Objective To evaluate the effect of hyperoxygenated solution on myocardial injury in the rats with acute carbon monoxide (CO) poisoning.Methods Thirty pathogen-free adult male SpragueDawley rats,weighing 250-300 g,were randomly divided into 5 groups (n=6 each) using a random number table:control group (C group),acute CO poisoning group (ACP group),and different doses of hyperoxygenated solution groups (HP1-3 groups).CO 120 ml/kg was injected intraperitoneally to establish the model of acute CO poisoning.Hyperoxygenated solution 10,15 and 20 ml/kg were infused via the caudal vein at 1 h after intraperitoneal injection of CO in HP1-3 groups,respectively.At 24 h after intraperitoneal injection of CO,blood samples were collected from the caudal vein for determination of plasma creatine kinase (CK),creatine kinase-MB (CK-MB),lactic dehydrogenase (LDH) and alpha-hydroxybutyrate acid dehydrogenase (α-HBDH) activities using the automatic biochemical analyzer.The rats were then sacrificed,and myocardial specimens were obtained for examination of the pathological changes with a light microscope.Results Compared with group C,the plasma LDH,α-HBDH,CK and CK-MB activities were significantly increased in ACP and HP1-3 groups (P＜0.01).Compared with group ACP,the plasma LDH,α-HBDH,CK and CK-MB activities were significantly decreased in HP1-3 groups (P＜0.05 or 0.01).Compared with group HP1,the plasma LDH,α-HBDH,CK and CK-MB activities were significantly decreased in HP2,3 groups (P＜0.05).The pathological changes of myocardium were significantly attenuated in HP1-3 groups as compared with group ACP.Conclusion Hyperoxygenated solution can attenuate myocardial injury in the rats with acute CO poisoning.

Objective To evaluate the value of neck blood vessel colored doppler ultrasound (NBVCDU) and magnetic resonance angiography (MRA) to extracranial carotid artery stenosis in patients with transient ischemic attack (TIA).Methods After implementing NBVCDU and MRA examinations at the same time,45 TIA patients with at least one examination showing arteriostenosis in extracranial section were chosen to carry out cerebral digital subtraction angiography( DSA ),then the stenosis rate was calculated by American symptomatic carotid endarterectomy trial (NASCET) method.Results Regarding DSA as the gold standard,for 45 TIA patients that having 180 arteriostenosis in extracranial section, sensitivity,specificity,accuracy of NBVCDU examination was 93.51% ,95.15% ,94.44%, Kappa = 0.735; sensitivity,specificity,accuracy of MRA was 92.21% ,94.17% ,93.33% , Kappa =0.681; sensitivity,specificity,accuracy of NBVCDU combined with MPA was 97.40% ,99.03% ,98.33%, Kappa = 0.872.Conclusions The sensitivity and accuracy of arteriostenosis in extracranial section by NBVCDU examination is higher than that by MRA, and it is suitable in the crowd primary examination.NBVCDU combined with MRA has shown good consistence with DSA for diagnosing arteriostenosis in extracranial section,but can't replace DSA comlpetely.

Objective To investigate the clinical immune status of patients with severe sepsis shock.Methods A total of 45 patients with sepsis in general ward and 45 severe sepsis shock patients treated in our hospital were selected for the study,and were as control group and observation group,respectively.The T lymphocyte,B lymphocyte and CD3+ CD4+,CD3+ CD8+,NK cells,immunoglobulin IgG,IgA,IgM levels,complement C3,C4 were detected in the two groups.The cellular immune function,humoral immune function and inflammatory reactions were compared between the two groups.Results The T lymphocyte count in the observation group and the control group were (60.0 ±16.2)％ and (77.2 ± 18.5)％,respectively,and The B lymphocyte count were (25.1 ± 10.2)％,and (14.3 ±6.9)％,respectively.The CD3+ CD8+ in the observation group was significantly lower,and CD4+/CD8+ was significantly higher than that in the control group (P ＜ 0.05).The percentage of NK cells and CD3 + CD8 + in the two groups showed no significant difference (P ＞ 0.05).The concentration of C4 and IgM in the observation group were significantly lower than that in the control group (P ＜ 0.05),and there was no significant difference between the two groups in C3,IgG and IgA (P ＞ 0.05).The WBC count,procalcitonin and C reactive protein in the observation group were significantly higher than that in the control group (P ＜ 0.05).Conclusion Severe septic shock patients have severe immune disorder.

Objective To analyze the levels of plasma procalcitonin (PCT)and C-reactive protein (CRP) in 65 patients with septic shock.Methods A total of 65 severe sepsis patients (septic shock group) were selected in our hospital in ICU.After treatment,12 patients died (death group),and 53 cases survived (survival group).At the same time,63 patients with non septic shock were selected (control group).The PCT and CRP levels of the septic shock group and control group were compared and analyzed before and at 3,6 and 12 d after treatment.Results Before treatment,PCT,CRP levels in septic shock group were significantly higher than that in the control group (P ＜ 0.05).At 3,6,12 d of treatment,septic shock group was higher than the control group,the death group was higher than the survival group (P ＜ 0.05).Conclusion The levels of PCT and CRP are important in diagnosis of septic shock progress.

Objective To investigate the clinical immune status of patients with severe sepsis shock.Methods A total of 45 patients with sepsis in general ward and 45 severe sepsis shock patients treated in our hospital were selected for the study,and were as control group and observation group,respectively.The T lymphocyte,B lymphocyte and CD3+ CD4+,CD3+ CD8+,NK cells,immunoglobulin IgG,IgA,IgM levels,complement C3,C4 were detected in the two groups.The cellular immune function,humoral immune function and inflammatory reactions were compared between the two groups.Results The T lymphocyte count in the observation group and the control group were (60.0 ±16.2)％ and (77.2 ± 18.5)％,respectively,and The B lymphocyte count were (25.1 ± 10.2)％,and (14.3 ±6.9)％,respectively.The CD3+ CD8+ in the observation group was significantly lower,and CD4+/CD8+ was significantly higher than that in the control group (P ＜ 0.05).The percentage of NK cells and CD3 + CD8 + in the two groups showed no significant difference (P ＞ 0.05).The concentration of C4 and IgM in the observation group were significantly lower than that in the control group (P ＜ 0.05),and there was no significant difference between the two groups in C3,IgG and IgA (P ＞ 0.05).The WBC count,procalcitonin and C reactive protein in the observation group were significantly higher than that in the control group (P ＜ 0.05).Conclusion Severe septic shock patients have severe immune disorder.

Objective To analyze the levels of plasma procalcitonin (PCT)and C-reactive protein (CRP) in 65 patients with septic shock.Methods A total of 65 severe sepsis patients (septic shock group) were selected in our hospital in ICU.After treatment,12 patients died (death group),and 53 cases survived (survival group).At the same time,63 patients with non septic shock were selected (control group).The PCT and CRP levels of the septic shock group and control group were compared and analyzed before and at 3,6 and 12 d after treatment.Results Before treatment,PCT,CRP levels in septic shock group were significantly higher than that in the control group (P ＜ 0.05).At 3,6,12 d of treatment,septic shock group was higher than the control group,the death group was higher than the survival group (P ＜ 0.05).Conclusion The levels of PCT and CRP are important in diagnosis of septic shock progress.

Objective A model for studying oral ulcers induced by betel nut-extract was constructed in rats.Changes in the structure and diversity of oral flora were observed to explore the involvement of oral flora and local inflammatory factors in the pathogenesis of oral ulcers induced by betel nut-extract and to provide theoretical support for the prevention and treatment of oral ulcers in the clinic.Methods Thirty SD rats were randomly divided into normal,model and intervention groups(Guilin watermelon cream,8 mg/d for 7 days),with 10 rats/group.The oral mucosa of rats was subcutaneously injected with 10 g/mL of betel nut-extract to generate an oral ulcer model.The histomorphological changes were observed,and ulcer area and ulcer scores were assessed.Local oral tissue tumor necrosis factor-α(TNF-α),interleukin(IL)-2 and IL-8 levels were determined.Oral mucosal tissues were sampled for HE staining and analyzed for the structural distribution of oral flora and the diversity of microbial communities using high-throughput sequencing method.Results Compared with rats in the normal group,those in the model group had an increased ulcer area,significantly increased ulcer scores(P＜0.01),and significantly increased levels of TNF-α,IL-2 and IL-8 in the oral mucosal tissues(P＜0.01).The amount Streptococcus(P＜0.05)and Veillonella(P＜0.001)in the oral saliva of the model group rats was significantly reduced.The model group rats showed oral mucosal epithelial cell hyperplasia or focal necrosis,mucosal lamina propria edema,and hemorrhage accompanied by mass neutrophil and monocyte infiltration.Compared with the model group rats,the intervention group rats had significantly reduced ulcerated area(P＜0.05,P＜0.01)and ulcer scores(P＜0.05).And oral mucosal tissue levels of TNF-α(P＜0.01),IL-2(P＜0.05)and IL-8(P＜0.05),as well as significantly increased Streptococcus(P＜0.001)and Veillonella(P＜0.01)and significantly reduced Staphylococcus(P＜0.01)in the oral saliva.The degree of lesions in the oral mucosal tissues was significantly improved in the intervention group.Conclusions Betel nut-extract can be used to successfully reproduce a rat model of oral ulcer,and it is speculated that the development of oral ulcers after exposure to betel nut-extract may be related to an imbalance in the oral flora and local tissue inflammatory mediators.