1.Protecting Effects of Different Time-course Electroacupuncture Pretreatments on Blood-brain Barrier Function in Cerebral Ischemia Rats
Shanghai Journal of Acupuncture and Moxibustion 2014;(12):1169-1172
ObjectiveTo investigate the impactsof different time-course electroacupuncture pretreatments on brain water content and blood-brain barrier permeability and assess their protecting effects on blood-brain barrier functionin cerebral ischemia rats. MethodClean-grade healthy male SD rats were randomly allocated to five groups (n=48): model control group, and one-day, three-day, seven-day and fifteen-day electroacupuncture pretreatment groups. A rat model of unilateral middle cerebral artery occlusion (MCAO) was made after electroacupuncture pretreatment was finished in various electroacupuncture groups. The water content of the brain and the amount of EB through blood brain barrier were measured at 12, 24 and 48 hrs after cerebral ischemia. ResultBoth brain water content and EB permeation amount were lower at 12 and 48 hrs than at 24 hrs after cerebral ischemia (P<0.05). There was a significant reducing effect on brain water content and EB permeation amount in the fifteen-day electroacupuncture pretreatment group at various time points after cerebral ischemia (P<0.05,P<0.01,P<0.001).Conclusion The blood-brain barrier is impaired most severely at 24 hrs of the three time points 12, 24 and 48 hrs after cerebral ischemia. Electroacupuncture pretreatment can produce a protecting effect on blood-brain barrier function in cerebral ischemia rats by markedly reducing cerebral edema and blood-brain barrier permeability.
2.Effects of gestational malnutrition on glucose and lipid metabolism of pregnant rats and birth weight of their offsprings
Chinese Journal of Perinatal Medicine 2013;(6):362-368
Objective To explore the glucose and liplid metabolism changes of pregnant rats and the birth weight of their offsprings due to pregnancy malnutrition,including overnutrition and undernutrition.Methods Thirty pregnant Wistar rats were randomly divided into three groups on the day of conception,the high-fat-diet group,normal control group and 50% food-restricted diet group.Increase of body weight and serum level of triglycerides,high-density lipoprotein,low-density lipoprotein,oral glucose tolerance test (OGTT) and insulin release test(IRT) levels during pregnancy were measured.The birth weight of offsprings was recorded within 12 h after birth.One-way ANOVA,LSD or Dunnett T3,and Chi-square test were applied for statistical analysis.Results The body weight at late pregnancy and the level of triglycerides and low-density lipoprotein were significantly increased in the high-fat-diet rats compared to normal control ones [(1.68±0.13) mmol/L vs (0.78±0.08) mmol/L,(0.57±0.04) mmol/L vs (0.35±0.07) mmol/L; both P<0.01].While the level of high-density lipoprotein of high-fat-diet rats was lower than that of the normal control rats [(0.56±0.06) mmol/L vs (1.09±0.08) mmol/L,P<0.05].The level of triglycerides,low density lipoprotein and high density lipoprotein in 50% food restricted group [(0.47±0.06) mmol/L,(0.21±0.06) mmol/L and (0.42±0.05) mmol/L] were lower than the control group (all P<0.05).Impaired glucose tolerance,abnormal OGTT and IRT results were found in both high-fat-diet and 50% foodrestricted diet rats.The fasting plasma glucose level in high-fat-diet group was significantly increased in late pregnancy compared to the control [(6.63±0.53) mmol/L vs (4.90±0.26) mmol/L,P<0.05],while there was no significant difference between rats in 50% food-restricted diet [(4.18±0.26) mmol/L]and control group (P>0.05).There was no statistical difference in fasting serum insulin level among the three groups.The average birth weight was significantly increased in high-fat-diet rats than in the control group [(6.14±0.31) g vs (5.73±0.26) g,P<0.05],and the incidence of macrosomic newborn rats in the high-fat-diet group was also higher than that of the control group [19.2 % (19/99)vs 7.84%(8/102),P<0.05].The birth weight of offspring in 50% food-restricted diet group was decreased with their average birth weight of (4.54±0.23) g,which was lower than that of the control group (P<0.05).The incidence of macrosomic [13.40% (11/76)] and low birth weight newborn rats [15.90% (13/76) vs 3.92%(4/102)] in food-restricted diet group were both higher than in the control group (P<0.05); and smaller number of offsprings was obtained than the control group (76 vs 102,P<0.05),the number of offsprings in high-fat-diet group was 99,which was less than the control group without statistical difference (P>0.05).Conclusions Pregnancy malnutrition,including overnutrition and undernutrition,may affect the lipid metabolism and glucose tolerance of pregnant rats,leading to insulin resistance,and may also contribute to the abnormal birth weight of the offspring,including macrosomia and low birth weight.
3.The Diagnostic Value of Clustered Calcification Found by Mammography in Breast Cancer
Huanghui LIU ; Wei WANG ; Xianming LUO
Journal of Chinese Physician 2001;0(09):-
30 and calcification distribution were the most reliable signs for the diagnosis of breast cancer. The shape and size of calcification also were helpful to the differential diagnosis of benign and malignant breast lesions.
4.Occlusion of Atrial Septal Defect via Small Chest Incision:Report of 41 Cases
Xianming ZHU ; Zhiping LIU ; Long ZHAO
Chinese Journal of Minimally Invasive Surgery 2001;0(02):-
Objective To discuss the surgical outcomes of atrial septal defects(ASD) by closing the ostium secundum via small chest incision.MethodsTotally 41 cases with ASD were treated by occlusion via a small incision(3-4 cm) at the right anterior chest.After cutting the pericardium,the right atrial was sutured with double ring and a special occluding device was inserted to close the ostium.Afterwards,under the guidance of transthoracical or transesophageal echocardiography,the satisfiable position for the occluder was confirmed by repeated testing with the protect string.After that,the right atrial and the occluder were sutured by a needle thread for fixation.ResultsOf the patients,the procedure was successfully completed in 39 cases.The mean operation time was 45 to 95 minutes(mean,60 minutes).The patients were discharged from our hospital in 3 to 6 days.No dislocation of the device or atrial shut was found in 2 to 24 months after the operation.Two patients were converted to open surgery because of failure in the occlusion.ConclusionOcclusion via small chest incision is safe,minimally invasive,and convenient procedure for ASD.
5.Effects of Dexamethason on the Expressions of STAT1 and PDGF in Lung Tissue of Pulmonary Fibrosis Rats Induced by Bleomycin.
Jinping LIU ; Nailiang ZHAI ; Xianming FAN
Journal of Medical Research 2006;0(02):-
Objective To study the effect of dexamethasone on pulmonary fibrosis model induced by bleomycin in rats and the expression of STAT1 and PDGF in lung tissue,and to discuss the possible mechanism.Methods Forty-five Wistar rats were randomly divided into three groups(15 in each group).They were dexamethasone group(DXM group),bleomycin group(BLM group) and control group(NS group):① The DEX group were intratracheally instilled with belomycin(BLM)(5mg/kg,in 1ml 0.9% NaCl solution),and then were treated with dexamethasone 3mg/kg via celiac injection daily;② BLM group were intratracheally instilled with BLM(5mg/kg),and then were treated with saline 3mg/kg via celiac injection daily;③ NS group were intratracheally instilled with saline(5mg/kg),and then were treated with saline 3mg/kg via celiac injection daily.Five rats in each group were sacrificed at 7,14,and 28 days after intratrachel instillation.Histological changes of the lungs were evaluated by HE stain.The bronchoalveolar lavage fluid(BALF) of right lung was gotten and the cells counting as well as differentiation were figured out.The expression of STAT1 and PDGF protein was assessed by immunohistochemistry.Results(1) The total number of inflammatory cells in BALF in BLM group was the highest at day 7 and diminished at day 14 and 28,which was significant different compared with that of the NS group(P
6.An Experimental Study on the Treatment of Blast Deafness by Sodium Salicylale
Jinling WANG ; Shunli LIU ; Xianming CHEN
Journal of Audiology and Speech Pathology 2001;9(1):1-4
Objective To explore the effects of treatment and mechanism of sodium salicylate on blast deafness. Methods Guinea pigs were divided into blast group, blast plus sodium Salicylate group and sodium solicylate group. The animals in the blast group were exposed to blast average peak pressure 176.7 dB(SPL); sodium salicylate was injected IP 400 mg/kg,1/d. Monitoring ABR threshold, the value of MDA of and counting the number of hair cells on the cochlea preparation, SEM for hair cells was observed. Results The ABR threshold shift in the blast group was 46.75±13.56 dB,38.0±12.4 dB,35.5±10.67 dB,41.5±12.57 dB,33.75±8.42 dB, post-blast 1 d,3 d,1 w,2 w,4 w respectively. And that of the blast plus sodium salicylate was 46.65±11.7 dB,38.25±9.52 dB,30.5±9.17 dB,30.0±9.17 dB,23.0±6.57 dB. The two groups differed significantly at 1 w,2 w,4 w(P<0.05). The value of MDA differed significantly at 2 w,4 w(P<0.05). And the hair cells lost also differed significantly. It was observed that sodium salicylated can raise the ABR thresbold temporarily 24 hrs during simple sodium salicylate adminastration. However it recovered 14 hrs after the injection.Conclusion Sodium salicylate can partially attenuate the hearing loss caused by blast. It is probable that sodium salicylate can clear peroxicative produced during blast and therefore protect hair cells and make hearing loss partially recover.
7.Expression of β-tubulin Ⅲ in colon adenocarcinoma tissue and its clinical significance
Junzhong SHI ; Jianbin ZHUANG ; Chuanshan ZHANG ; Xianming LIU ; Dalu KONG
Journal of Jilin University(Medicine Edition) 2017;43(4):770-775,前插4
Objective:To detect the expression and clinical significance of β-tubulin Ⅲ in cancer tissue of the patients with colon adenocarcinoma,and to explore its clinical significance.Methods:A total of 111 colon adenocarcinoma tissue samples were obtained.According to the location of β-tubulin Ⅲ positive cells, all patients were divided into front group (n=72) and non-front group (n=39).The positive expression rate of β-tubulin Ⅲ in the patients with colon adenocarcinoma was detected with immunohistochemistry.The correlations among the expression of β-tubulin Ⅲ and gender,age,tumor differentiation, clinical stage, lymph node metastasis, recurrence and death were analyzed.Results: The expression levels of β-tubulin Ⅲ had no significant differences between the patients with different gerder,age,lymph node metastasis,clinical stages,death and recurrence.The positive expression rates of β-tubulin in cancer tissue of the patients had significant difference between front and non-front groups (χ2=8.76, P=0.01).Lowly-to-moderately differentiated tissue was more common in front group, and highly-differentiated tissue was more common in non-front group(χ2=6.88, P=0.03).There were significant differences in the expression levels of β-tubulin Ⅲ between cancer tissues with different differentiation degrees (χ2=5.74, P=0.04).In non-front group, lymph node metastasis was closely correlated with the expression of β-tubulin Ⅲ (χ2=6.02,P=0.05).The results of immunohistochemical staining showed that the β-tubulin Ⅲ positive-expressing cells were colored brown-yellow.The number of cells with positive β-tubulin Ⅲ expression was significantly increased in highly differentiated tissue compared with low-differentiated tissue.Conclusion:The expression of β-tubulin Ⅲ is closely related to tumor differentiation in colon adenocarcinoma tissue.The highly differentiated colon adenocarcinoma tissue is more common in non-front group in which the expression of β-tubulin Ⅲ is related to lymph node metastasis.
8.Research on apoptosis changes of tensor veli palatini in patients with obstructive sleep apnea-hypopnea syndrome
Hongxun GONG ; Xianming CHEN ; Xian ZHANG ; Fengfang LIU
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2010;(3):108-111
Objective:To explore apoptosis changes of dilator muscles in the upper airway by detecting the expression of Bax, Bcl-2 in tensor veli palatini in patients with OSAHS.Method:The expression of Bax and Bcl-2 were detected in tensor veli palatini in 30 cases with OSAHS and 10 cases chronic tonsillitis without OSAHS by immunohistochemistry and image analytical system, and the results were analyzed.Result:①The expression levels of Bax in the OSAHS group increased significantly compared to control group(P<0.05), but there were no significant differences of Bcl-2 expression between two groups, the ratio of Bax/Bcl-2 increased significantly(P<0.05). ②There were positive correlations between AHI and the expression levels of Bax(r=0.697,P<0.01) respectively in the test group.Conclusion:The results indicate that apoptosis occurred in tensor veli palatini in patients with OSAHS, and the more severity of OSAHS , the more apoptosis.
9.Correlated factors of pulmonary metastasis of hepatocellular carcinoma
Yongyi ZENG ; Xiang ZHANG ; Jingfeng LIU ; Yang ZHONG ; Xianming WANG
Chinese Journal of Digestive Surgery 2013;12(9):668-671
Objective To investigate the correlated factors of pulmonary metastasis of hepatocellular carcinoma (HCC),so as to provide theoretical evidences for the prevention and treatment.Methods The clinical data of 862 patients with HCC who were admitted to the First Affiliated Hospital of Fujian Medical University from January 2008 to March 2012 were retrospectively analyzed.There were 107 patients with pulmonary metastasis.Factors including serum alpha fetoprotein (AFP) level,serum gamma-glutamyl transpeptidase (GGT) level,hepatitis B virus (HBV) infection,presence and treatment of intrahepatic tumor were analyzed to screen out relevant factors of pulmonary infection of HCC.Univariate and multivariate COX regression model analysis were performed for data analysis.Results The results of univariate analysis showed that high level of AFP (≥400 μg/L),ultra-high level of GGT (≥ 150 U/L),presence of HBV infection,the number of intrahepatic tumors ≥2,no radical resection (or radiofrequency ablation) for intrahepatic tumors,combining with tumor thrombi in the vessels,lymph node metastasis were risk factors of pulmonary metastasis of HCC (RR =1.986,3.653,0.365,3.675,0.252,0.379,0.352,P < 0.05).The results of multivariate analysis showed that high level of AFP (≥400 μg/L),HBV infection,the number of intrahepatic tumors ≥2,no radical resection (or radiofrequency ablation) for intrahepatic tumors,combining with tumor thrombi in the vessels were risk factors of pulmonary metastasis of HCC (RR =2.391,3.462,3.425,3.396,2.418,0.638,P < 0.05).Conclusions AFP ≥400 μg/L,HBV infection,the number of intrahepatic tumors ≥ 2,no radical resection (or radiofrequency ablation),tumor thrombi in the vessels and lymph node metastasis are risk factors of pulmonary metastasis of HCC.Anti-hepatitis virus treatment and early treatment are helpful for the prevention and treatment.
10.Clinical characteristics of patients with primary pulmonary lymphoma
Lanfang LI ; Huaqing WANG ; Qian FAN ; Huilai ZHANG ; Xianming LIU
Chinese Journal of General Practitioners 2011;10(8):577-578
Clinical data of 13 patients with primary pulmonary lymphoma(PPL),treated in Tianjin Medical University Cancer Hospital from January 1999 to December 2009,were retrospectively reviewed.There were 8 patients with mucosa-associated lymphoid tissue(MALT)marginal zone lymphoma,2 patients with diffuse large B cell lymphoma,1 patient with NK/T cell lymphoma,1 patient with nodular sclerosis Hodgkin's lymphoma,and 1 patients with lymphocytic predominance Hodgkin's lymphoma.The main clinical symptoms were cough,fever,night sweat and weight loss.Two patients did not have any symptoms.Pulmonary consolidation shadows and multiple nodules were the main findings of CT scan.An air bronchogram was often seen in the consolidation imaging.The overall 5-year survival rate was 9/13 for all patients;while that was 7/8 for MALT marginal gone lymphoma.