Objective To evaluate the clinical validity of physiological and operative severity score for the enumeration of mortality and morbidity (POSSUM) in aged patients undergoing thoracic surgery. Methods Physiological and operative severity scores were kept in 206 aged patients undergoing thoracic surgery, in which peritoneal soiling was converted to pleura soiling. The physiological score and the operative risk score were compared between the groups with or without the postoperative complications. The mortality and morbidity rates were calculated. Actual mortality and morbidity rates were compared with the rates predicted by POSSUM. Results Among 206 patients, there were postoperative complications in 97 patients. The physiological score and the operative severity score were significantly higher in the group in whom complications occurred compared with those without complications (19.3?0.7 vs 15.1?0.6 for physiological score, P

Aim To investigate the effect of effective fraction of Xie-Xin decoction and their compatibilities(EFX)on NF-?B and I?B expression of lung injury rats induced by LPS.Methods Rats were pretreated for 4 days with total flavones(TFL),total free anthraquinones(TFA),total conjugated anthraquinones(TCA),the high(low)dose of compatibility of TFL and TCA(A high,A low),the compatibility of TFL and TCA(B)and dexamethasone respectively 4 days,then injected intravenously with LPS to induce lung injury.6 rats were sacrificed respectively in every group at 1,2,4 h after LPS administration,and the right middle of the lung was harvested at every time point,the expression of NF-?B and I?B was evaluated by Western blot.Results TFA、A high and Dex can inhibit the nuclear translocation of NF-?B at 1,2,4 h(P

AIM: To observe the influence of Toufengyu Dropping Pill(Rhizome chuanxiong,Radix angelicae dahuricae,etc) on neuropeptide and vasoactive substances in rat's migraine model. METHODS: Rat's migraine model caused by nitroglycerin were given Toufengyu Dropping Pill and rat's behavior and blood levels of NO,CGRP,ET,TXB_2 and 6-keto-PGF_(1a) were measured. RESULTS: The experimental results showed that Toufengyou Dropping Dill could obviously decrease rat's positive reaction number,the blood levels of NO,CGRP,TXB_2 and 6-keto-PGF_(1a),and increase the blood plasma level of ET,and rectify the imbalance of ET/NO ratio. CONCLUSION: Toufengyou Dropping Pill can affect the contents of neurotransmitter,neuropeptide in rat's migraine,then decrease the algoenic and inflammatory substances,relieve the neurogenic inflammation,and increase the pain threshold value,finally be quite effective for migraine.

Objective To evaluate evidence-based practice of catheter removal strategy in patients with short-term indwelling catheter after partial nephrectomy. Try to apply the best evidence into practice, and further to improvethe quality of clinical nursing through quality review. Methods Two rounds of quality review were carried out in the 2 wards from July to December in 2016. Using the 5 review criteria of best evidence, we reviewed baseline of catheter removal strategies for patients with short-term indwelling catheter after partial nephrectomy and developed appropriate evidence-based practice. Further, the best evidence was integrated into clinical nursing practice, and the prognosis was reviewed after then. Results In the baseline review, the compliance of the 5 review criteria was 0. However, after introducing the best evidence into nursing practice,the compliance was up to 100%. The mastering of evaluation knowledge of catheter removal of nurses increased from 73.00 ± 9.35 to 94.81 ± 3.38 with significant difference (t=12.72, P<0.01). The indwelling time of catheter shorted from (5.69±0.79) d to(4.24±0.82) d. The difference was significant (t=5.47, P<0.01). Conclusions Evidence-based nursing practice improvesthe strategies of short-term catheter removal after partialnephrectomy, while closer cooperation between nurses and doctors are still needed to promote the continuous improvement of nursing quality.

Objective To investigate the preventive and therapeutic effects of Tiaozhi Baogan Capsule (TBC) on fatty liver in rats and to explore its therapeutic mechanism. Methods SD rat models of fatty liver were set up by feeding high fat diet. After the treatment of TBC, the general physical state, liver index (the ratio of liver weight with body weight) , hepatic pathological changes , blood biochemical indices were observed in model rats. Results TBC could obviously ameliorate liver pathological changes in model rats, relieve hepatic steatosis and hepatocellular necrosis, decrease the content of malondialdehyde( MDA) and increase the vitality of superoxide dismustase (SOD) in serum and liver, lower the content of total cholesterol (TC) and low- density lipoprotein (LDL) in serum and decreae the liver index (P

AIM: To study the effect and mechanism of Baogan Xiaoshi Capsule(BXC) (Rhizoma Coptidis,Fructus Evodiae,Rhizoma Alpiniae Officinarum,Rhizoma Cyperi ,Rhizowa Atractylodis Macro cephalae,Fructus Aurantii Immaturus,Fel Bovis) preventing lithogensis of pigment gallstones in guinea pigs. METHODS: Feeding guinea pigs on lithogenous diet,BXC was taken for 3 months at the same time.In the end,the gallstone formation rate,biochemical indicators and liver morphology were observed. RESULTS: The gallstone formation rate was 76.92% in model group,and in BXC group,at middle or upper dose,it was 25%,20% respectively.BXC could increase the concentration of conjugated dilirubin and glycocholic acid,and decrease that of unconjugated dilirubin,Ca~(2+) and H~+.It could weaken the activity of endogenous ?-Glucuronidase and Ca~(2+)Mg~(2+)-ATPase,and also could strengthen that of Na~(+)K~(+)-ATPase.It could improve the fatty degeneration of liver. CONCLUSION: The results show that BXC could prevent pigment gallstone formation and the fatty degeneration of liver.The mechanism may be that BXC can weaken the activity of ?-Glucuronidase and decrease [Ca~(2+)].

The liquid-chromatography-mass technology was used in the metabolomic analysis of mouse's blood 1 hour after exhaustive exercise,in order to explore the potential mechanism of Rhodiola in fatigue elimination of exhaustive exercise mouse.The exhaustive mouse model was made by loaded-swimming.A total of 24 mice were randomly divided into theRhodiola + exercise group,exercise group andno-exercise group.The dose of Rhodiola was 0.4375 g·kg-1·d-1.The loaded-swimming was conducted after two successive weeks of medication.Blood was collected 1 h after swimming for the sample preparation.The enzyme assay and anthrone colorimetry were used to test blood lactate acid and glucose,respectively.UFLC-Q-TOF was used to detect metabolic profiles of each group.The principal component analysis (PCA),orthogonal partial least squares discriminant analysis (OPLS-DA) and heat map analysis were used to compare differences among groups with score chart and to obtain the characteristics biomarkers by load chart.The results showed that the blood lactate acid level of theRhodiola+ exercise group was significantly lower than that of theexercise group.And the glucose level of theRhodiola+ exercise group was significantly higher than that of theexercise group.The metabolomic analysis showed that there were no obvious changes on 1,25-(OH)2D3,diacylglycerol (DG) and inositol triphosphate (IP3).All three materials in theRhodiola + exercise group were significantly lower than those of theexercise group.They were much closer to theno-exercise group.And all three materials were related to the increasing of muscle tension.It was concluded that Rhodiola had the function of promoting fatigue eliminating.This effect may be related to cell membrane protection,regulation of 1,25-(OH)2-D3→IP3,DG pathway,and relieving of muscle tension after exercises.

Objective To investigate the role of deoxycholic acid (DCA) in the pathogenesis of Barrett esophagus.Methods Normal human esophageal mucosal epithelial cells were cultured in vitro with defined keratinocyte serum-free media (D-KSFM).The cultured cells were treated with different concentrations of DCA and specific p38 mitogen activated protein kinase (MAPK) inhibitor. The expression of p38,phosphorylated p38 (p-p38) and caudal-related homeodomain transcription 2 (CDX2) at protein level were assessed by Western blot.The correlation between p-p38 and CDX2 was analyzed.The data were analyzed by one way ANOVA and LSD test.Results After being cultured with DCA for 24 h,the expression of p-p38 and CDX2 increased along with the increasing of DCA concentration.Compared with the control group (p-p38 was 13.7％ ± 1.0％ and CDX2 protein was 0),the difference was statistically significant (P＜ 0.05).When DCA was at 500 μmol/L,the expression of p-p38 and CDX2 reached the highest level (44.0％ ± 1.7％ and 8.59± 1.25).After pretreated with SB203580 for two hours and then 500 μmol/L DCA was added into cell culture,both expression level of p-p38 and CDX2 decreased compared with 500μmol/L DCA group (p-p38:28.3％ ±2.2％ vs50.5％±9.5％,CDX2:0.94±0.13 vs 2.31±0.41) after 24 h.Conclusions DCA can induce the expression of CDX2 in normal human esophageal mucosal epithelial cells,which is related with the activation of p38.The phosphorylation of p38 maybe involved in the pathogenesis of Barrett esophagus.

OBJECTIVE:To study the litholytic,bacteriostatic and spasmolytic effects of Baoganxiaoshi capsule(BXC) in vitro.METHODS:Observed the effects of BXC on human bile pigmental and cholesterol gallstone, bacteria, duodenum smooth muscle of rabbits in vitro.RESULTS:The litholytic concentration of 7.568,15.135 and 30.270g(crude drug)/ml solutions of BXC on human bile pigmental gallstone were 5.4mg,8.3 mg and 14.7 mg respectively,and on human bile cholesterol gallstone were 2.9mg,5.1 mg and 7.1 mg respectively.So BXC have the litholytic function of bile pigmental and cholesterol gallstone.It can strongly inhibit or sterilize staphylococcus aureus, enterococcus, diphtherial bacillus, hemo1ytic streptococcus, and the MICs were 23.65mg(crude drug)/ml ,23.65mg(crude drug)/ml,23.65mg(crude drug)/ml and 47.297mg(crude drug)/ml respectively, and all of the MBCs were 47.297mg(crude drug)/ml.It can inhibit or sterilize coliform,salmonella and pneumococcus, and all of the MICs were 378.375mg(crude drug)/ml, and all of the MBCs were 756.75mg(crude drug)/ml.5.3 414mg(crude drug)/ml solutions of BXC could obviously inhibit the average tension amplified,the frequency accelerated,and the activity increaced,on the duodenum smooth muscle induced by ach(P