Objective To study the clinical effect of Xingnaojing injection in treating patients with acute cer-ebral infarction and its influence on serum matrix metalloproteinase 9 (MMP -9),nitric oxide (NO)and nitric oxide synthase (NOS).Methods 112 patients with acute cerebral infarction were randomly divided into two groups by ran-dom number table method,56 cases in each group.The control group received routine treatment for acute cerebral infarction.The observation group received routine treatment plus intravenous injection Xingnaojing.The changes of NIHSS score,motor function score and sensory function score in the two groups were compared before and after treat-ment.The serum levels of MMP -9,NOS and NO in the two groups were observed before and after treatment.Results The total effective rate of observation group was 82.14%,which was significantly higher than 64.29% in the control group (χ2 =4.553,P <0.05).After treatment,NIHSS scores of the observation group were significantly lower than the control group (t =5.082,5.522,6.253,all P <0.01 ).After treatment for 3 weeks,the motor function score (69.54 ±17.82)points,sensory function score (30.58 ±7.94)points of the observation group were significantly higher than (52.93 ±14.66)points and (23.08 ±6.31)points in the control group (t =5.387,5.534,all P <0.01).After treatment,the serum MMP -9,NOS,NO levels of the observation group were significantly lower than the control group (all P <0.01 ).Conclusion Xingnaojing injection in the treatment of acute cerebral infarction has good curative effect,it can promote the recovery of neurological function,improve motor function and sensory function, and by adjusting the MMP -9,NOS,NO levels to protect the nerve and blood vessel function.

Objective:To study the relationship among serum uric acid (SUA)level,brachial-ankle pulse wave veloci-ty (baPWV)and severity of coronary disease.Methods:A total of 121 inpatients,who hospitalized in our depart-ment of cardiology from Jan 2011 to Jul 2012,were enrolled.According to results of coronary angiography,they were divided into coronary heart disease (CHD)group (n=59)and normal control group (n=62),CHD group was further divided into single vessel coronary disease group (n=30,single vessel group),double-vessel coronary disease group (n=14,double-vessel group)and multi-vessel coronary disease group (n=15,multi-vessel group).SUA level and baPWV were compared among all groups,and their relationship with degree of coronary disease was analyzed. Results:SUA concentration gradually rose along with number of diseased coronary vessels increased,it's (349.26± 96.58)μmol/L,(400.37±70.96)μmol/L,(517.57±85.26)μmol/L and (602.60±77.03)μmol/L in normal con-trol,single vessel,double-vessel and multi-vessel group respectively,P<0.05 or <0.01;baPWV gradually rose a-long with number of diseased coronary vessels increased,compared with normal control group,there was significant increase in baPWV [(1499.04±193.82)cm/s vs.(1885.32±319.73)cm/s,(2036.00±406.40)cm/s,(2171.03± 348.53)cm/s]in single vessel,double-vessel and multi-vessel group,that of double-vessel group was significantly higher than that of single vessel group,P<0.05~<0.01.Logistic regression analysis indicated that SUA and baP-WV were independent risk factors for CHD morbidity (OR=13.011,14.008,P=0.000).Conclusion:Serum uric acid level and pulse wave velocity possess important clinical application value for predicting CHD and its severity.

Purpose:To look for an ideal screening method in vitro for establishing osteosarcoma cell sublines with different metastat ic potential. Methods:20 osteosarcoma cell sublines were isolated preliminari ly by clone technique in vitro. They were screened by electrophores migratio n rate assay and cell migration assay in vitro and obtained respectively a h igh and low metastatic potential osteosarcoma cell sublines (A1、A2、B1 and B2). The advantages of the two methods were compared and confirmed by using cell pro liferation, agarose clony-formation and transplantation in nude mice. Results:The cell proliferation rate , agarose clony-formation ability and spontaneous metastatic ability to lung of A1 and B1 were obviously h igher than that of A2 and B2 and the difference was statistically significant( P

Objective To compare the optical properties and visual quality between Tecnis ZMB00 and AcrySof ReSTOR SN6AD1 multifocal intraocular lens (MIOL) in patients undergoing cataract surgery.Methods A total of 120 patients (130 eyes) who umderwent elective cataract surgery in our hospital from March 2012 to July 2015 were selected prospectively.All patients were treated with diffractive MIOL,and divided into group A (Tecnis ZMB00 group) and group B (AcrySof ReSTOR SN6AD1 group).The uncorrected distance visual acuity (UCDVA),uncorrected near visual acuity (UCNVA),uncorrected medium distance visual acuity (UCIVA),best corrected distance visual acuity (BCDVA) and best corrected near visual acuity (BCNVA) were measured before and after surgery.The defocusing curve was drawn for analysis of the contrast sensitivity,and the postoperative visual function and survival quality questionnaire (VF-14) was used to evaluate the postoperative visual quality.Results At 1 week,1 month and 3 months after surgery,recovery of UCNVA,UCIVA and BCNVA in group A was better than that in group B (all P ＜ 0.05).At 1 week and 3 months after surgery,the recovery of UCDVA in group A was better than that in group B (all P ＜ 0.05).At 3 months after surgery,the spherical equivalent was lower in group A than group B (P ＜ 0.05).The contrast sensitivities under scotopia,photopic glare and scotopic glare of different frequencies in group A were higher than those in group B (all P＜0.05).The VF-14 score in group A at 3 months after surgery was lower than that in group B (P＜0.05).In group A,0.0 D and-2.5 D formed 2 peaks,and from-2.0 D to-2.5 D formed the platform stage,while in group B,only 0.0 D and-3.0 D formed 2 peaks.Conclusion The implantation of Tecnis ZMB00 MIOL after cataract surgery can improve the uncorrected visual acuity of treated eye,contrast sensitivities,postoperative visual function and quality of life of patients.

Objective To investigate the effect of bone marrow mesenchymal stem cells (bone marrow stem cells,BMSCs) through different approaches in the treatment of transplanted cirrhotic model in rabbit effect difference.Methods Establishment of rabbit model of carbon tetrachloride cirrhosis with liquid olive oil mixture of subcutaneous inj ection and ethanol fed,the liver cirrho-sis models were randomly divided into cirrhosis model group (n=10),hepatic artery transplantation group (n=10),ear vein trans-plantation group (n=10).Healthy New Zealand rabbits bone marrow,using the method of whole bone marrow adherent cultured bone marrow BMSCs,and then were transplanted and serum albumin,alanine aminotransferase in 3 ways;by HE staining,the de-gree of hepatic cirrhosis and detection of serum albumin by Western blot expression in liver.Results Through the vein group and ar-tery transplantation group BMSCs to cirrhotic model in rabbit,4 weeks after the discovery of liver transplantation group rabbits were significantly improved,serum albumin is liver cirrhosis without transplantation group increased significantly(P<0.05),transami-nase is liver cirrhosis without transplantation group decreased significantly (P<0.05).The therapeutic effect of 2 ways of BMSCs transplantation on rabbit liver cirrhosis had a significant difference (P<0.05).Conclusion Transplantation of BMSCs through the ear vein and the hepatic artery of rabbit after 4 weeks of treatment of liver cirrhosis,liver function can be improved.But the hepatic artery transplantation therapy is obviously better than that of intravenous transplantation of treatment effect.

Chemotherapy is one of the main treatments for gastric cancer, but its drug resistance often limits the effectiveness of chemotherapy, leading to treatment failure. Drug resistance can be divided into primary drug resistance and secondary resistance. It has showed that several factors were involved in the multidrug resistance of gastric cancer, including the expression of drug resistance-related proteins, abnormalities of apoptosis-related genes, dysfunction of DNA damage repair, epithelial-mesenchymal transition and non-coding RNA. The solution to improve the efficacy of chemotherapy is to overcome drug resistance or delay drug resistance. This article will explore the progress in multi-drug resistance of gastric cancer.

Objective To explore the principle,effect and method of percutaneous AiDi injection for treatment of implanted VX2 liver tumor with ultrasound guidance.Methods Twenty-eight New Zealand white rabbits with implanted VX2 liver tumors were randomly divided into 3 groups:AiDi injection group (n=12) ,percutaneous ethanol injection (PEI) group (n=8) ,and physiological saline group (n=8) .Two weeks after transplantation,the drugs were injected into tumors of all the rabbits once every 3 days.After 4 times injection,the rabbits were killed.The tumors were measured with 64-slice CT,while the rate of tumor necrosis,the volume of tumor and the growth rate of the tumor were calculated.Liver and kidney function tests (AST,ALT,ALP and BUN) as well as white blood eell counts were assessed before and after treatment,and CT examination was performed at the same time.The morphological and the ultramicrostructural changes were also investigated under light microscope or electron microscope.Results Tumors in all groups kept growing after the procedure.Among all 3 groups,tumor growing rate for AiDi injection group was the lowest and the necrotic rate was the highest (P＜0.05) ,while no significant difference was found between AiDi injection group and PEI group (P＞0.05) .Apoptotic index in AiDi injection group was significantly higher than that in PEI group and physiological saline group (P＜0.05) .There was no evident change of liver and kidney function before and after treatment in AiDi injection group and PEI group (P＞0.05) .Conclusion AiDi injection can induce apoptosis of liver VX2 tumor cells.Percutaneous AiDi injection therapy can obviously inhibit and destroy liver VX2 tumor cells.

Objective To explore how different sex affect the clinical feature and apneic event,sleep time in patient with sleep apnea hypopnea syndrome.Methods According to the analysis of polysomnography(PSG),133 male and 47 women with chief complain of snoring were divided into four groups simple snor,mild SAS,moderate SAS and severe SAS group.Compared related clinical feature and PSG data among them ,we found the difference in it.Results Male patient have more complication than female patient (P

Objective To establish a quality control for Tianmatoufengling Capsule. Methods HPLC method was taken for the determination of Gastrodine in Tianmatoufengling Capsule,A Kromasil C18 column was used,with methanol 0.05% Calcium phosphace(2.5∶97.5) as the mobile phase,and its flow rate was 1.0 mL/min. The detection wavelength was 220 nm. TLC method was used for the identification of Tianmatoufengling Capsule. Results Under the used chromatographic condition,Tianmatoufengling had fine linear responses(r =0.999 9) in the concentration range of 6.016～96.256 ?g/mL,the average recovery was 100.02%,RSD was 2.24%. The method used in TLC-qualitative analysis was simple and sensitive with high specificity,four ingredients identified in Tianmatoufengling Capsule could be detected and all showed specific spots. Conclusion The method employed in this study was convenient and reliable with good repetition. It can be used for quality control in production of Tianmatoufengling Capsule.

Objective To study the chemical constituents in the seeds of Zizyphus jujuba var.spinosa.Methods The compounds were isolated by silica gel column chromatography and their structures were established by spectroscopic methods.Results A new keto-dammarane type saponin,jujuboside H(Ⅰ),along with three known compounds protojujuboside A(Ⅱ),spinosin(Ⅲ),and betulic acid(Ⅳ)were isolated.Conclusion Compound Ⅰ is a new compound named jujuboside H.