Objective To evaluate the myocardial microvascular obstruction induced by occlusion of single coronary artery (OSCA) and stenosis of multi-coronary arteries (SMCA) using first-pass perfusion cardiac MRI. Methods We studied 33 patients who had acute myocardial infarct and received emergency coronary angiography and angioplasty. Coronary angiogram confirmed that 15 patients had complete occlusion of the single coronary artery and 18 patients had multi-coronary artery stenosis without occlusion. Cardiac MRI (CMRI) exam was performed at 6-12 weeks after diagnosis. Results CMRI demonstrated that 15 patients had first-pass perfusion defect in the endomyocardium. The signal intensity (SI) of the perfusion defect was significant lower than that in the normal myocardium. The maximal slope of SI showed that the there were 11 patients with occlusion of the single coronary artery and 4 patients with stenosis of multi-coronary arteries. Conclusion CMRI can show the microvascular obstruction limited in the region of endomyocardium. CMRI suggests that the occlusion of the single coronary artery may induce more severe myocardial microvascular obstruction than the stenosis of multiple coronary arteries.

PurposeDiffusion weighted imaging (DWI) can significantly improve the diagnosis of non-enlarged lymph node metastasis. This study aims to evaluate the correlation between the DWI findings and the prognosis, and to identify prognostic factors.Materials and Methods Forty-seven patients with colorectal cancer underwent MRI scan including DWI sequence before surgery. Imaging ifndings were compared with the pathologic results to determine the metastatic lymph nodes (DWI positive) or non-metastatic lymph nodes (DWI negative). Postoperative disease-free survival and overall survival for 5 years of the patients with DWI positive and DWI negative lymph nodes were compared. Correlation between the prognosis and the related factors were investigated including regional DWI-positive lymph nodes, short axis diameter and long axis diameter of the largest DWI-positive lymph node, and number of DWI-positive nodes.Results Of 47 patients,10 (21%) patients had regional DWI-positive lymph nodes showed high signal intensity on diffusion-weighted images. The patients with regional DWI negative lymph nodes had a signiifcant better ifve-year disease-free survival and overall survival (P<0.05). The short axis diameter of the largest DWI-positive lymph node was correlated with distant metastasis (AUC=0.77,P<0.05). The short axis diameter and long axis diameter of the maximum metastatic lymph nodes were correlated with overall survival (AUC=0.84 and 0.75,P<0.05). Five-year disease-free survival and overall survival of the patients with short axis diameter of the largest DWI-positive lymph node ≤9 mm were higher than the patients with lymph node short axis diameter >9 mm (P<0.05). Five-year disease-free survival and overall survival of the patients with long axis diameter of the largest DWI-positive lymph nodes ≤11 mm were higher than the patients with long axis diameter >11 mm (P<0.05). Five-year disease-free survival of the patients with all DWI positive lymph nodes resected was higher than the patient without DWI-positive lymph nodes resected (P<0.05).Conclusion The patients with regional DWI-negative lymph node had a better prognosis. Of the patients with DWI-positive lymph nodes, the patients with smaller lymph nodes have better prognosis than who have larger lymph nodes.

Objective To compare the diagnosic performance of conventional 2D and 3D Cube MR imaging to identify complete and partial tears of anterior cruciate ligament(ACL).Methods Farty patients suspected of having tears of anterior cruciate ligament were prospectively studied by 3.0 T conventional 2D and 3D Cube MR imaging and arthroscopy.MR images were interpreted in consensus by two experienced radiologists,and the ACL was diagnosed as being normal,partially torn,or completely torn.Diagnostic accuracy of 2D and 3D Cube MR for the detection of both complete and partial tears of ACL was calculated using arthroscopy as the standard of reference.Area under curve (AUC) of both methods were calculated using ROC curves and were compared using Hanley& McNeil curve comparisions.Results Sixteen patients had intact ACL,12 had complete tear,and 12 had partial tear of the ACL at arthroscopy.For complete ACL tear,AUC of 2D MR and 3D Cube was 0.839 and 0.923 respectively,and there was no significant difference on ROC curves (Z =1.245,P =0.213).For partial ACL tears,AUC of 2D and 3D Cube MR were 0.643 and 0.881 respectively,and there was significant difference on ROC curves (Z =2.384,P =0.017).Conclusions Both 2D MR and 3D Cube MR have high sensitivity and specificity for identifying the complete ruptures of ACL.3D Cube MRI appears to be superior inidentifying partial rupture of ACL.

Objective To evaluate the diagnostic value of MRCP compared with ultrasound in patients with cholangiopancreatic disease by ROC curve. Methods The data of 62 patients with suspected cholangiopancreatic disease undergoing both MRCP and ultrasound were retrospectively studied. ROC analysis was performed by ROCKIT software. Results Area under the ROC curve of MRCP was 0.739. Area under the ROC curve of US was 0. 930. ROC analysis showed that these two modalities were effective in diagnosing cholangiopancreatic disease, with MRCP being more effective than ultrasound, especially for the diagosis of lower biliary tract diseases. Conclusion MRCP combined with MRCP source images is of great help in the diagnosis of pancreaticobiliary duct diseases.

Objective To study the metabolic alteration in the brain of patients with cyanosed congenital heart disease (CCHD) by using 1H magnetic resonance spectroscopy ( 1H MRS) and discover the pathophysiology of chronic hypoxic brain, which will help to diagnose and treat this disease completely. Methods Twenty five patients with CCHD and 25 controls were performed PRESS 1H MRS and MRI, The areas under the resonance of metabolites were measured, the ratios of the other metabolites to Cr were calculated and compared. Results In patients with CCHD, the mean value of NAA/ Cr was significantly lower than that in controls ( P 0.05). Conclusion 1H MRS can detect brain metabolic changes in patients with cyanosed congenital heart disease in vivo noninvasively and can detect the metabolism disorder of the energy and amino acid, so the pathophysiology of this disease can be understood.

Objective To explore the X ray imaging of the common complications after total hip replacement (THR), in order to improve the diagnosis and differential diagnosis of the complications of THR. Methods Forty six cases were revised because of the complications of THR. The value of X ray in the diagnosis of complications was assessed by observing the radiography and comparing with surgical results. Results In these cases, after revision, 34 cases had aseptic loosening of femoral component, 13 cases had aseptic loosening of acetabular component, presenting on radiographs as the lucent zone alone the component, which was ≥2 mm in width and had the same contour as the component. 4 cases had septic loosening of femoral and (or) acetabular component, presenting on radiographs as the lucent zone alone the component, and bone destruction and sinus were also observed. The lucent zone was also ≥3 mm, but had the different contour of the component. Both kinds of loosening may accompany with migration of the component or not. 6 cases had dislocation of femoral head, presenting on radiographs as the head lying out of acetabulum in 2 cases, central migrating of the head in 2 cases, and asymmetrical position of the head in acetabulum in 2 cases. Conclusion The lucent zone and component migration had the important diagnostic value of loosening, the contour of lucent zone and sinus could help to make the differential diagnosis of aseptic and septic loosening. Asymmetrical position of the head in acetabulum and the head lying out of acetabulum were the signs of dislocation.

ObjectiveTo improve the visualization of anteromedial and posterolateral bundles of anterior cruciate ligament (ACL) and investigate the optimal MRI plane for the bundles at a 3.0 T MR scanner.MethodsMR images of 149 knee joints were reviewed retrospectively.Display rates of AMB,PLB and their different parts (the top portion,the middle portion and the low portion) on MR different planes including axial,sagittal and coronal planes were analyzed and their differences were compared with the x2 section method.ResultsThere was no statistical difference in the display rates of two bundles of ACL between axial plane ( 115/149,77.2％ ) and coronal plane (103/149,69.1％ ) (x2 =2.4606,P ＞0.0125 ).Statistical differences were found between axial and sagittal plane,coronal plane and sagittal plane (21/149,14.1％ ) ( x2 =119.5138,92.8695 respectively,P ＜0.0125 ).There was a statistical difference for the top portion of ACL between axial plane ( 104/149,69.8％ ) and coronal plane,sagittal (0/149,0)and coronal planes ( 7/149,4.7％ ) ( x2 =135.081,159.7526 respectively,P ＜ 0.0125 ),between sagittal and coronal planes (x2 =7.1684,P ＜ 0.0125 ).For the middle portion of ACL,there was no statistical difference between axial plane ( 108/149,72.5％ ) and coronal plane (94/149,63.1％ ) (x2 =3.0120,P ＞ 0.0125 ),while statistical differences were found between axial and sagittal plane,coronal planes and sagittal plane ( 10/149,6.7％ ) ( x2 =134.7454,104.2173 respectively,P ＜ 0.0125 ).For the low portion of ACL,there was no statistical difference between axial plane ( 103/149,69.1％ ) and coronal plane (101/149,73.8％ ) (x2 =0.8065,P ＞0.0125),while statistical differences were detected between axial and sagittal plane,coronal planes and sagittal plane ( 18/149,12.1％ ) ( x2 =100.5300,115.9132,P ＜ 0.0125 ). The different parts of ACL displayed low intensity on different MR planes and normal morphology.ConclusionsACL can be displayed on conventional MR planes at a 3.0 T MR scanner to some extent.Axial and coronal planes might be the optimal MRI planes for ACL and its two bundles.

BACKGROUND:Bone marrow mesenchymal stem cells have the chemotaxis and homing role that promotes immune system reconstruction, eliminate residual lesions and prevent recurrence in patients.
OBJECTIVE:To observe therapeutic effect of human bone marrow mesenchymal stem cells transplantation into high-metastatic potential hepatocellular carcinoma animal models on metastatic potential of high-metastatic potential hepatocellular carcinoma.
METHODS:Nude mouse models of high-metastatic potential hepatocellular carcinoma were established in vivo. In the experimental group, 5×105 cells were injected via the tail vein on day 7 after tumor inoculation, twice a week. In the control group, cellculture medium, 0.2 mL per mouse, was injected by the tail vein. After the start of the experiment, tumor volume was measured every 4 days. After tumor inoculation for 14 days, 21 days, 28 days, 35 days, 42 days, animal models were sacrificed, and then tumor mass and body mass were recorded to calculate the inhibition rate. PCR was employed to detect osteopontin, bone sialoprotein, and integrinα Ⅴ mRNA expression, as wel as bcl-2, bax, caspase3 mRNA expression.
RESULTS AND CONCLUSION:The inhibition rate of tumor mass showed the best results in week 3. As time went on, the tumor inhibition rate was gradual y decreased. Metastasis-related biological factors showed a gradual down-regulated trend, indicating the polarization of tumor apoptotic indexes, that is, anti-apoptotic factor, bcl-2, showed a decreasing trend, while apoptotic factors, bax and caspase3, appeared to have a gradual y increased trend. These findings suggest that human bone marrow mesenchymal stem cells effects to inhibit high-metastatic potential hepatocellular carcinoma animal models appear to vary with time. After human bone marrow mesenchymal stem cells transplantation for 3 weeks, the inhibition performance on high-metastatic potential hepatocellular carcinoma is the best, and then it weakens with time. Human bone marrow mesenchymal stem cells are found to inhibit the metastatic potential of hepatocellular carcinoma.

Objective To develop a Gd-based MR probe containing arginine-glyeine-aspartic acid (RGD)motif to reveal integrin avβ3 receptor-expressed tumor.Methods Commercially available HYNICRGD conjugate with co-ligand EDDA was labeled with GdCl3,and the mixture was isolated and purified by solid phase extract(SPE)to get the entire probe Gd-EDDA-HYNIC-RGD.Human HCC cell line BEL-7402 was cultured and the cells harvested and suspended then subcutaneously inoculated into athymic nude mice for tumor growth.In vitro cell binding assay to integrin avβ3 receptor and cell viability experiments were conducted.Then in vivo,imaging of the three arms of xenografts were performed by MR scan with a dedicated animal coil at baseline and time points of 0.30,60,90 mninutes and 24 hour post-intravenous injection(P.i.) via the tail vein.Three arms of nude mice then were sacrificed for histological examination to confirm the imaging results.Results Gd-EDDA-HYNIC-RGD was successfully isolated by SPE and validity was verifled on signal enhancement througll in vitro and in vivo experiments.The T1 relaxation rate of the probe is 3.31 mmol/s:It is well tolerated to living cells when the concentration of the probe is below 0.1 μmol/ml;both BEL-7402 Hunlan Hepatocellular Carcinoma cell Iine and the tumor expressed avβ3receptor;The RGD-iigand was observed specificly binding with avβ3 receptor in vitro;The nude mice model bearing HHCC was well estabhshed.The signal intensity(SI)at the tumor site were 2247.6±39.0 at baseline and 2820.9±35.2 at 90 min p.i.respectively,the SI at 90min increased less than 25%of baseline,which is statistically different(t=-38.031,P＜0.05);while the SI at muscle site were 1824.2±32.8 and 1845.8±27.2 respectively,which is not statistically different(t=-1.424,P＞0.05);The signal to time curve for probe-administrated group is straightforward over time in the span of 0 to 90 minute p.i.while the control arms do not show such tendency.Conclusion Gd-EDDA-HYNIC-RGD has the potential to used as an MR probe detecting integrin avβ3 receptor-expressed tumor.This work may offer possibility of early detection and differentiation of specific tumors.

Objective To evaluate the possible white matter damage and to define the location of the damage in Alzheimer′s disease (AD) with diffusion tensor imaging (DTI). Methods Twenty-four AD patients and twenty-one age-matched healthy volunteers received conventional and DTI scanning.The ADC and FA of white matter in temporal, parietal, frontal lobe and cingulum were measured respectively and the data underwent postprocessing.Results FA value of the whiter matter in frontal, parietal, temporal and cingulum in AD patients was 0.37?0.06, 0.32?0.05, 0.26?0.03, and 0.47?0.09, respectively, and ADC value was 9.50 e-10?2.02 e-10, 10.55 e-10?1.43 e-10, 11.45 e-10?0.76 e-10, and 10.10 e-10?2.18 e-10, respectively;FA value of the same corresponding regions in control was 0.44?0.06, 0.38?0.05, 0.32?0.05, and 0.56?0.06, respectively, and ADC value was 8.75 e-10? 1.63 e-10, 9.83 e-10?0.99 e-10, 11.13 e-10?0.78 e-10, and 8.28 e-10?1.65 e-10, respectively.FA value of the whiter matter in frontal, parietal, temporal, lobe and cingulum decreased (P≤0.006) and ADC value increased in cingulum white matter (P≤0.006) in AD patients.Conclusion DTI could reveal the damage in white matter of frontal, temporal, parietal lobe and cingulum.It suggested that not only the gray matter is injuried, but also the white matter is abnormal in AD patients.