Objective To observe the effect of bone marrow mononuclear cells (BMMNCs) transplantation on high mobility group box1 (HMGB1) migration in stroke mouse. Methods BMMNCs were harvest and injected intravenously into stroke mouse model; modified neurological severity scores(mNSS) were tested; brain infarct vol-ume and related protein expression levels were measured, then HMGB1 migration were observed. Results mNSS score and brain infarct volume in transplantation group weresignificantly lower than vehicle group , while HMGB1 expression were significantly higher than vehicle group. The expression levels of RAGE and TNF-α in transplanta-tion group were significantly lower than vehicle group. Immunofluorescence assay showed that the release of HMGB1 in brain penumbra area of transplanted mouse was significantly less than vehicle groups. Conclusions BMMNCs transplantation could inhibit the release of HMGB1 in mouse brain.

Objective: To establish a HPLC method for the determination of liquiritin、 liquiritigenin and iso liquiritigenin in Licorice Mixture.Methods: A Cromasil C 18 column was used. the mobile phase was methanol 0.5% acetic acid for gradient elution. Liquiritin、 liquiritigenin and iso liquiritigenin were determined by dual wavelength, ? 1=276 nm,? 2=372 nm.Results: The linear range of liquiritin was 50.0～1250.0ng, r= 0.9999; The average recovery was 98.30%, RSD =1.01%( n= 6). The linear range of liquiritigenin was 5.0～125.0 ng, r= 0.9998; The average recovery was 98.01%, RSD =0.68%( n= 6). The linear range of iso liquiritigenin was 3.0～6.0 ng, r= 0.9999. The average recovery was 98.37%, RSD= 0.89%( n= 6). Conclusion: This method is convenient, rapid and accurate. It can be used for quality control in production of Licorice Mixture.

AIM:To establish an HPLC method for determining geniposide and liquiritin in Zhizi Baipi Decoction(Fructus Gardeniae,Cortex Phellodendri Chinensis,Radix et Rhizoma Glycyrrhizae). METHODS: A Kromasil C_(18) Column(4.6 mm?250 mm,5 ?m) was used.The mobile phase was methanol-0.5% glacial acetic acid for gradient elution.The flow rate was 1.0 mL/min,?_1=238 nm(detect the geniposide),?_2=276 nm(detect the liquiritin).The column temperature was at 30 ℃. RESULTS: The linear range of geniposide was within 49.1 ?g/mL-147.4 ?g/mL,r=(0.999 8),the recovery was(99.40%)(n=9).The linear range of liquiritin was within 6.1 ?g/mL-18.4 ?g/mL,r=(0.999 9),the recovery was 99.94%(n=9). CONCLUSION: This method has good reproducibility.It can be used for quality control in the production of Zhizi Baipi Decoction.

Objective To establish a quality control for Tianmatoufengling Capsule. Methods HPLC method was taken for the determination of Gastrodine in Tianmatoufengling Capsule,A Kromasil C18 column was used,with methanol 0.05% Calcium phosphace(2.5∶97.5) as the mobile phase,and its flow rate was 1.0 mL/min. The detection wavelength was 220 nm. TLC method was used for the identification of Tianmatoufengling Capsule. Results Under the used chromatographic condition,Tianmatoufengling had fine linear responses(r =0.999 9) in the concentration range of 6.016～96.256 ?g/mL,the average recovery was 100.02%,RSD was 2.24%. The method used in TLC-qualitative analysis was simple and sensitive with high specificity,four ingredients identified in Tianmatoufengling Capsule could be detected and all showed specific spots. Conclusion The method employed in this study was convenient and reliable with good repetition. It can be used for quality control in production of Tianmatoufengling Capsule.

AIM: To establish a HPLC for determination of puerarin and baicalin in Gegen Qinlian Micropill. METHODS: A Kromasil C 18 Column was used. The mobile phase was methanol-0.2% phosphoric acid. Puerarin and baicalin were determined by dual wavelength, ? 1=250nm. ? 2=315nm. RESULTS: The linear range of puerarin was within 32.0 ng—288.0 ng,r=0.9998 and the sample recovery was 100.17 % (RSD=1.00%(n=9)). The linear range of baicalin was within 52.6 ng～447.1 ng, r=0.9999 and the sample recovery was 100.06 % (RSD=1.06% (n=9)). CONCLUSION: This method has good repeatability and flexibility. It can be used for quality control of Gegen Qinlian Micropill.

AIM: To establish a HPLC for determination of baicalin and emodin in Yiqing Capsule(Radix Scutellarial, Radix et Rhizoma Rhei). METHODS:A Kromasil C_(18) Column was used. The mobile phase was methanol -0.2% phosphoric acid for gradient elution. Baicalin and emodin were determined by dual wavelength, ?_1=315 nm, ?_2=266 nm. RESULTS:The linear range of baicalin was within 64.40-322.00 ?g?mL~(-1) (r=(0.999 8)). The average recovery was 99.96%,RSD was 1.57%(n=9). The linear range of emodin was within( 0.82)-13.20 ?g?mL~(-1)(r=0.999 9).The average revovery was 98.90%, RSD was 1.25%(n=9). CONCLUSION: This method has good repeatability and flexibility. It can be used for quality control in production of Yiqing Capsule. (Nan

Objective To optimize the preparation process for Fangji Huangqi Granules. Methods With the extraction rates of tetrandrine, fanchinoline, astragaloside Ⅳand solid as the parameters, the extract conditions of Fangji Huangqi Granules were optimized by orthogonal design . Then the anti-inflammation effect of the extracts was observed on the mice and rats. Results The optimal preparation process was as follows:the mixture of medical materials was firstly refluxed twice with total 10 times of 70 %alcohol,1.5 hours for each time, and then extracted twice with total 12 times of boiling water ,1.5 hours for each time. The anti-inflammation effect of the extracts was obvious on the mice and rats. Conclusion The optimal preparation process is reasonable and with high extraction rate of active components.

Objective To establish a HPLC method for determination of hypoxanthine and xanthine in Syngnathus.Methods A Lichrosper C_(18) Column was used.The mobile phase was methanol-0.1%HAc.Walvelength was 254nm.Results The linear range of hypoxanthine was within 5.91～94.56?g?mL~(-1),r=0.9999,sample recovery rate was 99.22%,RSD=(1.25)%.The linear range of Xanthine was within 2.04~32.64?g?mL~(-1),r=0.9998,sample recovery rate was 98.05%,RSD=1.21%.Conclusion This method has good repeatability and flexibility.It can be used for quality control in production of Syngnathus.

AIM: To establish a HPLC method for determination of puerarin and paeoniflorin in Jingfukang Granules(Radix Puerariae, Radix Paeoniae Alba, etc.). METHODS: A Kromasil C 18 Column was used. The mobile phase was methanol water. Puerarin and paeoniflorin were determined by HPLC(dual wavelength, ? 1=250nm,? 2=230nm). RESULTS: The linear range of puerarin was within 99.6ng～996.0ng, r =0.9998, sample recovery was 100.69%, RSD =1.04%( n =9), respectively. The linear range of paeoniflorin was within 119.6ng～ 1315.6 ng, r =0.9999, sample recovery was 100.30%, RSD =1.24%( n =9), respectively. CONCLUSION: This method is simple, reliable and has good repeatability. It can be used for quality control of Jingfukang Granules in production.