Objective To explore the mechanism that PDEF inhibition the invasion and metastasis of prostate cancer cells by down-reg uLated HIF-1α. Methods PEDF and PBS as the experimental group and control group were add to the c uLture medium of human prostate cancer cell line (PC3), the wound healing and transwell experiment were carried out to observed the ability of invasion and metastasis. And HIF-1α expression was detect by RT-QPCR. ResuLts The res uLts of wound healing and transwell showed that PEDF inhibit the ability of invasion and metastasis of prostate cancer. PEDF down-reg uLated the expression of HIF-1α. Conclusions PEDF inhibit the invasion and metastasis of prostate cancer by down-reg uLated the expression of HIF-1α.It will be the new target of tumor intervention and for the treatment of prostate cancer and other malignant tumors to provide adequate scientific basis and efficient drug candidates.

【Objective】 To analyze the diagnostic value of prostate health index (PHI) plus soluble E-cadherin (sE-cadherin) for prostate cancer (PCa), so as to provide reference for the methods to enhance the detection rate of this disease. 【Methods】 A total of 200 patients with benign prostatic hyperplasia (BPH) admitted to our hospital during Jan.2018 and Jan.2023 were selected as the BPH group, and 200 PCa patients were enrolled as the PCa group.According to the different stages of the disease, the PCa patients were divided into stage Ⅰ-Ⅱ (n=92) and stage Ⅲ-Ⅳ (n=108) groups; according to the presence or absence of bone metastasis, they were divided into non-bone metastasis (n=146) and bone metastasis (n=54) groups; according to the Gleason score, they were divided into ≤8 (n=111) and>8 (n=89) groups.Serum sE-cadherin was detected with ELISA kit.Total prostate specific antigen (tPSA), free prostate specific antigen (fPSA) and isoform \[-2\] prostate-specific antigen (p2PSA) were detected with chemiluminescence analyzer and PHI was calculated according to the formula.The diagnostic value of sE-cadherin combined with PHI for PCa was analyzed using receiver operating characteristic (ROC) curve analysis. 【Results】 Compared with the BPH group, the PCa group had elevated levels of sE-cadherin, tPSA, p2PSA and PHI (P<0.05).Compared with patients with stage Ⅰ-Ⅱ PCa, stage Ⅲ-Ⅳ patients had increased levels of sE-cadherin, tPSA, p2PSA and PHI (P<0.05).PCa patients with bone metastasis had higher levels of sE-cadherin, tPSA, p2PSA and PHI than those without bone metastasis (P<0.05).Patients with Gleason score >8 had higher levels of sE-cadherin, tPSA, p2PSA and PHI than those with Gleason score ≤8 (P<0.05).The area under the ROC curve (AUC) of sE-cadherin, PHI alone, and combined diagnosis were 0.719, 0.761, and 0.792, respectively, the combined diagnosis had the highest AUC. 【Conclusion】 The levels of sE-cadherin and PHI are higher in PCa patients than in BPH patients, which increase with the increase of stage, bone metastasis and Gleason score.PHI plus sE-cadherin testing may help for the diagnose of PCa.

Ureteral stricture in renal allografts is one of the common postoperative complications in kidney transplant recipients. Due to short ureter in renal allografts, endovascular treatment should be adopted before reconstruction surgery to avoid irreversible injury. Alleviating renal allograft injury, easing obstruction or establishing drainage channel are the key measures to treat ureteral stricture. In endovascular treatment, balloon dilatation and internal incision yield high recurrence rate, and long-term indwelling of self-expanding metallic ureteral stents may be a better option. Compared with traditional stents, metallic stents may maintain urinary tract patency for a long time and mitigate the irritation of lower urinary tract symptoms,with different indications and efficacy. Although all metallic stents may be displaced and occluded, it still plays a positive role in the treatment of ureteral stricture in renal allografts. In this article, the application of self-expanding metallic ureteral stent in ureteral stricture of renal allografts was mainly illustrated, aiming to provide reference for optimizing the treatment of ureteral stricture in renal allografts.