Objective To make sure if there is single nucleotide polymorphisms(SNP) of c135 in RET proto-oncogene in Han ethnic group of Shaan Xi,China and investigate the relationship between Hirschsprung disease(HSCR) and SNP of RET proto-oncogene c135.Methods A total of 40 blood samples were collected from healthy donors through cluster sampling in Xi'an,Shaan Xi province.Sixteen tissues in aganglionic segments of HSCR were studied(they were all sporadic in Han ethnic group of Shaan Xi province of China and diagnosed by pathology).DNA was isolated from above samples and PCR amplified.The fragment of products were 300 case pairs(bp) which located in RET exon2 including c135.All of fragments were purified and directly sequenced and analyzed by related software and statistical methods.Results There really existed the single nucleotide polymorphisms of c135 A/G of RET in controls.A,G allele frequency was 0.475 and 0.525,respectively,and there was no distinctive dominant genotype.In patient group,A,G allele frequency was 0.844 and 0.156,respectively.c135A genotype was dominant in patients.There was significant statistical difference between patients and controls in RET c135A/G(PA.There is relationship between the disease and the c135 A genotype of RET.c135A genotype in the RET proto-oncogene are over-expressed in patients with Hirschsprung disease.

Objective To investigate the curative effect of endovascular treatment for inferior vena cava syndrome(IVCS).Methods A total of 17 cases of inferior vena cava syndrome was treated by endovascular dilatation and self-expandable metallic stent placement between June 2002 and November 2004.Routine anticoagulation therapy was given after the procedure.Results The pressure gradient across the inferior vena cava was reduced from 16.8?4.3 mmHg preoperatively to 2.6?0.6 mmHg postoperatively(t=13.280,P=0.001).The IVCS symptom scores were decreased from 4.4?1.6 preoperatively to 2.1?1.7 postoperatively(t=6.880,P=0.010).Symptoms of edema on lower limbs,scrotal or pubic dropsy,ascites,and anasarca subsided at 1～4 days after procedure.Conclusions Endovascular treatment of inferior vena cava syndrome is effective and reliable.

Objective To investigate the mutation of endothelin-B receptor(EDNRB) gene in sporadic Hirschsprung′s disease in Chinese population, to study the relationship between EDNRB gene and the pathogenesis of HD. Methods Genomic DNA was extracted from HD bowel tissues removed by surgery in 34 sporadic HD patients. Exon 3, 4, 6 of EDNRB gene of EDN-3 gene were amplified by polymerase chain reaction (PCR) and analyzed by single strand conformation polymorphism (SSCP). Results EDNRB mutations were detected in 2 of the 13 short-segment HDs,one′s mutant was in the exon 3, the another was in the exon 6. No mutations were detected in the ordinary or long-segment HD. Conclusions The mutations of EDNRB gene were detected in the short-segment HD of sporadic HD in Chinese population. The results suggest that the EDNRB gene plays an important role in the pathogenesis of HD.

AIM: To determine adenosine content in Kudiezi Injection(Ixeris sonchifolia Hance). METHODS: HPLC was used with Shim-pack CLC-ODS(6.0 mm?150 mm,5 ?m). The mobile phase consisted of methanol-acelonitrile-0.025 mol?L -1 phosphoric acid (3∶4∶93). The flow rate was 1 mL?min -1 . The UV detection wavelength was at 260 nm. RESULTS: The adenosin content in Kudiezi Injection was 0.15/10 mL. The average recovery was 98.4%(n=5). CONCLUSION: HPLC can be used for the quality control of Kudiezi Injection Injection.

AIM: To investigate whether Ligustrazine(LTZ) has an effect on the changes of protein kinase C(PKC) signaling pathway induced by inflammatory mediators involved in asthma in normal human peripheral blood lymphocytes (PBL). METHODS: 10 mL peripheral venous blood was obtained from each of 63 health humans and treated as follows. The activities of PKC from cytosolic and membrane fractions in PBL were measured by -ATP-catalyzing assay, after PBL had been isolated and performed by following processes: (1) First: three groups treated with 5 g/L LTZ(n=6) or 5 ?mol/L Ro31-8220 (n=6); Paired untreated PBL served as control of this group, as well as the negative controls of the following groups(n=6); (2)Second : three groups treated with 100 nmol/L Methacholine (Mch, n=5), 5 g/L LTZ+100 nmol/L Mch(n=5)or 5 ?mol/L Ro31-8220(a PKC inhibitor)+100 nmol/L Mch(n=5); (3)Third: three groups treated with 100 nmol/L histamine, 5 g/L LTZ+100 nmol/L histamine(n=5) or 5 ?mol/L Ro31-8220+100 nmol/L histamine(n=5); (4)Fourth: three groups treated respectively with 100 nmol/L PMA(a PKC activator, n=5), 5 g/L LTZ+100 nmol/L PMA(n=5) or 5 ?mol/L Ro31-8220+100 nmol/L PMA(n=5). RESULTS: (1)LTZ had no effect on the activities of PKC in inactive PBL in normal humans; (2) Methacholine or histamine resulted in an increase in membrane PKC activity of normal human PBL, which was partly suppressed by LTZ (all P

AIM:To investigate the activity change of voltage-dependent delayed rectifier potassium channel(Kv) on human airway smooth muscle cells(HASMCs) after transfection of Kv1.5 antisense oligonucleotides(AsOND),and to discuss the regulating mechanism of Kv1.5.METHODS: The mRNA and protein expressions of Kv1.5 in liposome-mediated Kv1.5 AsOND transfected HASMCs were measured with techniques of reverse transcriptase-polymerase chain reaction(RT-PCR) and Western blotting.Kv activities in transfected HASMCs were investigated with whole-cell patch clamp.RESULTS: After HASMC were transfected by liposome-mediated Kv1.5 AsOND,the mRNA and protein expressions of Kv1.5 were decreased,and Kv activity was inhibited,which made the cell membrane potential(Em) inclined to depolarization.CONCLUSION: Transfection of Kv1.5 AsOND made the function of Kv in HASMCs decreased.Kv1.5 may play a critical role in the regulation of Kv activity.

OBJECTIVETo investigate changes in the delayed rectifier K+ channel (Kv) function and the regulation of Kv by the protein kinase C (PKC) pathway in bronchial myocytes from asthmatic rats.

METHODSThe Kv currents and membrane potentials in bronchial myocytes from asthmatic rats and from controls were observed, using whole cell voltage- and current-patch clamp techniques.

RESULTSBronchial myocytes from asthmatic rats showed a significant reduction in Kv-current density (51.6 +/- 9.4 pA/pF, n = 14, P < 0.01) in comparison with those from control rats (72.4 +/- 12.3 pA/pF, n = 14) at + 50 mV. The current-voltage relationship curve exhibited a significant downward shift. Bronchial myocytes from asthmatic rats had no significantly different capacitances (P > 0.05), but had more positive membrane potential (P < 0.01) compared with those from controls. 1 micro mol/L phorbol 12-myristate 13-acetate, a PKC activator, caused an obvious reduction in Kv-current density (P < 0.01) and a significant downward shift in the current-voltage relationship curve, an effect which was partly abolished by 1 micro mol/L Ro31-8220 (a PKC inhibitor); 1 micro mol/L phorbol 12-myristate 13-acetate caused more positive membrane potential (Em), from -36.8 +/- 5.7 mV to -30.4 +/- 7.3 mV, in rat bronchial myocytes (P < 0.05). This effect was partly abolished by 1 micro mol/L Ro31-8220.

CONCLUSIONSBronchial myocytes from asthmatic rats have inhibited Kv function, more positive membrane potential, and higher excitability, all of which can also be induced by PKC activation. These characteristics may contribute to the development of airway hyperreactivity in asthma.

Animals ; Asthma ; physiopathology ; Bronchi ; cytology ; Membrane Potentials ; Myocytes, Smooth Muscle ; physiology ; Potassium Channels ; physiology ; Protein Kinase C ; metabolism ; Rats ; Rats, Sprague-Dawley