Objective To observe the depositing regions of the cerebral neurons containing A?_ 42 neurons and to study the effects of ginkgo leaf extracts and dioyridamole injection on the memory,the deposition of A?_ 42 in the brain of Alzheimer's disease(AD) model rats induced by aluminium chloride(AlCl_3).Methods The experimental AD model of rats was established by 30% AlCl_3.The rats of treatment group received ginkgo leaf extracts and dioyridamole injection for consecutive six months.Latent period and the intensity of electric shock was evaluated by a passive-avoidence test before and after administration respectively.The morphology and the number of A?_ 42 neurons of the model rats brain by light microscope observation as well as immnohistochemistry.Results (1)After treatment,latent period of the rats prolonged and the intensity of electric shock reduced as compared with AlCl_3 group.(2)The depositing regions of A?_ 42 were found mainly in pyramidal cell layer of CA_1 hippocampus formation and polymorphical cell layer of dentate gyrus,external main stratum of entorhinal region and Ⅲ,Ⅵ,Ⅴ cell layer of cortex(parietal,temporal).The color of A?_ 42 positive substance was brown or brown yellow in the cytoplasm and its dendrites or branches.Significant reducing of A?_ 42 neurons occurred in treatment group(hippocampus P0.05).Conclusion Ginkgo leaf extracts and dioyridamole injection can improve the AD model rats ability of learn and memorizing,which mechanisms are achived by preventing A? from depositing and clearing deposited A?.

Objective To study the effects of Qinglongyi polysaccharide on sialic acid (SA) content, Na+, K+-ATP and Ca2+, Mg2+-ATPase activity, and membrane potential in erythrocytes of S180 mice. Methods Using Kit to determine the SA content and Na+, K+-ATPase, Ca2+, Mg2+-ATPase activity, using flow cytometry to determine the membrane potential of erythrocytes of S180 mice. Results In the treated groups with three doses, it showed Qinglongyi polysaccharide could make the SA content increased (P

OBJECTIVE: To investigate the anti-inflammation effect of curcumin and its derivative in vivo. METHODS: Observations were made on the anti-inflammatory effect of curcumin and its derivatives in xylene-induced mouse ear edema and carrageenan-induced paw edema. The plasma T-AOC, MDA and tissue PGE2 levels were measured. RESULTS: Xylene-induced mouse ear edema and carrageenan-induced paw edema were improved by curcumin and its derivatives in different degrees (P < 0.05, P < 0.01), the plasma MDA levels were lowered, the T-AOC levels were increased, meanwhile PGE2 synthesis in the inflammatory tissues were inhibited (P < 0.05, P < 0.01). CONCLUSION: The anti-inflammatory activity of curcumin and its derivatives in mice may be related to increasing antioxidant capacity and inhibiting the PGE2 synthesis. Copyright 2012 by the Chinese Pharmaceutical Association.

Nucleotide binding oligomerization domain (NOD)-like receptor protein 3, NLRP3) inflammasomes regulate the secretion of caspase-1, interleukin-18 (IL-18), IL-1β, and other cytokines, and participates in aging. In recent years, it has been found that NLRP3 inflammasomes are abnormally activated in aging heart and vessels, and inhibition of NLRP3 inflammasomes can alleviate heart aging and vascular aging. This review summarizes the research of NLRP3 inflammasome in heart and vascular aging, and the related drugs to promote the discovery of the mechanism of NLRP3 inflammasome in heart and vascular aging and the development of related drugs.

Anemia, Aplastic ; genetics ; metabolism ; Bone Marrow Cells ; metabolism ; Case-Control Studies ; Cell Line ; Child ; Child, Preschool ; Female ; Fibroblast Growth Factor 2 ; metabolism ; Gene Expression Profiling ; Humans ; Male ; Mesenchymal Stromal Cells ; metabolism

Objective:To explore the relationship between wearing of functional appliance and systemic metabolism of PGE2 in the blood of growing rats.Methods:54 male Wistar rats aged 4-weeks were randomly divided into 3 groups(n =18):Daytime group,all-day group and control group.Self-guided functional appliance was made for mandible advancement of the rats in the 2 experimental groups.One week after appliance wearing,rats were sacrificed at the corresponding time points and blood in femoral artery was taken. PGE2 levels in the blood samples were measured,data were analysed by cosine equation.Results:Hypothesis was true in the control group(P ＜0.05),and was not true in both 2 experimental groups(P >0.05).Conclusion:PGE2 level in rat blood is circadian rhyth-mic,wearing of functional appliance made may disturb the rhythm.

Health Facilities ; Humans ; Medical Waste Disposal ; methods ; standards ; Public Health Practice ; standards

Cephalosporins ; adverse effects ; therapeutic use ; Humans ; Infant, Newborn ; Penicillins ; adverse effects ; therapeutic use ; Skin Tests

China ; Diabetes Mellitus ; diagnosis ; metabolism ; Glycated Hemoglobin A ; metabolism ; Humans

Hepatic Stellate Cells ; Humans ; Liver Cirrhosis ; diagnosis ; metabolism ; Signal Transduction