1.Effect of glutaminase 1 inhibitor on carbon tetrachloride-induced liver fibrosis in mice
Chinese Journal of Biologicals 2024;37(2):183-187
Objective To investigate the effect of glutaminase 1(GLS1)specific inhibitor BPTES[bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl)ethyl sulfide]on the liver fibrosis in the mouse model of liver fibrosis induced by carbon tetrachloride(CCl4).Methods Male C57BL/6J mice were intraperitoneally injected with olive oil(control group),10%CCl4(10 μL/g,model group)or 10% CCl4(10 μL/g)+ BPTES(10 mg/kg,treatment group),with 10 mice in each group,two doses a week for four weeks to establish liver fibrosis model. Collagen deposition in mouse liver tissue was observed by Sirius red staining. The expression levels of actin alpha 2(Acta2),collagen typeⅠalpha 1(Col1a1)GLS1 and GLS1 protein were detected by qRT-PCR and immunohistochemical staining.Results Compared with the control group,the liver tissue of mice in the model group was generally enlarged,the surface was not smooth and granular,and the ratio of liver mass to tibia length significantly increased(t = 2. 979,P < 0. 05);The Sirius red positive area of collagen deposition increased signifi-cantly in the liver tissue of mice in the model group(t = 7. 661,P < 0. 01),the relative expression levels of Acta2 and Col1a1 significantly increased(t = 4. 335 and 5. 319,respectively,each P < 0. 01),and the mRNA and protein levels of GLS1 significantly increased(t = 5. 319 and 9. 725,respectively,each P < 0. 01). However,compared with the model group,the BPTES treatment group had a reduction in liver mass,a significant reduction in the Sirius red positive area of collagen deposition in liver tissue(t = 7. 427,P < 0. 01),and a significant reduction in the relative expressions of Atca2 and Col1a1(t = 3. 713 and 2. 628,respectively,each P < 0. 05).Conclusion Inhibition of GLS1activity can significantly improve the degree of liver fibrosis induced by CCl4,providing a new idea for the treatment of liver fibrosis.
2.Myofibroblast and hyperoxia lung injury.
Chinese Journal of Contemporary Pediatrics 2006;8(3):260-backcover
Animals
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Bronchopulmonary Dysplasia
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etiology
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Fibroblasts
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physiology
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Humans
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Hyperoxia
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pathology
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Infant, Newborn
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Lung
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embryology
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pathology
4.Penicillin skin test: status quo.
Chinese Journal of Pediatrics 2003;41(9):712-714
5.Glycemic control in diabetic patients during perioperative period
Chinese Journal of Endocrinology and Metabolism 2010;26(6):527-528
Today,more and more patients with diabetes are requiring surgical intervention.Not only the metabolic disturbance of carbohydrate will be aggravated by surgical stress,but also the risk of perioperative complications and mortality have been greatly increased in patients with diabetes.Therefore,a complete preoperative assessment and blood glucose management of preoperative,intraoperative,and postoperative periods are needed to get full assurance for diabetic patients to undergo surgery and to pass through the perioperative period smoothly.
6.Analysis of the clinical diagnosis and therapy and misdiagnosis for congenital middle ear cholesteatoma
Chinese Archives of Otolaryngology-Head and Neck Surgery 2016;23(3):150-152
[ABSTRACT]OBJECTIVETo investigate the methods of diagnosing and treating congenital middle ear cholesteatoma
and causes of misdiagnosis.METHODSThis was a retrospective review of 9 cases of congenital middle ear cholesteatoma in this hospital.RESULTS9 patients all received operative treatment and definite diagnosis was made by histopathologic investigation. The ossicular reconstruction was performed to 6 patients. All of the cases were followed-up for 3 months-2.5 years. A-B gap was 20 dB. After 1 year, no residual or recurrence of cholesteatoma was found in CT scan. All patients had been misdiagnosed. Misdiagnosis rate was 100%.CONCLUSIONCongenital middle ear cholesteatoma is a clinically rare and diagnosis is usually delayed in clinical practice due to the silent nature of the disease in its early stage. Preoperative diagnosis may be based on Levenson diagnostic criteria and CT imaging. Early surgical treatment can obtain a good effect of hearing reconstruction. To avoid misdiagnosis and incorrect treatment, radiological evaluation and careful local examination are really important, which may also avoid serious complications.
7.Analysis of the determination of NO and NOS in brain and myocardium of depression model rats
Chinese Journal of Behavioral Medicine and Brain Science 2009;18(7):589-590
Objective To study nitric oxide (NO) and nitric oxide synthase (NOS) in brain and myocar-dium of depression model rats and to explore the mechanism of brain and myocardium injuryed. Methods The de-pression model rats were produced by chronic mild unpredictable stress and separation. The behavior of rats were detected by open field test and sucrose consumption test. The contents of NO and NOS were determined with spec-trophotometric method. Results Compared with the normal control, the contents of NO [Brain ( 8.97±2.22 )μmol/g prot vs ( 1.86±1.28 )μmol/g prot; Myocardium (9.67±1.53) μmol/g prot vs (2.67±1.08)μmol/g prot] and NOS[Brain(9. 50±1.89) U/mg prot vs (2.31±0. 97) U/mg prot; Myocardium( 11.20±1.47) U/mg prot vs(2.53±0.97)U/mg prot] in brain and myocardium were significantly increased (P<0.01)of depression model rats. Conclusion The contents of NO and NOS increase significantly in brain and myocardi-um of depression model rats and it may induce the injury on brain and myecardium of them.
8.The Experience and Understand on Drug Dispensing by Automated Drug Dispensing Machine
China Pharmacy 2007;0(34):-
OBJECTIVE:To introduce how to use the automated drug dispensing machine to prepare drugs in single dose and to introduce our experience in the application of this machine. METHODS:The working principe, application context, and the functional advantage of the machine were introduced, and the problems encountered in the use of the machine was analyzed based on our experience, and the prospect of the machine was discussed. RESULTS: The machine enjoys the advantages of raising work efficiency, lowering dispensing error rate and bringing about convenience for patient to use, but it has its limitation in that it proposes higher requirement for software and hardware environment. CONCLUSION: The application of this automatic system will be more widespread with people’s increasing demand on medical care.
9.On the medical students' Humanism-Oriented Education In human's full scale development
Na AN ; Xiao-Bo WANG ;
Chinese Medical Ethics 1994;0(06):-
The theory of human's full scale development is an important content of the Marxism theory.It is the need of human's full scale development theory to strengthen the humanism - Oriented Education of the medical students.Under the situation of constructing the new medicine education pattern,we should consider thoroughly the necessity of strengthening the humanism - Oriented Education of the medical students and discusses profoundly the effective way to strengthen the humanism - Oriented Education of the medical students,thus improve the humanities quality of the medical students in the essence.
10.On Strengthening Education of Medical Students' Behavior Criterions Consciousness
Xiao-Bo WANG ; Na AN ;
Chinese Medical Ethics 1995;0(04):-
Contemporary medical college students' misconducts are so serious that it is urgent to strengthen their behavior criterions consciousness,for example,the sense of rules of law,codes of ethics,school disciplines,medical norm.Schools should do more to strengthen consciousness,make an effort to the daily management,actively guide students to participate in social practice.