Torque teno virus(TTV)is a nonenveloped virus containing a single-stranded,circular DNA genome of approximately 3.8kb.We completely synthesized the 3808 nucleotides of the TTV(SANBAN isolate)genome,which contains a hairpin structure and a GC-rich region.More than 100 overlapping oligonucleotides were chemically synthesized and assembled by polymerise chain assembly reaction(PCA),and the synthesis was completed with splicing by overlap extension(SOEing).This study establishes the methodological basis of the chemical synthesis of a viral genome for use as a live attenuated vaccine or gene therapy vector.

Congenital Abnormalities ; economics ; prevention & control ; Cost Control ; Humans ; Public Health Practice ; economics

Traditionally it has been widely accepted that our genes together with adult lifestyle factors determine our risk of developing noncommunicable diseases such as type 2 diabetes mellitus ,cardiovascular disease and obesity in lat-er life.Currently, there is substantial evidence that the early life environment plays a key role in determining our suscepti -ble to such diseases in later life .As an increased focus on the understanding of central insulin resistance , central nerve sys-tem plays an extremely important role in regulating the peripheral glucose homeostasis .Substantial studies have indicated that it may be regulated by epigenetic mechanisms .Epigenetics can be defined as the study of heritable changes in gene ex-pression that do not involve alterations in the DNA sequence .Epigenetic processes play a central role in regulating tissue specific gene expression and hence alterations in these processes can induce long -term changes in gene expression and me-tabolism which persist throughout the lifecourse .This review will focus on the epigenetic machanisms of central neuroregula-tion between early life environment and glucose metabolism in adulthood .

Objective:To compare the accuracy and safety between percutaneous kyphoplasty (PKP) positioning device and traditional positioning device which posit entry point of pedicle of vertebral arch by using the C-arm X-ray.Methods: 117 patients with spinal centrum compression fractures were divided into positioning device group (57 cases) and Kirschner wire group (60 cases) depended on the entry point marked by the preoperative C arm X-ray irradiation. To compare the excellence rate, operation time and exposure times between PKP positioning device and traditional device.Results: The positioning times of the PKP positioning device group and Kirschner wire group were (2.3±0.9)min and (6.8±1.3)min, respectively. The exposure times of the two group were 1.6 times and 4.7 times, respectively. And both of the two differences were statistical significant (t=21.062,t=18.521;P<0.05). The puncture success rates of the two group were 100%. And the excellence rates of them were 93% and 87%, respectively, and the difference also was statistical significant (x2=2.761,P>0.05).Conclusion: There are series of advantages in developed PKP positioning device, such as portable, easy and simple control, higher accuracy and safety, less positioning time and exposure times of C arm X-ray.

Objective: The current study investigate the immunological reaction state of Th1/Th2 cytokines expression in the peripheral blood mononuclear cells(PBMCs) from patients with lung cancer,and evaluate the effect of astragalus(AG) on it.Methods:The PBMCs from patients were divided into 4 groups:one group as pre-cultured group,and the other cells were cultured with 10%AG,20% AG and without AG (control group) ,respectively.The PBMC from 23 lung cancer patients as well as 19 healthy controls before and after the culture with AG were collected respectively. The Thl/Th2 cytokines expression were detected by RT-PCR considering IFN-r and IL-2 as Th1 cytokines and IL-4,IL-6,IL-10 as Th2 ones. Results:The results showed that the mRNA expression rate of IL-4,IL-6,IL-10 in PBMCs from lung cancer patients was obviously higher than that from the healthy controls( P

Nowadays the education of medical professional ethics has been obviously weakened.We can see that from some mainly expressions: There are many prejudices about medical ethics and the medical ethics education seems not very effective.But the medical professional ethics education is an important part of medical education,which directly affects the healthy development of medical and hygienic enterprise.And we must correct it,enhance it.We should inherit the good quarters of traditional medical ethics,reestablish the humanism medicine,promote virtue of medical personnel increasing,and establish the professional ethics of medical students before they become doctors.

Objective To study the effect of metoprolol combined with benazepril on cardiac structure and heart function in patients with heart failure and the treatment of hypertension in the elderly. Methods 120 cases of elderly hypertensive patients with heart failure from June 2011 to June 2015 were studied, according to the random number table method, they were randomly divided into combination group and control group.The two groups were given symptomatic and supportive therapy, the control group on the basis of conventional treatment were given antihypertensive drugs of benazepril; combination treatment group were given metoprolol on the basis of control group. After treatment, the clinical efficacy of the two groups were evaluated, the changes of cardiac structure in the two groups were recorded and analyzed before and after treatment, and the blood pressure and cardiac function were analyzed. Results The total effective rate of the combined group was 91.67%, the control group was 78.33%, there was significant difference between the two groups (χ2=4.1830, P=0.0408). After 6 months of treatment, the blood pressure, cardiac structure and 6min walking distance were significantly improved in the two groups (P<0.05). The above indicators in combination group were significantly better than those in the control group (P<0.05). The complication rate in combination group was 8.33%, 13.33% in the control group, there was no statistically significant between two groups (χ2=0.7764, P=0.3782). Conclusion Metoprolol combined with antihypertensive treatment can significantly improve the clinical efficacy of the treatment, improve the cardiac structure and function.

ObjectiveMatching pursuit algorithm(MAP),for its good parametric characterization,was applied in epileptic electroencephalography(EEG) to study time-frequency distribution.MethodsSimulation experiment of time-frequency analysis was carried out to verify the matching pursuit algorithm s superiority on frequency resolution and parametric characterization.Fourier transform,Wigner-Ville distribution and matching pursuit algorithm were applied to the time-frequency analysis on normal EEG and epileptic EEG to study epileptic discharge in the time-frequency plane and the results were compared.ResultsSimulation results showed that the matching pursuit algorithm obtained a better time-frequency distribution.Distributions of epileptic EEG and normal EEG had significant difference in time-frequency plane.ConclusionTime-frequency analysis based on matching pursuit can better reveal the EEG characteristics.

OBJECTIVE To study the phenomenon of hepatitis B surface antigen(HBsAg) and antibody(HBsAb) both positive in blood samples.METHODS Using electro-chemo-luminescence immune system E170 to detect HBsAg and HBsAb in clinical samples and retest the both positive cases by another two reagents for HBsAg and another three reagents for HBsAb.RESULTS All 7332 samples showed 140 both HBsAg and HBsAb positive(1.9%) and 73 of them were retested for HBsAb by another three reagents which gave altogether 24 positive results,which was not related to each other.Those gave higher HBsAb value by E170 showed higher positive rate by Abbott reagent.CONCLUSIONS HBsAg and HBsAb both positive results are not rare.It may be due to the interactions between the patient and the infected hepatitis B virus.Besides,the quality of reagents and correct lab operating procedures may also affect the test results.