1.Discussion on the Necessity and Demand of the Training for Professional Personality of Medical Student-One of the Research on Medical Professional Personality
Chinese Medical Ethics 1996;0(01):-
With the development of knowledge economy, society has more and more high demand for professional quality. Medical students are the reserve talents for the health care industry of our country, so it plays an important role in keeping people's health by improving the professional quality of medical students. Therefore, we should put more emphasis on the education of professional consciousness, professional moral, professional spirit, professional ideal and professional rights and interests, making students form the healthy medical professional personality, to establish the foundation for the sustainable development of the health care industry.
2.Screening for CHO cell line stably expressing inducible costimulator protein and its biological activity
Qingli DING ; Menglei LIU ; Xianlian LONG ; Qian SHEN
Academic Journal of Second Military Medical University 1981;0(03):-
Objective:To construct a recombinant retroviral vector carrying human inducible costimulator (ICOS) gene,screen for CHO cell line stably expressing ICOS protein and to study its biological activity.Methods: ICOS cDNA was obtained from human peripheral blood mononuclear cells (PBMC) through RT-PCR and was cloned into retroviral vector to construct retroviral recombinant pMSCV-ICOS; the latter was then packed and the high-titer virus producing cells were screened.Then CHO cell was infected by this high-titer virus and the stable cell line was screened.CHO-ICOS cells were co-cultured with PBMC (the ratio of CHO-ICOS to PBMC being 11,12,15, and 110) in presence of substimulating dose of anti-human CD3 antibody.The proliferation of PBMC and the CD25 expression on T cells were examined by 3H-TdR incorporation method and flow cytometry,respectively.CHO-pMSCV cells co-cultured with PBMC (11) served as the negative control and PBMC served as blank control.Results: We successfully constructed the retroviral recombinant pMSCV-ICOS and obtained CHO cell line stably expressing ICOS protein.3H-TdR incorporation method and flow cytometry showed that,compared with the negative control group and the blank control group,co-culture with CHO-ICOS cells significantly inhibited the anti-CD3 antibody-induced activation and proliferation of PBMC(P
3. Cellular and molecular genetic analysis of sex chromosome chimerism and dicentric isochromosome structural abnormalities: a report of two cases
Jian ZHANG ; Yunrong MA ; Xianlian LEI ; Shuang ZHANG ; Yuanyuan LIU ; Lulu GUO ; Shiyu ZHENG ; Jingjing PAN
Chinese Journal of Perinatal Medicine 2019;22(11):787-792
Objective:
To investigate the value of karyotype analysis, bacterial artificial chromosomes-on-beads (BoBs), chromosome microarray analysis (CMA) and fluorescence