Objective To explore the effect of Yatongning tincture root canal implantation on interleukin 8 ( IL-8 ) , cluster of differentiation 4 (CD4) and heat shock protein 70(HSP70) in in gingival crevicular fluid of patients with periodontitis.Methods 64 patients with periodontitis from December 2013 to September 2015 in stomatology department of Yuyao Second People’s Hospital were selected and according to the different treatment methods divided into treatment group and control group, 33 cases in treatment group, 31 cases in control group.Patients received root canal implantation in two groups, the treatment group received Yatongning tincture root canal implantation on the basis of control group.The clinical curative effect, incidence of pain, IL-8, CD4 and HSP70 levels were compared between two groups.Results The clinical curative effect of treatment group was 96.97%(32 cases), which was higher than 80.65% (31 cases) of control group (χ2 =4.373, P<0.05).The pain incidence of treatment group was 30.30%(10 cases), which was higher than 58.06% (18 cases) of control group (χ2 =5.006,P<0.05).After treatment, the IL -8 levels were higher and CD4, HSP70 levels were lower in two groups than those pre-treatment(P<0.05), compared with control group, the IL-8 levels were higher and CD4, HSP70 levels were lower in treatment group ( P<0.05 ) .Conclusion Yatongning tincture root canal implantation could enhance the clinical efficacy , relieve tooth tissue damage and improve the post-operative pain in patients.

Objctive To evaluate therapeutic effect of periocline ointment as an auxiliary treatment of adult serious chronic periodontitis. Methods 56 patients were divided into the test group and the control group in register sequence. Following initial therapy, patients in the test group were treated with PERIOCLINE into the periodontal pocket once a week for 4 weeks, while patients in the control group were treated with iodine glycerine. At the baseline level, the indexes of PLI,SBI,PD,AL were recorded and compared between the two groups on the 1st month, 3rd month and 6th month. Results All the indices of both groups were improved, compared with the baseline ( <0.05) . The results of the test group were better than those of the control group, and the differences were statistically significant (<0.05) . Conclusion Periocline ointment as an auxiliary treatment of adult serious chronic periodontitis is superior to compounded iodine glycerine in the amelioration of all the indices.

Numerous peptides that bind to a given target have been selected by phage display technology. However, some peptides isolated to date do not bind with high affinity to tumor or organ sites, even peptides were selected in vivo. Therefore, the biodistribution of 99mTc-labeled filamentous phage peptide library via MAG3 (mercaptoacetyltriglycine) were investigated to gain a better understanding of phage circulation in vivo. The experimental results showed that the liver and spleen were the organs of the greatest accumulation, while heart, muscle, pancreas and brain retained less radioactivity. In opposite to other tissues and organs, the radioactivity in stomach, intestine and bone gradually went up with time. The clearance of 99mTc-labeled phage in blood was very fast from 5 min to 30 min and then slowed down. When phage in vivo circulated at enough long period of time, some phage particles could extravasate in some organs or tissues and internalized there. In conclusion, the circulation time of phage in vivo should be experimentally determined beforehand according to the targeted organs and the specific location of target peptides in order to panning a peptide with high specificity and affinity to that target.

Objective To investigate the association between expression of PHH3 and CyclinD1 and their significance in breast invasive ductal carcinoma.Methods Immunostoemical MaxVision staining was used to detect the expression of PHH3 and CyclinD1 in 89 samples of breast invasive ductal carcinoma and 30 samples of normal breast tissues.The correlation was then analyzed between the expression of PHH3 and CyclinD1 and the clinical path-ological parameters as well as prognosis.Results The positive rate of PHH3 in breast invasive ductal carcinoma tis-sues(68 /89,76.4%)was significantly higher than that in normal breast tissues(2 /30,6.7%)(χ2 =45.051,P <0.05).The expression level of PHH3 was significantly correlated with tumor histological degree(χ2 =10.540,P <0.05),anillary lymph node metastasis(χ2 =6.852,P <0.05)and TNMstage(χ2 =5.634,P <0.05),but it was not related to the age(χ2 =1.054,P >0.05 )and the tumor diameter(χ2 =0.014,P >0.05 ).The positive rate of CyclinD1 in breast invasive ductal carcinoma tissues(62 /89,69.7%)was significantly higher than that in normal breast tissues(3 /10,10.0%)(χ2 =32.223,P <0.05).The expression level of CyclinD1 was significantly correlated with tumor histological degree(χ2 =13.328,P <0.05),anillary lymph node metastasis(χ2 =5.041,P <0.05)and TNMstage(χ2 =4.419,P <0.05),but it was not related to the age(χ2 =0.500,P >0.05)and the tumor diameter (χ2 =1.836,P >0.05).The expression of PHH3 was directly correlated to the CyclinD1 (r =0.497,P <0.05). Kaplan-Meier analysis showed that the expression of PHH3 was negatively correlated with prognosis.Log-Rank test indicated that there was significant difference(χ2 =10.468,P <0.05),CyclinD1 was negatively correlated with prog-nosis.Log-Rank test indicated that there was significant difference (χ2 =7.906,P <0.05 ).Conclusion High expression of PHH3 and CyclinD1 may be correlated with evolution and metastasis of breast invasive ductal carcino-ma.Combined analysis of PHH3 and CyclinD1 may provide a theoretical basis for prognostic information and treatment of patients with breast cancer.

Objective:Comprehensive evaluation on the puerperal women's health status,and probing those influencing factors on their quality of life. Methods:513 puerperal women received the survey on health status including physical, psychological and social function dimensions by relevant items of Quality of Life designed by WHO and related factors were analyzed by stepwise regression.Results:The QOL of puerperal women were lower than general women aged between 18-49 years old in three dimensions(91.44?4.05/97.84?3.66,u=25.95,82.42?7.02/89.81?8.50,u=17.53,88.87?4.99/98.12?4.59,u=29.47,均P

This study was undertaken to investigate mother-to-infant transmission of human parvovirus B19 and the significance of prevalence of B19 virus in abnormal fetuses in Guandong. The polymerase chain reaction (PCR)was established to detect parvovirus B19 DNA in 700 sera from 350 maternal-infant pair groups. The prevalence of B19 virus DNA was 1.14％ (4/350)and 0.28％(1/350)in the sera of pregnant women and cord blood of their neonates respectively. Parvovirus B19 DNA sequences were also detected in abnormal fetuses and new-born by PCR. The positive results were obtained in 5 samples of fetal tissues from 17 abnormal fetuses and in 3 those of neonatal tissues from 7 cases of neonatal death. The amplified products of PCR were identified to be the target DNA with Hae Ⅲ digestion. By in situ hybridization ,parvovirus DNA could be detected mainly in the nuclei of immature hematopoetic cells within fetal brain or spleen whose PCR tests were positive. The study results suggest that human parvovirus B19 infection does exist in maternal-infant transmission in Guangdong and might lead to harm on fetuses,but the prevalence rate of B19 virus may be very low. The evaluation of B19 virus infection might depend on reliable assay to determine present infection or past infection.

Aim To investigate the effect of orientin on proliferation and differentiation of 3T3-L1 pre-adipocytes and on insulin resistance(IR) in 3T3-L1 adipocytes and the possible mechanisms.Methods MTT assay and oil red O staining were applied to investigate the proliferation and the differentiation of 3T3-L1 pre-adipocytes, respectively.The intracellular triglyceride(TG) contents were detected by enzymatic analysis.IR model was induced with dexamethasone.A fluorescent glucose analogue, 2-NBDG, was used to measure the rate of glucose uptake.Western blot was used to detect the protein level of GLUT4 and phosphorylation of AMPK and ACC.The GLUT4 translocation was measured by fluorescent-immunohistochemistry.Results Orientin decreased the formation of lipid droplets and intracellular TG contents(P<0.01) in a concentration-dependent manner(P<0.05), but it had no obvious effects on the cell vitality.Under the IR state, orientin significantly increased 3T3-L1 adipocytes glucose uptake(P<0.05).Meanwhile, orientin up-regulated the protein expression of p-AMPK, p-ACC, and enhanced GLUT4 translocation and its expression.Conclusion Orientin can effectively inhibit the differentiation of 3T3-L1 pre-adipocytes and increase insulin sensitivity due to the activation of AMPK/GLUT4 signal pathway.

Objective:To identify the cytotoxicity of four dental casting alloys on human gingival fibroblasts.Methods: MIT assay method was used to evaluate the cytotoxicity of four alloys on human periodontal ligament fibroblasts(HPDLFs) isolated from healthy periodontal ligaments of subjects on the 2nd d,4th d and 7th d of culture.Flow Cytometry(FCM) was used to evaluate the cytotoxicity of four alloys to human gingival fibroblasts.Results: It was found that there was no apparent cytotoxicity of all the four alloys.Conclusion:MTT and FCM methods show the same results.These four alloys have good biocompatibilities.

Glycyrrhizic acid, as the main active ingredient of Glycyrrhiza, has anti-inflammatory, antiviral, antitussive, expectorant, immunomodulatory and other pharmacological effects. In recent years, many new clinical uses of glycyrrhizic acid have been found, and glycyrrhizic acid has an effect on human and animal cytochrome P 450. This paper summarizes the clinical application of glycyrrhizic acid and its effect on cytochrome P 450, providing reference for clinical rational use of glycyrrhizic acid preparation.

Objective To analyze the effect of miR-497 high expression on the gene expression profile of colon cancer cell line HCT116. Methods MiR-497 high expressing colon cancer cell model HCT116-497 and negative control HCT116-CON were established by lentiviral transduction. The human (V2) gene expression microarray was used to identify genes that were differentially expressed between colon cancer cells overexpressing miR-497 and the controls. The candidates were subjected to the gene ontology (GO) and KEGG pathway enrichment analysis by Molecule Annotation System 3.0 (MAS3.0). The differential expression of representative genes relative to inflammation were confirmed by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). Results Of all the differently expressed genes, 582 genes were down-regulated by at least 3-folds, which were enriched in inflammation-related signaling pathways in colon cancer cells overexpressing miR-497. The decrease in 15 representative genes was validated by qPCR. Compared with those in HCT116-CON cells, expressions of 10 genes in HCT116-497 cells, including CACNB1, FOS, IL-29, RPS6KA2, TNFSF15, IL-11, INHBC, CSF1R, JAK3 and IL-2Rβ, were decreased significantly, and there were statistical differences (all P< 0.05) Conclusion MiR-497 inhibits the mRNA expression of inflammation-related genes in colon cancer cell line HCT116.