[Objective] To investigate the effect of Ruangan Kangxian Prescription (RKP), a prescription mainly composed of Radix Salviae Miltiorrhizae, Radix Paeoniae Rubra, Catechu, Radix Stephaniae Tetrandrae and Polyporus, for rats liver fibrosis induced by carbon tetrachloride (CC14) and to explore its mechanism. [Methods] Rats with liver fibrosis were induced by CC14 and were randomized to RKP group, normal group, Biejia Ruangan Tablet (BRT) group and model group. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), hyaluronic acid (HA), laminin (LN) , procollagen Ⅲ (PCⅢ ) were detected; hepatic histopathology and immunohistochemical result of transforming growth factor ?1 (TGF ?1 ) were also examined. [ Results ] Death rate of rats was reduced; serological parameters of hepatic function and liver fibrosis were improved and liver fibrosis showed by immunohistochemical method, HE staining and Masson staining was inhibited in RKP and BRT groups (P 0.05). [ Conclusion ] RKP and BRT have the similar effect in preventing CC14-induced liver fibrosis in rats and its mechanism may be related to the counteraction of expression of TGF ?1 .

This study was aimed to select the best microwave extraction craft conditions of polysaccharide from Radix platycodonis. The composite scores of polysaccharide content and yield rate were used as evaluation indexes. By ex-amining the impact of microwave power, microwave time, extraction times and solid-liquid ratio on crafts, the best craft conditions were optimized by orthogonal test. The orthogonal test showed that when the microwave power was 1800 W, microwave time was 8 min, extraction times were 3, solid-liquid ratio was 1:15, the composite scores were the highest and the extraction craft conditions were the best. It was concluded that the crafts are efficient and stable. It had laid a foundation for the further study on polysaccharide from R adix p latyc odonis .

This study was aimed to investigate the anti-inflammatory, diuretic effect and acute toxicity of Herb of Lanceolate Sedge. The mice were randomly divided into 5 groups, which were the physiological saline group, aspirin group (0.05 g·kg-1), high dose (HD), middle dose (MD) and low dose (LD) of water extract of Herb of Lanceolate Sedge (10, 5, 2.5 g crude drug per kg) group. Intragastric administration of medication was given to mice once a day for 3 days. Auricular swelling induced by dimethyl benzene was used in the model establishment to calculate the swollen ear degree and the swollen inhibiting rate. And rats were randomly divided into 5 groups, which were the physiological saline group, aspirin group (0.05 g·kg-1), high dose (HD), middle dose (MD) and low dose (LD) of wa-ter extract of Herb of Lanceolate Sedge (10, 5, 2.5 g crude drug per kg) group. Intragastric administration of medi-cation was given to rats once a day for 5 days. Carrageenan induced paw edema was used in the model establishment to calculate the paw swollen rate. And rats were randomly divided into 5 groups, which were the physiological saline group, Furosemide tablets group (0.01 g·kg-1), high dose (HD), middle dose (MD) and low dose (LD) of water extract of Herb of Lanceolate Sedge (10, 5, 2.5 g crude drug per kg) group. Intragastric administration of medication was giv-en to rats once a day for 5 days. Water-loaded was used in the model establishment to calculate the 5-hour urine amount. The maximum tolerance dose (MTD) was used in the determination of the acute toxicity. The results showed that the three groups of HD, MD and LD of water extract of Herb of Lanceolate Sedge had obvious inhibiting effects on the swollen ear degree and the swollen inhibiting rate among mice, and the paw swollen rate of rats. It had signif-icant increasing effect on the 5-hour urine amount. The MTD of intragastric administration of water extract of Herb of Lanceolate Sedge was 640 g crude drug per kg for mice. It was concluded that Herb of Lanceolate Sedge dis-played obvious effects on anti-inflammatory and dieresis with low acute toxicity.

Objective To discuss the comparability of apolipoprotein results among different detection systems.Methods Three different kinds of biochemistry detection systems were used to detect apolipoprotein A and apolipoprotein B(apo-A,apo-B) concentration in 2 levels of Randox quality controls and 40 clinical sera according to EP9-A file. The collected data were dealt with statistical analysis.Results Analysis of variance showed apolipoprotein results from different control and patients sera had significant difference in different detection systems (P

Objective To discuss the comparability of blood glucose(Glu)results with different detection systems.Methods Six different kinds of biochemistry detection systems were used to detect plasma Glu concentrations at 2 levels of Randox quality controls and 48 clinical plasma according to EP9-A file.The collected data were treated with statistical analysis.Results Analysis of variance showed Glu results from different control and patients plasma had significant difference between various detection systems (P

Objective To discuss the comparability of total bibe acidl(TBA) results among different detection systems.Methods 3 different kinds of coagulation detection systems were used to detect TBA concentration in 2 levels of Randox quality controls and 45 clinical sera according to EP9-A file.The collected data were dealt with statistical analysis.Results The analysis of variance showed TBA resulls from different control and patients sera had significant diffefence in different detection systems (P

Renal function test items mainly include creatinine, urea, uric acid, cystatin C, urine-alb, urine protein, α1-microglobulin, β2-macroglobulin and retinol binding protein, etc.The standardization and harmonization of these renal function test items will contribute to clinical diagnosis and treatment.Therefore, many scholars are committed to the standardization of renal function tests and part of these testshave been standardized.But some test items, due to the complexity of the determination, the process of achieving standardization are still facing some problems and challenges.

Warfarin is a well-accepted drug used for preventing thromboembolic diseases. It is important to monitor the dosage of warfarin. Prothrombin time is one of the screening tests of extrinsic coagulation path,which is the first choice to monitor the oral use of warfarin. Standardized prothrombin time, namely international normalized ratio ( INR ) is used to adjust warfarin dosage in clinical practice. Many herbal medicines and foods may enhance the effect of warfarin therapy,and some of them may weaken this effect,which can result in severe clinical complications.

The 1,095 bp gene encoding peroxidase from Coprinus cinereus was synthesized and integrated into the genome of Pichia pastoris with a highly inducible alcohol oxidase. The recombinant CiP (rCiP) fused with the a-mating factor per-pro leader sequence derived from Saccharomyces cerevisiae was secreted into the culture medium and identified as the target protein by mass spectrometry, confirming that a C. cinereus peroxidase (CiP) was successfully expressed in P. pastoris. The endoplasmic reticulum oxidoreductase 1 (Ero1) and protein disulfide isomerase (PDI) were co-expressed with rCiP separately and simultaneously. Compared with the wild type, overexpression of PDI and Erol-PDI increaseed Cip activity in 2.43 and 2.6 fold and their activity reached 316 U/mL and 340 U/mL respectively. The strains co-expressed with Erol-PDI was used to high density fermentation, and their activity reached 3,379 U/mL, which was higher than previously reported of 1,200 U/mL.
Coprinus ; enzymology ; Culture Media ; Cytoplasm ; Fermentation ; Glycoproteins ; metabolism ; Mass Spectrometry ; Mating Factor ; Oxidoreductases Acting on Sulfur Group Donors ; metabolism ; Peptides ; Peroxidases ; biosynthesis ; Pichia ; metabolism ; Protein Disulfide-Isomerases ; metabolism ; Protein Folding ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae Proteins ; metabolism

Objective To construct the recombinant plasmid pET32a-AKT1 and express human AKT1 protein using prokaryotic expression system .Methods Reverse transcriptase-polymerase chain reaction(RT-PCR) was employed to amplify the gene AKT1 in coding region and integrated it with pET 32a plasmid ,following by transforming it into Escherichia coli DH5α and prokaryotic strains BL21(DE3) .Isopropyl-beta-D-thiogalactopyranoside(IPTG) was adopted to induce its expression .Sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE ) and Western-blot were used for protein identification .Results Complete fusion of target gene and plasmid was observed .The recombinant plasmid pET32a-AKT1 was successfully transferred into the strain DE3 . After IPTG induction ,protein with relative molecular mass 70 000 was expressed by DE3 .Conclusion The recombinant plasmid pET32a-AKT1 is constructed successfully and AKT 1 protein is completely and efficiently expressed by prokaryotic strain DE 3 .