The eighth edition of TNM staging system for head and neck squamous cell carcinoma has made several significant improvements on the basis of the previous one, which further increases its value in evaluating prog-nosis and guiding treatment strategies. However, results of some studies suggested that there's room for improvements in some respects. This article briefly summarized the major differences between the two editions, the remaining disad-vantages of the current system, the latest research progress and its potential development in the future.

ObjectiveTo investigate disease features and disposal of operating period on the senile female patients living in islands.MethodsTo analyze retrospectively clinical data of 84 senile female patients accepted gynecologic operation.ResultsAmong 84 cases, 39(46.23%) had gynecologic benign tumor, 24(28.57%) had hysteroptosis or anterior and posterior vaginal wall bulge, 15(17.86%) complicated hypertension, 7(8.33%) complicated diabetes and 5 (5.95%) complicated heart disease. Some cases showed hypotension during operating time, or suffered upper respiratory tract infection and urinary tract infection after operation.Conclusions Provided well preparation before operation and enhancing monitor during and after operation, old gynecologic patients can pass operation period safely.

Tuo-Fawas one of three internal treatment rules in traditional Chinese medicine (TCM) surgery. It had been widely used in the treatment of superficial suppurative diseases, visceral suppurative diseases and other diseases. This article was aimed to clarify the action mechanism ofTuo-Fa for carrying forward better services in the clinic. This article reviewed and recapitulated related literatures on experimental researches ofTuo-Fa in recent years. It mainly showed the research status on anti-infection, anti-tumor and anti-gastric ulcer withTuo-Fa and its prescriptions. It summarized the existed shortcomings, which was that experimental researches on the action mechanism ofTuo-Fa was still on the initial stage with a few references and obviously lagged behind clinical applications. However, some achievements were also made. It had initially revealed the action mechanism ofTuo-Fa in treatment of pyogenic infection. It also had research on action mechanism of anti-tumor and anti-gastric ulcer treatment. It developed new field for the clinical practice. Finally, the research on action mechanism ofTuo-Fa and future prospects were expressed.

Aged ; Aging ; Health Services for the Aged ; Humans

Humans ; Neurodegenerative Diseases ; prevention & control ; Quercetin ; therapeutic use

Cell Differentiation ; Culture Media ; Humans ; Interleukin-3 ; physiology ; Megakaryocytes ; cytology ; physiology ; Stem Cell Factor ; therapeutic use ; Thrombopoietin ; physiology

Objective:To construct recombinant retrovirus expressing human bone morphogenetic protein-7 gene BMP7 and to discuss its apoptosis-inducing activities and the mechanism in liver cancer cell line HepG2. Methods:BMP7 gene was amplified and reconstructed with retroviral plasmid pLP-LNCX by loxP homologous recombination,and then the plasmid pLP-LNCX-BMP7 (pLLBMP7) was transferred into packing cell line PT67 and the supernatant was collected to assay viral titer. MTT assay was adopted to observe HepG2 cells amplification. 48h after pLLBMP7 infection agarose electrophoresis and flow cytometry were used to verify apoptosis of tumor cells,and then the expression of BMP7,caspase-3 and bcl-2 proteins were detected by Western blotting. Results:Recombinant retrovirus pLLBMP7 was justified and transformed into PT67 package cell with supernatant viral titer amounted to 5?109 pfu/ml. In MTT assay retrovirus group had no evident difference from controls in cellular inhibition 72h later (35.1% vs. 5.3%,68.5% vs.18.3%,p

Objective: To observe the influence of trichostatin A (TSA) on the apoptosis of colonic cancer cell line HT-29 cells and expression of HIF-1α in HT-29 cells under hypoxia in vitro. Methods: HT-29 cells were treated with TSA(100, 200, 400, 600, 800 nmol/ L for 12, 24, 48, and 72 h. Cells in the control group were treated with the same volume of DMSO. MTT assay was used to investigate the survival of cells in each group. Annexin V and TUNEL were used to determine the apoptosis rate of HT-29 cells. RT-PCR and Western blotting were used to study the expression of HIF-1α mRNA and protein under hypoxia (37°C, 1% O2). Results: MTT assay demonstrated that cell viability decreased significantly in TSA-treated group compared to control group(P<0.05), and the inhibitory effect of TSA was in a dose- and time-dependent manner. Annexin V and TUNEL showed that TSA induced early apoptosis of HI-29 cells; the apoptosis rates in TSA treatment groups (200, 400, 600, and 800 nmol/L) were significantly higher than that of control group(P<0.05). TSA suppressed protein expression of HIF-1α under hypoxia in a dose-dependent manner but had no influence on mRNA expression of HIF-1α. Conclusion: TSA can induce apoptosis of HI-29 cells and inhibit their proliferation through suppressing expression of HIF-1α at protein level under hypoxia condition.

OBJECTIVE: To improve the oral bioavailability of glycyrrhizin by preparing glyeyrrhizin-sodium deoxycholate/phos-pholipid-mixed micelles (GL-SDC/PL-MMs). METHODS: GL-SDC/PL-MMs was prepared by a film dispersion method. In order toevaluate the property GL-SDC/PL-MMs comprehensively, physical and chemical properties determination, in situ intestinal absorption, and pharmacokinetics test were carried out. RESULTS: The average particle size of drug-loaded micelles prepared by film dispersion method was (82.99±7.5) nm and Zeta potential was (-32.23±1.05) mV. Compared with the control drug, glycyrrhizin loaded in SDC/PL-MMs significantly improved its in situ intestinal absorption. The oral bioavailability was markedly enhanced, indicated by the increase of ρmax to 77.26 μg · mL-1, which was 2.82 times of that of the control drug. CONCLUSION: SDC/PL-MMs is expected to be developed into a new drug delivery systems of GL.

Animals ; Calcium ; metabolism ; Cell Hypoxia ; Cells, Cultured ; Hippocampus ; cytology ; Neurons ; drug effects ; metabolism ; Rats ; Rats, Wistar ; omega-Conotoxins ; pharmacology