AIM: To elucidate the cellular source of adrenomedullin (AM) in myocardial tissue, the effects of 5 substances on secretion of AM from cultured rat fibroblasts and myocytes were examined in this study. METHODS: Myocardial fibroblast and myocyte isolation and culture; AM was measured by radioimmunoassay; AM receptors were assayed by radioactive analysis method. RESULTS:Tumor necrosis factor ? (TNF?), lipopolysaccharide (LPS) and basic fibroblast growth factor (bFGF) all could stimulate AM synthesis and secretion in myocardial Fb and myocyte , and the amount of AM secreted by Fb was greater than that by myocytes, whereas transforming growth factor-?(TGF?),and interferon-?(IFN?) suppressed in it in myocardial Fb and myocytes. Myocardial Fb has two kinds of AM binding sites, but myocyte has only one high affinity- binding site.CONCLUSIOIN:Myocardial fibroblasts could synthesize and secrete AM, and there are AM receptors in both myocardial fibroblasts and myocytes.

Objective:To evaluate the effect of a hand hygiene culture program on hand hygiene compliance in a three grade A hospital .Methods:Direct observation methods were used to assess the hand hygiene compliance and hand hygiene quality .Results:After the hand hygiene culture program , the hand hygiene compliance rate and the hand hygiene accuracy rate of doctors raised from 30 .2%and 66 .7%to 65 .3%and 85 .8%.Those of the nur-ses raised from 52.2%and 80.0%to 87.6%and 93.3%.Conclusion:The hand hygiene program increased the hand hygiene compliance and reducing thd risk of hospital infection occurred .

Lung transplantation has become the most effective treatment of end-stage lung diseases. Along with persistent optimization of lung transplantation technique and perioperative management, the short-term clinical efficacy after lung transplantation has been significantly improved, whereas the long-term clinical prognosis remains unoptimistic. Besides chronic lung allograft dysfunction, postoperative malignant tumors also threaten the long-term survival of the recipients. Common malignant tumors following lung transplantation include nonmelanoma skin cancer, posttransplant lymphoproliferative disease and lung cancer. After solid organ transplantation, a large majority of the recipients require lifelong immunosuppressive therapy. The intensity of immunosuppressive therapy for the lung transplant recipients is generally higher than other organ transplant recipients. Immunosuppression is the main factor which leads to the impairment of anti-tumor immune monitoring function and promotes the incidence and development of malignant tumors. In this article, the risk factors, prevention and treatment of the most common malignant tumors after lung transplantation were reviewed, aiming to provide reference for comprehensive diagnosis and treatment of malignant tumors following lung transplantation.