Objective To explore the pH conditions for human immunodeficiency virus(HIV) detection(ELISA) in the finished products of human immunoglobulin(pH 4) for intravenous injection(IVIG), in order to control the residual HIV risk of IVIG and ensure the safety of blood products.Methods The HIV antibody reference materials and antigen reference materials were diluted to the detection limit level using different pH buffer solutions. The detection of HIV reference materials at different pH and detection limit levels by the reagent kit were observed to determine the appropriate pH detection range of the reagent kit. The pH range of the finished product was adjusted to the appropriate range of the reagent kit by using different concentrations of Tris-HCl solutions, and then the HIV antibody reference materials and HIV antigen reference materials were diluted to the detection limit level to obtain the appropriate pH detection range of the product.Results The A450/630 values of the HIV antibody reference materials and HIV antigen reference materials with detection buffer at pH 7. 0-9. 0 were similar to those of the normal saline(NS) group, and the suitable pH range for the HIV kit used was pH 7. 0-9. 0. IVIG 4. 0 was diluted with0. 1 mol/L Tris-HCl solution to the appropriate pH range of the reagent kit as the diluent. The A450/630 value of antibody 0. 25 NCU/mL in IVIG pH 7. 0-8. 0 group was higher than the cutoff value and significantly higher than that in the IVIG pH 4. 0 group.The amount of 0. 1 mol/L Tris-HCl solution added accounted for more than 1/5 of the IVIG volume. IVIG 4. 0 was diluted with 1 mol/L Tris-HCl solution to the appropriate pH range of the reagent kit as the diluent. The A450/630value of antibody 0. 25 NCU/mL and antigen 1. 5 U/mL levels was higher than the cutoff value in the IVIG pH 7. 0-9. 0 group. The amount of 1 mol/L Tris-HCl solution added was only 1/28-1/37 of the total detection volume, and had little effect on the effective detection content of the test sample.Conclusion Compared to the detection conditions of pH 4. 0, pH 7. 5-8. 0 is more suitable for HIV detection in IVIG. 1 mol/L Tris-HCl solution was used to adjust the pH of IVIG to 7. 5-8. 0 for HIV detection(ELISA)in IVIG, which effectively solves the problem that the pH bias in IVIG finished product is not conducive to HIV detection, with no effect on the detection volume of the finished product, thus significantly improving the accuracy and reliability of the detection results.

OBJECTIVE:To establish a fingerprint analysis method of Pinellia ternata from the west of Hubei province.METHODS:The samples of Pinellia ternata from different areas harvested at different time and processed by different methods were analyzed by GC method to establish their fingerprint data.RESULTS: The fingerprints of Pinellia ternata have been established using GC data of the chromatographic peaks of five phytosterols from various samples,and these samples had a similarity of between 0.877 and 0.999.CONCLUSION: The fingerprints of phytosterols of Pinellia ternata are stable thus can be used as a new method for the quality control of Pinellia ternata.

Objective: To develop a scale to measure attitudes toward homosexuals in Chinese university students.Methods:Based on surveys and literatures, we conducted the pre-test and test for accessing the construct validity of the scale by EFA ( exploratory factor analysis) and CFA (confirmatory factor analysis) in 1010 Chinese university students.Results: There were three components that could explain 63.84% of the total variance with loading between 0.501 and 0.851 in the scale.The three components could explain 46.67%,10.27% and 6.90% of the variance separately.They were named cognitive tolerance, emotional tolerance and behavior tolerance.The scale had NFI 0.979,NNFI 0.981,CFI 0.985,RMSEA 0.073 by CFA.The Cronbach'?coefficient was 0.931 and the test-retest reliability was 0.839 (with P

Objective:To investigate the impact of self-concealment,self-disclosure,coping style and per- ceived social support on university students'loneliness.Methods:Loneliness and related factors were assessed among 482 university students using scales including UCLA Loneliness Scale,Self-concealment Scale(SCS),Self-disclosure Index(SDI),Simplified Coping Style Questionnaire(SCSQ)and Perceived Social Support Scale(PSSS).Results: The level of university students'loneliness was not high(36.5?7.4);males experienced more loneliness than fe- males(37.4?7.5/35.4?7.3,F=8.25,P0.05). Regression analysis showed that SCS,SCSQ and PSSS predicted UCLA effectively(?=0.207,-0.218,0.157, -0.380).The testing of mediating effect indicated that SCS had direct and indirect impact on UCLA through nega- tive coping style and PSSS;SDI had only indirect impact on UCLA through positive coping style and PSSS.Conclusion:SCS,SDI,SCSQ and PSSS are important factors influencing UCLA,and the intervention of univer- sity students'loneliness should focus on these variables.

Curdlan is a water insoluble exopolysaccharide produced by Alcaligenes faecalis under nitrogen-limiting conditions. After excretion, the polysaccharide is attached the cell wall. Thus enhancement of biomass production during the cell growth phase is important to curdlan production. A strategy of increasing nitrogen source to improve biomass production was adopted for curdlan production by Alcaligenes faecalis (ATCC 31749). In the batch fermentation of curdlan, a relatively higher NH4Cl level of 3.6 g/L with continuous glucose feeding increased the cell density leading to improvement of curdlan production. However, excessive NH4Cl would inhibit curdlan production and biomass production was not improved significantly. In addition, feeding of ammonia water at the initial phase replaced NaOH solution to control pH at 7.0. Subsequently, feeding of NaOH solution was resumed to control pH at 5.6 for curdlan production after ammonia was consumed. As a result, biomass production and curdlan yield were both enhanced remarkably. Feeding of ammonia water during the first 24 h led to biomass production of 18.8 g/L. However, higher cell density did not lead to increase in curdlan production. The maximum curdlan production (72 g/L) was obtained by feeding ammonia water for the first 14 h, during which the cell density was about 11.9 g/L.
Alcaligenes faecalis ; cytology ; metabolism ; Ammonium Chloride ; pharmacology ; Cell Culture Techniques ; methods ; Cell Proliferation ; Fermentation ; beta-Glucans ; metabolism

[Objective] To establish a real-time fluorescence quantitative PCR nucleic acid detection method of human parvovirus B19 and validate the method systematically. [Methods] Specific primers and probes for the highly conserved regions of the three genotypes of B19 virus were designed, and B19 quantitative amplification standard curves were established. The accuracy, precision (repeatability and intermediate precision), linear range, quantification limit, detection limit, specificity, anti cross contamination, genotyping and anti-interference ability of this method were verified. [Results] When the quantitative reference range for B19 virus was 2.0×10¹ to 1.0×10⁸ IU/mL, a double logarithmic regression analysis was performed between the measured values and the theoretical values, and the regression equation R²≥0.98 showed good linear correlation. The quantification limit was 20 IU/mL, with a detection rate of 100%. The detection limit was 10 IU/mL, and the detection rate is 95.23%. Three genotypes of B19 virus samples can be effectively detected. The plasma of seven non B19 pathogens, including hepatitis A virus, hepatitis B virus, hepatitis C virus, human immuno-deficiency virus, human cytomegalovirus, hepatitis E virus and Treponema pallidum, was non reactive and has good species specificity. Simultaneously, in the presence of seven other concurrent pathogens, positive samples with a weak positive concentration of E3 IU/mL could be stably detected, and the B19 nucleic acid testing method was not interfered with. When the hemoglobin concentration was 431 mg/dL, triglycerides (1 269 turbidity) and unconjugated bilirubin concentration was 20 mg/dL, this method was non reactive for all three common plasma interfering substances. In the presence of three common plasma interfering substances, positive samples with a weak positive concentration of E3 IU/mL could be stably detected, and the B19 nucleic acid testing method was not interfered with. The deviation between the detection values of standard substances at two concentration levels of S1 (E5 IU/mL) and S2 (E4 IU/mL) and the target values were≤±0.5 log value. The CV values of positive sample 1 (concentration level E5 IU/mL) and positive sample 2 (concentration level E4 IU/mL) for daily precision confirmation and continuous 5-day intra-day precision confirmation were both≤5%. [Conclusion] This method has strong specificity, high sensitivity, wide linear range, stability, reliability and high accuracy, and can be used for the detection of human parvovirus B19 nucleic acid in plasma.