Objective To investigate the effect of stellate ganglion block(SGB) on the plasma concentration of noradrenaline(NE) in rabbits suffering from acute pain and the possible mechanism.Methods Fourteen healthy rabbits of both sexes weighing 2.5 2.8 kg were anesthetized with 20% urethane 1 g?kg -1 . Spontanous breathing was maintained. Right stellate ganglion was exposed aseptically. An epidural catheter was fixed with one end placed close to stellate ganglion and the other end outside the neck through a hole on the skin for administration of drugs. One week later 3% formalin 0.2ml was injected subcutaneously into plantar region of the right paw. Pain response was observed . 60 min after formalin injection 0.25% bupivacaine 0.5ml was injected through catheter (bupivacaine group n=7) while in control group (n=7) normal saline 0.5ml was injected. The effect of SGB was confirmed by ptosis and miosis. Blood samples were taken from edge vein of the ear 10 min before (T 0) and 10(T 1), 30(T 2), 50min(T 3) after subcutaneous injection of formalin and 10min(T 4), 30min(T 5), 50min(T 6) after bupivacaine or normal saline injection for determination of plasma NE concentration by radioimmunoassay.Results Plasma NE concentration increased significantly after subcutaneous formalin injection and peaked at T 1,then decreased slightly at T 2 and T 3. In group B, plasma NE concentration decreased significantly after bupivacaine injection, while in control group there was no significantly change in plasma NE concentration after normal saline injection.Conclusions SGB reduces the increased plasma NE concentration in rabbits suffering from acute pain. This may be the possible mechanism of analgesia provided by SGB.

Objective To investigate the effect of stellate ganglion block (SGB) on substance P of spinal cord in rabbits following formalin stimulation and the possible mechanism. Methods Rabbits of both sexes weighting 2.5-3.0 kg were anesthetized with 20% urethane 1 g?kg-1 . Spontanous breathing was maintained. Right stellate ganglion was exposed aseptically. An epidural catheter was fixed with one end placed closed to stellate ganglion and the other end outside the neck through a hole on the skin for administration of drugs. One week later, nineteen healthy rabbits were randomly divided into three groups: sham operation group (group A, n = 5), SGB group (group B, n=7) and control group (group C, n = 7) . In group B and group C, 3 % formalin 0.2 ml was injected subcutaneously into plantar region of the right paw. Pain response was observed. 1h after formalin injected, 0.25% bupivacaine 0.5 ml was injected through the catheter in group B while normal saline 0.5 ml was injected in group C. The effect of SGB was confirmed by ptosis and miosis. 1h after using of bupivacaine or normal saline, rabbits were deeply anesthetized and chest was opened, 1 000 ml of normal saline was infused via left ventricle then followed by 2000-2500 ml of 4% paraformaldehyde in 0.1mol/L phosphate buffer pH7.4 infusion for fixation of tissue, 60 min later spinal cord of cervical 6-8(C6-8) and thoracic 6-8(T6-8 ) were removed. In group A the spinal cord were extracted similar to the other groups. The content of SP in spinal cord use immumohistochemistry technique of strept avidin biotin enzyme complex (SABC) to display. Results Immunoreaction of SP were distributed mainly in lamina Ⅰ and Ⅱ of dorsal horn of spinal cord. Compared with group A, the optical density(OD) of SP in cervical slices were decreased significantly in group B and group C(P 0.05 ). Conclusions The content of SP in spinal cord decreased significantly after subcutaneous injection of formalin in rabbits. SGB can reverse the phenomena partly and increase the content of SP of cervical spinal cord in rabbits following formalin stimulation. This may be the possible mechanism of analgesia provided by SGB.

Objective To investigate the effect of ketamine on spatial learning and memory in a rat model of acute pain produced by an incision in plantar area. Methods Seventy-two 3-month old male SD rats weighing 200-250g were randomly divided into 3 groups: (1) control group (C) received no plantar incision (n = 24); (2) acute pain group (M) received an incision in the sole of the hindpaw according to the method described by Brennan (n = 24) and (3) ketamine group (K) received plantar incision and intraperitoneal ketamine 10 mg?kg-1 every day for 7 days starting from the day when plantar incision was made ( n = 24). In group C and M normal saline (NS) was given i.p. instead of ketamine. The three groups were further divided into 2 subgroups according to the time when Morris water maze (MWM) testing was started-1 week (C1, M1,K1) or 3 weeks (C3, M3, K3) after plantar incision. MWM test was performed 4 times a day for 6 consecutive days. The latent periods and swimming distances were recorded automatically by MWM monitoring system. Six days after the last MWM testing the animals were anesthetized and killed. The hippocampus was removed for microscopic examination. Results (1) MWM test: From the 1st to the 6th day of testing, the latent period and swimming distance in group K1 were significantly longer than those in group C1 and M1 (P

To study the bioactive polypeptides included in Bufo skin and its secretions the plasmid skin cDNA library of adult Japanese toad Bufo japonicus formosus was prepared. The pSD64TR has been used as the vector and the cloning sites are Xho I and EcoR I. To screen cDNAs encoding bioactive components, the plasmid cDNA library was transformed into E. coli DH5 competent cells, and positive colonies were screened by colony PCR (polymerase chain reaction). The suspension of a single colony in LB medium was used as the template, SP6 (the upstream primer of the plasmid cDNA library) and a primer with Xho I site and polyT were used as the primers. As the result, 465 positive colonies out of 1 344 were obtained and their plasmid were collected and sequenced. By homologous analysis, it was found that one of the cDNAs encoding a peptide with high homolog with transgelin-2, which was registered in GenBank (accession number: JX197456), and it was indicated as a partial cDNA sequence with a deletion at the 5' end. The transcript is 997 bp consisting of 31 bp 5', 618 bp 3' untranslated region (UTR) and an open reading frame (ORF) of 348 bp encoding a polypeptide of 115 amino acids. In the putative protein product, there is a calponin homology domain, two cysteine residues for a disulfide bond and three a-helix domains, and five potential phosphorylation sites. The homologous analysis indicates 90% similarity with Xenopus (Silurana) tropicalis and 89% with Xenopus laevis, and 71%-85% with other species.

Objective:To establish a highly sensitive method by immuno PCR for measurement of GH in Urine.Methods:Sandwich ELISA was operated in PCR tubes which treated with glutarakdehyde,and PCR was tested in the same PCR tube.The PCR production were electrophoresis in Agarose gel and Scan for gel.Results:The results show that ELISA could be correctly reacted in PCR tubes which treated with glutarakdehyde,more than 0.1 pg/ml GH could be detected.Results of Scan showed that the GH values in urine overnight have significantly deference between 30 normal children and 10 GHD patients which were diagnosed by GH provocative test.Conclusion:The sensitivity of Immuno PCR is 10 3～10 4 times higher than that of ELISA,Immuno PCR could be used in detection of GH in urine of children.

Objective To investigate the difference of expression and distribution of Toll-like receptor 4 (TLR4)between acquired immunodeficiency syndrome (AIDS)-related Kaposi sarcoma (AIDS-KS)and classic Kaposi sarcoma (KS).Methods The subjects were collected from The First Teaching Hospital of Xinjiang Medical University from January 2010 to January 2012,including 11 patients with AIDS-KS,7 patients with classic KS,7 human immunodeficiency virus (HIV)infective patients without KS and 7 healthy volunteers.Clinical data and biopsy specimens were collected.The expression of TLR4 was detected by immunohistochemical method.Statistical analysis of mean between two groups was conducted by using t test.Statistical analysis of ranked data was conducted by using U test.Comparison between groups was analyzed with one-way ANOVA.Results The expressions of TLR4 were both strongly positive in patients with AIDS-KS and classic KS,and mainly in alien vascular endothelial cells (VEC)and tumor cells.TLR4 was located on cell membrane and cytoplasm,and also in the nucleus. TLR4 was rarely expressed in the skin tissue in HIV infective patients without KS and healthy volunteers. The expressions of TLR4 in AIDS-KS,classic KS,HIV infective patients and healthy volunteers were 0.242±0.443,0.208±0.059,0.072±0.042 and 0.065 ±0.031 ,respectively (F =33.70,P <0.01 ). The expressions of TLR4 were lower in HIV infective (AIDS ) patients without KS and healthy volunteers.The expression level of TLR4 was significantly higher in KS patients compared with non-KS patients (t=4.097,P =0.003).However,TLR4 expression had no significant difference between AIDS-KS and classic KS patients (t =0.480,P =0.638).The expression of TLR4 was significantly higher in AIDS-KS patients compared with HIV infective patients (t=2.900,P =0.010).Conclusions The study identifies that TLR4 is highly expressed in patients with AIDS-KS or classic KS.It is suggested that TLR4 may play an important role in occurrence and development of KS.

Objective To compare the therapeutic efficacies of fire-needle acupuncture and Halometasone cream in treating sub-acute and chronic eczema. Method Totally 114 patients were divided into a treatment group and a control group. The treatment group was intervened by using fire-needle acupuncture, and the control group was by Halometasone cream. Itchy score and Eczema Area and Severity Index (EASI) were used for comparison before and after intervention. Result There were significant differences in comparing the markedly-effective rate and total effective rate between the fire-needle acupuncture group and the Halometasone control group according to the itchy score (P＜0.01). There were no significant differences in comparing the markedly-effective rate and total effective rate between the two groups according to EASI (P>0.05). Conclusion Fire-needle acupuncture is superior to Halometasone cream for acute and chronic eczema in relieving itch, action time and effect-sustaining duration.

ObjectiveTo study the correlation between the etiology and clinical features of erythroderma.MethodsThe clinical data on 182 patients with erythroderma were retrospectively collected and analyzed.ResultsThe male-to-female ratio was 2.8 ∶ 1 and the average age at onset was 58.6 ± 14.6 years.Of the 182 cases,135 (74.2％) were due to pre-existing dermatoses,14 (7.7％) to drug reaction,8 (4.4％) to malignancies,while 25(13.7％) had no obvious precipitating factors.The most frequent triggering factor was systemic consumption of drugs(52 patients,28.6％ ),and glucocorticosteroid was the most prevalent causative drug.Seventy-six patients were followed up,recurrence was observed in 14 patients but not in 58 patients,and 5 patients died,2 patients with idiopathic erythroderma were finally diagnosed with mycosis fungoides (MF)after multiple skin biopsies.ConclusionsPre-existing dermatoses are the most frequent cause of erythroderma.Idiopathic erythroderma is liable to relapse,possibly associated with malignancies,and should be closely followed up.

Objective:To introduce a new technique of double eyelid blepharoplasty by manipulating the relocation and reconnection of aponeurotic flap with tarsus (Bridge Technique), which can achieve physiological, dynamic, natural-looking and minimal-scar double eyelids.Methods:From September 2018 to January 2020, subjects from the Han ethnicity who received double eyelid blepharoplasty using our "Bridge Technique" in the Department of Plastic and Reconstructive Surgery, the Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, were included. The overall aesthetic outcomes were comprehensively graded as good, moderate and poor both by doctors and patients regarding on palpebral fissure, the smoothness of eyelid margin and double eyelid, evenness of incision, symmetry and relapse rate, respectively.Results:151 subjects, including 127 female and 24 male, were included. Their age ranged from 18-41 years old (mean, 27.1 years). At the follow-up of 6-month post-surgery, 90.7% (137 cases), 7.3% (11 cases) and 2.0% (3 cases) of their aesthetic outcomes were graded as good, moderate and poor by three single-blinded doctors, respectively. 86.1% (130 cases), 11.9% (18 cases) and 2.0% (3 cases) were graded as good, moderate and poor by the patients themselves. The overall follow-up period ranged from 6-18 months (mean, 12.7 months). The long-term outcomes were achieved to be stable without any complications.Conclusions:The "Bridge Technique" , which is the tarsal-fixation with aponeurotic flap linkage in blepharoplasty, has been proven as an effective technique for creating dynamic, stable, natural-looking and physiological double eyelids. In addition, it can also effectively avoid unsatisfactory corneal exposure rate which might be caused by the mild ptosis with poor levator function.

Objective To investigate the conversion rate, organ procurement rate and influencing factors of organ donation in a single center from Fujian province. Methods Baseline data of 182 potential organ donors of Fujian Medical University Union Hospital from November 2018 to June 2021 were retrospectively analyzed. The conversion rate of organ donation, baseline data of successful organ donors and the causes of failure of organ donors were identified. The organ procurement rate and the influencing factors of the number of organ donations were analyzed. Results Among 182 potential organ donors, 46 cases were successful organ donors with a conversion rate of 25.3%. In addition, 136 cases failed to donate organ. The main causes included disagreement from family members (58.1%), insufficient evaluation time (24.3%) and ineligible for donation criteria (17.6%). A total of 212 large organs and tissues were donated by 46 organ donors, including 88 kidneys, 42 livers, 15 lungs, 19 hearts and 48 corneas, with 4.6 large organs and tissues, and 3.6 large organs for each donor. Age, sex, native place, organ donation area and blood type were the influencing factors of the number of large organ donations. Organ donation area was the influencing factor of the number of tissue donations. Conclusions The conversion rate of organ donation is relatively low in a single center from Fujian province. Disagreement from family members is the main cause. Suitable potential organ donors should be selected for organ donation to improve the conversion rate and organ procurement rate of organ donation.