1.Blood-pricking combined with moxibustion on positive reaction point for 35 cases of greater occipital neuralgia.
Chinese Acupuncture & Moxibustion 2015;35(3):252-252
Acupuncture Points
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Adult
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Bloodletting
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Combined Modality Therapy
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Female
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Headache
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therapy
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Humans
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Male
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Middle Aged
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Moxibustion
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Neuralgia
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therapy
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Young Adult
4.Inhibitory effects of recombinant human Mullerian inhibiting substance on cell proliferation in two human ovarian carcinoma cell lines
Lan XIAO ; Yuebo YANG ; Tian LI ; Xiaoyun WANG ; Xiaomao LI
Chinese Journal of Pathophysiology 2010;26(1):127-131
AIM:To investigate the inhibitory effects of recombinant human Mullerian inhibiting substance on cell proliferation in human ovarian carcinoma cells (OVCAR8 and SKOV3 cell lines). METHODS:The expression of MISIIR protein and the localization of MISIIR protein were analyzed by Western blotting and confocal spectral microscopy,respectively. Cell apoptosis and cell cycle were detected by flow cytometry (FCM). Cell viability was determined via MTT method. Clone formation test was used to detect oncogenicity in vitro.RESULTS:The MISIIR protein expression in OVCAR8 cells but not in SKOV3 cells was observed. MISIIR expression was seen on the OVCAR8 cell surface and in the cytoplasm with both antibodies. After treated with rhMIS for 48 h,the cell viability was significantly decreased in OVCAR8 cells. rhMIS inhibited the oncogenicity of OVCAR8 cells greatly. The cell apoptosis of OVCAR8 cell exposed to 10 mg/L rhMIS was (31.3±2.1)%,and OVCAR8 cells in the G_1 phase were increased by (70.4±3.0)%. Compared to SKOV3 cells the differences were significant (P<0.01). CONCLUSION:Recombinant human Mullerian inhibiting substance suppresses the growth of MISIIR-positive ovarian cancer cells by inducing apoptosis and cell cycle arrest. We predict that rhMIS might be a new target to treat human ovarian malignancies.
6.EXPRESSION OF AROMATASE CYCOCHROME P - 450 IN THE NEURAL STEM CELLS AND THE EARLY DIFFERENTIATED CELLS
Lan XIAO ; Wei LI ; Wenqin CAI ; Xiangdong ZHOU
Acta Anatomica Sinica 1989;0(S1):-
Objective To explore the gene expression in the neural stem cells as well as the cells after the clone was differentiated. Methods Neural stem cell culture, Immunocytochemistry and RT-PCR technique were used. Results There was Aromatase expression in the neural stem cells, after the stem cells were differentiated, the Aromatase strongly expressed in the neruons and weakly expressed in the astrocytes. The aromatase gene in these cells was expressed by means of the brain-specific promoter 1. 4.Conclusion It may provid a new clue for the source of estrogen in the central nerve system.
7.A Comparative Study of Pictures and Words in Subliminal Affective Priming Effect
Lan LI ; Liushuang HUANG ; Lihui XIAO ; Ping ZHOU
Chinese Journal of Clinical Psychology 2006;0(05):-
Objective:To explore whether there is a significant difference between the affective priming effect of pictures and words.Methods:The study used pictures taken from the international picture system and Chinese words as the priming-stimuli, and took college students as subjects, then primed positive, negative and neutral emotions, and asked subjects to accomplish stroop task and self-rating emotion task.The study took the design between 2(types of material) ?3(types of emotion).Results:①In the stroop task, there were significant differences between the reaction time and error rate of different emotions(F=36.216,P=0.000
8.Epstein-Barr virus positive anaplastic-like plasmacytoma: report of a case.
Wen-sheng LI ; Xiao-ge ZHOU ; Jian-lan XIE
Chinese Journal of Pathology 2013;42(3):203-204
ADP-ribosyl Cyclase 1
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metabolism
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Aged
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Epstein-Barr Virus Infections
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Herpesvirus 4, Human
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isolation & purification
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Humans
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Interferon Regulatory Factors
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metabolism
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Ki-67 Antigen
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metabolism
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Male
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Nasal Cavity
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Nose Neoplasms
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metabolism
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pathology
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surgery
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virology
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Plasmacytoma
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metabolism
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pathology
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surgery
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virology
9.Pyothorax-associated lymphoma: report of a case.
Li-hua WU ; Jian-lan XIE ; Xiao-ge ZHOU
Chinese Journal of Pathology 2009;38(3):205-206
Adult
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Antigens, CD20
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metabolism
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Empyema, Pleural
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complications
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diagnostic imaging
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pathology
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virology
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Epstein-Barr Virus Infections
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Humans
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Ki-67 Antigen
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metabolism
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Lymphoma, Large B-Cell, Diffuse
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metabolism
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pathology
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surgery
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virology
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Male
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Pleural Neoplasms
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complications
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metabolism
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pathology
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surgery
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virology
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Radiography
10.Cyr61 expression influences cancer cell proliferation and apoptosis via PI3K pathway in human ovarian carcinoma cells
Lan XIAO ; Chan HE ; Xin LI ; Ying CHEN ; Jiade ZHOU
Chinese Journal of Obstetrics and Gynecology 2012;47(8):616-620
Objective To investigate the relationship between cysteine-rich protein 61 ( Cyr61 ) and phosphatidylinositol 3-kinase ( PI3K ) signal pathway on cell proliferation and apoptotic in human ovarian carcinoma cells.Methods Recombinant human Cyr61 (rhCyr61) was pretreated with ovarian carcinoma cells.The expression of Cyr61 protein was detected by confocal spectral microscopy.Then treated the ovarian carcinoma cells with PI3K transduction inhibitors (LY294002) for 24 hours.Cell apoptosis was detected by flow cytometry (FCM).Cell viability was determined by methyl thiazolyl tetrazolium (MTT) method.The mRNA expressions of Cyr61,the protein levels of protein kinase B ( PKB),phospho-PKB and Cyr61 were assaved by real time-PCR and western blot analysis,respectively.Results The Cyr61 and phospho-PKB protein expression in two ovarian carcinoma cells (OV2008 and OVCAR-3 ) were increased in rhCyr61pretreated group.The decreasing of cell apoptosis [ ( 1.4 ±0.9)%,(2.1 ± 1.0)% ] and increasing of cell proliferation [ ( 124.0 ± 1.8)%,( 133.0 ±2.2)% ] was detected in the same time,compared with negative control group,there were significant difference ( P < 0.05 ).After exposed to LY294002 for 24 hours,the apoptosis rate of OV2008 and OVCAR-3 in pretreated with rhCyr61 group exposed to LY294002 were (21.1 ± 1.6)% and (26.4 ± 1.5 )%,respectively.Cells viability [ (59.0 ± 2.3 )%,(51.0 ± 2.0)% ]was also significantly decreased in OV2008 and OVCAR-3 pretreated with rhCyr61 cells.Meanwhile,the mRNA expressions of Cyr61 (3.2 ± 0.8,6.2 ± 1.1 ) and the protein levels of phospho-PKB and Cyr61 were greatly decreased.Compared with negative control group,there were significant difference in OV2008 and OVCAR-3 cells (all P < 0.0l ).Conclusions The activation of PI3K intracellular signaling pathways may lead to up-regulation of Cyr61 expression.Block PI3K signal pathway could significantly inhibit the expression of Cyr61,and may promote the apoptotic effects and inhibit the cell growth of ovarian carcinoma cells.