It summarized the concept and the measurement of human caring, and introduced the concrete embodiment of human caring from three aspects. It analysed mainly the influence factors of human caring in China and put forward some thinkings and suggestions, in order to offer basis for nurse manager in carrying out human caring in future.

Objective:To evaluate the accuracy of YLM-Ⅱ quick Cefazolin Sodium Skin Tester(Made in Guangzhou WeiJeiHua Tech.Develop Co.,Ltd.)Methods:Use same batch number of Cefazolin Sodium on a person,at the same time do traditional skin test on the same person to compare the difference of the two methods.Results:Among the result of skin test on 267 patients,1 patient is positive in both methods,263 patients are negetive in both,3 patients got false positive in traditional skin test but negetive in trail means,none got negative in the traditional skin test while positive in trail means.Conclusion:Comparing the traditional skin test method with YLM-Ⅱ skin-test apparatus,the latter is more accurate,painless,time-saver and efficient.

Objective: To comprehend the route of infection and clinical manifestation of AIDS in my division.Methods: Analyze the 6 case investigations of AIDS in our division since August,2005.Result: 5 of them come from Chongqing,1 in Sichuan,most infected by domestic sexual contact;The common symptoms are repeated remittent fever?coughing?expectoration?shortness of breath? leukoplakia in mouth?rapid weight loss?striking tabefaction and so on.Conclusion: The epidemic situation of AIDS in Chongqing is rdpidly increasing.It is urgent to prevent and control the epidemic of AIDS in common people.

Objective To understand the needs and suggestions of respiratory medicine specialist nurses training, in order to provide some basis for the establishment of training mode of respiratory medicine specialist nurses. Method Using objective sampling method, we interviewed 12 students of different seni-ority , qualifications , title in the first session of training of nurse specialist in department of respiratory medicine of Chongqing, using qualitative research methods to analyze the data of the interview. Result Respondents had unified opinion on the curriculum setting, conditions of the training base, the requirements of core competence of junior nurses in department of respiration, but there existed differences about the length of service of the students . And the length of service of the teachers needed further discussion . Conclusion The opinions of the respondents collected provide reference for the establishment of training mode of nurse specialist in department of respiratory medicine. However, the students' opinions on the estab-lishment of the training standards are not uniform, which is worth further discussion.

Objective Through discussing application of case analysis to improve the comprehensive quality of nursing staff,and promote their implementation of high quality nursing care for the patients.Methods all 61 nurses in general wards were trained by daily half-hour case analysis method.After the training,questionnaire was given to investigate the nursing staff's evaluation on case analysis training,and at the same time compared before and after the training,the patients' satisfaction survey result,the monthly examination result of the nursing department,nursing paper quality and the number of nursing adverse events.The relevant data was done by t test orx2 test.Results After the implementation for half an hour case analysis training,most nurses think their own various ability was improved.After the training,the patient satisfaction was (t=-0.391),the monthly assessment score of the nursing department(t=3.823),the quality of care increased(x2=5.495),while the nursing adverse events reduced(x2=6.441),and the difference was statistically significant compared with before the training(P＜0.05).Conclusion Daily half-hour case study method can improve the nursing staff comprehensive quality and scientific research ability,the patients' satisfaction,and reduce the nursing adverse events,which is helpful to clinical practice.

ObjectiveTo explore the effect of institutionalized humanistic care on relieving patients' anxiety.MethodsTotally 424 patients with anxiety in respiratory department were randomly divided into experiment group ( n=198 ) and control group ( n=226 ).The institutionalized humanistic care was used in experiment group accompanied by normal holistic nursing while only holistic nursing was used in the control group.The anxiety status of patients was evaluated by using the hamilton anxiety scale ( HAMA ) from admission to discharge.The relief of anxiety was compared in two groups.Results①The seores of two groups were decreased because of the intervention ( P ＜0.01 ).②The scores of anxiety in experiment group was lower than that in control group at discharge ( P ＜0.01 ).ConclusionThe anxious emotion of patients was relieved after the intervention in the hospital,however,institutionalized humanistic care is more effective in relieving anxiety in patients.

Objective: To prepare auto-tumor-specific cytotoxicity T lymphocytes (CTLs) of breast cancer patients and to observe their therapeutic effects on bone marrow micrometastasis (BMM) of breast cancer. Methods: BMM in 82 patients with primary breast cancer (stage Ⅰ to Ⅲ) , who were treated in the Fourth Affiliated Hospital of Hebei Medical University from March to December in 2007 (all the patients signed paper of informed consent), was exmined by flow cytometryusing CK18 and CK19 as marker. Twenty-three patients with BMM were randomly divided into two groups: 17 patients were treated with tumor-specific CTLs (therapy group), and 6 patients were treated with IL-2 (control group). Tumor-specific CTLs were induced in vitro from axillary lymph nodes and peripheral blood of breast cancer patients in ther-apy group, and were reinfused into the same patient 10-14 days after operation. The therapeutic effects of tumor-specific CTLs on BMM of breast cancer patients were observed. Results: Twenty-three cases (28.05%) in 82 breast cancer patients were BMM positive as detected by FCM. BMM positive rates increased with the increase of clinical TNM stages and histological grades of breast cancer, and decreased with the increase of ER and PR protein expression in cancer tis-sues. Dendritic cells (DCs) were successfully isolated and induced from the peripheral blood of breast cancer patients. Tumor-specific CTLs were induced by co-culturing lymphocytes from axillarey lymph nodes with auto-tumor antigen-im-pulsed DCs. Fourteen cases in the therapy group became negative of BMM after treatment with tumor-specific CTLs (14/ 19, 82.35%). Only one case in the control group became negative of BMM after treatment with IL-2 (1/6, 16.67%, P=0.00028). Conclusion: Tumor-specific CTLs have been successfully prepared and they show a satisfactory therapeu-tic effect on bone marrow micrometastasis of breast cancer.

Objective To thoroughly explore the pathophysiological roles of TIMP-1 during the progressive course of renal diseases, the study was aimed at identifying the functional phenotype of endogenous and exogenous genes in kidneys of human TIMP-1 transgenic mice. Methods Renal histological changes between 3-month-old wild type mice (n=8) and 3-month-old transgenic mice (n=8) were analyzed through PAS staining of paraffin sections. The mRNA and protein expressions of h/mTIMP-1, mTIMP-1, TIMP-2, TIMP-3, MMP-9, MMP-2, and COLⅣ?5 mRNA were detected by Northern blot and Western blot. The activities of gelatinases and TIMP-1 were examined by gelatin zymography and reverse zymography, respectively. Results No difference in histological picture in kidneys was found between wild type and transgenic mice. In contrast with wild type mice, it was found that in kidneys of transgenic mice, the mRNA and protein expressions of h/mTIMP-1 and its activity were up-regulated (P0.05). Conclusion The transgene was expressed steadily in kidneys of human TIMP-1 transgenic mice, and it induced the compensation of MMPs/TIMPs.

Objective To study the effects of using the nursing intervention during the course of cure patients with depression after the stroke. Methods Divided 127 patients with depression after stroke into the treatment group(63 cases) and the control group(64 cases) randomly,the general nursing intervention and the traditional nursing measures were adopted respectively in the two groups.Compared the effects of treatment between the two groups by the questionnaires. Results Results The effects of treatment in the treatment group were significant better than those of in the control group. Conclusion The general nursing intervention can effective improve the depression of patients with stroke,and then ameliorate their quality of life,shorten the time of recovery.

Objective To explore the humanistic nursing care for the impact on the overall quality of care.Methods 1000 patients were randomly divided into experimental and control group,traditional holistic nursing was implemented in the control group,while both traditional holistic nursing and humane care were implemented in the experimental group to compare the differences of patient satisfaction by questionnaire survey.Results The humane care group was significantly higher in patient satisfaction,and the differences are statistically significant(P