Immunity reconstruction treatment using dendritic cells-cytokine induced killer cells (DC-CIK)not only is effective in killing cancer cells, but also enables a quick recovery of chemotherapy patients, thus has received more and more attention for its clinical use. Radix astragali polysaccharide, the extract of traditional Chinese medicine radix astragali, not only has an anti-tumor function, but also can enhance the immunity of cancer patients and reduce the side effects of the chemotherapy. Further researches are needed to validate the combined use of these two treatments in alleviating clinical symptoms of post-chemotherapy breast cancer patients.

Objective To study the interaction of genetic polymorphisms of CYP2E1 and environmental hazard factors in gastric cancer. Methods 1∶1 case-control study was carried out in Nanjing area, using PCR-RFLP technology to determined the genetic polymorphisms of CYP2E1 and epidemiological data about environmental exposure of 121 cases and controls were collected. Interaction indexes (?) were calculated to determine the type of gene-environment interaction. Results After confounding adjusted, the result showed that interaction existed in genetic polymorphisms of CYP2E1 RsaⅠand the family history of digestive system disease, smoking, pickled food, irregular diet habit, interaction indexes (?) value were 1.855, 2.626, 1.736 and 1.714 respectively. A low exposure-gene effect interaction was found in CYP2E1 RsaⅠgenotype and the frequency of pickled food consumed, while a high exposure-gene effect interaction exist in CYP2E1 RsaⅠgenotype and irregular diet habit. Conclusion Genetic and environmental hazard factors co-effect the development of gastric cancer, genetic polymorphisms of CYP2E1 and environmental risk factors show an interaction in gastric cancer.

Objective To investigate the relationship between polymorphisms in the thymidylate synthase(TS) promoter enhancer region and invasion of bladder transitional cell carcinoma(BTCC).Methods The TS gene promoter and TS protein expression were detected by PCR and immunohistochemical Streptavidin-Peroxidase staining method respectively in 87 cases of BTCC and the immunoreactivity score(IRS) was estimated.Results The IRS of TS in BTCC was(3.38?2.15).The IRS of TS protein in stage of T_(2-4) was significantly higher than that in stage of T_(a-1),and the IRS was elevated with the increase of pathological grade of BTCC(all P

Objective To investigate the effects of the transfection of DNMT1 antisense oligonucleotide wrapped in liposome on the promotor methylation and expression of progesterone receptor in leukemia cell lines so as to find a new pathway of methylation for gene therapy. Methods Artificially synthesized DNMT1 antisense oligonucleotide MG88 wrapped in liposome was transfected into the leukemia cell line. Changes of DNMT1 & PRB mRNA were detected by RT PCR, and the changes of the methylation status of PRA & PRB were analyzed by MSP. Results After transfection of leukemia cell line with MG88, DNMT1 mRNA decreased significantly. PRA & PRB promotors were demethylated, and the expressions of PRB mRNA increased significantly. Conclusion MG88 can induce the demethylation of PR promoters in leukemia cell line, resulting in the increase of PRB mRNA expression. So MG88 can be used in the experimental study of DNA methylation for gene therapy.

Objective To investigate the drug resistance and the clinical distribution of nonfer-menters in the recent three years in our hospital, and to provide basis for rational use of antibacterials.Methods The bacteria were identified by VITEK32 bacteria identification system and Kirby-Bauer(K-B) method was used for the antibiotics susceptibility test. The data was statistically analyzed. Results A total of 747 strains of nonfermenters were isolated from various clinical specimens during Jan 2005 to Dec 2007. The most common nonfermenters were Pseudomonas aeruginosa, followed by Acineto-bacter baumannii, Stenotrophomonas maltophilia and Acinetobacter lowfii. The isolation rate of non-fermenters was the highest from sputum specimens(59.3 %). These bacteria had various resistances to all detected antibiotics. These nonfermenters were lowly resistant to imipenem and piperaeillin/tazo-bacam. The resistance rate of Stenotrophomonas maltophilia to minocycline, imipenem and levofloxa-cin was 1.2%, 3. 45 and 10. 3% respectively. Conclusion The nonfermenters are main pathogenic bacteria of nosoeomial infection, they are isolated from different clinical specimens and their drug-re-sistance is variant and multiple. To reduce-the production of drug-resistance and control the nosocomi-cal infection, the specimens must be collected and detected timely; the antibiotics should be selected according to the results of susceptibility test.

ObjectiveTo evaluate the application value of the emergency laparoscopic surgery in incarcerated inguinal hernia of children. MethodsManual reduction of incarcerated hernia was laparoscopic- assisted after anesthesia, high-ligation of hernia sac was done by one hole method of laparoscopic treatment.ResultsAll of 22 cases of pediatric incarcerated hernia were done, no complications occurred. Eighteen patients were followed up for 3 - 15 months, 9.3 months on average, without serious complications. ConclusionsThe emergency laparoscopic surgery in children of incarcerated inguinal hernia is safe and feasible,with smaller trauma, quicker recovery and smaller health risk.

Objective To explore the relevance among HER-2 gene amplification,microvessel density(MVD),tumor size,lymph node metastasis and histological grading,as well as to discuss the significance of the levels of HER-2 gene amplification and MVD on the prognostic judgment of breast cancer.Method From 2001 to 2005,30 breast cancer specimens were collected from the Affiliated Hospital of Academy of Military Medical Sciences.Coloring in situ hybridization(CISH) was used to examine HER-2 gene amplification in paraffin-embedded tissue samples.Microvessels in tissue were labeled by CD34 antibody using IHC,and the MVD was then counted under optical microscope.Result HER-2 gene amplification was found in 11 of 30 cases(36.7%).MVD in breast cancer samples with HER-2 gene amplification was 67.91?24.36,significantly higher than those samples without HER-2 gene amplification(47.48?13.27,P

Objective To probe into the characteristics of men who have sex with men living in small-medium sized cities and provide scientific foundation for the formulation of strategy for AIDS prevention and control.Methods A qualitative survey was carried out by convenience sampling for thirty one selected MSM.Results Interviewees had a large age gap,widely different occupations and various degrees of education;they depended mainly on internet and friends' contacts to find their sex partners;their knowledge about HIV/STDs was poor and they were indifferent to the risk of AIDs,but were faced with enormous psychological pressure.Multiple sex partners,unprotected anal intercourse were their main high risk behaviors,and they hoped to have access to free condoms,free HIV detection and hot line consultation.Conclusion To implement prevention in an effective manner,close attention should be paid to the role of key persons within the MSM group,to increasing knowledge of the target population,to protecting their mental health and to creating a friendly social environment for their existence and effective use of internet.

Objective To compare the optical properties and visual quality between Tecnis ZMB00 and AcrySof ReSTOR SN6AD1 multifocal intraocular lens (MIOL) in patients undergoing cataract surgery.Methods A total of 120 patients (130 eyes) who umderwent elective cataract surgery in our hospital from March 2012 to July 2015 were selected prospectively.All patients were treated with diffractive MIOL,and divided into group A (Tecnis ZMB00 group) and group B (AcrySof ReSTOR SN6AD1 group).The uncorrected distance visual acuity (UCDVA),uncorrected near visual acuity (UCNVA),uncorrected medium distance visual acuity (UCIVA),best corrected distance visual acuity (BCDVA) and best corrected near visual acuity (BCNVA) were measured before and after surgery.The defocusing curve was drawn for analysis of the contrast sensitivity,and the postoperative visual function and survival quality questionnaire (VF-14) was used to evaluate the postoperative visual quality.Results At 1 week,1 month and 3 months after surgery,recovery of UCNVA,UCIVA and BCNVA in group A was better than that in group B (all P ＜ 0.05).At 1 week and 3 months after surgery,the recovery of UCDVA in group A was better than that in group B (all P ＜ 0.05).At 3 months after surgery,the spherical equivalent was lower in group A than group B (P ＜ 0.05).The contrast sensitivities under scotopia,photopic glare and scotopic glare of different frequencies in group A were higher than those in group B (all P＜0.05).The VF-14 score in group A at 3 months after surgery was lower than that in group B (P＜0.05).In group A,0.0 D and-2.5 D formed 2 peaks,and from-2.0 D to-2.5 D formed the platform stage,while in group B,only 0.0 D and-3.0 D formed 2 peaks.Conclusion The implantation of Tecnis ZMB00 MIOL after cataract surgery can improve the uncorrected visual acuity of treated eye,contrast sensitivities,postoperative visual function and quality of life of patients.

Objective To observe the correlation between lipoprotein-associated phospholipase A2(Lp-PLA2) levels and coronary artery disease (CAD), extent of angiographic coronary artery lesions and risk factors of CAD in the elderly. Methods Plasma levels of Lp-PLA2, triglyceride (TG), total cholesterol(TC), low density lipoprotein(LDL-C), high density lipoprotein cholesterol (HDL-C) and high sensitivity C-reactive protein(hs-CRP) were measured in 67 elderly patients with angiographic CAD meanwhile in 23 normal controls without angiographic coronary artery lesions. The extent of coronary artery lesions was evaluated according to the number of vessel lesions (divided into single, double and triple-vessel lesions) and Gensini scoring system. Then the relationship between Lp-PLA2 and CAD was assessed. Results The plasma levels of Lp-PLA2 were significantly higher in the CAD group than in the controls [(352.7 ± 129.0) vs. (204.0 ± 59. 7) μg/L, P < 0. 01]. Lp-PLA2 levels increased with the number of coronary artery lesions and Gensini score, then were positively correlated with age(r= 0. 25, P<0. 05) ,TC(r=0. 33, P<0. 01) ,LDL-C(r=0.27, P< 0. 05),apoB(r=0. 36, P<0. 01). The levels of LP-PLA2 and LP(a) were associated with CAD by using stepwise regression analysis. Conclusions In the eldly, the levels of LP-PLA2 are much higher in angiographic CAD, and these may reflect the severity of CAD. LP-PLA2 is a risk factor for CAD.