Objective To evaluate the safety and immunogenicity of SARS-CoV-2 inactivated vaccine(Vero cells)for people aged 60 and above with hypertension and/or diabetes. Methods In Songtao Miao Autonomous County,Guizhou Province,hypertensive and diabetic patients aged 60 and above and healthy people were selected as the research objects. They were divided into hypertension group,diabetes group,combined disease group(suffering from hypertension and diabetes simultaneously)and healthy people control group,and injected intramuscularly with SARS-CoV-2 inactivated vaccine(Vero cells)through the lateral deltoid muscle of upper arm at 0 and 21 d respectively,with a dose of 0. 5 mL each time. Adverse events in ≤ 30 min,0 ～ 7 d and 8 ～ 21 d after immunization were recorded and followed up to 6 months after vaccination. Venous blood samples of 5. 0 mL were collected before vaccination and 28 d after vaccination. The sera were separated,detected for neutralizing antibody levels by cytopathic assay and calculated for the geometric mean titers(GMTs)of neutralizing antibody and antibody seroconversion rate. Blood pressure of patients was measured in fixed time before vaccination and 30 min and 1 ～ 7 d after vaccination in hypertension group and combined disease group respectively;The fasting blood glucose was measured before vaccination and 2 h postprandial blood glucose was measured once on the day of vaccination. The fasting blood glucose and 2 h postprandial blood glucose were measured on 1,3,5 and 7 d after vaccination respectively. Results There was no significant difference in the incidence of solicited adverse events among hypertension group,diabetes group,combined disease group and healthy control group（χ2= 1. 790,P = 0. 617）;There was no significant difference in the incidence of non-solicitation adverse events(P = 0. 412). A total of 5 serious adverse events occurred,all of which were judged to be unrelated to vaccines. No abnormal fluctuation of blood pressure and blood glucose was observed. The lower limits of 95% CI of the rate difference of hypertension,diabetes and combined disease groups were all greater than-10%,and the lower limits of 95% CI of the neutralizing antibody GMT ratio were greater than 0. 67,all of which were not inferior to the healthy control group. Conclusion SARS-CoV-2 inactivated vaccine(Vero cells)has good safety and immunogenicity for people aged 60 and above with hypertension and/or diabetes.

Objective To evaluate the effect of motivational interviewing (MI) on the medication adherence and self-efficacy in patients with ulcerative colitis.Methods Totally 81 patients with ulcerative colitis were randomly divided into the control group(n=40) and the experimental group(n=41).The patients in the control group received routine ulcerative colitis education,while the patients in the experimental group received developed motivational interviewing intervention.The problems existing in treatment course were found out through interview and protocols were made with patients together.Results One and three months after the intervention,the scores of medication adherence in the experimental group were (7.20±0.85) and (6.57±1.02),higher than those of the control group(6.48±0.71) and (5.98±0.85),t=3.868,2.655,both P＜ 0.05.Self-efficacy scored higher in the experimental group than that of the control group,(229.73±14.61) vs.(213.60±13.06),t=5.233,P ＜ 0.01.Conclusions Motivational interviewing is much more effective than routine education on the improvement of medication adherence and self-efficacy in patients with ulcerative colitis.

Objective:To study the effect of processing on pharmacological actions Dipsacus asperoides and its different processed products.Methods:The analgestic effect of Dipsacus asperoides in mice was observed in hot plate and acetic acid-induced writhing test. The anti-inflammatory action was studied in mouse ear swelling test and capillary permeability test and anti-coagulation action in mice was also observed by mouse blood coagulation time test.Results:The experimental results show that all processed Products of Dipsacus asperoides can inhibit the pain and has the actions of auti-inflammation and auti-coagulation. Conclusion: The actions of its processed products with wine is the most powerful.

Objective To evaluate the reliability and validity of Diabetes Diet-Related Quality-of-Life Scale (DDR-QOL) in Chinese version,in order to provide an efficient instrument for assessing the quality of life of diabetes in diet therapy.Methods Translating and edit the DDR-QOL scale initially,275 eligible subjects were invited to the study.DDR-QOL scale and 36 item Short Form Health Survey (SF-36) were used to collect data which were conducted for evaluating the internal consistency,reproducibility,construct validity,convergent and discriminant validity of DDR-QOL.Results The total Cronbach's α was 0.86.The factor analysis suggested good construct validity of DDR-QOL.Significant correlations were found between DDR-QOL and SF-36.The quality of life of diabetes who is on the job or not can be identified by DDR-QOL.Conclusions The Chinese version of DDR-QOL is a valid and reliable tool to assess the quality of life of diabetes who are in diet therapy.

Objective To describe the factors influencing medication compliance of patients with ulcerative colitis (UC) and provide a reference for health care workers developing effective response plans.Methods In-depth interview was conducted to 10 UC patients and the interview results were analyzed by phenomenology research method.Results There were five factors influence medication compliance of UC patients including drug affect,economic burden,living events,follow-up appointment and stigma.Conclusions The medication compliance of UC patients is mainly related to patients lacking of drugs knowledge,heavy burden on drugs cost and shortage of social support etc.The health education and medication supervision on UC patients should be strengthened and the social health insurance system on UC needed to be improved.

AIM: To investigate the effects of dehydroepiandrosterone (DHEA) on experimental osteoarthritis in rats. METHODS: Forty rats were randomly divided into four groups. Group A is normal control group. Osteoarthritic models of rats were established by intraarticular injections of papain into the right knee joints of groups B, C and D. Then the right knee joints of rats in groups C and D, respectively, received 150 ?l intraarticular injections of DHEA at a concentration of 50 ?mol?L~(-1) and 100 ?mol?L~(-1), and the right knee joints of rats in groups A and B both received 150 ?l physiological saline, twice weekly for five weeks. Six weeks later, all rats were sacrificed, and the articular cartilage was assessed by gross morphologic, histologic, biochemical and immunohistochemical methods. RESULTS: The cartilage damage in groups C and D was much less than that in group B through observation under a surgical microscope. The Mankin's score, nitric oxide (NO) in the douche of articular cavity, malondialdehyde (MDA) in synovium, the expression of matrix metalloproteinase-1 and 9 in articular cartilage in groups C and D decreased in comparison with group B, and the foregoing indexes in group D decreased significantly compared with group C. However, the activities of superoxide dismutase (SOD) in the douche of articular cavity and blood serum in groups C and D increased in comparison with group B, and the foregoing indexes in group D increased significantly compared with group C. CONCLUSIONS:DHEA shows a cartilage-protecting effect which is in a dosage-dependent manner. The mechanism probably is to inhibit the expression of matrix metalloproteinases and to decrease the release of (NO and enhance the antioxidation.

Objective To investigate longitudinal systolic function of transplanted heart using two dimensional speckle tracking echocardiography and tissue Doppler imaging.Methods 56 consecutive patients with heart transplant were recruited,according to myocardial biopsy and 1 year's follow up,they were divided into non-rejection group (group A) and rejection group (group B).36 healthy controls (group C) were also randomly recruited.Left ventricular ejection fraction (LVEF),pulmonary arterial systolic pressure,tricuspid annular plane systolic excursion (TAPSE),tricuspid annular plane systolic velocity (TAS') and global longitudinal strain (GLS) were calculated via Qlab 9.0 analysis software offline.Results Compared with group C,LVEF was reduced in group B (P ＜0.01).Differences of GLS,TAPSE,and TA-S' among groups were all statistically significant (P ＜0.01),group B＜group A＜ group C.LVEF,TAPSE and TA-S' were correlated with GLS (r =-0.64,r =-0.69,r =-0.71 ; all P ＜ 0.01).Conclusions Left and right ventricles were a functional unity,the systolic function of which was impaired in patients with heart transplant.

ObjectiveTo evaluate left ventricular (LV) function and dyssynchrony in patients with dilated cardiomyopathy (DCM) and complete left bundle branch block (CLBBB) by three-dimensional speckle tracking imaging(3D STI).Methods3D STI was performed and analyzed using TomTec 4-D LV analysis 3.0 software in 37 DCM patients with CLBBB and 25 healthy volunteers.The global 3D,longitudinal,circumferential,radial strains were measured.LV dyssynchrony was evaluated by the standard deviation of time to peak from 3D strain of 16 segments related to the heart cycle(3D-SDI).ResultsIn control group,uniformity in the average value of 3D strain was observed between apical,mid-ventricular and basal levels (P ＞ 0.05).Global 3D,longitudinal,radial and circumferential strains had excellent correlations with LV ejection fraction ( r =- 0.92,- 0.84,- 0.78 and 0.81,respectively,P ＜0.01).Compared with control group,global 3D,longitudinal,radial and circumferential strains were significantly lower in DCM patients ( P ＜0.01 for all).3D-SDI in DCM patients with CLBBB was significantly longer than that of volunteers ( P ＜0.01).3D-SDI increased with worsening LV systolic function regardless of QRS duration (P ＜0.05).ConclusionsWhen image quality is optimal,3D STI represents a promising novel technique for assessment of global LV function and dyssynchrony.

Objective To quantitatively evaluate left ventricular layer-specific strain using speckle tracking imaging,and their relationship with age and gender in nornal subj ects.Methods GE Vivid E9 equipment with EchoPAC BT1 2 workstation were used.One hundred and nineteen healthy subj ects were enrolled in this study.Results Left ventricular global longitudinal and circumferential strain was highest in the endocardium and lowest in the epicardium.Longitudinal and circumferential 3-layer strain was highest in the apex and lowest in the base.Age and gender had little influence on the left ventricular layer-specific strain.Conclusions Speckle tracking imaging can quantitatively assess left ventricular layer-specific strain, providing another useful modality for evaluating cardiac function.

Objective To investigate the causes of complications induced by peripherally inserted central catheters(PICC)in cancer patients and summarize pertinent nursing measures.Method The clinical data of 26 tumor patients with PICC were analyzed retrospectively .Result The complications occurred in 8 patients with a rate of 30.8%, among which unsuccessful catheteration occurred in 1 case,oozing of blood at the mouth of catheter in 1 case,phlebitis in 1 case,dislocation in 1 case,skin allergy-like reactions in 2 cases and catheter emersion in 1 case,catheter breaking in 1 case and dislocation in 2 cases.Conclusion The skilled operation,preventive maintenance of catheters and health education to the patients are the important measures for prevention and reduction of the complications.