Objective To study the clinical effect of Xingnaojing injection in treating patients with acute cer-ebral infarction and its influence on serum matrix metalloproteinase 9 (MMP -9),nitric oxide (NO)and nitric oxide synthase (NOS).Methods 112 patients with acute cerebral infarction were randomly divided into two groups by ran-dom number table method,56 cases in each group.The control group received routine treatment for acute cerebral infarction.The observation group received routine treatment plus intravenous injection Xingnaojing.The changes of NIHSS score,motor function score and sensory function score in the two groups were compared before and after treat-ment.The serum levels of MMP -9,NOS and NO in the two groups were observed before and after treatment.Results The total effective rate of observation group was 82.14%,which was significantly higher than 64.29% in the control group (χ2 =4.553,P <0.05).After treatment,NIHSS scores of the observation group were significantly lower than the control group (t =5.082,5.522,6.253,all P <0.01 ).After treatment for 3 weeks,the motor function score (69.54 ±17.82)points,sensory function score (30.58 ±7.94)points of the observation group were significantly higher than (52.93 ±14.66)points and (23.08 ±6.31)points in the control group (t =5.387,5.534,all P <0.01).After treatment,the serum MMP -9,NOS,NO levels of the observation group were significantly lower than the control group (all P <0.01 ).Conclusion Xingnaojing injection in the treatment of acute cerebral infarction has good curative effect,it can promote the recovery of neurological function,improve motor function and sensory function, and by adjusting the MMP -9,NOS,NO levels to protect the nerve and blood vessel function.

Objective:To study the relationship among serum uric acid (SUA)level,brachial-ankle pulse wave veloci-ty (baPWV)and severity of coronary disease.Methods:A total of 121 inpatients,who hospitalized in our depart-ment of cardiology from Jan 2011 to Jul 2012,were enrolled.According to results of coronary angiography,they were divided into coronary heart disease (CHD)group (n=59)and normal control group (n=62),CHD group was further divided into single vessel coronary disease group (n=30,single vessel group),double-vessel coronary disease group (n=14,double-vessel group)and multi-vessel coronary disease group (n=15,multi-vessel group).SUA level and baPWV were compared among all groups,and their relationship with degree of coronary disease was analyzed. Results:SUA concentration gradually rose along with number of diseased coronary vessels increased,it's (349.26± 96.58)μmol/L,(400.37±70.96)μmol/L,(517.57±85.26)μmol/L and (602.60±77.03)μmol/L in normal con-trol,single vessel,double-vessel and multi-vessel group respectively,P<0.05 or <0.01;baPWV gradually rose a-long with number of diseased coronary vessels increased,compared with normal control group,there was significant increase in baPWV [(1499.04±193.82)cm/s vs.(1885.32±319.73)cm/s,(2036.00±406.40)cm/s,(2171.03± 348.53)cm/s]in single vessel,double-vessel and multi-vessel group,that of double-vessel group was significantly higher than that of single vessel group,P<0.05~<0.01.Logistic regression analysis indicated that SUA and baP-WV were independent risk factors for CHD morbidity (OR=13.011,14.008,P=0.000).Conclusion:Serum uric acid level and pulse wave velocity possess important clinical application value for predicting CHD and its severity.

Objective To establish a quality control for Tianmatoufengling Capsule. Methods HPLC method was taken for the determination of Gastrodine in Tianmatoufengling Capsule,A Kromasil C18 column was used,with methanol 0.05% Calcium phosphace(2.5∶97.5) as the mobile phase,and its flow rate was 1.0 mL/min. The detection wavelength was 220 nm. TLC method was used for the identification of Tianmatoufengling Capsule. Results Under the used chromatographic condition,Tianmatoufengling had fine linear responses(r =0.999 9) in the concentration range of 6.016～96.256 ?g/mL,the average recovery was 100.02%,RSD was 2.24%. The method used in TLC-qualitative analysis was simple and sensitive with high specificity,four ingredients identified in Tianmatoufengling Capsule could be detected and all showed specific spots. Conclusion The method employed in this study was convenient and reliable with good repetition. It can be used for quality control in production of Tianmatoufengling Capsule.

Objective The purpose of this study is to investigate the surgical procedures and curative effect through a small incision on both sides of the scapula for surgical resection the ribs tumor which located un -der ventral surface of the scapula .Methods The group included 11 patients,6 males and 5 females,aged 18 to 49 years.Lesions were bilateral scapula deep square 2~4 single rib bone tumors .Among them there were five ca-ses of fibrous dysplasia ,case of bone cyst ,2 cases of plasma cell tumors and metastatic tumors in 3 cases.Surgery incision through cross-scapular ,to determine which lesions ribs ,cutting the lesions on both sides of the stripping and junction.Results The group obtained a satisfactory excision extension ,one case appeared hemopneumotho-rax postoperatively.Secondly,none of patients occured local recurrence .Thirdly,two patients with metastatic carci-noma died after one year because of multiple metastases .Conclusion Straddling scapular notch is the ideal way to cut off the rib tumor of ventral surface of the scapula .Possessing advantages of a little damage and effect on the function,and rapid recovery .

To explore the change in gene expressions of p38 mitogen-activated protein kinase (p38MAPK) and its upstream signal-regulated molecule (mkk3 and mkk6) in normal skin versus hypertrophic scars underlying its potentially biological significance. Total RNAs were isolated from 8 specimens of hypertrophic scars and 8 specimens of normal skin, then they were purified to mRNAs, and the gene expression of mkk3, mkk6 and p38MAPK was examined with reverse transcription-polymerase chain reaction (RT-PCR). The results showed that the intensities of gene expression of mkk6 and p38MAPK were weak in normal skins, while the expression of these 2 genes was significantly elevated in hypertrophic scars compared with normal skins (P0.05). In hypertrophic scar, the elevation of mkk6 and p38MAPK gene expression, which plays pilot roles in cell proliferation, may be one of the mechanisms controlling the formation of hypertrophic scars.

Objective To study the chemical constituents in the seeds of Zizyphus jujuba var.spinosa.Methods The compounds were isolated by silica gel column chromatography and their structures were established by spectroscopic methods.Results A new keto-dammarane type saponin,jujuboside H(Ⅰ),along with three known compounds protojujuboside A(Ⅱ),spinosin(Ⅲ),and betulic acid(Ⅳ)were isolated.Conclusion Compound Ⅰ is a new compound named jujuboside H.

Objective To detect the methylation status of DLEC1 promoter in the tissue and serum of colorectal cancer(CRC) patients and to evaluate its clinical relevance.Methods Genomic DNA was extracted from the tissues(cancer tissue and corresponding adjacent normal tissue) and sera of 71 CRC patients;serum genomic DNA was also obtained from 20 patients with benign gastrointestinal diseases and 20 healthy donors.Promoter methylation status of DLEC1 gene was detected by methylation-specific polymerase chain reaction(MSP).Results The incidence of aberrant methylation of DLEC1 promoter was 45.1%(32/71) in CRC tissues,which was significantly higher than that in the adjacent normal tissues(7.1%,4/56)(P

Objective To explore how different sex affect the clinical feature and apneic event,sleep time in patient with sleep apnea hypopnea syndrome.Methods According to the analysis of polysomnography(PSG),133 male and 47 women with chief complain of snoring were divided into four groups simple snor,mild SAS,moderate SAS and severe SAS group.Compared related clinical feature and PSG data among them ,we found the difference in it.Results Male patient have more complication than female patient (P

To investigate the different expression of Fas, Fas L and Caspase 3 in hypertrophic scars and normal skins in order to explore their influences on scar formation, the expression intensity and distribution of Fas, Fas L and Caspase 3 were detected by immunohistochemistry and pathological methods in 8 cases of hypertrophic scars and 8 cases of normal skins. In normal skins, Fas and Fas L were mainly located in the cellular membranes and cytoplasms of epidermal cells and some fibroblasts. Caspase 3 was mostly distributed in epidermal basal cells and some fibroblasts. In hypertrophic scars, Fas and Fas L were principally existed in the membranes and cytoplasms of epidermal keratinocytes. The positive cellular ratio of two proteins was significantly reduced ( P

The purpose of this study was to investigate the localization and expression characteristics of phosphorylated form of extracellular-signal regulated-protein kinasel/2 (p-ERKl/2), Ras and C-fos in skin at different development stages and to explore their potential biological significance. Immunohistochemistry and pathological methods were used to detect the expression intensity and distribution of p-ERKl/2, Ras and C-fos in skin of 8 fetuses with different gestational ages (13 to 31 weeks) and 8 adults. Positive immunohistochemical signals of p-ERK1/2, Ras and C-fos. could be found in fetal and postnatal skins. Along with the increment in gestational age, the positive cell rates of p-ERK1/2, Ras and C-fos in the skin elevated progressively. In skins derived from the fetus of late-trimester pregnancy and adult, the positive rates of these three proteins were significantly increased in comparison with skin from the early-trimester fetus (P