identified by Selleckchem database.Literature mining was used to screen the potential anti-cancer drugs.Results Totally 2360 differentially expressed genes including 21 up-regulated protein kinases (CHEK1,CHEK2,PRKDC,AURKA,VRK2,STK17A,MELK,NU-AK1,TRPM7,MASTL,AXL,BUB1,BUB1B,CDK4,TTK,CDC7,CASK,AKT3,TBK1 and PBK)were identified in the whole genome profi-ling (Fold Change≥2,P <0.05).The results of function analysis showed the up-regulated genes were enriched in 10 function terms such as‘protein amino acid phosphorylation’‘phosphorylation’‘phosphate metabolic process’‘mitotic cell cycle’‘cell cycle phase’‘regulation of cell cycle’,and so on.The Selleckchem database analysis showed there were 9 up-regulated protein kinases equipped with 51 inhibitors which were proved already.The results of literature mining showed that 18 inhibitors of them had a few studies (less than 10 literatures)in cancer terms,and there was a potential to become new drugs to treat nasopharyngeal cancer.Conclusion A total of 21 up-regulated protein kinases were identified,and they might promote the nasopharyngeal carcinoma by regulating functions such as the cell-cycle control pathway.Their ki-nase inhibitors may have a potential role in anti-cancer treatment,which provided a new target point for molecular therapy of nasopharyngeal cancer.

Objective:To establish a method for the determination of 9 residual solvents including methanol, ethanol, acetone, i-sopropyl alcohol, acetonitrile, dichloromethane, methyl-butyl ether, ethyl acetate and methylbenzene by capillary gas chromatography. Methods:The residual solvents were separated on a Kromat KB-624(30 m × 0. 53 mm,3. 0 μm) capillary chromatographic column with temperature programming. Nitrogen was used as the carrier gas with the flow rate of 5. 0 ml·min-1 . The temperature of injector was 200 ℃. A flame ionization detector was used with the temperature of 250℃. The containers of head-space injector were in equilib-rium at 80℃ for 20 min. Dimethyl sulfoxide was used as the solvent and an external standard method was used for the determination of the 9 residual solvents. The injection volume was 1 ml. Results:Under the above conditions, the 9 solvents were well separated. There was good linear relationship within the concentration range, and the correlation coefficients were no less than 0. 9991. The average re-coveries were 99. 08%-105. 14% with RSD of 1. 1%-3. 3%(n=9). The LOQ was 15. 68, 25. 45, 1. 09, 10. 61, 4. 54, 5. 73, 1. 04, 10. 58 and 1. 89 μg, respectively. Conclusion:The established method is simple, accurate and reproducible, and can be used in the determination of residual solvents in irbesartan API.

Objective To discuss the value of vacuum sealing drainage combined with continuous fluid irrigation in deep second degree burn.Methods 33 patients with deep second degree burns in burn department of the Third Affiliated Hospital of Qiqihar Medical College were selected as study object.The observation group used vacuum sealing drainage combined with continuous fluid irrigation,while the control group used the traditional method of treatment.The rate of wound healing,wound healing time and pain conditions were compared between the two groups of patients.Results After 3 days in the observation group,there was dissolved necrotic tissue which were drained into the negative pressure bottles,wound necrosis dropped off and dissolved when changing VSD dressings after seven days,a large number of newborn epithelial tissue appeared on the wound after 14 days of treatment.The healing rate after 7 days and 14 days,total healing time and wound pain showed statistically significant differences between two groups.Conclusions VSD combined with continuous fluid irrigation can effectively improve local microcirculation of burn wounds,increase local blood flow,keep the wound stay at a moist environment,accelerate the exudate drainage,which will help reduce the damage caused by local inflammation,promote epithelial cell regeneration from multiple perspectives,promote wound healing,and improve local pain symptoms and the quality of life of patients.

Objective To summarize the experience of l ap aroscopic pyloromyotomy for the treatment of infantile hypertrophic pyloric sten osis (IHPS). Methods Laparoscopic pyloromyotomy was employed i n 50 cases of IHPS from April 2001 to April 2004. The patients aged 12~90 days ( mean, 35 days). Three 3-mm-diameter trocars were introduced into the abdominal c avity via the left, right and upper abdominal wall, respectively. A non-traumati c grasping forceps was inserted through the left trocar to manipulate the gastri c wall near the pylorus. Through the right trocar, a retractable pyloromyotomy s calpel, a stripper and a dissecting forceps were in turn utilized to complete py loromyotomy. Results The operation was completed under laparos cope in 48 cases, while a conversion to open surgery was needed in 2 cases (1 c ase of prepyloric membrane and 1 case of pyloric mucosal injury, which were cure d by open repair). The operative time was 15~45 min (mean, 25 min). The nasogast ric tube was removed and oral feeding with milk was attempted 6 hours after the operation. The patients were discharged at 3~5 postoperative days. Follow-up for 3~6 months (mean, 4.5 months) in 42 cases showed that the patients were normall y developed. Conclusions Laparoscopic pyloromyotomy can be wel l performed in the presence of experience in open surgery, technical skills in l aparoscopy, effective anesthesia and proper surgical instruments.

Objective To summarize the value of laparoscopic operation using an ultrasonic scalpel for the radical treatment of Hischsprung’s disease (HD) in infants and children. Methods Laparoscopic operation was performed in 20 infants and children with HD under general anesthesia from February 2003 to January 2006. The CO_2 pressure of pneumoperitoneum was set at 10~12 mm Hg. By way of three abdominal ports, the diseased colon and rectum were mobilized by dissecting the supplying vessels and severing the peritoneal reflection in the pelvic floor. The modified Soave pull-through procedure was employed to incise and mobilize the rectal mucosa at 0.5 cm above the dentate line. The lateral ligament of rectum was severed and the intraabdominal cavity was reached through the peritoneal reflection. The diseased colon was pulled through the anus passing the level of the transition zone for resection. Then coloanal anastomosis was made between the proximal colon and the cutting edge of the anal canal above the dentate line. Results All the procedures were completed smoothly. The operation time was 120~210 min (mean, 132 min). The length of resected colon was 20~55 cm (mean, 35 cm). The intraoperative blood loss was 5~20 ml (mean, 14 ml), with no need of blood transfusion. Enterocolitis happened in 2 cases postoperatively. Follow-up observations in the 20 cases for 3~36 months (mean, 12.5 months) found no complications. The patients had 1~2 times of fecal discharge per day during follow-up. Conclusions Laparoscopic pull-through procedure using an ultrasonic surgical scalpel has advantages of minimal invasion, little hemorrhage, simple performance, quick recovery, and low complication rate.

Ischemic stroke, gravely affecting human health with its increasing incidence rate , is a common disease of old age . JAK/STAT way , a recently discovered signaling pathway , is not only widely involved in processes of cell growth ,differentiation, and apoptosis ,but also closely related to the pathophysiology of stroke.However,the pathway function and mechanism in ische-mic cerebral stroke has not yet been fully elucidated .We will review the role and mechanism of JAK/STAT signal transduction pathway in ischemic stroke ,and scientifically draw network chart between various related signal molecules in the process of ische-mic stroke neuropathy combining both domestic and foreign re-search reported in this paper , in order to better understand the pathological mechanism process of brain stroke , find new drugs for the treatment of cerebral ischemic diseases ,then provide more systematic scientific literature support .