Objective:To study the relationship among serum uric acid (SUA)level,brachial-ankle pulse wave veloci-ty (baPWV)and severity of coronary disease.Methods:A total of 121 inpatients,who hospitalized in our depart-ment of cardiology from Jan 2011 to Jul 2012,were enrolled.According to results of coronary angiography,they were divided into coronary heart disease (CHD)group (n=59)and normal control group (n=62),CHD group was further divided into single vessel coronary disease group (n=30,single vessel group),double-vessel coronary disease group (n=14,double-vessel group)and multi-vessel coronary disease group (n=15,multi-vessel group).SUA level and baPWV were compared among all groups,and their relationship with degree of coronary disease was analyzed. Results:SUA concentration gradually rose along with number of diseased coronary vessels increased,it's (349.26± 96.58)μmol/L,(400.37±70.96)μmol/L,(517.57±85.26)μmol/L and (602.60±77.03)μmol/L in normal con-trol,single vessel,double-vessel and multi-vessel group respectively,P<0.05 or <0.01;baPWV gradually rose a-long with number of diseased coronary vessels increased,compared with normal control group,there was significant increase in baPWV [(1499.04±193.82)cm/s vs.(1885.32±319.73)cm/s,(2036.00±406.40)cm/s,(2171.03± 348.53)cm/s]in single vessel,double-vessel and multi-vessel group,that of double-vessel group was significantly higher than that of single vessel group,P<0.05~<0.01.Logistic regression analysis indicated that SUA and baP-WV were independent risk factors for CHD morbidity (OR=13.011,14.008,P=0.000).Conclusion:Serum uric acid level and pulse wave velocity possess important clinical application value for predicting CHD and its severity.

Objective To study the clinical effect of Xingnaojing injection in treating patients with acute cer-ebral infarction and its influence on serum matrix metalloproteinase 9 (MMP -9),nitric oxide (NO)and nitric oxide synthase (NOS).Methods 112 patients with acute cerebral infarction were randomly divided into two groups by ran-dom number table method,56 cases in each group.The control group received routine treatment for acute cerebral infarction.The observation group received routine treatment plus intravenous injection Xingnaojing.The changes of NIHSS score,motor function score and sensory function score in the two groups were compared before and after treat-ment.The serum levels of MMP -9,NOS and NO in the two groups were observed before and after treatment.Results The total effective rate of observation group was 82.14%,which was significantly higher than 64.29% in the control group (χ2 =4.553,P <0.05).After treatment,NIHSS scores of the observation group were significantly lower than the control group (t =5.082,5.522,6.253,all P <0.01 ).After treatment for 3 weeks,the motor function score (69.54 ±17.82)points,sensory function score (30.58 ±7.94)points of the observation group were significantly higher than (52.93 ±14.66)points and (23.08 ±6.31)points in the control group (t =5.387,5.534,all P <0.01).After treatment,the serum MMP -9,NOS,NO levels of the observation group were significantly lower than the control group (all P <0.01 ).Conclusion Xingnaojing injection in the treatment of acute cerebral infarction has good curative effect,it can promote the recovery of neurological function,improve motor function and sensory function, and by adjusting the MMP -9,NOS,NO levels to protect the nerve and blood vessel function.

Objective To explore the principle,effect and method of percutaneous AiDi injection for treatment of implanted VX2 liver tumor with ultrasound guidance.Methods Twenty-eight New Zealand white rabbits with implanted VX2 liver tumors were randomly divided into 3 groups:AiDi injection group (n=12) ,percutaneous ethanol injection (PEI) group (n=8) ,and physiological saline group (n=8) .Two weeks after transplantation,the drugs were injected into tumors of all the rabbits once every 3 days.After 4 times injection,the rabbits were killed.The tumors were measured with 64-slice CT,while the rate of tumor necrosis,the volume of tumor and the growth rate of the tumor were calculated.Liver and kidney function tests (AST,ALT,ALP and BUN) as well as white blood eell counts were assessed before and after treatment,and CT examination was performed at the same time.The morphological and the ultramicrostructural changes were also investigated under light microscope or electron microscope.Results Tumors in all groups kept growing after the procedure.Among all 3 groups,tumor growing rate for AiDi injection group was the lowest and the necrotic rate was the highest (P＜0.05) ,while no significant difference was found between AiDi injection group and PEI group (P＞0.05) .Apoptotic index in AiDi injection group was significantly higher than that in PEI group and physiological saline group (P＜0.05) .There was no evident change of liver and kidney function before and after treatment in AiDi injection group and PEI group (P＞0.05) .Conclusion AiDi injection can induce apoptosis of liver VX2 tumor cells.Percutaneous AiDi injection therapy can obviously inhibit and destroy liver VX2 tumor cells.

Objective To detect the methylation status of DLEC1 promoter in the tissue and serum of colorectal cancer(CRC) patients and to evaluate its clinical relevance.Methods Genomic DNA was extracted from the tissues(cancer tissue and corresponding adjacent normal tissue) and sera of 71 CRC patients;serum genomic DNA was also obtained from 20 patients with benign gastrointestinal diseases and 20 healthy donors.Promoter methylation status of DLEC1 gene was detected by methylation-specific polymerase chain reaction(MSP).Results The incidence of aberrant methylation of DLEC1 promoter was 45.1%(32/71) in CRC tissues,which was significantly higher than that in the adjacent normal tissues(7.1%,4/56)(P

Objective To explore how different sex affect the clinical feature and apneic event,sleep time in patient with sleep apnea hypopnea syndrome.Methods According to the analysis of polysomnography(PSG),133 male and 47 women with chief complain of snoring were divided into four groups simple snor,mild SAS,moderate SAS and severe SAS group.Compared related clinical feature and PSG data among them ,we found the difference in it.Results Male patient have more complication than female patient (P

Objective To investigate the long-term therapeutic effectiveness of transplantation of adrenals for the treatment of Addison's disease in the past 15 years. Methods Nine cases subject to adrenal transplantation were followed up for a long-term and the outcome after 15 years was evaluated. Results Seven cases of graft adrenal survived. In 6 patients, urine 17-OHCS, urine 17-KS and serum cortisol were normal. The remaining two grafts out of the 9 cases didn't survive and they had abnormal levels of urine 17-OHCS, urine 17-KS and serum cortisol. Conclusion The transplantation of adrenals for the treatment of Addison's disease had a good long-term effectiveness.

Objective The purpose of this study is to investigate the surgical procedures and curative effect through a small incision on both sides of the scapula for surgical resection the ribs tumor which located un -der ventral surface of the scapula .Methods The group included 11 patients,6 males and 5 females,aged 18 to 49 years.Lesions were bilateral scapula deep square 2~4 single rib bone tumors .Among them there were five ca-ses of fibrous dysplasia ,case of bone cyst ,2 cases of plasma cell tumors and metastatic tumors in 3 cases.Surgery incision through cross-scapular ,to determine which lesions ribs ,cutting the lesions on both sides of the stripping and junction.Results The group obtained a satisfactory excision extension ,one case appeared hemopneumotho-rax postoperatively.Secondly,none of patients occured local recurrence .Thirdly,two patients with metastatic carci-noma died after one year because of multiple metastases .Conclusion Straddling scapular notch is the ideal way to cut off the rib tumor of ventral surface of the scapula .Possessing advantages of a little damage and effect on the function,and rapid recovery .

Objective To analyze the effect of different procedures of alimentary reconstruction for the digestive and absorptive functions of patients after total gastrectomy. Methods From January 2012 to January 2013,there were 114 patients with gastric cancer,of which 58 patients received Roux-en-Y anastomosis were the control group,56 patients received P-type jejunal loop esophageal jejunal Roux-en-Y anas-tomosis were the observation group,the nutritional status and postoperative complications of two groups were compared. Results The level of RBC,HGB,TP and ALB of the observation group were significantly higher than that of the control group(P<0. 05),the incidence rate of in-traoperative complications,diarrhea and reflux esophagitis of the two groups had no significant differences (P>0. 05),but the incidence rate of postoperative dumping syndrome and abdominal distension of the observation group were significantly lower than that of the control group (P<0. 05). Conclusion The efficacy of P-type jejunal loop esophageal jejunal Roux-en-Y anastomosis for gastric surgery was superior to Roux-en-Y anastomosis.

Objective To study expression intensity and distribution of phosphorylated forms of extracellular signal-regulated protein kinase1/2 (ERK1/2), stress-activated protein kinase (SAPK) and P38MAPK in normal skin and hypertrophic scars and their potential biological significance. Methods The morphological characteristics of hypertrophic scars in different periods after wound healing and normal skin were examined histopathologically. Protein expression of p-ERK1/2, p-SAPK and p-P38MAPK was also assessed with immunohistochemical technique. Results In normal skin, phosphorylated forms of ERK1/2, SAPK and P38MAPK were mainly located in epidermal basal cells, in which the positive cellular rates of all the three proteins were low. Along with the maturation of hypertrophic scars, protein contents of p-ERK1/2 and p-SAPK were progressively increased. In mature hypertrophic scars, positive signals of these two proteins were mostly distributed in keratinocytes and some fibroblasts. The positive cellular rate of p-P38MAPK ascended in active hypertrophic scar, then decreased to a level which was still higher than that of normal skin. Conclusion The formation of hypertrophic scars may be associated with the alteration in signaling pathways which results in the increment of protein contents of p-ERK1/2, p-SAPK and p-P38MAPK.

Objective To investigate gene expression of epidermal growth factor (EGF), its receptor (EGFR) and two protooncogenes (c-fos and c-myc), in fetal skin at different development stages and children skin and to delineate their potential biological significance. Methods Fetal skin biopsies of human embryo were obtained from spontaneous abortions at different gestational ages from 13 to 32 weeks and children skin specimens were collected from patients undergoing plastic surgery. After morphological characteristics of skin at different development stages were detected with histological methods, gene expressions of EGF, EGFR, c-fos and c-myc in skin at different development stages were examined with reverse transcription-polymerase chain reaction analysis (RT-PCR). Results Gene expression of EGF, EGFR, c-fos or c-myc could all be detected in fetal and childern skins. In fetal skin, the gene expression of these 4 genes was weak. Gene expression of these genes in skin was progressively enhanced with increasing gestational age. In children skin, the mRNA contents of these 4 genes were significantly increased in comparison with those in fetal skin (P