ObjectiveTo investigate the effects of moxibustion on the expression of tryptophan hydroxylase 2(TPH2) mRNA in median raphe nuclei and the activity of monoamine oxidase (MAO) in serum and cortex in depression model rats and to explore its mechanism.MethodsFemale SD rats were randomly divided into control group(n=6),model group(n=7) and moxibustion group(n=7).The rat depression model was induced by giving isolation-housing in combination with chronic unexpected mild stress(CUMS) for 21 days.The hemi-quantitative reverse transcription-polymerase chain reaction(RT-PCR) was employed to detect the relative expression of TPH2 mRNA,colorimetry was used to test the activity of MAO.ResultsThe expression of TPH2 mRNA in median raphe nuclei in rats of the moxibustion group was higher than that in the model group( relative dentisty value:(0.3759 ± 0.0272)vs(0.2573 ± 0.0581 ),P ＜ 0.05 ).The activity of MAO in serum and cortex was reduced in rats of the moxibustion group( ( 1.919 ± 1.243 ) U/ml,( 1292.048 ± 90.072 ) U/mg protein,respectively) compared with that in the model group ( (4.117 ± 2.727 ) U/ml,( 1441.345 ± 117.050 ) U/mg protein,respectively).ConclusionMoxibustion has the effects of improving depression,increasing the expression of TPH2 mRNA in median raphe nuclei and reducing the activity of MAO in serum and cortex might be one of its mechanisms.

Objective To study and summarizes the methods ofthe standardized hospital infection manage -ment technology in order to prevent and control hospital infection scientifically .Methods Taking the chance of im-provingthe execution of the standardized hospital infection management issued by the Ministry of Health ,we realized organizational execution by enhancing the responsibility decomposition , mechanism implementation by standardized completion,ideological implementation by education and training ,the implementation of standards by inspection and monitoring and finally form a team by enhanced communication .Results The technicalstandards of hospital infection management was implemented and the quality of hospital infection control was improved progressively .Conclusion It is an effective way to guarantee medical security to improve the execution of the technical standard of hospital infec -tion management .

The reproductive toxicity of environmental estrogens(EEs)has become an extensively studied subject in environmental science and reproductive medicine nowadays.Many epidemiology data and experimental evidences indicated that EEs may significantly cause the disorders of male sex differentiation such as microphallus,hypospadia,cryptorchidism and masculinization behavior disorder and so on.The mechanism includes estrogenic activity,anti-androgenic activity as well as other feedback regulation and signal transmission which influence hormones metabolism.

Objective To investigate the effects of children's mentality on mental symptoms during recovery period of ketamine anesthesia. Methods According to the mentality, forty-five children undergoing smalminor surgery were assigned into active group, stable group and fretful group with 15 cases each. Child was anaesthetized by ketamine alone. RP, BP, HR and SpO_2 were recorded at the end of operation, 5,10 and 30 min after operation. Meanwhile the expression, feeling, behavior, talking and activity were observed. Results RR, BP, HR in active group and fretful group were significantly higher than those in stable group. There was a significant difference in the expression, feeling, behavior, talking and activity among three groups. Conclusion Children's mentality has a significant effect on mental symptoms and hemodynamics during recovery period of ketamine anesthesia.

Nonaqueous capillary electrophoresis-chemiluminescence (NACE) method was developed for the determination of five phenolic environmental estrogens including bisphenol A (BPA),4-nonylphenol(4-NP) ,4-tert-octylphenol(4-tOP),4-tert-butylphenol(4-tBP) and tetrabromobisphenol A(TBBPA) in food packaging material. Target compounds in soaking solution of food packaging material samples were separated by NACE-CL after derivatization with 4-(4,5-diphenyl-lH-imidazol-2-yl) benzoyl chloride ( DIB-Cl) ,reacted with per-oxyoxalate chemiluminescence system,and then detected through magnifying light signal with photomultiplier. With 17β-E_2 as internal standard,qualitative and quantitative analysis of the target compounds were performed by relative migration time and the ratio of relative chemiluminescence intensity,respectively. Several influence factors on separation with NACE,such as the composition and proportion of organic solvent,the concentration of electrolyte,temperature surrounding of the capillary,the concentration of acetic acid and work voltage,were investigated. And the peroxyoxalate chemiluminescence system was optimized. Under these conditions,4-tBP,BPA,4-OP,4-NP and TBBPA were all separated and showed good linearities in the ranges of 0.0095-3.0 mg/L,0.0087-3.0 mg/L,0.0085 -3.0 mg/L,0.011 -3.0 mg/L,0.009-3.0 mg/L,respectively,with correlation coefficients over 0. 9947. The RSDs of relative migration time and relative chemiluminescence intensity ratio were 0.9% -3.0% and 6.5% - 8.6%. Recoveries for the spiked samples ranged from 86.7% to 98. 8%. Five common food packaging material samples were analyzed. The proposed method is simple and sensitive for the quantitative determination of phenolic environmental estrogens in food packaging material samples.

OBJECTIVE:To provide reference for clinical rational use of anti-epileptic drugs. METHODS:In this retrospective review,serum concentrations of anti-epileptic drugs in a total of 499 patients who were treated with anti-epileptic drugs (such as sodium phenytoin,phenobarbital,carbamazepine and valproate sodium) in our hospital during 2007 were analyzed statistically. RESULTS:Among the patients receiving one kind of anti-epileptic drugs,206 cases (61.49%) were within normal range in blood concentration versus only 45 cases (44.12%) for patients receiving combined drugs. In addition,the above four drugs (sodium phenytoin,phenobarbital,carbamazepine and valproate sodium) were detected in 59.68% of the patients who took Chinese medicines,and among them,3.23% were within normal range in blood concentration. CONCLUSION:Monitoring on serum concentrations of anti-epileptic drugs is conducive to a better control of therapeutic concentration. It is advisable to refrain from using anti-epileptic drugs in combination but to adopt individualized administration. In addition,great importance should be attached to whether there are chemo-synthetic drug components contained in Chinese medicine.

Objective To evaluate renal plication and nephropexy in the treatment of giant hydronephrosis. Methods 18 patients with giant hydronephrosis underwent relief of the obstruction as well as renal plication and nephropexy were reviewed. Results The patients have been followed up for three months to three years and their hydronephrosis improved a lot.The effected kidney showed some recovery of function on excretory urography. Conclusions Renal plication and nephropexy can promote recovery of renal anatomy and function after the relief of obstruction.

Objective To investigate the clinical application and value of spiral CT(SCT) in the diagnosis of renal cell carcinoma (RCC) with inferior vena cava (IVC) tumor thrombus. Methods Spiral CT scans were performed for 4 cases clinically confirmed as RCC with IVC tumor thrombus.The imaging findings were checked with post operative pathological findings. Results RCC with IVC tumor thrombus were assessed in all the 4 patients by SCT.The carcinoma stage and the type of the tumor thrombus were also determined.Operative findings have been the same as SCT. Conclusions SCT,a non traumatic and effective method,can make accurate diagnosis of RCC with IVC tumor thrombus,providing much more information for clinical diagnosis and treatment.

Objective To investigate the clinical singnificance of metalloproteinase(TIMP) 1 expression in human breast cancer.Methods The expression of TIMP 1 in paraffin embedded breast carcinoma specimens from 48 patients with primary invasive breast cancer who had undergone radical mastectomy or modified radical mastectomy from January of 1990 to Dcecember of 1996 were detected by the standard streptavidin biotin peroxides method,and its correlation to clinicopathologic featurea was analyzed. Ruslts Among 48 cases,the positive expression rate of TIMP 1 was 29%, TIMP 1 expression were higher in patients with higher histological grades than in those with lower ( P