Objective: To investigate the relationship between the autophagy and the sensitivity to DDP (cisplatin) in epithelial ovarian cancer SKOV3 and SKOV3/DDP cells. Methods: The formation of autophagosomes in SKOV3 and SKOV3/DDP cells was observed under a TEM (transmission electron microscope). MTT assay was used to screen the dose of nontoxic reaction of autophagy inhibitor 3-MA (3-methyladenine) and to examine the sensitivity to DDP in SKOV3 and SKOV3/DDP cells after treatment with the combination of DDP and 3-MA. The apoptotic rate and the expressions of autophagic marker protein LC3 (microtubule-associated protein 1 light chain 3) and autophagy-related protein Beclin1 in SKOV3 and SKOV3/DDP cells after treatment with DDP and 3-MA alone and the combination of DDP and 3-MA were detected by flow cytometry and Western blotting, respectively. Results: Much more autophagosomes in SKOV3/DDP cells were observed as compared with that in SKOV3 cells. As compared with DDP treatment alone, when DDP was used in combination with 3-MA, the IC50 (half inhibitory concentration) value of DDP for SKOV3/DDP cells was decreased from (17.71±0.15) μg/mL to (8.85±0.11) μg/mL (P < 0.05), and the apoptotic rate was increased from (6.93±0.94) % to (14.82±0.85) % (P < 0.05), as well as the expression levels of LC3 and Beclin1 proteins in SKOV3/DDP cells were significantly decreased (P < 0.05), but there were no significant changes could be observed in SKOV3 cells. Conclusion: The increased activity of autophagy is related to DDP-resistance of ovarian cancer cells. The inhibition of autophagy may enhance the sensitivity to DDP in SKOV3/DDP cells. Copyright © 2013 by TUMOR.

Objective To understand the features of lymphatic vessel,and to summarize the foundation and mechanism of the promotion and inhibition of tumor lymphangiogenesis recorded on the current studies of animal ex- periments and clinical researches.Methods The related literatures of the structural features of lymphatic vessel, lymphatic endothelial molecular markers,the origin of lymphatic tumors,the molecular mechanisms and regulatory factors were reviewed,and the relationship between tumor lymphangiogenesis and lymphatic metastasis,the treat- ment targeting at the formation of the anti-tumor lymphatic vessel and its existing problems were also analyzed.Re- suits Hyperplasia of lymphatic vessels occurred during the process of tumor formation and progression.The struc- tural features of the lymphatic vessels in the tumor were conducive to tumor lymphatic metastasis.In recent years, methods of anti-lymphangiogenesis and inhibition of tumor lymphatic metastasis had achieved considerable success in animal experiments.However,there were still a lot of problems need to be solved.Conclusion Tumor lymphangio- genesis has a significantly positive correlation between tumor lymphatic metastasis and patients'prognosis,which may indicate that treatment against the formation of tumor lymphatic vessel maybe effective.

The notational DSA was first proposed for the application as a managing tool of neuronradiology in the early 70's of 20th centery.Later on,three dimensional image reconstruction(3D DSA) was aquired along with the progressing development of technique and equipment devices.As a rather practical instrument,it has been utilized successfully in the diagnosis and management of head and neck vascular disorders,and abdominal parenchymal diseases during recent years.We comprehensively intoduce the application of rotational DSA for the diagnosis and management in hepatic diseases.

Objective To compare the ocular parameters measured by 2 biometry devices Tomey OA-2000 and IOLMaster in cataract eyes.Methods A total of 74 cataract patients (74 eyes) were included in this prospective study.Ocular parameters,including axial length (AL),keratometry (K),anterior chamber depth (ACD) and white to white (WTW),were obtained from Tomey OA-2000 and IOLMaster,respectively.Paired t-test was applied to analyze the differences of ocular parameters from Tomey OA-2000 and IOLMaster,while Bland-Altman plot was used to assess the agreement of these ocular parameters.Results The AL,flat K,steep K,mean K,central corneal thickness (CCT),ACD,lens thickness,pupil diameter and WTW values measured by Tomey OA-2000 were (24.26 ± 2.71) mm,(44.09 ± 1.76) D,(45.01 ± 1.73) D,(44.55 ± 1.72)D,(518.41 ±34.18) μm,(3.07 ±0.48)mm,(4.48 ±0.61)mm,(4.07 ±0.95)mm and (11.50 ± 0.52)mm respectively.Values of AL,flat K,steep K,mean K,ACD and WTW from IOLMaster were (24.33 ± 2.69) mm,(44.21 ± 1.74) mm,(45.15 ± 1.76) D,(44.69 ± 1.74) D,(3.04 ± 0.46)mm and (11.65 ± 0.41) mm respectively.The difference values of AL,flat K,steep K,mean K,ACD and WTW between Tomey OA-2000 and IOLMaster were (-0.07 ±0.10) mm (t =-5.99,P＜0.01),(-0.12 ± 0.21) D (t =-4.14,P＜0.01),(-0.14 ± 0.20)D (t =-5.88,P＜0.01),(-0.12 ±0.13) D (t =-7.44,P＜0.01),(0.03 ±0.14) mm (t=1.99,P=0.05) and (-0.14±0.32) mm (t=-3.73,P＜0.01) respectively.The 95％ limits of agreement (LoA) of the AL,flat K,steep K,mean K,ACD obtained from the two devices was 0.27 mm,0.50 D,0.53 D,0.38 D,0.30 mm,respectively,and the agreement was good;95％ LoA of WTW was 0.78 mm,and the consistency was poor.Conclusion The differences of AL,K values and ACD between Tomey OA-2000 and IOLMaster were small,suggesting the agreement is unified.

Objective To observe the changes of extracellular matrix(ECM) production and hypertrophy of glomerular mesangial cells(GMCs) induced by high glucose(HG),and the inhibitory role of myriocin(ISP-1).Methods GMC cultured with normal glucose(5.6 mmol/L D-Glucose,NG),HG(25.2 mmol/L D-Glucose) and HG plus ISP-1(100 mg/L) for different durations(0,24,48,(72 h)).The sizes of GMC were indicated by forward scatter intensity,measured by flow cytometery,and the levels of fibronection(FN),collagen Ⅳ(Col Ⅳ),laminin(LN),precollagen Ⅲ(Pcol Ⅲ) and hyaluronic acid(HA) in the supernatant of cultured GMC were detec-(ted) by ELISA.Results Compared with NG,HG could induce GMC hypertrophy(P

Adult ; Antigens, CD20 ; metabolism ; Empyema, Pleural ; complications ; diagnostic imaging ; pathology ; virology ; Epstein-Barr Virus Infections ; Humans ; Ki-67 Antigen ; metabolism ; Lymphoma, Large B-Cell, Diffuse ; metabolism ; pathology ; surgery ; virology ; Male ; Pleural Neoplasms ; complications ; metabolism ; pathology ; surgery ; virology ; Radiography

Objective To study the nutritional evaluation and clinical effects of perioperative enteral nutrition (EN) versus parenteral nutrition support (PN) in elderly patients undergoing gastrectomy for gastric carcinoma.The safety,feasibility and superiority were also compared between EN and PN.Methods Totally 50 cases (aged 65 years and over) undergoing gastrectomy for gastric carcinoma in Department of Gastrointestinal Surgery of Beijing Hospital were recruited and divided randomly into two groups of EN and PN (25 cases for each).NRS2002 nutritional assessment scoring system was used to evaluate the patients.The patients in EN group were given Rui Su at 2 d before surgery,then Wei wo,Bai pu li and Rui su were sequentially given from 12 h after the surgery.The patients in PN group were received total parenteral nutrition (TPN) via central venous for 7 days.Results The average absolute lymphocytes[(1.22±0.23) ×109/L and (1.31±0.27) ×109/L vs.(1.02±0.21) × 109/ L and (1.14 ± 0.23 × 10)9/L],pre-albumin[(151.442± 48.15) mg/ L and (167.38± 46.23) mg/ L vs.(115.22 ± 47.34) mg/L and (131.27 ± 43.58) mg/L] and transferring [(1.71±0.33)g /L and (1.83±0.31)g/L vs.(1.50±0.32)g/L and (1.65±0.32)g/L,all P＜0.05] levels after surgery of 3 d and 7 d in EN group were higher than those of PN group (P＜0.05).The CRP levels at 3 d and 7 d after surgery were increased in PN group compared with EN group [(63.71±35.26)mg/L and (41.58 ± 22.55)mg/L vs.(54.26±29.13)mg/L and (18.12 ±14.35)mg/L,P＜0.05].The blood sugar and insulin levels of EN group at 3 d and 7 d after surgery were (5.93±1.12) mmol/L and (5.61± 1.03)mmol/L,(6.72±5.14) U/L and (6.21±2.63) U/L,which were decreased compared to PN group (8.31 ± 2.62) mmol/L and (8.85 ± 2.92) mmol/L,(12.81±7.26) U/L and (15.21±8.24) U/L (P＜0.05).ALT in the PN group was (38.43±18.37) U/L at 7 d after surgery,higher than EN group (23.91± 14.82) U/L(P＜0.05).The perioperative average costs of EN group was (2714.5± 1391.7) yuan,lower than PN group (5041.6± 3007.7) yuan (P＜0.05).For the patients complicated with delayed gastric emptying after surgery,nutrition cost reduction was more evident.There were no significant difference between the two groups of patients in the liver and kidney function changes and postoperative complications.Conclusions It is safe and feasible for elderly gastric cancer patients with perioperative EN sequential therapy in view of its reduced stress response of surgical trauma and insulin resistance,improvement of hyperglycemia,reduction of medical costs compared with PN for elderly gastric cancer patients.

Aged ; Carcinoma, Squamous Cell ; surgery ; Female ; Forearm ; surgery ; Free Tissue Flaps ; Humans ; Laryngeal Neoplasms ; surgery ; Laryngectomy ; Male ; Middle Aged ; Reconstructive Surgical Procedures ; methods ; Skin Transplantation ; methods

Animals ; Asthma ; drug therapy ; metabolism ; Genistein ; pharmacology ; therapeutic use ; Guinea Pigs ; Interleukin-4 ; genetics ; metabolism ; Janus Kinase 1 ; genetics ; metabolism ; Lung ; metabolism ; Male ; Protein Kinase Inhibitors ; pharmacology ; therapeutic use