The reputation of a hospital is a non-visible resource of great importance. Strengthening the management of hospital reputation is an objective requirement put forward by the buyer's market under the market economic system and by the features of the medical services industry, for enhancement of a hospital's reputation can meet the emotional needs of patients. Thus strengthening the managenment of hospital reputation is of great importance. The measures include ①working out goals of reputation management; ②laying equal emphasis on hospital reputation and ethics in decision making; ③increasing investment in hospital reputation; ④strengthening the dissemination of hospital reputation and ⑤mobilizing the entire hospital staff in creating an image of the hospital with a sound reputation.

The reputation of a hospital is a non-visible resource of great importance. Strengthening the management of hospital reputation is an objective objective Put forward by the buyer's market under the market economic system and by the features of the medical services industry, for enhancement of a hospital's reputation can meet the emotional needs of patients. Thus strengthening the management of hospital reputation is of great importance. The measures include ①working out goals of reputation management; ②laving equal emphasis on hospital reputation and ethics in decision making; ③increasing investment in hospital reputation; ④shengthening the dissemination of hospital reputation and ⑤mobilizing the entire hospital staff in creating an image of the hospital with a sound reputation.

Doctor-patient right conflict should be noticed at present. Hospital administrators should strengthen ''sense of patients right" and get to the consistency of economic administrative aim and patients right protection while making choices in administration. They should realise the combination between rights and duties of health staff and reach the hermonization of society, patients and hospital beneficts.

To produce the reverse transcriptase of moloney murine leukemia virus (MMLV-RT) through gene recombination, MMLV-rt gene was amplified by polymerase chain reaction (PCR) with specifically designed primers bearing restriction enzyme sites. Five mutation sites increasing the solution of the target protein were introduced through Site-directed mutation. After verification by sequencing, the gene was cloned into the expression vector pET15b to construct the recombinant plasmid pET15b-MMLV-rt. Purified MMLV-RT was obtained by affinity chromatography (Ni3+-NTA beads). Molecular weight and purity of MMLV-RT were analyzed with SDS-PAGE. Enzyme activity was characterized with RT-PCR. We successfully constructed the recombinant plasmid pET15b-MMLV-rt and obtained the MMLV-RT fusion protein with 6His on the N-terminus. Recombinant protein was purified through Ni3+-NTA beads based affinity chromatography, the purity of which was 96%. The Activity of the enzyme was high. MMLV-RT of 96% purity was obtained with the prokaryotic expression technique, which serves as the basis for mass production of this enzyme.
Animals ; Mice ; Moloney murine leukemia virus ; enzymology ; genetics ; RNA-Directed DNA Polymerase ; biosynthesis ; genetics ; Recombinant Fusion Proteins ; biosynthesis ; genetics ; metabolism ; Recombination, Genetic

Objective To observe the cardiac function in acute brain injury patients(ABI)and the relationship between ABI and plasma neuropeptideY(NPY),and to inspect the mechanism and find the evidences for preventing cardiac impairment caused by ABI. Methods 89 patients with acute brain injury within 24 hours after the injury were divided into severe group(n =47)and mild group(n = 42)according to Glasgow Coma Scale(GCS),and 35 normal healthy adults were selected as control group.In 24 hours and 72 hours after the brain injury,all patients were examined with echocardiography to observe cardiac structure,Doppler blood flow velocity and cardiac function,and in the same time the plasma NPY were determined by radioimmunoassay.Then the results were compared with controls. Results The parameters of cardiac function such as EF、 SV.AV、CO、CI had statistical change in 24 hours and 72hours after the brain injury between severe ABI group and mild ABI group,and it also had statistical change between severe ABI group and control group(all P <0.05),but no statistical change between mild ABI group and control group(all P ＜0.05).The level of plasma NPY in ABI patients was significantly higher than that before injury,there was statistically different change between severe ABI group and mild ABI group,and it also had statistical change between severe ABI group and control group(all P＜0.05).The parameters of cardiac function was negatively correlated with the rise of plasma NPY by pearson correlation analysis(EF:r =- 0.79,P <0.01; SV:r =- 0.71,P <0.01;AV:r=-0.67,P ＜0.01 ;E/A:r =-0.63,all P ＜0.01)and(CO:r =- 0.32,P ＜0.05;CI:r =-0.35,all P ＜0.05). Conclusion The parameters of cardiac function were significantly decreased in the patients with acute brain injury,and it was closely related with the level of plasma NPY.