Objective By systematically analyse from 1950 to 2013 Chinese medicine nursing research situation,the paper aimed to give a comprehensive insight into the current situation of the development in Traditional Chinese Medicine (TCM) nursing,speed up the nursing literature resources development and utilization of traditional Chinese medicine and promote the comprehensive development of the discipline.Methods Using China National Knowledge Infrastructure (CNKI) as the data resource,the method of literature metrology was adopted to retrieve the valid papers,including published time distribution,regional distribution,the dispatch of productive organization,issuing system of institution,different number distribution of authors,the author fund of cooperative research situation,the papers published periodical distribution,the cited frequency content.Results A total of 3 569 effective papers in this study were selected,of which papers from 2010 to 2013 accounted for 46.70％.19 institutions had the highest paper production,dispatch agencies belonged to different systems,among whichhospital and school accounted for a total amount of 96.30％,the core authors were 38 in the discipline research field,they published a total of 194 articles,but the two literature metrology index analysis of cooperation degreeand co-author rate showed that the authors lacked overall cooperation in the field of research.Conclusions Although the core authors had formed in the field of study,but through the analysis of parrot card law,the subject has not yet to reach maturity that the subject of the author's ideal distribution state.In scientific research activities for the future,researchers should strengthen cooperation,seek more high-level papers funded in order to enhance the development of TCM nursing level.Nurse practitioners of traditional Chinese medicine and related scientific research personnel should pay attention to related academic papers from areas prone to paper and paper productive organization.Relevant academic journals,especially the core journals in the field of research should increase Chinese medicine nursing papers from low level hospitals and basic medical service organization.At the same time we look forward to professional nursing journal of traditional Chinese medicine being founded.

Immunity reconstruction treatment using dendritic cells-cytokine induced killer cells (DC-CIK)not only is effective in killing cancer cells, but also enables a quick recovery of chemotherapy patients, thus has received more and more attention for its clinical use. Radix astragali polysaccharide, the extract of traditional Chinese medicine radix astragali, not only has an anti-tumor function, but also can enhance the immunity of cancer patients and reduce the side effects of the chemotherapy. Further researches are needed to validate the combined use of these two treatments in alleviating clinical symptoms of post-chemotherapy breast cancer patients.

Objective To investigate the effects of the transfection of DNMT1 antisense oligonucleotide wrapped in liposome on the promotor methylation and expression of progesterone receptor in leukemia cell lines so as to find a new pathway of methylation for gene therapy. Methods Artificially synthesized DNMT1 antisense oligonucleotide MG88 wrapped in liposome was transfected into the leukemia cell line. Changes of DNMT1 & PRB mRNA were detected by RT PCR, and the changes of the methylation status of PRA & PRB were analyzed by MSP. Results After transfection of leukemia cell line with MG88, DNMT1 mRNA decreased significantly. PRA & PRB promotors were demethylated, and the expressions of PRB mRNA increased significantly. Conclusion MG88 can induce the demethylation of PR promoters in leukemia cell line, resulting in the increase of PRB mRNA expression. So MG88 can be used in the experimental study of DNA methylation for gene therapy.

Objective To evaluate the damaging degree of hepatic cell in the patients with hepatocirrhosis. Methods 30 cases of hepatocirrhosis and 15 cases of normal controls were performed the dynamic imaging with 99mTc-EHIDA. By the three compartmental model configurations, we acquired the time-activity curers of the liver cell,and calculated the function indexes liver cell extraction and excretion. Results The peak time and mean residence time in the hepatocirrhosis groups were higher than those in the normal controls(P

Objective To investigate the serum concentration of serum Fas(sFas) in patients with Graves' disease(GD) before and after ~(131)I therapy.Methods Serum levels of sFas were measured with enzyme linked-immuno-sorbent assay(ELISA) in 61 patients before and after ~(131)I therapy,and the results were compared with that the control subjects.Results The concentration of sFas was significantly higher in GD patients compared with that in the control subjects[(174.8?94.6)ng/L vs (96.6?10.2)ng/L,P0.05],and was significantly lower in patients with euthyroidism after 131 I therapy[(111.2?13.2)ng/L,P

Objective To investigate a antitumor effects of mouse original monoclonal antibody against hMIC -1 as intravenous administration with human pancreatic tumor in vivo and providing experimental data .Methods The fourty-eight mice were randomized into eight groups for loaded with two pancreatic tumor cell lines panc -1 or sw1990 respectively , and individual tumor growth was observed , antitumor efficacy was evaluated after using mouse original monoclonal antibody against hMIC-1 by intravenous administration .The pathological change with formalin fixed , paraffin embedded tissues section was viewed .Results There was a significant difference in tumor volume and weigt in intravenous injection of mouse original monoclonal antibody against hMIC-1 on load pancreatic tumor with nude mice group compared with that in the control group after four week treatment , and the mouse original monoclonal antibody against hMIC-1 demonstrated a close association between inhibition of tumor volume growth and dose-effective in the two xenograft models examined .Under examined microscope , the pancreatic tumor tissue was destroyed evidently in mouse original monoclonal antibody against hMIC-1 group.Conclusions The antitumor effect of intravenous injection for mouse original monoclonal antibody against hMIC-1 is better than that of systemic using gemcitabine .

Objective To evaluate the clinical efficacy of continuous irrigation with recombinant human acid fibroblast growth factor (rh-aFGF) combined with negative-pressure drainage in the treatment of refractory skin ulcer.Methods A clinical trial was performed from January 2010 to December 2012.Sixty patients with refractory skin ulcer were randomly and equally classified into three groups:group A receiving continuous irrigation with rhaFGF combined with negative-pressure drainage,group B receiving irrigation of raw surfaces with rh-aFGF followed by bandaging after debridement,group C receiving covering of raw surfaces with Vaseline gauze and absorbent gauze followed by bandaging after debridement.Dressings were changed once daily in every group.The treatment lasted 21 days.The efficacy of these regimens was evaluated on day 7,14 and 21.Results At the above time points for observation,both the granulation and epithelium tissues of ulcers grew well in group A,with a decrease in the volume of wounds and area of exposed deep tissues.There was a significant increase in the cure rate and reduction rate of wound volume in group A compared with the other two groups (all P ＜ 0.01).Conclusion Refractory dermal ulcer can be treated with continuous irrigation with rh-aFGF combined with negative-pressure drainage.

Objective To explore the relevance among HER-2 gene amplification,microvessel density(MVD),tumor size,lymph node metastasis and histological grading,as well as to discuss the significance of the levels of HER-2 gene amplification and MVD on the prognostic judgment of breast cancer.Method From 2001 to 2005,30 breast cancer specimens were collected from the Affiliated Hospital of Academy of Military Medical Sciences.Coloring in situ hybridization(CISH) was used to examine HER-2 gene amplification in paraffin-embedded tissue samples.Microvessels in tissue were labeled by CD34 antibody using IHC,and the MVD was then counted under optical microscope.Result HER-2 gene amplification was found in 11 of 30 cases(36.7%).MVD in breast cancer samples with HER-2 gene amplification was 67.91?24.36,significantly higher than those samples without HER-2 gene amplification(47.48?13.27,P

According to our previous experimental results, maltol is a powerful antioxidant against autoxidation and oxidative stress of eryth-rocytes. In this report, the toxicity and antioxidative action of maltol in mice was investigated. The results indicated that maltol was not toxic in mice when fed in a dose of 50mg/kg?d-1 (body weight) for 1 month. The antioxidative enzymes ( SOD and Cat ) activities and GSH content of erythrocytes were significantly increased. In addition, the lifespan of houseflies fed with maltol was prolonged.

Objective To investigate the feasibility of an ileal reservoir with self controllable urination. Methods A segment of ileum was under taken,the middle portion of which was made into a N shaped reservoir and the proximal portion used as the afferent limb.The distal ileum was pulled out and the two skin flaps were taken and encircled the efferent ileum to constitute the urinary outflow.A urinary controller similar to penile clamp was applied to control the urination with a balloon catheter. Results Ten dogs and five patients underwent this procedure and the patients were followed up for 3 to 14 months.Urodynamic examination showed that on the third month,the reservoir in animals reached a maximum capacity of (150?40)ml and a full filling pressure of (24.4?5.3)cmH 2O.The reservoir in the patients reached a maximum capacity of (290?80)ml,a full filling pressure of (36.3?8.2)cmH 2O,a maximum urinary flow rate of (20.3?4.7)ml/s with no residual urine.X ray examination showed good excretory function of the kidney without hydronephrosis.All patients can control urination themselves through the use of the urinary controller. Conclusions The new procedure has the advantages of self control lable of urination,simple surgical performance and few complications.