Aged ; Humans ; Kallikreins ; analysis ; Male ; Middle Aged ; Prognosis ; Prostate-Specific Antigen ; analysis ; Prostatic Neoplasms ; diagnosis

Objective To provide anatomical evidence for a safe osteotomy line and direction in periacetabular osteotomy.Methods The pelvic CT images of 18 adults were obtained.With the help of Mimics software,three-dimensional CT reconstruction was performed,and periacetabular osteotomy was simulated.The length of osteotomy lines on the ilium,ischium and iliac columna posterior was measured,and the relationship between osteotomy lines and anatomic landmarks was explored. Results The length of osteotomy lines on the ilium,ischium and iliac columna posterior was(50.96?7.09) mm,(36.46?5.92)mm and(55.28?6.42) mm,respectively.The angles between the osteotomy line on the ilium and that on the iliac columna posterior,between the osteotomy line on the ischium and that on the iliac columna posterior,and between the osteotomy line on the iliac columna posterior and quadrilateral surface were 120.21??5.96?,115.49??6.46? and 60.31??4.96?,respectively.And the real periacetabular osteotomy has been performed on the pelvic specimens for verification.The vertical distances between the ending point of osteotomy line on the ilium and iliopectineal line,between osteotomy line on the ischium and iliopectineal line,and between the osteotomy line on the iliac columna posterior and greater sciatic notch were(11.98?5.26) mm,(45.08?8.59) mm and(18.52?6.49) mm,respectively. Conclusion The length and angles of osteotomy lines in periacetabular osteotomy can be well measured by three-dimensional reconstruction,and the relationship between the osteotomy lines and anatomic landmarks can also be well displayed,which provides anatomical data for the clinical application of periacetabular osteotomy.

Polymerase chain reaction (PCR) is one of the common techniques in molecular biology, which can amplify nucleic acids through the cycle of denaturation, annealing and extension. Based on the principle of common PCR, rapid PCR is to realize the amplification of nucleic acids in less time without affecting the specificity, sensitivity and fidelity of the reaction. A lot of research work in this field has been going on in recent years. This article will make a review of the development of rapid PCR with emphases on the improvement of DNA polymerase, the choice of additives and the improvement of thermocyclers.

BACKGROUND:The tourniquet is usualy fuly used or bilateraly used in partial time during bilateral total knee arthroplasty. However, very few people try to use it on one side and on the other side in partial time.
OBJECTIVE:To investigate the effective strategy of using a tourniquet in simultaneous bilateral total knee arthroplasty.
METHODS:80 patients (160 knees) with severe osteoarthritis who underwent simultaneous bilateral total knee arthroplasty in the Department of Orthopedics, Chinese PLA Medical School from January to December 2013 were divided into two groups according to the different tourniquet strategies. In test group (n=40), left knees did not receive tourniquet, and right knees received tourniquet in partial time. In control group (n=40), al knees received tourniquet in the whole time. The perioperative and postoperative blood loss, 3-day postoperative thigh sweling rate and pain visual analog scale, 3-week and 1-year postoperative Knee Society Score were recorded in both groups.
RESULTS AND CONCLUSION:There were no statisticaly significant differences between two groups in perioperative total blood loss (P > 0.05). However, postoperative blood loss in test group was less than that in control group (P < 0.05). The visual analog scale scores were significantly lower in the test group than in the control group at 3 days after surgery (P < 0.05). Visual analog scale scores on the left side were lower than on the right side in the test group at 3 days post surgery (P < 0.05). Bilateral thigh sweling rate was significantly lower in the test group than in the control group at 3 days after surgery (P < 0.05). The thigh sweling rate was lower on the left side than on the right side in the test group at 3 days after surgery (P < 0.05). Knee Society Score was higher in the test group than in the control group in the early stage, and no significant difference in long-term Knee Society Score was detected (P > 0.05). These findings verify that taking the strategy that the first knee without tourniquet and the second knee with part time tourniquet technique in simultaneous bilateral total knee arthroplasty wil aleviate pain and sweling after operation and promote early functional rehabilitation without increasing the perioperative total blood loss.

Objective To explore the changes and relationship of serum insulin-like growth factor-1(IGF-1) and leptin in children with growth hormone deficiency(GHD).Method Serum IGF-1 and leptin levels were measured by immunoradiometric assay in 20 prepuberal normal children and 23 children with GHD.Results Serum IGF-1 level in GHD group(51.158?29.988)?g/L was significantly lower than that in controls(112.680?41.540)?g/L.Serum leptin levels in GHD group(6.002?2.204)?g/L was significantly higher than that in controls(4.523?2.204)?g/L.There was no significant correlation between serum IGF-1 and leptin.Conclusion IGF-1 and leptin regulate growth in children with GHD in their individual way.

Objective To investigate the relationship between the different embolization agent and the spinal cord ischemic injury. Methods Bilateral of intercostal arteries were embolized with gelatin sponge and lipiodol to block the blood supply of spinal cord. To detect the somatosensory evoked potentials(SEPs), 6 canines in both groups of gelatin sponge and lipiodol were treated by bilateral intercostal arteries embolization. The median nerve and fibular nerve were recorded and the ratio of lower extremity to upper extremity SEPs amplitude (LE/UE ratio) were calculated; the results were compared with the pathologic findings and the motor function. Results The baseline, LE/UE, evoked potential amplitude ratios in all dogs before operation ranged from 0.22 to 0.92 with a combined total mean of 0.49?0.07. The SEPs changes at 30 minutes post operation were more obvious than pre operation in gelatin sponge group and lipiodol group with statistic significance. The LE/UE ratio of gelatin sponge group recovered one hour after operation, but this was absent in lipiodol group. So did the latency SEPs changes. Conclusion The SEPs could not be used to evaluate the motor function of spinal cord comprehensively.

OBJECTIVE: By summary and analysis of rigid internal fixation for the treatment of maxillofacial fractures incision and exposure, investigate the plate reasonable surgical approach of fracture reduction and fixation titanium. METHOD: Summary of the 76 surgical cases, Counting the statistics of the number that the surgery ways choose by facial incision and fractures location, analysis of the indications for surgery and the advantages and disadvantages of various surgical approaches. Followed up for more than six months, in order to observe the recovery of occlusal function and the facial cosmetic results. RESULT: The upper jaw or cheek bone has the more possibility in facial fracture, which used of a small incision hidden under the lip gingival sulcus and lower eyelid. After six months, the facial wound healing recover in good occlusal with no obvious scarring. CONCLUSION Reasonable choice of surgical incision can make the fracture site exposure and the facial aesthetic effect into account.
Adult ; Bone Plates ; Facial Bones ; injuries ; Female ; Fracture Fixation, Internal ; instrumentation ; methods ; Fractures, Bone ; surgery ; Humans ; Male ; Retrospective Studies ; Titanium

Objective: To describe the clinical consequences of acute infusion reactions associated with cetuximab treatment and analyze corresponding managing experience to explore the prevention and treatment approaches. Methods: Clinic staff identified 24 patients with advanced gastric cancer and colorectal cancer who had received chemotherapy combined with cetuximab treatment and experienced an acute infusion reaction. Their clinical manifestation and infusion reaction management procedures were retrospectively analyzed. Results: Acute infusion reactions at grade I - II were observed in 4 of 24 cases (16.7%), occurring during the first 5-10 min after the first administration of cetuximab. Patients complained chills, chest tightness, and low fever (37-38.5°C) after 0.5 h infusion. The symptoms were self relieved after 1-2 h. When cetuximab was recharged at reduced infusion rate and with close monitoring, no acute infusion reactions were observed in the 4 patients. Conclusion: Discontinuation of cetuximab therapy is not necessary for the patients who experienced grade I - II infusion reaction mild-to-moderate reactions can be managed by reduction of the infusion rate and close observation of the symptoms. Patients who experienced grade III or IV infusion reactions may require permanent discontinuation of cetuximab therapy.

Objective To study the anti-fibrotic function and mechanism of peroxisome proliferator activated receptorγ(PPARγ) in connective tissue disease-interstitial lung disease (CTD-ILD).Methods The expression of PPARγin lungs was analyzed in 37 cases with CTD-ILD and 20 control cases by immunohistochemistry.Changes in α-SMA levels were analyzed by Western blotting,and acetylation of Smad3 and Smad3 or PPARγ combined with P300 were analyzed by IP-WB.The data was analyzed by one-way ANOVA,t test or Mann-Whitney test.Results PPARγ' expression in the lung of CTD-ILD was lower than the controls [0.92％(1.44％),3.50％(1.94)％,respectively; Z=-8.924,P＜0.01].Different concentration of PPARγ (0,1,5,10,20,40 pmol/L) ligandinhibited the marked elevation of the protein α-SMA induced by TGF-β1 in a concentration-dependent manner (0.918 ±0.062,0.852±0.042,0.725 ±0.057,0.678 ±0.042,0.418 ±0.022,0.456±0.029; P＜0.05 or P＜0.01).However,this response was blocked by a selective antagonist PPARγ signaling GW9662 (0.946±0.087 vs 0.538±0.120,P＜0.01).Acetylation of Smad3 expression was increased when TGF-β1 was putted into lung fibroblasts after 60,90 and 180 min (0.565±0.047,1.127±0.101,0.873±0.022,0.614±0.407; all P＜0.05).The combination of Smad3 with P300 was also increased (1.46±0.12,0.98±0.09; P＜0.05),compared with the controls.But the ligand of PPARγ could block this effect (0.62±0.10,1.46±0.12; P＜0.05).Meanwhile,the combination of PPARγ and P300 was increased (0.94±0.05,0.76±0.22; P＜0.05).Conclusion PPARγ may play a physiologic role in the regulation of anti-fibrosis response.Its function may be realized by its competition with Smad3 combined with P300.

Objective To analyze the outcomes and prognostic factors associated with the death of systemic lupus erythematosus (SLE) patients admitted to the intensive care unit (ICU). Methods During June 1996 to June 2007, all SLE patients admitted to the ICU were included. Patients were excluded if the diagnosis of SLE was established at or after ICU admission. A multivariate logistic regression model was applied using variables that were associated with death in the univariate analysis. Results A total of 101 patients meeting the criteria were included. The mortality rate was 48.6%. The most common causes of admission was lung disorder with acute respiratory distress syndrome (ARDS). Multivariate logistic regression analysis suggested that SLICC/ACR DI>7.7 (OR=6.87), APACHE Ⅲ≥21 (OR=29.8), lung disorders with ARDS (OR =55.81 ), septic shock (OR =32.22 ), intracranial haemorrhage (OR =57.35 ), hypocytopenia (OR = 5.89), mean equivalent prednisone dose>25 mg/d (OR=7.65) and prolonged tracheal intubation (OR=5.98) were signi-ficantly associated with death. Whereas sex, age, SLEDAI >27, gastrointestinal bleeding, the cumulative dosage of CTX higher than 1.0 g, pulse intravenous methylprednisolone therapy were not associated with death. Conclusion The mortality rate of critically ill SLE patients in ICU is very high. SLICC/ACR DI> 7.7, APACHE Ⅲ≥21, lung disorders with ARDS, septic shock, intracraniai haemorrhage, average prednisone equivalent dosage higher than 25mg/d and prolonged tracheal intubation (longer than 4 days) are negative prognostic factors in SLE patients admitted to the ICU.