Objective To investigate the elimination effect of propofol on the reactive oxygen species(ROS) in patients with acute craniocerebral injury (ACI). Methods Forty patients with ACI were randomly divided into propofol group receiving propofol anesthesia and ?-OH group as control . Electron spin resonance(ESR) spectroscopy was used to determine the plasma contents of oxygen free radical (OFR), and the plasma contents of lipid peroxides(LPO) and nitrogen oxide (NO) were determined with chemical method. Blood samples were collected before anesthesia and 2, 4 hours after starting operation. Results The plasma contents of NO, OFR and LPO significantly increased before operation in patients with ACI compared with healthy subjects(all P

Objective To compare the curative effect of several surgical treatments for closed urethral injury.Methods A retrospective study was done on 200 cases of closed urethral injury treated with various methods from June 2002 to June 2012,including 44 cases of anterior urethral injury treated with endoscopic transurethral realignment (anterior endoscopic group),32 cases of anterior urethral injury with transperineal urethral repair (repair group),42 cases of posterior urethral injury with transperineal urethral termino-terminal anastomosis (anastomosis group),35 cases of posterior urethral injury with endoscopic transurethral realignment (posterior endoscopic group),and 47 cases of posterior urethral injury with endoscopic transurethral and transvesical realignment (combined endoscopic group).Amount of bleeding,operation time,hospital stay as well as expense,and rate of urethral stricture and erectile dysfunction (ED) in treatment of anterior and posterior urethral injury were compared respectively.Curativeeffect and merits and faults of each treatment were observed.Results Anterior endoscopic group achieved less bleeding [(10 ± 5) ml∶ (100 ± 20) ml,P ＜ 0.05],shorter operation time and hospital stay [(15 ± 6) min∶ (40 ± 15) min,(14.2 ± 1.8) min∶ (28.5 ± 1.5) min respectively,P ＜ 0.05],lower hospital expense and urethral stricture rate[(4 715.42 ± 225.43) RMB∶ (6 886.46 ± 246.78) RMB,39％ ∶ 63％ respectively,P ＜ 0.05] as compared with repair group,but there was no statistical difference in ED rate between the two groups.Whereas in anastomosis group,the most bleeding [(200 ± 30) ml],longest operation time [(60 ± 20) minutes] and hospital stay [(28.7 ± 2.3) days],and highest hospital expense [(6 964.27 ± 275.69) RMB] and urethral stricture rate (95 ％) were detected (P ＜ 0.05).Posterior endoscopic group presented less bleeding [(15 ± 6) ml∶ (50 ± 12) ml,P ＜ 0.05] and shorter operation time [(21.4 ± 1.7) min∶ (21.2 ± 1.5) min,P ＜ 0.05] as compared with combined endoscopic group,but there were no statistical differences in hospital stay as well as expense and urethral stricture rate between the two groups.Moreover,ED rate was not statistically different among anastomosis group,posterior endoscopic group and combined endoscopic group.Conclusions Endoscopic surgery is superior to traditional surgery in terms of blood loss,operation time,hospital stay,and hospital cost.Besides,urethral stricture and ED rates are closely related to the location and severity of injury.

In medical equipment purchasing process, massive non-price factors' influences conduce to quite quantities of risks in the medical equipment purchase. To control the risks,this paper proposes the project management, the supplier management, the equipment traceability management and related suggestions. Management on purchaser is also discussed, which mainly includes professional stress analysis and the countermeasures research.

Aim To determine whether there existed melatonin receptor (MR) in adult hepatoma tissue and to observe its property of binding.Methods Immuno-histochemistry was used to detect MR and identify its subcellular distribution. Specific binding and kinetic analyses of melatonin receptor were measured by radioligand binding assay.Results The results of immunohistochemistry showed that MT1 and MT2 existed in membrane, cytosol and nucleus of the hepatoma cells, but the expression of them principally localized in the membrane and cytosol. The specific binding assay properties of MR were presented as follows: the maximum binding capacity (B_(max)) was (0.29±0.07) pmol·g~(-1) protein. Equilibrium dissociation constant (K_d) was (48.7±6.5) pmol·L~(-1). The trait of MR, saturation and reversibility, was detected by ~(125)I-Mel specific binding kinetic analyses.Conclusions MR exists in adult hepatoma cells, furthermore, the subtypes of MR (MT1 and MT2) coexist in the membrane and cytosol, respectively,whose characters of the specific binding sites were low binding capacity,high affinity,saturation and reversibility.

Objective To investigate the possibility of transplantation of adrenocortical cells to treat insufficiency of adrenocortical function using poly-?-hybroxybutyric acid (PHB) as cell carrier.Methods Adrenocortical cells were seeded to PHB and then implanted into rats bearing bilateral adrenalectomy, while adenocortical cells implanted into the similar rats as a contrast. The changes of blood corticosterone and aldostenone and local histological changes in these rats were observed.Results The blood corticosterone and aldostenone were elevated after transplantation. The levels of blood corticosterone and aldostenone in PHB group were higher than that of control group. Histological study showed that adenocortical cells in PHB grew well.Conclusion The implanted adrenocortical cells within PHB can not only survive in rat body but also secrete corticostenone and aldostenrone which can satisfy the need of adrenalectomized rats. It is more effective to use PHB in adrenocortical cell transplantation than that cell implanted singly.

Objective To evaluate the clinical application of transendoscopic Doppler ultrasonogra-phy in various gastrointestinal diseases. Methods Transendoscopic Doppler ultrasonography was performed in 59 patients with various gastrointestinal diseases. Results The Doppler signals were detected beneath the peptic ulcer base in 29 of 39 patients (74. 4% ) . For acute ulcer bleeding and non - bleeding, the Doppler - positive rates were 87. 5% and 65. 2% , respectively, and the measured depth of vessels were 0. 49 mm and 0. 65mm (P

Objective To study the relationship between cerebral vascular disease (CVD) and meteorological factors. Methods From 1998 to 2002, 6186 patients with CVD in Qingdao urban were studied. There were 1179 cases of intracerebral hemorrhage (CH), 4821cases of cerebral infarction (CI) and 186 cases of subarachnoid hemorrage (SAH). The relationship between the onset of CVD and meteorological factors including temperature, air pressure, relative humidity, wind velocity was analyzed by circular distribution analysis, Pearson correlation and multiple stepwise regression. Results High incidence of CH was found in winter and the mean angle was in January ((P

Objective To investigate the effect of PHB on the proliferation and secretion function of adrenocortical cells and the efficiency of PHB as a cell carrier in transplantation of adrneocortical cells. Methods Adrenocortical cells from rat adrenal gland were separated and cultured in vitro. The effect of PHB on the proliferation and secrete function of adrenocortical cells were evaluated by MTT and RIA method. Adrenocortical cells were seeded into PHB. After cells were cultured in vitro for about 7 days, they were implanted into rats subject to bilateral adrenalectomy. The changes of blood corticosterone and aldosterone and local histological changes were observed. Results Adrenocortical cells could grow and passage on PHB. PHB had no effect on the proliferation and secretion function of adrenocortical cells. Adrenocortical cells from autograft, allograft and xenograft greatly suppressed the proliferation of spleen cells (P

Objective To compare the effect and safety between cemented and uncemented long-stem hip arthroplasty for treatment of unstable intertrochanteric fracture in the elderly.Methods A retrospective review was made on 70 cases (70-87 years old) undergone artificial long-stem hip arthroplasty of unstable intertrochanteric fracture between June 2012 and June 2014.According to the material of prostheses,the cases were assigned to cemented group (n =36) and uncemented group (n =34).Operation-related index,postoperative complications,ambulation time,Harris hip score and prosthesis stability and excellent results were compared between the two groups.Results There were no significant differences in operation time,bleeding volume and ambulation time between the two groups (P ＞ 0.05).Postoperative drainage volume and blood transfusion volume in cemented group [(277.4 ± 22.0) ml and (245.1 ± 17.8) ml] were lower than these in uncemented group[(294.2 ± 33.7) ml and (262.8 ± 30.9)ml] (P ＜0.05).Harris scores in cemented group were higher than these in uncemented group 1 and 3 months after operation(P ＜0.05),and there were no significant differences 6 and 12 months after operation(P ＞ 0.05).Complication rate had no significant difference between the two groups (P ＞ 0.05).Two cases were died of cement poisoning,one case of subsequent acute myocardial infarction,and two cases of subsequent acute cerebral infarction.Sixty-five cases were followed up for 12-36 months,which showed no complications such as prosthesis loosening,displacement or infection.Prosthesis stability and treatment excellent rate between the two groups were not significantly different (P ＞ 0.05).Conclusions Clinical efficacy of the two treatments is comparable,including good function recovery and early off-bed activity.Cemented long-stem hip arthroplasty reduces blood requirements,but the risk of cement poisoning exists.

AIM: To assess the impact of weaver gene on neuronal development,protein expression and vitality. METHODS: DNA encoding the wild-type and mutant ion channel was introduced into immortalized tyrosine hydroxylase-positive CNS-derived neurons named CAD(Cath.a-differentiated,a variant of Cath.a. Cath.a was established by targeted oncogenesis in transgenic mice) cells. DNA clone, immunostaining and Western blotting were used. Three different concentrations (0 25 mg/L, 0 5 mg/L, or 1 0 mg/L) of Girk2 and wv Girk2 expression plasmids were transfected into the CAD cells. The number of transfected cycling cells, protein synthesis and neurites growth were observed between two groups. RESULTS: The number of transfected cycling CAD cells with high concentration of wv Girk2 reduced to about 60%, compared to Girk2-transfected cells. Low concentration of wv Girk2 did not cause cell death but reduced the protein production of transfected genes. Neurite growth was also affected by wv Girk2. MK-801 and Kir2.3 altered the effect of wv Girk2. CONCLUSION: The results indicate that wv Girk2 functions as a blocker in weaver animals, which blockes the wv Girk2 channel to rescue the cells from death. Our data also suggest that the presence of channels and the level of wv Girk2 may have a significant impact on the fate of cells containing wv Girk2.