Objective To investigate the therapeutic effect of continuous intravenous pralidoxime chloride infusion in acute organophosphorus pesticide poisoning(AOPP).Methods The patients with severe AOPP were randomly divided into 3 groups:(1)group 1(n =51)received a bolus injection of pralidoxime chloride 2.Og followed by continuous intravenous infusion at 0.25 g/h.(2)group 2(n = 51)received a bolus injection of pralidoxime 2.Og followed by continuous intravenous infusion at 0.5g/h.(3)group 3(n = 50)received intravenous drip of pralidoxime 2.Og for 3 times a day.Efficacy was compared among 3 groups on the basis of time to reach atropinization,recovery of cholinesterase activity .cumulative amount of atropine,incidence of recurrence of pesticide poisoning,intermediate syndrome,and hospitalization days,etc.Results Efficacy in patients receiving continuous intravenous therapy was significantly different from the third group.But there was no significant difference in efficacy between the first and second groups.Conclusion The patients with AOPP can be effectively treated by a loading dose followed with continous intravenous pralidoxime chloride infusion.

With the development of medical technology and the progress of society, patients requiring oral cavity cosmetology are getting more and more and the cosmetic medical science of oral cavity is also developing gradually. This article explains the ethical principles that should be followed while treating and the quality that the doctor should acquire in terms of ethics.

Objective To evaluate the clinical value of co-observation of the expression of MAGE-A3 gene and MDR1 gene on esti-mating the curative effect in non M3-subtype acute leukemia. Methods Expressions of MAGE-A3 and MDRI were measured in 77 patients with non M3-subtype acute leukemia by RT-PCR method. Clinical observation was done to estimate the relationship between the genes with curative effect in non M3-subtype acute leukemia. Results Expression of MAGE-A3 and MDRI gene were 50. 6% and 23. 3% in non M3-subtype acute leukemia patients. Positive expression of MDRI in MAGE-A3-positive and negative patients were 46. 2% and 13. 2% (P < 0.01). The complete remission rate in MAGE-A3 negative and positive patients were 86. 8% and 64. I% (P <0. 05). Complete remission (CR) rate in MDR1 negative and positive patients was 83.3% and 56. 5% (P <0. 05). Complete remission rate were 87.9% and 55.6% in beth negative and both positive expression of MAGE-A3 and MDR1 (P < 0. 05). Conclusion The patients of positive expression of MAGE-A3 in non M3-subtype AL had higher expression of MDR1. The patients with negative expression of beth MAGE-A3 and MDR1 had higher CR rate than that in both positive patients. These researches indicated that eo-observatian of the expression of MAGE-A3 and MDR1 can predict the curative effect in non M3-subtype AL.

Objective To explore the value of two dimensional and color Doppler flow imaging(2D CDFI)for the diagnosis and differentiation of the carotid body tumor.Methods Retrospective analysis was made on 12 patients with carotid body tumor examined by 2D CDFI,and differentiations were conducted between several similar diseases.All the cases were confirmed by surgical pathology.Results The carotid body tumor in 2D CDFI was shown as follows: Low echoic substantial masses were found in the carotid branch with clearly boundary,and or partly outline.Most in small size were located at the branch of common carotid artery,so the interval between external and internal carotid arteries was enlarged.The masses in big size always grew around common cartid artery internal carotid artery and external carotid artery.More color flow signals were detected in the masses,and most were artery flow.The relationship between the mass and the carotid was shown clearly by 2D CDFI.Conclusions 2D CDFI is a valuable and practical method in diagnosis of carotid body tumors.

BACKGROUND: Comparing with diabetic nephropathy and diabetic retinopathy, diabetic cardiomyopathy is put forward exceeded later and the research about it was not enough. It is difficult to detect diabetic cardiomyopathy in the earlier period so that it has usually been neglected.OBJECTIVE: To evaluate features of dysfunction of left ventricle in early diabetic cardiomyopathy by Doppler tissue imaging.DESIGN: Completely randomized and controlled experiment.SETTING: Ultrasound Department of General Hospital of Chengdu Military Area Command of Chinese PLA.MATERIALS: The experiment was carried out at Ultrasound Department of General Hospital of Chengdu Military Area Command of Chinese PLA from March to June 2002. Totally 56 New Zealand rabbits of either gender were selected.METHODS: ① Ten New Zealand rabbits was pulled out randomly from 56 as control group. The rest of 46 New Zealand rabbits were replicated diabetes mellitus by Streptozotocin (STZ), rabbits were absolute diet 18 hours and injected intravenously to ear marginal vein with 65 mg/kg of STZ. Then these rabbits were randomly divided into 4 groups as 2, 4, 6, 8th experimental group, with 10 rabbits in each group. ② Digit ultrasonic cardiogram equipment was used to examined interventricular motion of mitral annulus at 2nd,4th, 6th and 8th week in model groups and comparison group. The parameters such as systolic peak velocity (Sa), preejection period (PEP), local ejection time (ET), systolic mean velocity (Vm) of four various portions in mitral annulus and diastole early period velocity (Ea), diastole later period velocity (Aa), Ea/Aa were determined. ③ SPSS 11.0 was used for statistical analysis,one-factor analysis was adopted to compare difference of groups.MAIN OUTCOME MEASURES: The motion of lateral wall and posterior interventricular septum at mitral annulus were estimated by PW-DTI.RESULTS: Among 56 rabbits, 50 entered the final analysis and other 6 were lost because of failure modeling. Diastole dysfunction of left ventricle was discovered in STZ-inducod diabetic rabbits detected by tissue Doppler imaging at the 4th week. It showed that not only the tite of early period of diastole peak velocity (Ea) in mitral ring lateral wall and post-interval of left ventricle was reduced, but also the tite of Ea/Aa was reduced notably (P ＜ 0.05). Systolic dysfunction of left ventricle was discovered in STZ-in-duced diabetic rabbits detected by tissue Doppler imaging at the 6th week.It showed that local contraction peak velocity (Sa) of lateral wall and postseptum of mitral ring was decreased strikingly (P ＜ 0.05). Mean systole velocity of four various parts of mitral ring (Vm) was reduced at the 6th week (P ＜ 0.05). Pre-ejection period time (PEP) at post-septum and ejection time (ET) was elongated at lateral wall at the 8th week (P ＜ 0.05).CONCLUSION: ① Diastolic dysfunction of left ventricle is characterized by decrease of early period of diastole peak velocity and the tite of Ea/Aa.② Systolic dysfunction of diabetic cardiomyopathy is characterized by decrease of local contraction peak velocity and elongation of pre-ejection period time and ejection time.

Objective:To preliminarily investigate the effect of circIGF2BP3 on autophagy in photoaged dermal fibroblasts.Methods:Human dermal fibroblasts were isolated from circumcised foreskin tissues from 6 children in the Department of Urological Surgery, Third Affiliated Hospital of Sun Yat-sen University. An ultraviolet A (UVA) -induced photoaged human dermal fibroblast model (UVA radiation group) was established by repeated UVA radiation at a dose of 10 J/cm 2 for 14 consecutive days, and human dermal fibroblasts receiving no treatment served as control group. The photoaged cell model was verified by β-galactosidase staining, Western blot analysis for determining P21 protein expression, and cell counting kit-8 (CCK8) assay for evaluating cell viability. Moreover, Western blot analysis was performed to determine the protein expression of autophagy-related proteins P62, microtubule-associated protein 1 light chain 3 (LC3) -Ⅰand LC3-Ⅱ in photoaged human dermal fibroblasts, and real-time quantitative RCR (qRT-PCR) to verify the differential expression of circIGF2BP3 between photoaged and normal human dermal fibroblasts. Furthermore, circIGF2BP3 was biologically annotated. Some cultured primary human dermal fibroblasts were divided into 4 groups: empty vector group transfected with an empty vector, UVA + empty vector group transfected with an empty vector followed by repeated UVA radiation, circIGF2BP3 group transfected with a circIGF2BP3-overexpressing lentiviral vector, UVA + circIGF2BP3 group transfected with a circIGF2BP3-overexpressing lentiviral vector followed by repeated UVA radiation. Western blot analysis was performed to determine the expression of autophagy-related proteins. Statistical analysis was carried out by using t test, one-way analysis of variance and least significant difference- t test. Results:Compared with the control group, the UVA radiation group showed significantly increased proportions of β-galactosidase-positive cells (61.33% ± 5.78% vs. 6.37% ± 0.32%, t = 9.49, P < 0.01) and P21 expression (1.25 ± 0.03 vs. 1.00 ± 0.05, t = 4.26, P < 0.05), but significantly decreased cell viability (74.33% ± 3.48% vs. 100%, t = 7.38, P < 0.01). Moreover, the P62 expression and LC3-Ⅱ/Ⅰ ratio were significantly higher in the UVA radiation group than in the control group (both P < 0.05). The relative expression of circIGF2BP3 was 0.72 ± 0.04 in the photoaged human dermal fibroblasts, which was significantly lower than that in the normal human dermal fibroblasts (1.00 ± 0.03, t = 5.46, P < 0.01). The P62 expression and LC3-Ⅱ/Ⅰ ratio were significantly lower in the circIGF2BP3 group (0.60 ± 0.01, 0.71 ± 0.01, respectively) than in the empty vector group (1.00 ± 0.02, 1.00 ± 0.01, t = 16.25, 2.75, P < 0.01, < 0.05, respectively), and lower in the UVA + circIGF2BP3 group (1.05 ± 0.02, 2.04 ± 0.05, respectively) than in the UVA + empty vector group (1.31 ± 0.02, 2.72 ± 0.14, t = 10.493, 6.472, respectively, both P < 0.01) . Conclusion:circIGF2BP3 can regulate autophagy in UVA-induced photoaged dermal fibroblasts, which provides a new potential therapeutic target for the prevention and treatment of skin photoaging.