1.Progress of treating hepatic failure with induced pluripotent stem cell-derived hepatocytes
Lei XIAO ; Jungen LI ; Yunlai LIU
International Journal of Biomedical Engineering 2014;37(1):49-52
Hepatocyte transplantation is effective in the treatment of liver failure caused by a variety of factors.In pace with the progress of the study of induced pluripotent stem cells and its differentiation technology,a new method has arisen to obtain a great number of safe hepatocytes with biological function,which are suitable for seed cells of hepatocyte transplantation.In this article,we review the latest research progress about induced pluripotent stem cell-derived hepatocytes being transplanted to treat liver failure.
2.Correlation of visfatin and L-PGDS with lower extremity arteriosclerosis
Lei XIAO ; Junhai LI ; Xiujun ZHANG
Chinese Journal of Biochemical Pharmaceutics 2017;37(1):298-301
Objective To investigate the relationship between lipocalintype prostaglandin dsynthase(L-PGDS) and visfatin in the lower extremity atherosclerotic plaque. Methods Collected from February 2014 to February 2016 in vascular surgery of our hospital 40 cases of femoral artery atherosclerotic plaque intima specimens (observation group), 20 cases of splenic artery, superior mesenteric artery samples (control group), the expression of visfatin and L-PGDS protein were detected by immunohistochemical staining, and the expression of visfatin and L-PGDS mRNA were determined by RT-PCR. Results The observation group visfatin protein expression was (121.42±11.07), significantly higher than the control group (72.07±12.81), and L-PGDS protein expression was (87.93±9.73), significantly lower than the control group (107.04±10.58), the difference was statistically significant (P<0.05). The relative expression of visfatin mRNA in the observation group was (0.321±0.024), which was significant higher than that of the control group (0.217±0.031), while L-PGDS mRNA was (0.203±0.018), significantly lower than the control group (0.314±0.029), the difference was statistically significant (P<0.05); The expression of L-PGDS protein was negatively correlated with the expression of visfatin protein in the plaque tissue (r=-0.617, P<0.05), visfatin mRNA and L-PGDS mRNA were negatively correlated (r=-0.645, P< 0.05). Conclusion Visfatin and L-PGDS in lower extremity atherosclerosis occurrence and development have an important relationship, both of them may play an antagonistic role.
3.Role of Cripto-1 protein in development and target therapy of breast cancer.
Ting LEI ; Xiao-jing GUO ; Li FU
Chinese Journal of Pathology 2010;39(1):66-68
Animals
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Antibodies, Monoclonal
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metabolism
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therapeutic use
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Biomarkers, Tumor
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metabolism
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Breast Neoplasms
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metabolism
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pathology
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therapy
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Drug Delivery Systems
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Epidermal Growth Factor
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immunology
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metabolism
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physiology
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Epithelial-Mesenchymal Transition
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GPI-Linked Proteins
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Humans
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Immunotherapy
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methods
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Intercellular Signaling Peptides and Proteins
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Membrane Glycoproteins
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immunology
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metabolism
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physiology
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Neoplasm Invasiveness
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Neoplasm Metastasis
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Neoplasm Proteins
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immunology
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metabolism
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physiology
4.One case of acute arsenic poisoning by absorption through skin wound.
Xin LI ; Xiongbin XIAO ; Jinggui XU ; Li LI ; Lei XIE
Chinese Journal of Industrial Hygiene and Occupational Diseases 2014;32(2):138-138
Adult
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Arsenic Poisoning
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etiology
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Arsenicals
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Humans
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Male
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Skin
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injuries
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Skin Absorption
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Wounds and Injuries
5.HPLC enantioseparation, absolute configuration determination and anti-HIV-1 activity of (±)-F18 enantiomers.
Lei-lei ZHANG ; Hai XUE ; Li LI ; Xiao-fan LU ; Zhi-wei CHEN ; Gang LU
Acta Pharmaceutica Sinica 2015;50(6):733-737
Racemic (±)-F18 (10-chloromethyl-11-demethyl-12-oxo-calanolide A), an analog of nature product (+)-calanolide A, is a new anti-HIV-1 nonnucleoside reverse transcript inhibitor (NNRTI). A successful enantioseparation of (±)-F18 offering (R)-F18 and (S)-F18 was achieved by a chiral stationary phase prepared HPLC. Their absolute configurations were determined by measurement of their electronic circular dichroisms combined with modem quantum-chemical calculations. Further investigation revealed that (R)-F18 and (S)-F18 shared a similar anti-HIV activities, however, (R)-F18 was more potent than (S)-F18 against wild-type virus, K101E mutation and P225H mutation pseudoviruses.
Anti-HIV Agents
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chemistry
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Chromatography, High Pressure Liquid
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HIV-1
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drug effects
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Pyranocoumarins
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chemistry
6.Expression and significance of aryl hydrocarbon receptor in atrial tissues of patients with atrial fibrillation
Jianming LEI ; Hua XIAO ; Qiang LI ; Xiao WEI ; Jingwen GUO ; Minghan XIAO
Chinese Journal of Pathophysiology 2017;33(5):826-831
AIM:To investigate the expression of aryl hydrocarbon receptor (AhR) in atrial tissues of the patients with rheumatic heart disease (RHD), and the effects of AhR on rheumatic atrial fibrosis.METHODS:Right atrial specimens obtained from the patients with RHD requiring valve replacement surgery were divided into chronic atrial fibrillation (RHD+cAF, n=11) group and sinus rhythm (RHD+sinus rhythm, n=25) group.The patients with congenital heart disease (CHD) and sinus rhythm (CHD+sinus rhythm, n=12) who underwent heart surgery served as controls.The collagen volume fraction in the atrial specimens was examined by Masson`s trichrome staining.The protein expression and distribution of AhR, AhR nuclear translocator (ARNT) and CYP1A1 were detected by the methods of immunohistochemistry and Western blot.The mRNA expression of AhR, ARNT and CYP1A1 was detected by real-time fluorescence quantitative PCR.RESULTS:Compared with CHD+sinus rhythm group, the collagen content and the expression of AhR, ARNT and CYP1A1 were significantly increased in RHD+sinus rhythm group and RHD+cAF group.Compared with RHD+sinus rhythm group, the collagen content and the expression of AhR, ARNT and CYP1A1 were significantly increased in RHD+cAF group (P<0.05).CONCLUSION:The expression of AhR is correlated with the degree of fibrosis.The expression of AhR/ARNT/CYP1A1 is increased in atrial tissues of patients with RHD, suggesting that AhR/ARNT/CYP1A1 should be involved in atrial fibrosis of the patient with RHD.
7.Feasibility assessment of visual quality analyzer KR - 1 W guiding personalized aspheric lOL implantation
Xiao-Li, WANG ; Qian, LI ; Xiao-Lei, TANG ; Ying-Chun, LÜ ; Li, GUO
International Eye Science 2015;(1):43-45
Abstract?AlM:To discuss the feasibility of using the visual quality analyzer KR - 1W to guide the relatively personalized aspheric intraocular lens ( lOL ) implants to make the whole eye spherical aberration close to 0. 1μm.?METHODS: ln this prospective case series study, the corneal spherical aberration with 6mm aperture of 73 patients (100 eyes) was measured with KR-1W Visual Function Analyzer 1d before surgery. For the sake of the whole postoperative spherical aberration were close to 0. 1μm, 9 cases ( 16 eyes ) with corneal spherical aberration <0. 15μm were implanted Sofport Advanced Optic lOL, named AO group;45 cases ( 57 eyes ) with corneal spherical aberration 0. 25~0. 3μm were implanted AcrySof lQ lOL, named lQ group;19 cases (27 eyes) with corneal spherical aberration > 0. 35μm were implanted Tecnis ZA9003 lOL, named Tecnis group. Aspherical lOL was implanted after phacoemulsification through a cornea 2. 75mm incision without suture. Uncorrected visual acuity, beat corrected visual acuity, spherical aberration of the whole eye and jnternal optics (mainly lOL) at 6mm pupil diameter were examined at 3mo postoperatively. The relevant data were analyzed using t-test and variance analysis.?RESULTS: The whole ocular spherical aberration at 6mm pupil diameter in all postoperative were 0. 084 ± 0. 032μm;in Tecnis group, the data were 0. 091 ± 0. 021μm;in AO group, the data were 0. 0814-0. 013μm;lQ group were0. 093 ± 0. 042μm. There was no significantly different between the predicted value and actual value of ocular spherical aberration at 6 mm pupil diameter in all postoperative ( t = 1. 932, P = 0. 061 ) and in the three groups. The difference value in the predicted values of the preoperative spherical aberrations of the whole eye and the actual values after surgery was 0. 013±0. 041μm; there was no statistically significant difference ( F=2. 537, P=0. 091 ) . Respectively compared the uncorrected visual acuity and besta corrected visual acuity among three groups of postoperative, no significant difference were found (F=0. 897, P=0. 421;F=1. 423, P=0. 097).?CONCLUSlON: Personality selection of aspheric lOL based on preoperative corneal spherical aberration of patients is feasible and produces satisfactory target postoperative total spherical aberration.
8.Determination of Peoniflorin in Shenshaogankang Oral Liquid by HPLC
Hongliang LI ; Jinhu WU ; Yuqing XIAO ; Long LEI
China Pharmacy 2005;0(17):-
OBJECTIVE:To establish HPLC method for the determination of the content of paeoniflorin in Shenshao?gankang oral liquid.METHODS:Paeoniflorin was separated on Kromasil C 18 ;methanol-0.05mol/L KH 2 PO 4 solution-avantin(60∶173∶4,which was adjusted to pH=4.0with acetic acid)was taken as the mobile phase with a flow rate at1.0ml/min and detection wavelength at230nm.RESULTS:Good linear relationship was achieved when the detection concentration range of peoniflorin was0.027~0.230mg/ml(r=0.9999);The average recovery of peoniflorin was100.97%(RSD=1.28%,n=5). CONCLUSION:The method was simple,rapid,precise and reproducible,which can be used as the quality control of Shenshaogankang oral liquid.
9.Innovating capability training-based construction of information literacy education course system for military postgraduates
Xiaoru YANG ; Jin CHENG ; Fangwei LI ; Lei WANG ; Xiao WANG
Chinese Journal of Medical Library and Information Science 2017;26(3):60-64
After the problems in military postgraduate information literacy education were analyzed, the construction of a systematic and coherent information literacy education course system for military postgraduates in close combination with their subjects was proposed by setting up multidimensional education target, designing three-stage foundation-subject-innovation course, combining traditional literature retrieval course and embedded professional course, combining case teaching, heuristic discussion and innovative talent information literacy training approach, by infiltrating the physical and virtual space both in and outside university, and by participating in the study and research activities of postgraduates.
10.Stress changes in inferior facet joint after artificial lumbar disc replacement
Kanghua LI ; Wenfeng XIAO ; Jianzhong HU ; Guanghua LEI
Chinese Journal of Tissue Engineering Research 2007;0(04):-
0.05).CONCLUSION:①There are not significantly changes of the stress in the inferior facet joint after ADR.②ADR will not add negative influence to the inferior facet joint when it rebuilds the biomechanical characteristics of lumbar spine.