Glioma is the most common form of brain cancer. Despite recent advances in the treatment of solid tumors, there are few effective treatments for malignant gliomas due to its infiltrative nature. It has important significance to improve the treatment of glioma through in-depth understanding the intracerebral metabolic characteristics and pharmacokinetics of chemotherapeutics. Brain microdialysis (B-MD), an effective method to monitor central nervous system anticancer drug disposition, conditions of drugs through the blood-brain barrier, basic pathophysiologic metabolism, bioactive compounds and the changes of neurotransmitter in brain, provides the unique opportunity to allow the simultaneous determination of unbound concentrations of drugs in several tissues, and directly measure gliomas biochemistry continuously. B-MD has been able to monitor the change of brain drugs, metabolites and neurotransmitters, dynamic analysis of the drug concentration and pharmacological effect after administration, pharmacodynamic interaction between drugs, receptor mechanism of drug transport, as well as feedback information of internal environment. B-MD is expected to provide reference for clinical individual chemotherapy of glioma, but also provide powerful tools for the evaluation of new anticancer drugs in vivo. In this review, a comprehensive overview of B-MD for studies on glioma is elucidated with special emphasis on its application to neurochemistry and pharmacokinetic studies.

Objective: In order to kuow the germicidal efficacy of orthophthalaldhyde. Methods: Carrier quantitative germicidal test and clinical use study. Results: the showed that 23℃,the average killing ratc after exposure to the stock solution of orthophthalaldhyde disinfectant for 90 min was 100% of Bacillus Subtitis Var.niger on carriers and for 10 min contact time could destroy completely the antigenicity of serum HBsAg,and for 5 min was 100% of bacteria. Incontinuous use for 10 days ,immersion in for 14 days it was no corrosion of carben steel and stainless steel apparatus, and no irritative to skin and mucous membrane. Conclusion: Orthophthalaldhyde has good germicidal efficacy and belongs actually no corrosion and no irritative disinfectant.

Objective: To inspect dynamically the air-microorganism in important hospital departments. Methods: Plate sedimentation was used for examination. Results: The average rate of reaching standard is 93.01% for the important hospital departments from 2001 to 2004.The rates of reaching standard for region Ⅰ,Ⅱ and Ⅲof the hospital were 76.89%,90.14% and 97.03%,respectively. Conclusion: The content of air-microorganism is affected by personnel flow,airy condition,season and disinfection measure.

To prevent the spread of bird flu to human,we applied four effective means of infection control: concept introduction,light-accelerant method,tracking administration,and draft of coping measures,which displayed the characteristics of infection control in scientific and affectionate ways,and enhanced the level of public health service.

Objective:To check the cleansing effect of light catalyst compound atomization in the hospital environment. Methods: Bacterium quantities were compared in the rooms treated with light catalyst compound atomization or routine cleansing. Results: In the room treated with light catalyst compound atomization,the rate of air bacterium reduction was 90.83% at 1 h,53.61% at 24 h,and 55.82% in average from 1 week to 12 weeks.Bacterium on object surfaces were kept lower for 8 weeks in the light catalyst compound sprayed room than in the routinely cleansed room.Conclusion:The light catalyst compound atomization can chronically cleanse the hospital environment without pollution.

Objective To evaluate the specificity of Architect chemiluminescence immunoassay ( CLIA) for diagnosis of syphilis in the clinical screening test.Methods 65 774 syphilis specific antibody results in the Beijing Tongren Hospital were retrospectively analyzed.In this study, Architect CLIA for diagnosis of syphilis was used as a screening test for the pre-operative patients from August 2011 to July 2012.All the repeatedly reactive samples were tested by the tolulized red unheated serum test ( TRUST) and Treponema pallidum particle agglutination ( TPPA) assay.Samples with with discordant results were verified by western blot ( WB) Finally, all the results were statistically analyzed by software SPSS 17.0.Results Among 65 774 samples, 940 (1.43%) were found to be repeatedly reactive using the Architect CLIA.Of these sera, 330 ( 35.11%) were reactive and 610 ( 64.89%) were nonreactive by TRUST.Because a TRUST titer of 1:1was not always sufficient to confirm a TPPA reactive result, all the samples with CLIA reactive results were finally performed by TPPA assays.The results showed that 843 ( 89.68%) were positive and 97 (10.32%) were negative.After arbitrated by the WB, out of 97 TPPA negative sera, 18 (18.56%) were positive;7(7.22%) were indeterminate and 72(74.23%) were negative.Conclusions The specificity of Architect CLIA for diagnosis of syphilis was 99.89%.The results showed that confirmation by TPPA on sera with screening test for syphilis by Architect CLIA effectively decreased the false-positive results and could be suitable for a routine supplementary for syphilis.However, if necessary, the WB should be further utilized.

This article mainly discussed that infection control is an important part of hospital management and plays an important role in medical quality management by reviewing and summarizing the development of the department of hospital infection control in nanjing general hospital.It put forward the goal and plan of the subject build in the future.

To discuss hospital infection control for laminar flow operation room. Ten measures of hospital infection control have been formulated for laminar flow operation room based on the danger factors of the surgery incision infection and the principle of the air purify process and filtration setting. A set of management system and detailed rules have been founded for the use of laminar flow operation room, to strengthen the notion that the patients are the all. It has met the requirement for the use of air-clean technique and setting, and guaranteed air purity.

ObjectiveTo evaluate the suitability of Nordtest guideline in estimating measurement uncertainty of routine tests in clinical laboratory.MethodsData of clinical laboratory of Beijing Tongren Hospital were collected,which came from 176 days of Internal Quality Control ( IQC ) from July 2010 to December 2010 and 6 times of External Quality Assesment ( EQA ) of NCCL from 2009 to 2010.The combined and expanded uncertainties of 21meaurements (sodium, potassium, chlorine,calcium,phosphrous,glucose,urea nitrogen,creatinine,uric acid,total protein,albumin,total bilirubin,direct bilirubin, alanineaminotransferase, aspartateaminotransferase, alkalinephosphatase, lactate dehydrogenase,glutamyl transpeptidase,creatine kinase,triglyceride and total cholesterol) were evaluated according to Nordtest guideline.ResultsOf all the measurements,expanded uncertainty of direct bilirubin ( 17.69％ ) was the highest.For some enzymes such as ALT,AST,ALP and LDH,expanded uncertainties were all over 10％ markedly influenced by the calibrator uncertainty.Expanded uncertainty of triglyceride was 12.7％,also largely influenced by calibrator uncertainty,while that of total cholesterol was 6.96％.ConclusionsNordtest guideline is suitable to evaluate the measurement uncertainty of routine assays in clinical laboratory.However,calibrator uncertainty should be taken into account in the process of evaluation.

Astragalus produced in Gansu were chosen as the raw material to leachate. Studied the antibiotic effects of the leaching solution on the cariogenic bacteria and compared with the imported bacteriostatic product MI. Streptococcus mutans and Lactobacilli were cultured in the medium for 24 h. The PH and A600 values were measured. Statistical analysis was conducted by using SPSS 13.0. The leaching solution of astragalus has the same inhibitory effects on the growth and acid production of streptococcus mutans and lactobacilli as MI.