ObjectiveTo evaluate Trichosanthin(TCS) in treating ectopic pregnancy by com-paring to clinical efficacy and side effects between the intramuscular and intracervical adiministrationgroup. MethodsTCS was given to the patients by either the intracervical or the intramuscular route.Compare the clinical results. ResultsThere was a 90% cure rate in the intramuscular injection groupand a 94% cure rate in the intracervical injection group. Side effects were slighter in the intracervical in-jection group than intramuscular injection, especially in fever and its sustained time (P<0.05)。ConclusionsThe clinical efficacy was consistent in two groups but the side effects of the intra - cervicalgroup were slighter.

OBJECTIVESTo compare the gene expression profiles of acute promyelocytic leukemia cell line NB(4) before and after 12 hours of realgar treatment using cDNA microarray.

METHODSTwo cDNA probes were prepared through reverse transcription from mRNA of both untreated and realgar treated NB(4) cells. The probes were labeled with Cy3 and Cy5 fluorescence dyes individually, hybridized with cDNA microarray representing 1003 different human genes, and scanned for fluorescent intensity. The genes were screened through the analysis of the difference in two gene expression profiles.

RESULTSThe analysis of gene expression profiles indicates that 9 genes were up-regulated and 37 genes were down-regulated. Among the 9 up-regulated genes, 2 genes were involved in a proteasome degradation pathway. Some genes related to protein synthesis, signal transduction and cell receptors were down-regulated.

CONCLUSIONPSMC2 and PSMD1 genes may play an important role in the apoptosis and partial differentiation of NB(4) cells.

Arsenicals ; pharmacology ; Down-Regulation ; Gene Expression ; drug effects ; Humans ; Leukemia, Promyelocytic, Acute ; genetics ; Oligonucleotide Array Sequence Analysis ; Sulfides ; pharmacology ; Tumor Cells, Cultured ; Up-Regulation