5. Optimal rotation time and pitch study of CT angiography for arterial occlusive diseases of lower limbs
Academic Journal of Second Military Medical University 2010;31(2):165-168
Objective: To identify the optimal rotation time and pitch of 64-slice spiral CT angiography for arterial occlusive diseases (AOD) of the lower limbs. Methods: We performed test-bolus in eighty patients with AOD of the lower limbs (48 with intermittent claudication and 32 with ischemic rest pain or ulceration) to confirm the aortic peak time, popliteal artery peak time and aortopopliteal bolus transit time. The patients were randomly assigned to receive two sets of scan protocols for lower limb arterial 64-slice spiral CT angiography (CTA) examination (n = 40), protocol A, with a gantry rotation time of 330 ms and a pitch of 1.0; protocol B, with a gantry rotation time of 500 ms and a pitch of 0. 85. Two experienced radiologists independently evaluated the image quality of the lower limb artery. Results: Great differences in the time to peak enhancement in the aorta (14-33 s) and popliteal arteries (20-48 s) and the aortopopliteal bolus transit time (4-24 s) were found between different AOD patients. Wide overlap of the time to peak enhancement and transit time was observed between intermittent claudication patients and ischemic rest pain or ulceration patients. The qualities of segment images were rated as excellent 63.3% (152/240), good 30.0% (72/240), and poor 30.0% (72/240) for protocol A, and excellent 98. 3% (234/238) and good 1. 7% (4/238) for protocol B, with no poor images in protocol B. Conclusion: 64-slice CTA with a gantry rotation time of 500 ms, a pitch of 0. 85, scan time>30 s, and with the contrast agent injection time maintained at 35 s, can obtain better image of lower limb arteries in patients with AOD of the lower limbs.
9.Percutaneous kyphoplasty for vertebral compression fractures in patients with osteoporosis
Dechang XIAO ; Weilin LI ; Ming PENG
Orthopedic Journal of China 2006;0(18):-
[Objective]To evaluate the efficacy of percutaneous kyphoplasty (PKP) in managing vertebral compression fractures due to osteoporosis(OVCF).[Method]Thirty-one OVCFs patients (46 vertebrae) undergoing PKP were retrospectively analyzed.In these patients,there were 9 males and 22 females who were from 61 to 78 years old with averaged 68 years,and the injured vertebrae were from T7 to L3.All the patients had plain X-rays and CT scan as well as MR images preoperatively.With all of the patients,local infiltration anesthesia were performed,20 were injected bilaterally while 11 unilaterally.Before and after operarion,the visual analogue scale (VAS) score was estimated and sagittal index(SI) as well as Cobb′s angle of the vertebrae were measured.The data were statistically analyzed with the method of paired t-test.[Result]The duration of follow up after operetion were 6 to 30 months with a mean of 18 months.The VAS score reduced from 8.7 before operation to 2.5 after operation (P
10.Expression of HLA-G5 in healthy people and the recipients of renal and liver transplantation
Li XIAO ; Bingyi SHI ; Ming CAI
Medical Journal of Chinese People's Liberation Army 1982;0(01):-
Objective To investigate the expression of human leukocyte antigen-G5 (HLA-G5) in healthy Chinese people and the recipients of renal and liver transplantation. The regulating mechanism of the expression of HLA-G5 was discussed by comparing the expression of HLA-G5 in the healthy people with that in renal and liver transplantation recipients. Furthermore, the changing regularity with time was studied by kinesis supervising the expression of HLA-G5 in renal and liver transplantation recipients. Methods The peripheral blood samples (3ml) from 30 health people, 50 recipients of liver transplantation (liver function was stable 3 months after liver transplantation) and 50 recipients of renal transplantation (renal function was stable 3 months after renal transplantation) were collected. Peripheral blood samples were also collected in same amount from 33 recipients of renal and liver transplantation before operation and 1, 4 and 12 weeks and 1 year after operation. The HLA-G5 of all serum samples was analyzed by ELISA. Results For 30 healthy people, the OD value of HLA-G5 in 28 people was below 0.5, for which the contents were defined as 0.0ng/ml according to standard and the contents for the other 2 people were 8ng/ml and 9ng/ml, respectively. 16 of 50 recipients undergone liver transplantation were positive for the expression of HLA-G5, the positive ratio was 32%. The contents in 4 recipients were higher than 30ng/ml. 10 of 50 recipients of renal transplantation were positive in the expression of HLA-G5, the positive ratio was 20%. The contents in one recipient were higher than 25ng/ml. The average contents in sera of healthy people, recipients of liver or renal transplantation were 0.56?0.20ng/ml, 8.34?1.50ng/ml and 3.26?0.25ng/ml, respectively. For 33 recipients of liver or renal transplantation, the expression of HLA-G5 was detected by ELISA, and it was found that one recipient the expression of HLA-G5 was positive before operation and within 1 week after operation; expression of HLA-G5 was positive in 4 recipients within 4 weeks after operation; expression of HLA-G5 was positive within 12 weeks after operation in 12 recipients; and the expression of HLA-G5 was positive within 1 year after operation in 11 recipients. Conclusion The expression of HLA-G5 in healthy people is low. There are correlation between the expression of HLA-G5 and immunotolerance to transplants. In minor rejection condition after transplantation, there are different expression levels of HLA-G5, and it is higher after liver transplantation than!renal transplantation. The time for expression of HLA-G5 corresponds with the time for mRNA of HLA-G5 transcription into protein, and it is about 15-60 days, with 60 days as the peak time.