Immunity reconstruction treatment using dendritic cells-cytokine induced killer cells (DC-CIK)not only is effective in killing cancer cells, but also enables a quick recovery of chemotherapy patients, thus has received more and more attention for its clinical use. Radix astragali polysaccharide, the extract of traditional Chinese medicine radix astragali, not only has an anti-tumor function, but also can enhance the immunity of cancer patients and reduce the side effects of the chemotherapy. Further researches are needed to validate the combined use of these two treatments in alleviating clinical symptoms of post-chemotherapy breast cancer patients.

Objective To investigate the relationship between polymorphisms in the thymidylate synthase(TS) promoter enhancer region and invasion of bladder transitional cell carcinoma(BTCC).Methods The TS gene promoter and TS protein expression were detected by PCR and immunohistochemical Streptavidin-Peroxidase staining method respectively in 87 cases of BTCC and the immunoreactivity score(IRS) was estimated.Results The IRS of TS in BTCC was(3.38?2.15).The IRS of TS protein in stage of T_(2-4) was significantly higher than that in stage of T_(a-1),and the IRS was elevated with the increase of pathological grade of BTCC(all P

Objective To study the interaction of genetic polymorphisms of CYP2E1 and environmental hazard factors in gastric cancer. Methods 1∶1 case-control study was carried out in Nanjing area, using PCR-RFLP technology to determined the genetic polymorphisms of CYP2E1 and epidemiological data about environmental exposure of 121 cases and controls were collected. Interaction indexes (?) were calculated to determine the type of gene-environment interaction. Results After confounding adjusted, the result showed that interaction existed in genetic polymorphisms of CYP2E1 RsaⅠand the family history of digestive system disease, smoking, pickled food, irregular diet habit, interaction indexes (?) value were 1.855, 2.626, 1.736 and 1.714 respectively. A low exposure-gene effect interaction was found in CYP2E1 RsaⅠgenotype and the frequency of pickled food consumed, while a high exposure-gene effect interaction exist in CYP2E1 RsaⅠgenotype and irregular diet habit. Conclusion Genetic and environmental hazard factors co-effect the development of gastric cancer, genetic polymorphisms of CYP2E1 and environmental risk factors show an interaction in gastric cancer.

Objective To investigate the effects of the transfection of DNMT1 antisense oligonucleotide wrapped in liposome on the promotor methylation and expression of progesterone receptor in leukemia cell lines so as to find a new pathway of methylation for gene therapy. Methods Artificially synthesized DNMT1 antisense oligonucleotide MG88 wrapped in liposome was transfected into the leukemia cell line. Changes of DNMT1 & PRB mRNA were detected by RT PCR, and the changes of the methylation status of PRA & PRB were analyzed by MSP. Results After transfection of leukemia cell line with MG88, DNMT1 mRNA decreased significantly. PRA & PRB promotors were demethylated, and the expressions of PRB mRNA increased significantly. Conclusion MG88 can induce the demethylation of PR promoters in leukemia cell line, resulting in the increase of PRB mRNA expression. So MG88 can be used in the experimental study of DNA methylation for gene therapy.

Objective To investigate the drug resistance and the clinical distribution of nonfer-menters in the recent three years in our hospital, and to provide basis for rational use of antibacterials.Methods The bacteria were identified by VITEK32 bacteria identification system and Kirby-Bauer(K-B) method was used for the antibiotics susceptibility test. The data was statistically analyzed. Results A total of 747 strains of nonfermenters were isolated from various clinical specimens during Jan 2005 to Dec 2007. The most common nonfermenters were Pseudomonas aeruginosa, followed by Acineto-bacter baumannii, Stenotrophomonas maltophilia and Acinetobacter lowfii. The isolation rate of non-fermenters was the highest from sputum specimens(59.3 %). These bacteria had various resistances to all detected antibiotics. These nonfermenters were lowly resistant to imipenem and piperaeillin/tazo-bacam. The resistance rate of Stenotrophomonas maltophilia to minocycline, imipenem and levofloxa-cin was 1.2%, 3. 45 and 10. 3% respectively. Conclusion The nonfermenters are main pathogenic bacteria of nosoeomial infection, they are isolated from different clinical specimens and their drug-re-sistance is variant and multiple. To reduce-the production of drug-resistance and control the nosocomi-cal infection, the specimens must be collected and detected timely; the antibiotics should be selected according to the results of susceptibility test.

ObjectiveTo evaluate the application value of the emergency laparoscopic surgery in incarcerated inguinal hernia of children. MethodsManual reduction of incarcerated hernia was laparoscopic- assisted after anesthesia, high-ligation of hernia sac was done by one hole method of laparoscopic treatment.ResultsAll of 22 cases of pediatric incarcerated hernia were done, no complications occurred. Eighteen patients were followed up for 3 - 15 months, 9.3 months on average, without serious complications. ConclusionsThe emergency laparoscopic surgery in children of incarcerated inguinal hernia is safe and feasible,with smaller trauma, quicker recovery and smaller health risk.

Objective To study a new procedure of surgical repairment of rectourethral fistula.Method By the perineum approach one stage urethroplasty using island skin tubes from perineum and scrotum has been adopted after the resection of fistular urethra and repairment of the defect of the anterior wall of rectum to substitute the defect of urethra in 9 cases during 1992-1998.Result The 9 cases were all cured and have been followed-up for 2 6 years without recurrence of rectourethral fistula.Conclusion This procedure was regarded as an ideal technique for the closure of rectourethral fistula especially for those complicated with urethra stricture.

Objective To investigate the inductive role of traumatic microenvironment in dedifferentiation of epidermal cells and explore the potential role of Wnt signaling pathway in this biological process. Methods The sheets of human foreskin were digested overnight after removal of adipose tissue, and then the epidermis was separated from the dermis. The separated epidermis sheets were repeatedly adhered to type Ⅳ collagen and flushed to remove the epidermal stem cells. The obtained epidermis sheets were transplanted onto the full-thickness skin wounds on the back of BALB/c nude mice, five days after which the cell lineage was evaluated by immunohistochemistry and the expressions of Wnts and downstream components in the grafted epidermal sheets examined by RT-PCR and Western blot. Results The cells in the basal layer of full-thickness epidermal sheets were positive for CK19 and β1 integrin and negative for CK10. While the cells in uhrathin epidermal sheets treated with type Ⅳ collagen were fully positive for CK10. Five days after transplantation of the ultrathin epidermal sheets, cells negative for CK10 but positive for CK19 and β1 integrin emerged at the wound-neighboring side of the skin grafts. At the same time, the expressions of Wnt-10b, Wnt-4 and Wnt-7a mRNA were increased by about 3.1-fold, 2.2-fold and 1.4-fold independently after transplantation. Furthermore, the expressions of β-catenin and β-catenin target genes (cyclin D1 and c-myc) were elevated by about 3-fold, 1.5-fold and 2-fold respectively in the grafted epidermal sheets (P < 0.01). Conclusion Traumatic microenvironment can induce epidermal cell dedifferentiation, when the Wnt/β -catenin signaling pathway may play an important role.

OBJECTIVE To explore the changes in foot fungus mobility and its drug resistance for further etiology investigation and clinical treatment. METHODS Sabourand′s agar culture medium was used to culture fungi, ID identification strip was employed to identify the fungi and drug sensitive test was performed by disk diffusion test. RESULTS The incidence of Trichophyton rubrum infection was the highest (79.1%). The isolated fungi were relatively sensitive to amphotericin B (AMB, 98.9%) and itraconazole (ITC, 98.0%), and resistant to 5-fluorocytosine (5-FC, 22.1%). CONCLUSIONS Detection technique of fungal infection should be improved and anti-fungal medicine should be used reasonably according to the results of drug sensitive test so that the fungal infection, especially fungi-resistant infection could be reduced.

OBJECTIVE To monitor the characteristics of distribution and drug resistance of Acinetobacter baumannii in our hospital. METHODS A. baumannii isolates were collected in our hospital from Jan 2004 to Dec 2005. Antimicrobial susceptibility testing was performed by disk-diffusion method,according to the standards of NCCLS 2004. RESULTS Totally 177 strains of A. baumannii were distributed clinically in the respiratory unit as the most ones (47 strains, 26.6%), and in ICU as the next (38 strains, 21.5%); the older the age, the higher the appearing rate; the highest appearing rate was from the sputum, up to 78.1%; more than 60% of isolates were resistant to all antimicrobial agents tested except imipenem, meropenem and cefoperazone/sulbactam. However,10 pan-resistant strains were found. CONCLUSIONS With the increasing isolation rate of A. baumannii, its drug resistance increases simultaneously.