Objective To explore the distributed regularity of constitutional types of patients who suffered the gastroenteric carcinomas and lung cancer by epidemiological investigation of constitutional types,in order to analyze the characteristics of different kinds of tumor patients.Methods The standardized Constitution in Chinese Medicine Questionnaire(CCMQ) was answered by 355 patients to evaluate the scores of all kinds of constitutional types.The individual constitutional type was distinguished by the top score method,and the distribution rules of constitutional types in different kinds of tumor patients was analyzed.Results Among the tumor patients,the gentleness type accounted for 17.46 percent,eight pathological constitutions accounted for 82.54 percent.The most two types of pathological constitutions in gastroenteric carcinomas patients were Yang-deficiency and Qi-deficiency,which accounted for 23.98 percent and 23.39 percent respectively,while the majority of that in lung cancer patients were Qi-deficiency(42.93%) and Yang-deficiency(15.76%).Conclusion The patients suffering from different kinds of tumor have different constitution characteristics.It is a better idea to integrate the constitution analysis with syndrome differentiation treatment in clinical practice.

Objective To investigate the role of OX40 in the mechanisms of memory T cells in islet transplant tolerance.Methods The expression of OX40 on native, like memory and memory CD8+T cells was detected by RT-PCR. Splenic T cells from B6 mice were injected into Rag-/- mice via the tail vein, and the Rag-/- mice were divided into three groups (n=8 each): control group, given IgG; treatment group, given anti-OX40L; and OX40 knock-out group, given T cells from OX40 knock-out B6 mice spleen. All recipients were induced into diabetes mellitus model after adoptive transfer. Islet transplantation was performed on all Rag-/- mice as recipients. The mean survival time of islet was observed.Results The expression of OX40 in native T cells, like memory T cells and memory T cells was 2.87, 111.24 and 146.15 respectively. The expression of OX40 in like memory and memory T cells was higher than in native T cells (P<0.05). Comparison with control group , The mean survival time of the DBA/2 islet allografts in treatment group (130 days) and OX40 knock-out group (125 days) was significantly longer than in control group (21 days, P<0.05).Conclusion The OX40 expression is high in memory T cells. The mean survival time of the islet allografts can be prolonged by blocking OX40/OX40L pathway. OX40/OX40L pathway may be the key point of transplant tolerance.

Breast cancer belongs to high morbidity malignant tumor for women. Some known aspects to breast cancer were etiological factor, pathogenesis and prescription based upon literature review of Traditional Chinese Medicine (TCM) in ancient times, and the valuable references to modern clinical medicine (CM) was offered. Some animal studies and modern clinical trials indicated that curative effect of integration of Chinese and Western Medicine in experimental group was better than that of Western Medicine treatment alone in control group. It aims to offer open-minded idea under syndrome differentiation for professional staff in clinic by arranging, inducing-and summarizing related records on breast cancer.

Objective To observe the influence of hIL-10-MSCs on apoptosis of rats subject to discordant liver xenotransplantation. Methods The model of guinea pig to rat discordant liver xenotransplantation was set up. The orthotopic liver transplantation model was established by using modified two-cuff technique. Following groups were designed: control group, MSCs group and hIL-10MSCs group. Before and after operation, the recipients in control group were injected with normal sodium (2 ml) and decaesadril (0.5 ml) ;Those in MSCs group were injected with MSCs (4.0× 106/ml)and decaesadril (0.5 ml); Those in hIL-10-MSCs group were injected with hIL-10-MSCs (4.0 × 106/ ml)and decaesadril (0.5 ml). Twelve h after operation, the livers of recipient were observed by HE staining and electron microscope, and apoptosis of the livers was detected by using TUNEL. The expression levels of hIL-10, caspase-3, Fas and FasL were assayed by Western blot or immunohistochemistry. Results As compared with control group and MSCs group, the pathological changes was alleviated, the expression of IL-10 was significantly increased, the expression of FasL was significantly reduced in hIL-10-MSCs group. The positive area size of Fas and Caspase-3 in hIL-10MSCs group was 11.5 % and 25.1 %, respectively, significantly smaller than those in control group (35.3 % and 70.8 % respectively, P＜0.05. Apoptosis index in hIL-10-MSCs group was 32.5 %,which was significantly lower than in control group (74.1%) and MSCs group (50. 3 % ). ConclusionhIL-10-MSCs can notably decrease apoptosis of the transplanted liver probably by inhibiting theexpression of Fas/FasL.

Objective To observe the influence of hIL-10-MSCs on rejection of rat discordant liver xenotransplantation. Methods The model of cavia to rat discordant liver xenotransplantation was established. The rats were randomized into the control group, MSCs group, and hIL-10-MSCs group.The survival time and liver function of each recipient were observed and expression levels of E-Selectin, LFA-1, VCAM-1, and NF-κB determined by RT-PCR and ELISA 24 h after transplantation.Results The survival time was prolonged, liver function improved, and expressions levels of E-Selectin, LFA-1, VCAM-1 and NF-κB were significantly decreased in the hIL-10-MSCs group (P＜0.05).Conclusion hIL-10-MSCs are able to protect transplanted liver and decrease the expression of E-Selectin, LFA-1, VCAM-1, and NF-κB in the liver.

Objective To observe the effects of hIL-10-MSCs on heterologous T cell prolifera-tion. Methods hlL-10 was cloned by RT-PCR and then hIL-10/pLOX-cwGFPs was construct. The lentivirus was transfected into cavy MSCs and cell culture supernatant was tested for IL-10 protein by ELISA assay. The effects of hIL-10-MSCs on heterologous T cell proliferation were determined by CCK-8. The effect of peripheral blood mononuclearcell secretion IFN-γ was tested by EIASA. Results hIL-10/pLOX-cwGFP were constructed successfully and hIL-10-MSCs cell culture supernatant of IL-10 protein were increased obviously, hIL-10-MSCs could inhibit heterologous T cell proliferation sig-nificantly(P<0.05) but could not induce T cell proliferation (P<0.05). Adding IL-2 could reverse this inhibition(P<0.05), hIL-10-MSCs cell culture supernatant could inhibit peripheral blood mono-nuclearcell secretion IFN-γ. Conclusion hIL-10-MSCs may play an important role in significant im-munosuppression of heterologous T cell proliferation and suppression of secretion IFN-γ by peripheral blood mononuclear cells.

The anti tumor and immune activity of Algae Compound Preparation were studied in this paper. The results showed that Algae Compound Preparation could inhibit the growth of S 180 tumor planted to mice with the inhibition rate of 37.98%～50.38%. The life of ascites tumor mice were also prolonged with the prolongation rate of 21%～51.33%. At the same time the activity of apleen cells and the phagocytosis activity of macrophage were also enhanced.

Objective　To evaluate the disc herniation by the technique of MRI FFE.Methods　T1WI,T2WI and FFE sequences were performed in 40 cases with cervical and lumbar vertebra disc herniation using 1.0T MRI equipment on sagittal and transection sections.The showing rate of disc fibrous circle rupture,extrusion directon and nerve-root pressure in disc herniation on FFE,T1WI and T2WI sequences were comparated emphasisly.Resilts　In the observation of cervical nucleus pulpous prolapse and free disc fragment showing fibrous circle rupture,extrusion direction and nerve-root pressure in cervical region,and showing fibrous cicrcle rupture in lumbar region,the FFE was better than T1WI,T2WI.In judging extrusion direction and nerve-root pressure,the FFE sequence was better than T2WI and T1WI respectively.Conclusion　MRI FFE can improve the diagnostic ratio of the extrusion of nucleus pulpous,annular bulge as well as free disc fragment.

Objective To investigate the changes of serum electrolytes and blood coagulation function in the operative patients after massive blood transfusion.Methods Serum electrolytes and coagulation function during and after blood transfusion were de-tected and analyzed in 25 cases of massive blood transfusion.Results After intraoperative transfusion of blood products 1000mL, there were no significant changes in PT,APTT,TT and K+ ,Na+ ,Cl- ,Ca2 + (P >0.05),but after transfusing more than 3 000 mL of blood products,PT,APTT and TT were significantly prolonged(P <0.01),at the same time,the level of FIB was decreased ob-viously,K+ and Ca2 + were decreased obviously(P <0.01 ).Conclusion The patients with massive blood transfusion are prone to appear low potassium,hypocalcemia and coagulation function disorder.So the various indexes should be dynamically monitored dur-ing operation for avoiding the occurrence of serious complications.

ObjectiveTo evaluate reliability and psychiatric clinical value of quantum resonance spectrometer (QRS) application in depression symptoms detection.MethodsThe psychiatric symptoms of 97 cases respectively obtained from QRS test and psychiatrist check were performed comparative analysis.The detecting order and checking order was under an order of go to see doctor random.ResultsSensitivity and negative predictive value of QRS were 100％ same as psychiatrists'examination in eating disorders; also specificity and positive predictive were 100％ in depressed mood,will weaken and sleep disorders.Kappa values were all greater than 0.8 of QRS in slow thinking,depressed mood,will weaken and other symptoms.The AUC of ROC line were all greater than 0.9 of QRS in slow thinking,depressed mood,will weaken,et al.11 symptoms.ConclusionQRS could be used as secondary depression diagnostic tools,provide a new basis for clinical diagnosis.