Adenoma, Oxyphilic ; diagnosis ; metabolism ; Carcinoma, Renal Cell ; diagnosis ; metabolism ; Humans ; Immunohistochemistry ; methods ; Keratins ; analysis ; Kidney Neoplasms ; diagnosis ; metabolism ; Proto-Oncogene Proteins c-kit ; analysis ; Sensitivity and Specificity ; WT1 Proteins ; analysis ; Wilms Tumor ; diagnosis ; metabolism

Carcinoma, Renal Cell ; classification ; diagnosis ; genetics ; pathology ; Chromosomes, Human ; Humans ; Immunohistochemistry ; methods ; Kidney Neoplasms ; classification ; diagnosis ; genetics ; pathology ; Translocation, Genetic

Basic Helix-Loop-Helix Leucine Zipper Transcription Factors ; genetics ; metabolism ; Carcinoma, Renal Cell ; genetics ; metabolism ; Cell Cycle Proteins ; genetics ; metabolism ; Humans ; Melanoma ; genetics ; metabolism ; Microphthalmia-Associated Transcription Factor ; genetics ; metabolism ; Neoplasm Proteins ; genetics ; metabolism ; Oncogene Proteins, Fusion ; genetics ; metabolism ; Perivascular Epithelioid Cell Neoplasms ; genetics ; metabolism ; Sarcoma, Clear Cell ; genetics ; metabolism ; Translocation, Genetic

Adenocarcinoma, Mucinous ; metabolism ; pathology ; Calbindin 2 ; Carcinoma, Endometrioid ; metabolism ; pathology ; Diagnosis, Differential ; Female ; Humans ; Immunohistochemistry ; methods ; Inhibins ; metabolism ; Keratin-7 ; metabolism ; Mucin-1 ; metabolism ; Ovarian Neoplasms ; metabolism ; pathology ; S100 Calcium Binding Protein G ; metabolism ; Sex Cord-Gonadal Stromal Tumors ; metabolism ; pathology

Hepatitis, Viral, Human ; pathology ; Humans ; Liver ; pathology ; Liver Cirrhosis ; pathology ; Liver Neoplasms ; genetics ; pathology ; Loss of Heterozygosity ; Microsatellite Instability ; Polymorphism, Single Nucleotide

Antigens, Neoplasm ; metabolism ; Carbonic Anhydrase IX ; Carbonic Anhydrases ; metabolism ; Carcinoma, Renal Cell ; diagnosis ; metabolism ; pathology ; Humans ; Kidney Neoplasms ; diagnosis ; metabolism ; pathology ; Matrix Metalloproteinase 2 ; metabolism ; Matrix Metalloproteinase 9 ; metabolism ; Neoplasm Grading ; Prognosis ; RNA-Binding Proteins ; metabolism ; Tumor Burden ; Vascular Endothelial Growth Factor A ; metabolism ; Von Hippel-Lindau Tumor Suppressor Protein ; metabolism

In order to improve diagnostic method to Babesia gibsoni infection,gene recombination technique was used in our study.By this technique,we constructed and expressed recombinant apical membrane antigen-1(BgAMA-1),and recombinant BgAMA-1 was used in ELISA experiment as diagnostic antigen.The results showed the recombinant expressed plasmid was triumphantly constructed and the recombinant antigen BgAMA-1 had a high specificity in ELISA test.These results demonstrate that the recombinant BgAMA-1 might be a useful diagnostic reagent for detection of antibodies to B.gibsoni in dogs.

Objective To study the experience of laparoscopic repair in gastric and duodenum perforation. Methods 35 patients with gastric and duodenum perforation were performed laparoscopic repair.Results 34 pati- ets with gastric and duodenum perforation were safely operated.1 case with perforation of gastric carcinoma was con- verted to open for radial gastrectomy.The mean time of hospitalization was 7.5 days.There was no intraoperative and postoperative complications.Pathological examination showed 4 patients with perforation of gastric ulcer and one with perforation of gastric carcinoma.Conclusion Laparoseopic repair was one of the safe,quick recovery and little suffering treatment for duodenum perforation.

Objective To evaluate the effect of live frog skin in the treatment of deep partial thickness and full thickness burn. Methods 159 burned patients were randomly divided into two groups: wounds treated with frog skin or suckling pig skin. Wounds were covered by frog skin(35 cases) or by suckling pig skin(31 cases) after surgical debridement or when the crust began to dissolve for patients with deep partial thickness burns, and by frog skin (49 cases) or by sucking pig skin(44 cases) after surgical debridment or autologous skin grafting and escharetomy for patients with full thickness burns. There was no significant difference between the two groups in mean age, burn area and burn type. Results Compared with the suckling pig skin, frog skin could remarkably decrease days of wound recovery and antibiotics administration, and reduced incidence of wound infections and rejection in patients with full thickness burns. Conclusions Frog skin possessed more powerful anti-infection ability, and rare rejection phenomenon in the treatment of burn wounds. It may be a suitable biological dressing for burn wounds.

Objective To observe changes of spacial learning and memory and the ultrastructure of hippocampus in perimenopausal depression model rats. Methods Thirty-six female SD rats were randomly divided into four groups(9 in each). Normal group wasn't given any stress, and stressed group was given isolation-housing in combination with chronic unexpected mild stress (CUMS) for 21 days. Ovariectomized group was given ovary resecting, and model group was made by resecting the ovaries of female rats, then giving isolation-housing in combination with 21 days of CUMS. Morris water maze test were used to test rats' behaviors. Light microscope and transmission electron microscope were used to observe the tissular structure of hippocampus. Results After ovaries resecting and 21 days of CUMS,the escape periods of rats in model group during the first three days ( (44.08 ± 18.29)s; ( 38.80 ± 19.07 ) s; ( 22.74 ± 14.13 ) s) were longer than ovariectomizd group ( (43.68 ± 10. 37 ) s; ( 20.28 ±17.75)s;(21.22 ± 11.91 )s] and normal group( (34.50 ± 11.08)s;(21.42 ± 14.32) s;( 13.94 ±9.76) s). HE staining showed that hippocampal pyramidal cell layer became thinner in rat model of depression,the gap between cells increased,and the cells arrayed in disorder,or loosely,even a large number of cells were lost. Transmission electron microscope showed that in rats of model group, hippocampal neurons were less, nuclear collapsed, the whole nuclear membrane was fuzzy and there were some local fractures in the nuclear membrane. Mitochondria within the cytoplasm were swelling and their ridges disappeared. Conclusion The decline of spacial learning and memory abilities in perimenopausal depression model rats might result from pathological changes in hippocampus.