Objective To evaluate the effect of low-dose omeprazole in the treatment of functional dyspepsia(FD).Methods A total of 260 FD patients were diagnosed clearly and divided into two groups randomly:148 cases(observation group)were treated with low-dose omeprazole(10 mg/d),while 112 cases(control group)were treated with esomeprazole(20 mg/d)and domperidone(each 10 mg,three times per day).Two groups were all treated for 2 weeks.The clinical symptoms such as heartburn,postprandial distenstion,epigastric pain and eructation were observed.Results Compared with symptoms in two groups before treatment,the scores of heartburn and epigastric pain were decreased evidently after treatment in two groups(P

OBJECTIVE:To explore the effects of Haikangling Mixture on immunological function in immunosuppressed mice induced by cyclophosphamide. METHODS: Mice were given Haikangling Mixture intragastrically for 14d, and the level of hemolysin was determined by sheep erythrocyte (SRBC) immunization to exploer the influence of Haikangling on humoral immunity in mice; splenic lymphocytes were measured by MTT to observe the influence on cell immunity; the clearance of macrophage were measured by carbon particle clearance method to explore the influence on non-specificity immunity. RESULTS: The level of hemolysin, the cell reproductive activity, splenic lymphocytes and the phagotrophy of mononuclear macrophage were improved obviously by Haikangling Mixture in immunosuppressed mice induced by cyclophosphamide. CONCLUSION: Haikangling Mixture can enhance the immunological function in immunosuppressed mice induced by cyclophosphamide.

Objectives To investigate the diagnostic value and safety of needle cutting biopsy for the primary and metastatic hepatic cancer during the treatment of percutaneous radiofrequency ablation and ethanol injection guided by ultrosound or CT. Methods The clinical data of 48 patients underwent the treatment of percutaneous radiofrequency ablation and ethanol injection as well as biopsy were analyzed. Results Among 48 patients,46 case(95.8%) were diagnosed by pathological examination and 2 cases (4.2%) failed to be diagnosed. There were no serious complications in all the patients. Conclusion Detachable needle cutting biopsy was safe and effective for the diagnosis of the primary and metastatic hepatic cancer during the treatment of percutaneous radiofrequency ablation and ethanol injection guided by ultrasound or CT.

Objective To study the effects of simulated gravity loss on nuclear factor Kappa-B expression in rats′ pulmonary tissue, and to investigate the mechanism of changes in NF-?B during simulated loss of gravity. Methods Tail-suspension(TS) was used as the method to simulate the physiological effects of weightlessness. 40 Wistar male rats Wistar rats were randomly divided into 4 groups: control for 7 days (7d CON), 21d CON, TS for 7d (TS7d) and TS21d. The dynamic express of nuclear factor Kappa-B in lung tissue was respectively assessed using the immunohisochemical technique. Results Compared with control group, the expression level of NF-?B in the lung tissue of tail-suspension 7d rats was elevated significantly (P

Objective To study the pain relief effectiveness of the combined spinal epidural analgesia(CSEA) and the inhalation of nitrous oxide, and the influences on the mothers and infants Methods The 300 cases of pregnant women were randomly divided into 3 groups: CSEA group,nitrous oxide group and control group The nitrous oxide group was that pregnant women inhaled nitrous oxide premixed with oxygen (50%∶50%),the pregnant women of the CSEA group were injected fentanyl and bupivacaine in the subarachnoid and epidural space,analgesic was not used in the control group The degree of labor pain, duration of the labor,way of delivery, bleeding volume, rate of anoxia of newborn,blood gas analysis to maternal radius artery and fetal umbilical blood among 3 groups were observed Results The effect for analgesia labor of the CSEA group was much better than that of the nitrous oxide group ( P 0 05) In the second stage of labor,the 3 groups were alike to each other The bleeding volume of caesarean section (373?77) ml in the nitrous oxide group was much more than the other 2 groups, there was no difference between the CSEA group (259?78) ml and the control group (239?89) ml The rate of obstetric forceps of CSEA group was higher than the control group ( P

Objective To investigate the neuroprotective potentials of recombinant human erythropoietin ( rhEPO) in premature rats with white matter damage. Methods Pregnant rats ( gestational age 15 days) were injected lipopolysaccharide (LPS) (300 μg/kg) intraperitoneally to make cerebral white matter lesions. Another 10 cases of pregnant rats were injected saline (1 mL/kg) intraperitoneally as controls. All preterm rats were born by caesarean sec-tion on embryonic day 21. According to the different processing method,the preterm rats were randomly divided into LPS+rhEPO group (18 cases),LPS+ normal saline (NS) group (18 cases),NS+rhEPO group (18 cases) and NS+NS group (18 cases) ,and they were injected rhEPO (5 000 IU/kg) or 9 g/L saline 1 mL/kg intraperitoneally imme-diately after birth,respectively. The cerebral white matter injury was evaluated with HE staining,and levels of CD68 ,gli-al fibrillary acidic protein (GFAP),myelin basic protein (MBP) were detected by immunofluorescence method 3 and 7 days after birth. Assessment of nerve behavior was done 2 weeks after birth. Results HE staining showed that the white matter lesions were less in LPS+rhEPO group than those in LPS+NS group 3 and 7 days after birth,while NS+rhEPO group and NS+NS group had no cerebral white matter lesions. The expressions of CD68 in LPS+rhEPO group,NS+rhEPO group,NS+NS group significantly decreased compared with LPS+NS group (F=7. 456,P<0. 01) 3 days after birth. The expressions of GFAP in LPS+rhEPO group,NS+rhEPO group,NS+NS group were lower than those in LPS+NS group (F=5. 121,P< 0. 01) 7 days after birth. Meanwhile,the expressions of MBP were not statistically different from the other groups 3 and 7 days after birth (F=2. 628,1. 425,all P>0. 05). No significant differences were found between LPS+rhEPO group and the other groups in evaluation of long-term neural development(all P>0. 05). The val-ues of F by the open field test,suspension test,slope hill test,and resistance to capture test were 2. 09,0. 53,0. 11,0. 37, respectively. Conclusions A single large dose (5 000 IU/kg) rhEPO has neuroprotective effect on the cerebral white matter lesions in the premature rats by inhibiting microglia and astrocyte activation in a short time. The long-term effort remains unknown.

ObjectiveTo evaluate left ventricle partial and global diastolic function in patients with silentmyocardial ischemia (SMI) by Doppler tissue imaging (DTI) combined with dobutamine stress echocardiography(DSE).Methods30 cases of silent myocardial ischemia(SMI) and 30 cases of normal control underwent dobu-tamine stressechocardiography(DSE). DTI was acquired at baseline and stress, and waveforms in mitral valve clos- ing, and the base and mid segments of left ventricle were used to measure diastolic early velocity(Ve), diastolic latelyvelocity(Va) ,and E deceleration time(Te). ResultsⅤ in control group increased accompanied with the increasingdose of dobutamine, Ve/Va decreased a little but not significantly. Ⅴ in the group with silent myocardial ischemia(SMI) increased at 10μg·kg-1·min-1 ,but decreased at 40μg·kg-1·min-1 compared with 10μg·kg-1·min-1 ,T inboth control and DSE group decreased at 40μg/kg accompanied with 10μg·kg-1·min-1, T in both control and DSEgroup decreased accompanied with the increasing dose of dobutamine, and there were significant differences betweenthe groups(P < 0.05). ConclusionDTI-DSE could quantificadvely detect the partial and global diastolic function ofleft ventricle.

Objective To evaluate the parameters of inner diameter different rate (D%), mean wave intensity(MWl) and diameter-adjusted mean wave intension(DAMWI) in assessing the function of common carotid artery(CCA) by quality intima-media thickness(QIMT) technique. Methods Twenty-four healthy subjects were performed 2D and CDFI examination under the normal quiescent and cold pressor test(CPT) condition respectively. The diastolic and systolic diameters of left-CCA were measured by QIMT respectively. Corresponding the inner diameter different range,rate,MWI and DAMWI were calculated respectively. The curves of their ROC were drawn. Results The different range and rate of diameters were decreased when subjects suffered CPT (P< 0.05) ,but the MWI and DAMWI were increased (P<0. 05). The areas under the ROC curves were 0. 827,0. 821 and 0.728 corresponding DAMWI, MWl and different rate of diameter. There was no significant difference among them(P>0. 05). Conclusions Conclusions The changed rate of diameter can be measured exactly by QIMT. The function of CCA can Be evaluated by MW1 and DAMWI which were calculated by conventional parameters.

A high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method was developed and validated for the determination of mizoribine in human serum using thiamphenicol as internal standard (IS). The serum samples of mizoribine were precipitated with acetonitrile and separated by HPLC on a reversed phase C18 column with a mobile phase of 0.1% ammonium acetate water solution-methanol (47:53, v/v). Mizoribine and IS were detected in the multiple reaction monitoring mode with precursor/product ion transitions of m/z 258.2/126.0 and 354.1/185.2, respectively. The calibration curves were linear over the range of 0.02-2 microg mL(-1) for mizoribine. The limit of quantification (LOQ) was 0.02 microg mL(-1) with acceptable precision and accuracy. The validated method was successfully applied for the evaluation of a bioequivalence study on Chinese healthy volunteers. The main pharmacokinetics parameters after oral administration of 100 mg mizoribine test or reference formulation were as follows: Cmax (1.00 +/- 0.21), (1.00 +/- 0.22) microg mL(-1); AUC(0-infinity) (6.72 +/- 1.39), (6.48 +/- 1.44) microg h mL(-1); t1/2 (2.77 +/- 0.26), (2.66 +/- 0.29) h; tmax (2.95 +/- 0.78), (2.84 +/- 0.50) h.